• Journal of the Korean Society of Food Science and Nutrition
• /
• v.36 no.10
• /
• pp.1341-1350
• /
• 2007

This study was designed to ultimately develop a highly efficient mass production technology of bacterial cellulose isolated from the citrus gel fermented by G. hansenii TL-2C. Pilot equipment made with FRP vessel length (665 mm) ${\times}$ width (375 mm) ${\times}$ height (210 mm) was developed for mass production of the citrus gel. To develop the optimal conditions for mass production of citrus gel, comprised of citrus juice (6,000 mL) diluted 100 times, containing 5% seed bacteria, 10% sucrose, and 1% ethanol, citrus juice was fermented at $30^{\circ}C$ for 14 days, and gel productivity in pilot system was examined. BC was isolated and purified from the citrus gel, and their chemical composition and physicochemical properties were investigated.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.1
• /
• pp.170-175
• /
• 2004

This study was conducted to determine the minimum concentration of citrus juice for basal medium and also to search for an additional carbon source for the best production of the gel. A concentrate of citrus fruit juice of 65$^{\circ}$Brix, it was diluted to be used as a basal medium. Static cultivation of Gluconacetobacter hansenii TL-2C for 14 days at 3$0^{\circ}C$ produced the best gel with 7.5$\pm$0.4 mm thickness in the 6-fold diluted citrus Juice concentrate without any additional nutrient. However, the same thickness could be obtained with 60 to 100-fold diluted juice concentrate when refined white sugar was added at appropriate concentrations. Glucose was the most effective sugar for the both of gel and acid production, and optimal concentration of the sugar was 10$^{\circ}$Brix. Ethyl alcohol at 1.0% had synergistic effects in combination with refined sugar and increased the gel thickness up to 15.1 mm which was 1.85 times thicker than that of refined sugar alone. However, acetic acid was not effective. Gel productivity with supplement of ethanol was 172.6$\pm$8.4 g wet/L, and it was approximately equal to 4.7 g of dry gel/L.

• Journal of Radiation Industry
• /
• v.6 no.2
• /
• pp.159-164
• /
• 2012

Bacterial cellulose (BC) is generated from citrus gel by Gluconacetobacter hansenii TL-2C. BC has good properties such as high-burst pressure, high-water contact and the ultrafine highly nanofibrous structure of mimic natural extracellular matrix (ECM) for tissue engineering. In this study, acrylic acid (AAc) was grafted onto BC surfaces under aqueous conditions using gamma-ray irradiation, and then immobilized gelatin onto AAc-g-BC. The characterization of scaffolds was performed by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), toluidine blue O (TBO) assay. Morphology of gelatin and AAc incorporation onto BC nanofibers did not changed. Our study suggests that gelatin-immobilized BC nanofibers scaffold has a potentiality to fabricate 3D nanofibrous scaffolds for tissue engineering.