• Journal of Ginseng Research
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• v.35 no.4
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• pp.421-428
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• 2011

This study evaluated cholineresterase inhibitory activity of Korean white ginseng extract (WGE), red ginseng extract (RGE), and black ginseng extract (BGE) and the cholinergic effect on scopolamine (SCOP)-induced amnesic mice. WGE, RGE, and BGE inhibited acetylcholineserase (AChE), as well as butyrylcholineserase (BuChE) in a concentration-dependent manner. BGE presented strong inhibition of AChE with an $IC_{50}$ value of 1.72 mg/mL, followed by WGE (5.89 mg/mL), RGE (6.30 mg/mL), respectively. The inhibitory activity of the three ginseng extracts on BuChE showed similar values among the groups. To better understand the mechanisms of the possible effect of ginseng extract on the cholinergic function, this study assessed the expression of the cholinergic markers of choline acetyltransferase (ChAT) and AChE using western blot and RT-PCR analysis in the brains of amnesic mice. Treatment with ginseng extracts led to inhibition of AChE expression and, the activation of ChAT expression in the hippocampus and the cerebral cortex of amnesic mice as induced by SCOP. The results suggest that ginseng extracts including BGE, appear to modulate the metabolism of acetylchoine (ACh), which would greatly increase synaptic ACh levels and most potently revert SCOP-induced amnesia.

• Proceedings of the Ginseng society Conference
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• 1988.08a
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• pp.43-46
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• 1988

The nephrotoxicity of dichromate was reduced upon the administration of red ginseng $S_I-fraction$. Rabbits treated with dichromate showed acute renal failure and the majority of animals died within ten days. Mortality and physioligical status were significantly improved by the administration of ginseng. The effect of red ginseng $S_I-fraction$ was studied by hematological analysis. urinalysis. and histophathological examination. It was found that red ginseng $S_I-fraction$ has a potent effect on glomerulotubular imbalance induced by dichromate. In addition, $S_I-fraction$ normalized the rate of glycogenolysis. glycolysis. and the rate of lactate production. which had been accelerated by intravenous dichromate injection. Red ginseng $S_I-fraction$ was superior to ascorbic acid. CaEDT A and furocemide in the protection and recorvery from dichromate-induced acute renal failure.

• Korean journal of food and cookery science
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• v.30 no.6
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• pp.679-686
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• 2014

This study was carried out to evaluate the quality characteristics of cookies added with various concentrations (0, 1, 3 and 5%) of ginseng leaf powder. The pH of the cookies significantly decreased with increased ginseng leaf powder concentrations (p<0.05), but the density of cookies showed no significant differences. Spread factor, loss rate, and leaving rate of cookies decreased according to the amount of added ginseng leaf powder. The lightness and yellowness of cookies decreased as the concentration of the ginseng leaf powder increased whereas no significant difference in the redness was found with increased ginseng leaf concentration. In the texture analysis, the hardness of the cookies increased according to the concentration of ginseng leaf. DPPH free radical scavenging activity of the cookies significantly increased with increased ginseng leaf concentration (p<0.05). In the sensory evaluation, sensory scores for color, taste and overall acceptability were highest in the 3% ginseng leaf cookies. Thus, our results suggested that the optimum amount of ginseng leaf powder to add to cookies was 3%.

• Korean Journal of Organic Agriculture
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• v.29 no.4
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• pp.439-457
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• 2021

Since ancient times, ginseng has been cultivated in Korea, and related cultures are also rich. However, the domestic ginseng industry is currently facing many crises. As a source of ginseng, Korea needs to come up with a strategy to cope with the crisis of the ginseng industry and implement it efficiently and effectively using limited resources. This study used the Analytic Hierarchy Process (AHP) to analyze the priority of policies so that ginseng policies can be effectively implemented. The survey was conducted by dividing into two groups of experts, 'policy managers' and 'field experts'. As a result of the study, policy managers seem to believe that the consumption of ginseng is important for the development of the ginseng industry. On the other hand, the field expert group believes that the government should take the initiative to increase the supply capacity of the ginseng industry. Through this study, it will be possible to help in determining the importance of policy, recognizing that the viewpoints of policy can be different for the ginseng industry to develop.

• Journal of Ginseng Research
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• v.19 no.2
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• pp.87-92
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• 1995

We conducted a prospective cohort study to evaluate the preventive effect of ginseng on the development of cancer in the population over 40 years old residing in Kangwha-eup from August 1987 to December 1992. Alnong 4, 634 persons (2, 362 men, 2, 272 women), 335 (7.6%) persons had died. Cancer accounted for 79 (22.8%) of the total death. Of 4, 634 persons eligible for analysis, 70.475 (3, 263) were ginseng intakes. Ginseng intakes had a decreased risk (RR=0.48, 95% Cl : 0.34~0.67) compared with non-intakes. The relative risk of cancer according to the kind of ginseng was 0.23 (95% Cl : 0.08~ 0.63) for fresh ginseng extract intakes. There was a decrease in risk with rising the frequency of ginseng intake, showing statistically significant dose-response relationship. The longer the duration of ginseng intake or the greater the total number of ginseng consumed, the lower the risk. Newly diagnosed cancer cases have been identified: 42 stomach, 24 lung, 14 liver and 57 at other sites. The relative risks of ginseng intakes were 0.34 (95% Cl 0.19~0.60) in gastric cancer and 0.27 (95% Cl : 0.12-0.60) in lung cancer. Among ginseng preparations, fresh ginseng intakes were significantly associated with the decreased risk of gastric cancer (RR: 0.19, 95% Cl : 0.04~0.98). These results strongly revealed that Panax Ginseng C.A. Meyer (Korean ginseng) has preventive effect against cancer.

• Korean Journal of Medicinal Crop Science
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• v.15 no.3
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• pp.220-226
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• 2007

For the analysis of hazard factors and the development of GAP (Good Agricultural Practices) Ginseng, 10 ginseng cultural farms wished certified GAP were selected at Geumsan-gun area, a representative site of ginseng cultivation in Korea. In order to verify the safety of GAP ginseng, possible contamination of pesticide and heavy metal residues, and microbial hazard were analyzed. Soil and water around ginseng cultivation field, and ginseng were investigated. Eighty-one pesticides including carbendazim were used as typical pesticide against plant pathogens and insect pests of ginseng plant and general crops. There was no excess the maximum residue limit (MRL) in residue figure of the soil. Including the residue figure of the arsenic (0.81 ml/kg) and 7 other heavy metals was also suitable to cultivate the ginseng plant. The irrigation water and dilution water for pesticide application were also safety level for GAP. Fresh ginsengs from the farms were sampled and investigated pesticide residues and contaminations of bacteria. Among 23 pesticides tested, we didn't detect any kinds of pesticide residues, but tolclofos-methyl was frequently found in the other ginseng field. On the investigation of microorganism hazards, 2 gram negative bacteria and 1 gram positive bacterium were found in the fresh ginseng. Number of total bacteria was $1.5{\times}10^3$ cfu/ml, which was less than the other agriculture products. At these results, 10 selected ginseng farms were good cultural places for GAP ginseng production and the ginseng cultured from Geumsan-gun area were a good safe far human.

• Journal of Ginseng Research
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• v.30 no.4
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• pp.212-219
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• 2006

Research literatures (e.g. research report, scientific paper and thesis, published between 2001 and 2005) of 332 collected from KISTI, KISS, ARPC databases were investigated and analyzed to give an overview of domestic ginseng researches. Most of recent ginseng researches have been performed by private nonprofit institutions and individuals, which suggests that a role of public institution fur ginseng researches needed to be enlarged. In terms of research fields, cultivation and agricultural researches have been mainly accomplished in ginseng research. Cell physiology and reproductive function researches occupied large part of pharmacological studies, but clinical studies were relatively limited. Studies on general food products development have taken the main part in fields of food products including functional foods. As the result of analysis of 312 domestic ginseng patents by classifications of world international property organization (WIPO), it was categorized into food technology, medicinal fermentation development technology, fermentation technology, and agriculture-fisheries technology. This integrated overview of the widely scattered ginseng literatures shows current general research trends in Korea and will be able to suggest further needs on cultivation, studies and industralization of ginseng.

• Mycobiology
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• v.42 no.2
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• pp.174-180
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• 2014

We analyzed the genetic diversity of Cylindrocarpon destructans isolates obtained from Korean ginseng (i.e., Panax ginseng) roots by performing virulence tests and nuclear ribosomal gene internal transcribed spacer (ITS) and mitochondrial small subunit (mt SSU) rDNA sequence analysis. The phylogenetic relationship analysis performed using ITS DNA sequences and isolates from other hosts helped confirm that all the Korean C. destructans isolates belonged to Nectria/Neonectria radicicola complex. The results of in vivo and ex vivo virulence tests showed that the C. destructans isolates could be divided into two groups according to their distinctive difference in virulence and the genetic diversity. The highly virulent Korean isolates in pathogenicity group II (PG II), together with foreign isolates from P. ginseng and P. quinquefolius, formed a single group. The weakly virulent isolates in pathogenicity group I, together with the foreign isolates from other host plants, formed another group and exhibited a greater genetic diversity than the isolates of PG II, as confirmed by the mt SSU rDNA sequence analysis. In addition, as the weakly virulent Korean isolates were genetically very similar to the foreign isolates from other hosts, they were likely to originate from hosts other than the ginseng plants.

• Journal of Microbiology and Biotechnology
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• v.28 no.6
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• pp.860-873
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• 2018

Although ginseng marc is a by-product obtained during manufacturing of various commercial ginseng products and has been routinely discarded as a waste, it still contains considerable amounts of potential bioactive compounds, including saponins and polysaccharides. Previously, we reported that ginseng oligosaccharides derived from ginseng marc polysaccharides by enzymatic hydrolysis exert immunostimulatory activities in macrophages and these activated macrophages are in turn able to inhibit the growth of skin melanoma cells by inducing apoptosis. In the present study, a more detailed investigation of the immunostimulatory activity and underlying action mechanisms of an enzymatic hydrolysate (GEH) containing these oligosaccharides derived from ginseng marc polysaccharides was performed. The levels of proinflammatory cytokines and anti-inflammatory cytokines were measured in GEH-stimulated RAW264.7 macrophages using RT-PCR analysis and ELISA. The expression levels of Toll-like receptor 2 (TLR2) and TLR4, Dectin-1, and MerTK were measured by RT-PCR analysis or western blot analysis, and the phagocytic activities of GEH-challenged bone marrow-derived macrophages toward apoptotic Jurkat cells were assayed using fluorescence microscopy. GEH induced the production of both proinflammatory cytokines $TNF-{\alpha}$ and IL-6, and anti-inflammatory cytokine IL-10 in RAW 264.7 cells. The expression of the TLR2 and MerTK mRNAs was increased upon GEH treatment. Phagocytosis of apoptotic Jurkat cells was enhanced in GEH-treated macrophages. Based on the results, this enzymatic hydrolysate (GEH) containing oligosaccharides exerts immunostimulatory effects by maintaining the balance between M1 and M2 cytokines, facilitating macrophage activation and contributing to the efficient phagocytosis of apoptotic cells. Therefore, the GEH could be developed as value-added, health-beneficial food materials with immunostimulatory effects.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.143-153
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• 2019

Background: Ginseng is one of the well-known medicinal plants, exhibiting diverse medicinal effects. Its roots possess anticancer and antiaging properties and are being used in the medical systems of East Asian countries. It is grown in low-light and low-temperature conditions, and its growth is strongly inhibited at temperatures above $25^{\circ}C$. However, the molecular responses of ginseng to heat stress are currently poorly understood, especially at the protein level. Methods: We used a shotgun proteomics approach to investigate the effect of heat stress on ginseng leaves. We monitored their photosynthetic efficiency to confirm physiological responses to a high-temperature stress. Results: The results showed a reduction in photosynthetic efficiency on heat treatment ($35^{\circ}C$) starting at 48 h. Label-free quantitative proteome analysis led to the identification of 3,332 proteins, of which 847 were differentially modulated in response to heat stress. The MapMan analysis showed that the proteins with increased abundance were mainly associated with antioxidant and translation-regulating activities, whereas the proteins related to the receptor and structural-binding activities exhibited decreased abundance. Several other proteins including chaperones, G-proteins, calcium-signaling proteins, transcription factors, and transfer/carrier proteins were specifically downregulated. Conclusion: These results increase our understanding of heat stress responses in the leaves of ginseng at the protein level, for the first time providing a resource for the scientific community.