• 제목/요약/키워드: Glycine max (L.) Merr.

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Variation of Anthocyanin and Protein Contents in Glycine max L. (Merr) (Soybean) Germplasms from Korea

  • Choi, Yu Mi;Lee, Sukyeung;Hyun, Do-Yoon;Ko, Ho-Cheol;Rho, Nayoung;Hur, On-Sook;Yoon, Hyemyeong;Lee, Myung-Chul;Oh, Sejong;Shin, Myoung-Jae;DESTA, Kebede Taye
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 추계학술대회
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    • pp.13-13
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    • 2019
  • Soybean (Glycine max L. (Merr) is commonly consumed and found in major foods including soymilk, soy sauce, tofu, and soy sprout in Korea and east Asia. In addition, it is common to cook the whole seeds with rice. Soybean is known to have ranges of health benefits including antiaging, anticancer, neuroprotective and antidiabetic taken either as supplement or dietary food. Anthocyanins and flavonoids in G. max are found to be the main contributors to such wide arrays of health benefits. Due to increasing economic values of soybean, development of specialty soybean cultivars is becoming an area of interest worldwide. In this study, 746 black soybean accessions from National Agrobiodiversity Center were characterized as part of an attempt to identify important germplasms of G. max. Seed coats of each accession were analyzed for their total anthocyanin, cyanidin 3-O-Glucoside (C-3-O-G), delphinidin 3-O-glucoside (D-3-O-G), petunidin-3-O-glucoside (Pt-3-O-G), and their whole seeds for crude protein contents. HPLC was used to determine and quantify the anthocyanin compositions while crude protein was determined using Kjeldahl method by Kjeltec auto-analyzer (Kjeltec 8400, Foss, Sweden). Accessions were grouped according to their anthocyanins and protein contents; the mean content of which were correlated to agronomic traits including maturity date, one hundred seed weight, cotyledon color and seed lust color. The results indicated that the total anthocyanin content (TAC) ranged from 273.77 to 6250.52 mg/100 g, with mean value of 1853.03 mg/100 g while the crude protein content (CPC) being between 33.43 and 47.51%, with mean value of 40.81%. The highest number of accessions (45.97%) showed TAC between 1000~1900 mg/100 g while 30.96% of accessions showed CPC between 41~43%. Among the 746 accessions considered, 11 (IT142935, 175818, 175855, 177191, 177209, 177211, 177214, 177216, 177218, 177220, 177274) of them showed TAC above 4000 mg/100 g. C-3-O-G was found to be the major contributor to TAC showing strong correlation. Accessions with green cotyledon color showed high mean TAC compared to those having yellow cotyledon color, and accessions with dull seed lust color showed high mean TAC than those having shiny seed lust color. One hundred seeds weight and maturity date showed positive correlation with all anthocyanin contents, except for Pt-3-O-G in the latter case. The overall result of the present study could be used as background for developing new black soybean cultivars and breeds with high anthocyanin and protein contents. The result depicted that many of the accessions could be used as potential parental lines.

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주경 상부엽 제거시기가 콩의 절위별 Sink형질 변이에 미치는 영향 (Variation of Sink Components in Response to Removal Time of Upper Leaf on Main Stem in Soybean)

  • 박춘봉;이중호
    • 한국작물학회지
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    • 제40권1호
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    • pp.16-25
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    • 1995
  • 콩에 있어서 source인 상부엽의 제거시기에 따라 sink인 협이나 종실에 얼마 만큼 영향을 미치는가 를 절위별 협 및 종실의 변화를 중심으로 연구한 결과를 요약하면 다음과 같다. 1. 엽제거에 의해 주경의 협수감소가 시작되는 절위는 엽제거 시기가 늦을수록 낮아졌고, 개화 후 25일 엽재거구가 15일구보다 엽제거 시기가 10일 늦은데도 6∼8절위에서 감소폭이 커 개화후 25일이 결협에 중요한 시기인 것으로 나타났다. 2. 주경의 상부엽 제거로 주걍하부엽과 분지엽의 수광량이 증가하였는데도 엽무제거구보다 이들 부의의 물질 집적량이 적어 이 부위의 동화물질이 다른 부위로 전류된 양상을 보였다. 3. 협당입수는 개화후 35일 엽제거구가 25일구보다 적어 개화후 35일이 입의 퇴화에 더 큰 영향을 준 시기였다. 4. 가장 주요한 sink인 종실의 종피 구열립은 주경의 정단에서 9절위까지 엽무제거구가 25∼35% 절위였으나 엽제거구는 대부분 10%이하였다.

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콩.옥수수 육묘용 파종기 개발에 관한 연구 (A Study on the Development of the Seeder for Soybean and Corn)

  • 김동억;김현환;김종구;이공인;김성기;장유섭
    • Journal of Biosystems Engineering
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    • 제35권5호
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    • pp.330-335
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    • 2010
  • Soybean (Glycine max Merr.) and corn (Zea mays L.) transplanting has increased because soybean and corn crops cultivated by the direct seeding method were often damaged by wild birds. The purpose of this study is to develop a seeder to sow soybean (Glycine max Merr.) and corn (Zea mays L.) in a plug tray. In order to find out design factors for a metering device of the seeder, metering characteristics on metering hole size and roller speed were experimentally investigated. Soybean (cv. 'Daewon') and corn (cv. 'Mibaekchal') were used as a materials for testing the seeder in this experiment. The metering hole size of roller suitable for Daewonkong and Mibaekchal was determined. Daewonkong was suitable for hole diameter of 10 mm and hole depth of 5.5 mm, and Mibaekcal was suitable for hole diameter of 9 mm and hole depth of 5.5 mm. At a brush length of 4 mm, one grain seeding rates of Daewonkong and Mibaekchal was 99% and 93% respectively. By inducing Mibaekchal to the hole by swing, one grain seeding rate of that increased from 91.9% to 97.7%. When roller speed is 4 m per minut, seeding efficiency of prototype was 110 sheets per hour.

적엽 및 제협처리가 콩의 동화물질 전류에 미치는 영향 (Effects of Leaf and Pod Removal on Assimilate Translocation in Soybean Plants)

  • 성락춘;박지희
    • 한국작물학회지
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    • 제38권5호
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    • pp.377-382
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    • 1993
  • 콩의 생식생장기인 착협시(R3)에 상부40%와 하부 60%로 구분하여 엽제거와 협제거처리에 의한 엽과 종실의 건물중, 가용성 당, 전분, 단백질 및 기름함량에 미치는 영향을 구명하기 위하여 1992년 고려대학교 자연자원대학 덕소농장에 황금콩을 공시하여 실시한 시험결과를 요약하면 다음과 같다. 1. 상엽하협제거처리의 하부 엽 건물중이 가장 높았고 상부 종실 건물중은 가장 낮았다. 2. 가용성 당합량은 처리간 엽과 종실에서 차이가 없었다. 3. 전분함량은 상엽하협제거의 하부 여에서 가장 높았다. 4. 단백질함량은 하부 엽이 상부 엽보다 높았는데 종실에서는 하엽상협제거의 하부에서 가장 낮았다. 5. 기름합량은 하엽상협제거의 상부 엽과 하부 종실에서 가장 높았다. 6. 콩 생식생장기의 장거리 전류에 있어서 동화물질은 상부에서 하부로 이루어지며, 단백질원은 하부에서 상부로 일어나지만 엽에서의 재이동은 약했다.

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Non-destructive Method for Selection of Soybean Lines Contained High Protein and Oil by Near Infrared Reflectance Spectroscopy

  • Choung, Myoung-Gun;Baek, In-Youl;Kang, Sung-Taeg;Han, Won-Young;Shin, Doo-Chull;Moon, Huhn-Pal;Kang, Kwang-Hee
    • 한국작물학회지
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    • 제46권5호
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    • pp.401-406
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    • 2001
  • The applicability of non-destructive near infrared reflectance spectroscopic (NIRS) method was tested to determine the protein and oil contents of intact soybean [Glycine max (L.) Merr.] seeds. A total of 198 soybean calibration samples and 101 validation samples were used for NIRS equation development and validation, respectively. In the developed non-destructive NIRS equation for analysis of protein and oil contents, the most accurate equation was obtained at 2, 8, 6, 1(2nd derivative, 8 nm gap, 6 points smoothing, and 1 point second smoothing) and 2, 1, 20, 10 math treatment conditions with Standard Normal Variate and Detrend (SNVD) scatter correction method and entire spectrum (400-2500 nm) by using Modified Partial Least Squares (MPLS) regression, respectively. Validation of these non-destructive NIRS equations showed very low bias (protein: 0.060%, oil: -0.017%) and standard error of prediction (SEP, protein: 0.568 %, oil : 0.451 %) as well as high coefficient of determination ($R^2$, protein: 0.927, oil: 0.906). Therefore, these non-destructive NIRS equations can be applicable and reliable for determination of protein and oil content of intact soybean seeds, and non-destructive NIRS method could be used as a mass screening technique for selection of high protein and oil soybean in breeding programs.

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