• 한국연초학회지
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• 제16권2호
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• pp.128-133
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• 1994

This study was carried out to get agronomic information about the tobacco culture in paddy soil where incidence of grey tobacco leaves used to appearly uptake of iron, manganese and chlorine due to soil acidity and high water level. Application of lime to paddy soil(pH5.4-5.6) reduced content of iron, manganese known as proceeding materials giving rise to variegated grey tobacco after curing, compared with non-treatment. Grey leaves were found mainly at lower and middle stalk positions, and incidence of grey tobacco was lowered by application of lime in the well drained field but was not affected by level of fertilizer application. Amendment of soil acidity by lime tended to decrease chlorine and manganese content in leaves. Nicotine and mangenese content of leaves were lowered by reduction of one-quarter fertilizer level. In case of lime treatment, increase of yield reached to 4-6% comparing with those of non-treatment but price per kg was not affected. Reduction of N fertilizer level to three-quarters had the equal yield but high quality of leaf comparing with standard fertilization in paddy field.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.88-94
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• 2005

Plant tissue culture studies have been done for the preservation of medicinal plant resources and efficient production of pharmaceutically important secondary metabolites. Micropropagation methods for Cephaelis ipecacuanha have been established and these methods enabled much more efficient propagation of the plants than the conventional methods using seedling or layering. The C. ipecacuanha plants derived from tissue culture grew uniformly in the field and they showed higher alkaloid contents compared to the plants grown from seedlings. Hairy root cultures of C. ipecacuanha and Panax ginseng have been established by infection with Agrobacterium rhizogenes, and the production of important pharmaceuticals by these cultures have been successfully demonstrated. In the case of C. ipecacuanha, the highest alkaloid yields from the hairy roots cultured for 8 weeks were 2.75-fold cephaeline (5.5 mg) and one third emetine (0.7 mg) compared with those from the roots of one-year old plant propagated through shoot-tip culture and cultivated in a greenhouse (2.0 mg cephaeline and 2.0 mg emetine). In the case of P. ginseng, ginsenoside contents in the hairy roots optimally cultured for 4 weeks were much higher than those in the roots of 4-year old field-grown plant. Thus our medicinal plant tissue cultures demonstrate desirable properties. However, they are always exposed to danger of microbial contamination or unexpected trouble of culture facilities. Cryopreservation of plant tissue cultures is a reliable method for long-term preservation. Cryopreservation studies on these cultures are also presented.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.913-918
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• 2007

A Gram-positive, aerobic, rod-shaped, nonmotile, endospore-forming bacterium, designated Gsoil $349^T$, was isolated from soil of a ginseng field and characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences revealed that the strain Gsoil $349^T$ belongs to the family Paenibacillaceae, and the sequence showed closest similarity with Cohnella thermotolerans DSM $17683^T$ (94.1%) and Cohnella hongkongensis DSM $17642^T$ (93.6%). The strain showed less than 91.3% 16S rRNA gene sequence similarity with Paenibacillus species. In addition, the presence of MK-7 as the major menaquinone and $anteiso-C_{15:0},\;iso-C_{16:0},\;and\;C_{16:0}$ as major fatty acids suggested its affiliation to the genus Cohnella. The G+C content of the genomic DNA was 53.4 mol%. On the basis of its phenotypic characteristics and phylogenetic distinctiveness, strain Gsoil $349^T$ should be treated as a novel species within the genus Cohnella for which the name Cohnella panacarvi sp. nov. is proposed. The type strain is Gsoil $349^T\;(=KCTC\;13060^T=\;DSM\;18696^T)$.

• Journal of Plant Biotechnology
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• 제30권2호
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• pp.167-172
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• 2003

Immature zygotic embryos of Eleutherococcus senticosus seeds matured rapidly within one month when the seeds comprising zygotic embryos were pieced to small size and cultured on 1/2 MS medium. Frequency of somatic embryos formation was declined rapidly when the zygotic embryos germinated and grew to plantlets. Embryogenic cells were induced by consecutive subculture of somatic embryos on MS medium with 1.0mg/L2,4-D. After heart-shaped somatic embryos were induced by suspension culture, these embryos were plated onto petri dish to support maturation of embryos. Germination of embryos occurred on medium with 5mg/L GA$_3$and transferred to culture bowl to stimulate the further growth. Frequency of soil survival of plantlets was influenced by soil mixture (perlite and peatmoss). The suitable combination of perlite and peatmoss was 1:5, and the soil survival rate was 78% after 4 months. The soil transferred plantlets were over-wintered in field condition after defoliation. New year sprouting of plants was achieved successfully and they grew to adult plants. These results indicate that the systematic procecure of plant production in E. senticosus for micro propagation.

• 한국연초학회지
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• 제11권1호
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• pp.3-9
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• 1989

Field experiment was conducted to find out the cause of calcium deficiency of burley tobacco (Nicotiana tabacum L.), Liming materials and application rate were CaSO4 : Ca 35kg/10a, CaCO3 : 35kg/10a and agricultural lime : Ca 142kg/10a. The breakdown in tissues at tips and margins of upper leaves was developed at maximum growth stage. If complete breakdown and death does not occur and growth takes place later, giving the leaf a scalloped appearance. Upper leaves contained lower calcium content than other leaves. The stem and midvein of calcium deficient plants contained lower calcium and calcium minus oxalic acid, but higher oxalic acid contents than those of normal plant. Fresh leaves of limed plot contained higher calcium and oxalic acid, but not significant increment of calcium minus oxalic acid than those of unlimed plot. Since calcium oxalate is insoluble, it could precipitate within the culls if the calcium and oxalic acid are accessible to each other. It suggest that high level of oxalic acid in stem and midvein could be interfering with translocation of calcium to upper leaves.

• Journal of Ginseng Research
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• 제9권2호
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• pp.154-162
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• 1985

We report a newassay method on the measurement of the catalase activity, whose utilzation value is considered to be remarkable in the field of plant biochemistry. We named this method as a De-Coupling method. The essence of de-coupling method is the separation between the enzyme reaction and the indicator reaction. The optimum condition of the enzyme reaction was found to be following: on addition of 1 ml of substrate (H2O2: 20mM) to the fixture of the crude extract of enzyme (volume: 0.2 ml) and the ammonium phosphate buffer (volume: 1.8 ml; 0.93 M phosphate, 1.6M NHB, 2.5 M methanol, pH 7.0). After 30, 60 and 90 seconds of the enzyme reactions are proceeded, the reactions are terminated by 25% of tai-chloro-acetate (final concentration of 5%), respectively. The precipitated materials by tai-chloro-acetate was removed by the centrifugation (2000g, 10minutes). Formaldehyde produced in the enzymatic reaction was reacted with 2ml of acetylacetone (60mM). The indicator reaction -(HANTSCH REAKT10N)- in which lutidine is formed, was proceeded for 60 minutes at $25^{\circ}C$.

• 한국연초학회지
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• 제8권2호
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• pp.9-17
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• 1986

Effect of nitrogen rate with and without chance of Phosphorus and Potassium rate was investigated in a field experiment. Percentage of plant mortality after transplanting increased with N rates above 32.5kg/10a. The content of total nitrogen increased and postassium decreased slightly as the rates of applied N was increased, but the contents of total alkaloid and phosphorus were not affected at each growing stage. It was considered that the application of 17.5kg of P2O5 and 35.0kg of K2O Per 10a might be sufficient for high yield and good Quality. As the N rates being increased, the yield, value, contents of total alkaloid and total nitrogen of cured leaf increased However, the Brice per kg was not significantly different among 17.5 ~ 37.5kg / 10a of N rates. The application of 37.5kg/10a of N may be profitable for farm economy; but, the N application should be controlled 17.5~22.5kg/10a for the low nitrogen and alkaloid leaf.

• 한국연초학회지
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• 제16권1호
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• pp.53-56
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• 1994

This study was aimed at finding out the effect of herbicide quinclorac (3, 7-dichloro-8- quinoline carbxylic acid) on tobacco culture in paddy soil. Herbicides quinclorac(Pozol, Pulta and Tomata) was randomly applied to subdivided paddy soil in Sep. 1992, and tobacco seedlings were transplanted to the field when seedling averaged 2cm in height in April 1993. Experiment plots were divided into non - treatment, standard(3kg/10a) and two times(6kg/10a) in amounts for each three herbicides. The symptoms of damage from herbicide were sighted when the plants had developed 8-9 leaves at 30 day after transplanting in standard amount plots, and when the plants had developed 4-5 leaves at 20 days after transplanting in two times treatments. The new leaves from damaged plants were bended out, and leaf color changed from green to dark - green, and then gradually advanced to abnormal narrow leaves. Standard treatments of herbicides showed a decrease of 18% in price per kg, 18% in yield and 33% in value per 10a than those of non - treatment, while two times treatments showed a decrease of 33%, 29%, 52% compared with non - treatment, respectively.

• 한국토양비료학회지
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• 제41권3호
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• pp.170-176
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• 2008

대학 부속농장의 밭 토양을 선정하여 토양 중 AMF 밀도 향상과 토양 물리성 개선을 위하여 기주작물로서 겨울작물인 보리와 호밀, 여름작물인 수단그라스와 콩을 재배하는 작부체계 유형별로 AMF 밀도 변화와 토양특성을 조사하였다. 재배작물의 생산량은 겨울작물 중 호밀을 재배한 포장 ($3,045kg\;10a^{-1}$)이 가장 높았으며, 호밀 재배 후 여름작물 중 수단그라스를 재배한 포장 ($2,757kg\;10a^{-1}$)과 보리를 재배한 후 콩을 재배한 포장 ($1,628kg\;10a^{-1}$) 순으로 건물 생산량을 보였다. 유기물 함량에서는 큰 변화는 인정되지 않았으나 입단화율은 보리를 재배한 포장이 45.7%, 호밀을 재배한 후 수단그라스를 재배한 포장에서 45.1%로 높아졌다. 포자밀도는 겨울작물을 재배한 포장이 전체적으로 증가하는 경향을 보였고, 여름작물은 수단그라스를 재배한 포장에서 건토 1 g당 64개로 콩을 재배한 포장보다 증가하였다. AMF 외생균사 길이는 겨울작물이 건토 1 g당 1.5~2.0 m 수준이었으며, 여름작물은 수단그라스를 재배한 포장이 건토 1 g당 2.6~2.9 m, 콩을 재배한 포장이 건토 1 g당 1.7~2.2 m 수준으로 대조구보다 높게 나타났다. 토양 중 Glomalin 함량은 작물을 재배한 포장이 대조구보다 높은 함량을 나타내었다. 그 중에서도 보리를 재배한 후 이어 콩을 재배한 포장에서 건토 1 g당 1.7 mg로 가장 높게 나타났다. 이상의 결과에서 인삼 재배 예정지의 토양관리를 위한 작부체계는 겨울작물의 보리를 재배한 다음 여름작물의 수단그라스를 재배하는 유형이 AMF 포자밀도 향상과 토양 물리성 개선에 효과적인 것으로 밝혀졌다.

• 한국약용작물학회지
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• 제15권3호
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• pp.157-161
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• 2007

인삼 연작장해 원인과 경감대책을 수립하고자 2004년에는 2005년까지 작물과학원 증평시험지 연작토양에서 인삼 수확경 과년수와 예정지관리 유무에 따른 시험을 수행한 결과를 요약하면 다음과 같다. 인삼 연작지 수확경과년수가 짧을수록 생존율이 낮아지며 3${\sim}$4년 경과구 81${\sim}$95%의 뿌리썩음 이병주율을 보였다. 예정지 관리구는 미관리구보다 생존율이 다소 높으며 뿌리 썩음병 피해도 예정지 미관리와 같은 경향으로 예정지 관리효과는 크지 않다. 수확경과년수가 10년이상의 연작지에서도 연작장해를 일으키는 병원균 (C. destructans)이 분리되므로 재작 또는 연작시는 이점을 고려하여 재배지를 재선정하거나 예정지 토양관리대책을 수립해야 할 것으로 판단된다.