• Journal of the Korean Society of Radiology
• /
• v.10 no.7
• /
• pp.483-487
• /
• 2016

The purpose of this study is to investigate whether gold nanoparticles had radiosensitization when combined with gamma and x ray beam. Cytotoxicity was mearsured with comparing survival fraction after incubated time 6,12,18 and 24 hours. Clonogenic assay was employed to assess survival fraction of cells with and without gold nanoparticles treatment following gamma ray irradiation. The most of gold nanoparticles were distributed in the cytoplasm. And the toxicity of gold nanoparticles used this study were found to be non-cytotoxic. And we also observed enhancement by about 40% in RBE value for gamma ray irradiation of cells treated with gold nanoparticles. Dose reduction of about half for gamma ray irradiation is demonstrated for gold nanoparticles treated cells as compared to untreated cells. In cells with exposed to gamma ray, DNA damage was increased when compared to only radiation exposed cells. The study revealed a significant reduction in radiation dose for killing the cells with internalized gold nanoparticles as compared to the cells without gold nanoparticles. The gold nanoparticles treatment resulted in enhancement of radiation effect as evident from increase in relative biological effectiveness values for photon irradiated cells.

• Korean Journal of Materials Research
• /
• v.16 no.8
• /
• pp.524-528
• /
• 2006

On the synthesis of Au/$TiO_2$ core-shell structure nanoparticle, the effect of concentration of $Ti^{4+}$ and reaction temperature on the morphology and optical property of Au/$TiO_2$ core-shell nanoparticles is examined. A gold colloid was prepared by $HAuCl_4{\cdot}4H_2O\;and\;C_6H_5Na_3{\cdot}2H_2O$. Titanium stock solution was prepared by mixing solution of titanium(IV) isopropoxide (TTIP) and triethanolamine (TEOA). The concentrations of $Ti^{4+}$ stock solution were adjusted to $10.01{\sim}0.3$ mM, and then the gold colloid is added to the $Ti^{4+}$ stock solution. Au/$TiO_2$ core-shell structure nanoparticles could be prepared by the hydrolysis of the $Ti^{4+}$ stock solution at $80^{\circ}C$. The size of synthesized Au nanoparticles was 15 nm. The thickness of $TiO_2$ shell on the surface of gold particles was about 10 nm. The absorption peak of synthesized Au/$TiO_2$ core-shell nanoparticles shifted towards the red end of the spectrum by about 3 nm because of the formation of $TiO_2$ shell on the surface of gold particles. The good $TiO_2$ shell is produced when $Ti^{4+}$ concentration is varied between 0.01 and 0.05 mM, and reaction temperature is maintained at $80^{\circ}C$. The crystal structure of $TiO_2$ shell was amorphous.

• Journal of Biosystems Engineering
• /
• v.43 no.4
• /
• pp.394-400
• /
• 2018

Purpose: This research evaluated five types of nanoparticles to develop a surface-enhanced Raman spectroscopy (SERS) method for the rapid detection of two Bacillus species (Bacillus cereus and Bacillus thuringiensis) that are commonly found on fresh produce, which can cause food poisoning. Methods: Bacterial concentrations were adjusted to a constant turbidity, and a total of $30{\mu}L$ of each Bacillus cell suspension was prepared for each nanoparticle. A point-scan Raman system with laser light source of wavelength 785 nm was used to obtain SERS data. Results: There was no qualitative difference in the SERS data of B. cereus and B. thuringiensis for any of the five nanoparticles. Three gold nanoparticles, stabilized in either citrate buffer or ethanol, showed subtle differences in Raman intensities of two Bacillus species at $877.7cm^{-1}$. Conclusions: Among the three types of nanoparticles, the gold nanoparticles stabilized in citrate buffer showed the lowest standard deviation, followed by gold nanoparticles stabilized in ethanol. This result supports the potential application of gold nanoparticles for SERS-based detection of B. cereus and B. thuringiensis.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.4
• /
• pp.221-226
• /
• 2003

With the current rapid development of nanotechnology and synthesis technology for designed oligonucleotides or oligonucleotide-modified nanoparticle conjugates, the combined strategies have become one of the most valuable methods in detection technology for DNA analysis. Using the uniquely recognizable interactions of pre-designed DNA molecules in assembling nanoparticles, various novel approaches have been recently developed towards detecting specific DNA sequences. Here we describe the key fundamentals and issues of this promising strategies ranging from the initial findings of rationally designed DNA-based assembly of nanoparticles to the extended chip-based detection system. Some limitations of these new strategies and possible approaches will be also discussed for the practical application in the area of DNA microarray detection.

• Journal of Sensor Science and Technology
• /
• v.25 no.6
• /
• pp.435-439
• /
• 2016

We report a novel detection technology for glycated hemoglobin (HbA1c) that is measured primarily to identify the three-month average plasma glucose concentration. In enzymatic measuring of glycated hemoglobin, the generated hydrogen peroxide was then used as a reducing agent of gold (III) for the synthesis of gold (0). Gold nanoparticles obtained from this novel approach were measured by optical and piezoelectric methods. In optical method, we have developed polymer based film-type sensor cartridge filled with all the reagents for glycated hemoglobin analysis and the cartridge worked very well having the detection limit of 0.53% of glycated hemoglobin. On the other hand, quartz crystal microbalance (QCM) sensors also have been developed to determine the abilities of surface modified QCM sensors at various levels of the concentration of glycated hemoglobin to bind gold nanoparticles and limit of detection was 0.90%. Finally, despite of relatively lower sensitivities of QCM sensor and film-type optical sensor than well-plate based optical detection, these two sensors were available to measure the glycated hemoglobin level for diabetes patients and normal person.

• Advances in nano research
• /
• v.14 no.1
• /
• pp.17-25
• /
• 2023

Gold nanoparticles have recognized a promising drug carriers in many diseases. These nanoparticles could carry anti-inflammatory drugs in the case of muscle injury and for fatigue relief. On the other hand, specific surface of this kind of nanoparticles could be critical in amount of drug they could carry. Therefore, in this study, we explore different methodology and influencing parameters on the specific surface of gold nanoparticles. After specifying the main parameters, different machine learning and artificial neural network are adopted to model the effects of different parameters. Furthermore, response surface methodology is utilized to obtain a quadrilateral relationship between different parameters and specific surface. The results indicate that concentration of the gold salt solution is the most important parameter in increasing the size of gold nanoparticle and, as a consequence, increasing specific surface. Moreover, the ability of gold nanoparticles in prolonging retention of the drugs is discussed in detail.

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.493-493
• /
• 2005

• Proceedings of the Korean Fiber Society Conference
• /
• 2007.11a
• /
• pp.513-514
• /
• 2007

• Polymer Science and Technology
• /
• v.25 no.1
• /
• pp.15-20
• /
• 2014

• Bulletin of the Korean Chemical Society
• /
• v.24 no.5
• /
• pp.684-686
• /
• 2003