• Title/Summary/Keyword: Ginseng field

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Effect of P .E. film-mulching on the Damage to Tobacco Seedlings by the 'Dark Grey Cutworm', Agrotis tokionis Butler (P.E. 필름 피복(被覆)이 숫검은밤나방(Agrotis tokionis B.) 유충(幼蟲)에 의한 담배 피해(被害)에 미치는 영향(影響))

  • Kim, Sang-Seock;Boo, Kyung-Saeng;Hyun, Jai-Sun
    • Korean journal of applied entomology
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    • v.24 no.4 s.65
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    • pp.173-177
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    • 1986
  • This study was carried out to see what makes the 'dark grey cutworm', Agrotis tokionis Butler, inflict less damage in P.E. film-mulched tobacco fields than in nonmulched ones. In field plot experiment, the damage ratio of tobacco seed lings in mulched plots(m) was reduced by 63% compared with that in non mulched ones(n). The altered environments did not affect the cutworm in mortality, which was confirmed by recovery ratio, location of larvae in soil, and developmental age. But the m/n value and damage ratio in plots of different mulching methods strongly suggest that the P.E. film itself prevent larvae from cutting the plants. Larval mortality was rapidly increased between the end of July and the end of August.

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Developing a mass propagation technique for Aralia elata via somatic embryogenesis

  • Moon, H.K.;Lee, J.S.;Kim, T.S.
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2000.10b
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    • pp.16-17
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    • 2000
  • Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.

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Residue Levels of Organochlorine Pesticides on Paddy Field Soils in the Chungnam Area (충남지역(忠南地域) 논토양중 유기염소계(有機鹽素系) 농약(農藥)의 잔류수준(殘留水準))

  • Choi, Jong-Woo;Lee, Kyu-Seung;Lee, Jung-Kil;Noh, Kil-Bong
    • Korean Journal of Environmental Agriculture
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    • v.6 no.2
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    • pp.12-21
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    • 1987
  • Residues of some organo-chlorine pesticides on 108 solis collected from 5 sites of Chungnam avea during March 20-April 6, 1986 were investigated. 12 different kinds of Chemical components were detected showing the highest detection frequency with P.P'-DDE(67.6%) and the lowest with Heptachlor(1.8%). 4 different chemicals, ${\alpha}-BHC$, ${\gamma}-chlordane$, P.P'-DDD and P.P'-DDD and P.P'-DDE showed their average contents in the soils of 0.001 ppm and 0.005ppm for ${\beta}-BHC$, respectively. On the other hand, ${\alpha}-BHC$, ${\gamma}-chlordane$, P.P'-DDT. O.P'-DDT and P.P'-DDD showed 0.002ppm for their average contents of residues, respectively.

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Effect of Ginseng Radix Rubra Herbal-acupuncture Solution(GRR-HAS) on Gene Expression in SNU484 carcinomar cells (홍삼약침액(紅蔘藥鍼液)의 위암세포주(胃癌細胞柱) 유전자(遺傳子) 발현(發顯)에 미치는 영향(影響))

  • Won, Eun-Ju;Lee, Kyung-Min;Lee, Bong-Hyo;Lim, Seong-Chul;Jung, Tae-Young;Seo, Jung-Chul
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.39-47
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    • 2006
  • Objective : It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell lines. Oligonucleotide microarray approache was employed to screen the differential expression genes. Methods : GRR-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of GRR-HAS(0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip (Human genome Ul33 Plus 2.0., Affimatrix Co.). Results : It has no cytotoxic effects on both HepG2 and SNU484 cells in all concentrations(0.1, 0.5, 1.5, 10, 20mg/ml). In oligonucleotide microarray assay, in SNU484 cells, the number of more than twofold up-regulated genes was 346. The number of more than twofold down-regulated genes was 9. Discussion : This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.

A report of 17 unrecorded bacterial species of Korea belonging to the phylum Bacteroidetes

  • Cho, Jang-Cheon;Seong, Chi Nam;Joh, Kiseong;Cha, Chang-Jun;Bae, Jin-Woo;Yi, Hana;Lee, Soon Dong;Kim, Myung Kyum;Yoon, Jung-Hoon
    • Journal of Species Research
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    • v.7 no.3
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    • pp.231-239
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    • 2018
  • While screening indigenous prokaryotic species in Republic of Korea in 2017, a total of 17 bacterial strains assigned to the phylum Bacteroidetes were isolated from a variety of environmental habitats including water of fountain, tidal flat, plant root, soil, the gut of Russian grayling butterfly, ginseng field, seawater, lagoon and seashore sand. From the 16S rRNA gene sequence similarity of more than 98.7% and the formation of a robust phylogenetic clade with the closest species, it was found that the 17 strains belong to independent and recognized bacterial species. There has been no official report that the identified 17 species have been previously isolated in the Republic of Korea. Thus, 15 species in 10 genera of one family in the order Flavobacteriales, one species in one genus of one family in the order Cytophagales, and one species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidetes found in the Republic of Korea. Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.

Genetic Parameters for Some Leaf Characters in Flue-Cured Tobacco (Nicotiana tabacum L. ) at Two Representative Stalk Positions (황색종 연초(Nicotiana tabacum L.)의 엽위별 엽형질에 대한 유전자 효과분석)

  • 조수헌
    • Journal of the Korean Society of Tobacco Science
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    • v.10 no.2
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    • pp.117-122
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    • 1988
  • This experiment was conducted to study the nature of gene effects for the leaf characters in flue-cured tobacco. The genetic populations were derived from crosses between NC 2326 and Mc Nair 373, and NC 628 and DG-72. The generation means experiment Included the Pl, P2. Fl, F2, Bl and B2, which were frown at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute in 1984. Seedlings were transplanted to the field in a randomized block design with 3 replications. In each block, parental and Fl Plots contained 15 plants in a single row, F2, Bl and B2 plots being composed of 75 plant, in 5 rows. Leaf characters were measured of largest (middle leaf) and 5th leaf(top leaf) from the top after topping. Measurements of the length and width of leaf were obtained from the fresh the middle and top leaves, and weight of leaf, weight and width of midrib were from the satrap leaf after curing. Estimates of additive and dominance genetic variance were analyzed according to Gamble's biometrical model. The results obtained are as follows: 1. The additive gene effects were significant and larger than the dominance gene effects for all leaf and midrib characters in both stalk positions. 2. The dominance gene effects were significant for the length and width of leaf, and weight of midrib in the middle leaves. 3. The digenic epistatic effects were significant for the length and width of leaf in both stalk positions. 4. The additive gene effects were larger in the top than in the middle leaves and midrib characters.

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A study on the Gae-sung Han-ok of in the 『Mi-mang』 of novel (소설 『미망』 속 개성 한옥 고찰)

  • Kim, Bue-Dyel;Cho, Jeong-Sik
    • Journal of architectural history
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    • v.31 no.3
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    • pp.41-52
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    • 2022
  • This study explains the han-ok. in Gae-sung, which was antique and traditional city. In view of the fact that these were located in Gae-sung in North Korea hence direct investigate is not possible. Since there was no comprehensive study of Gaeseong han-ok, we have no choice but to rely on fragmentary records and memory of Gaeseong people. In accordance from the Japanese colonial era's surveys and sketches as well as how they were illustrated in the novel. Hark back to the peoples' thought of the han-ok years when they ran the survey in 1920s, the results were as follows: First, there are many houses so various classes in Mi-Mang. Jeon Cheo-man's house was a representative example of a rich family in Gae-sung, Jeon Je-seong(Jeon's 2nd son)'s house was located in Si-jeon(government licensed-markets) near by the South gate. In particular, Jeon Yi-seong(Jeon's 3rd son)'s house was in rural areas. He managed on immense ginseng field and worker's houses. Hae-ju's house was a small house off the track. Second, the outer house is low and the main house is high and neat. Lastly, it was confirmed that it corresponded to sketches recorded by architects, such as neat housekeeping, long stones used for various purposes, invisible flat gates, the appearance of old and old houses and the composition of each room.

A Study on Changes of Physico-Chemical Properties of Plow Layer Soil and its Response of Tobacco Growth under Poly Ethylene Film Mulching Condition. -1. Effect of Poly Ethylene Film Mulch on Changes of Soil Temperature and Soil Moisture Content in the Rhizosphere (연초피복재배(煙草被覆栽培)에서 작토시(作土尸)의 이화학성변화(理化學性變化)와 연초생장반응(煙草生長反應)에 관(關)한 연구(硏究) -1. 피복조건(被覆條件)이 근권(根圈)의 토양온도(土壤溫度)와 수분변화(水分變化)에 미치는 영향(影響))

  • Hong, Soon-Dal;Lee, Yun-Hwan;Kim, Jai-Joung;Cho, Seong-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.1
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    • pp.20-26
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    • 1985
  • Under the field condition mulched by polyethylene film, changes of the soil moisture content and soil temperature due to the meteorological influence, which have had a great effect upon productivity of upland - field crop, was investigated. The results obtained were as follows; 1. During the early growth stage from the first part of April to the last part of May, soil temperature at 15cm below the mulched surface of ridge (13:00) was about $5^{\circ}C$ higher than that of non-mulched plot. 20 days earlier than normal soil temperature reached up to $20^{\circ}C$ due to the mulching. The increasing of soil temperature resulted from poly ethylene film mulching had a similar tendency for the annual experiment of 3 years (1982-1984). 2. Changes of the soil moisture content in the plow layer during the growing season was remarkably less in the mulched plot than in the non-mulched plot due to suppression of evaporation by polyethylene film. 3. Soil moisture contents of the plots planted with tobacco was lower than those of the non-planted plots owing to the more transpiration induced by growing of the tobacco plant. As the results, it was refered that initial fresh weight of above ground part of tobacco that had an influence upon the consumption of soil moisture by the transpiration rather than evaporation was about 250g per plant. 4. At the appreciable amount of rainfall (for instance; 63.5mm), soil moisture content at middle place between plants in the plow layer of the mulched plot was not increased owing to the infiltration interruption by polyethylene film. 5. By the comparatively small amount of rainfall (e.g. 20mm) after the drought period, leaf water potential of the mulched plot was not increased as much as that of the non-mulched plot owing to the less moisture content of soil resulted from interruption of rainfall.

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Characteristics on the Field Growth of Plantlets Regenerated from Leaf Segment Cultures of Boxthorn (Lycium chinense Mill.) (구기자나무의 엽절편체에서 유도된 재분화식물체의 포장생육 특성)

  • Kim, Dong-Chan;Chung, Hae-Joon;Min, Byung-Hoon;Yang, Deok-Chun;Kim, Su-Dong;Lee, Bong-Chun
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.129-134
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    • 2002
  • 194 plantlets regenerated from leaf explants of boxthorn 'Cheonyang' were cultivated to investigate their morphological characteristics in the field for 2 years. Based on the morphology of leaves, 66.1% of them had elliptical type leaf, the same as that of mother plants, while 22.2% in oval type, 7.2% in obovate type, 2.6% in long-obvate type and 2.1% in lanceolate type. They were classified to 4 groups; group A was selected with both high fruit size and fruit yield, group B with only high fruit size, group C with larger or thicken leaf, and group D with multiple brenches. In comparision of production efficiency between the selected groups and mother plants, group A (99741, 99781, 99854, 99870 and 99886) were longer (2.1 to 2.7 mm) in length of fruits and higher in fruit production (15 to 30%) as compared to mother plants. Croup D (99797 and 99892) was higher in leaf production (7.2%) as compared to boxthorn 'CL1-48', which is the highest in leaf production among boxthorn veriaties.

Green Tea Root Is a Potential Natural Surfactant and Is Protective against the Detrimental Stimulant PM2.5 in Human Normal Epidermal Keratinocytes (녹차뿌리 특화 사포닌의 천연 계면 활성력을 이용한 새로운 안티폴루션 기작 연구)

  • Na, Hye-Won;Lee, Yeongran;Park, Jun Seong;Lee, Tae Ryoung;Kim, Hyoung-June
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.44 no.1
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    • pp.67-72
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    • 2018
  • Green tea (Camellia sinensis L.) has been widely explored for its medicinal applications. However, most of the studies had targeted the green tea leaf, while other parts remained unexplored. In this study, protective effect of green tea root extract on Normal Human Epidermal Keratinocytes (NHEKs) against the damage induced by an external stimulant (PM2.5) was confirmed. Thirty-year-old green tea root samples were collected from Amorepacific's Dolsongi tea field and green tea root extract was prepared with 70% ethanol. Total crude saponin content in green tea root extract was 54%, which is much higher than that in ginseng extract. Our results suggest that green tea root extract can be used as a natural surfactant in cosmetics. For evaluating its protective effect against the damage induced by PM2.5, IL-36G was used as a biomarker. IL-36G mRNA expression level increased remarkable upon PM2.5 treatment in NHEKs. Moreover, IL-36G was recently reported to be expressed in psoriasis lesions. Results showed significant decrease of IL-36G expression by treatment of green tea root extract. In conclusion, thirty-year-old green tea root extract can be used as a natural surfactant with a high saponin content and may have protective effect against the damage induced by PM2.5.