• Journal of Life Science
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• v.20 no.8
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• pp.1268-1275
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• 2010

This study was carried out to investigate the anti-proliferative activity of solid-state fermented medicinal herbs which include Phellinus baumii. Methanol extracts were prepared from 36 different medicinal herbs and their fermented counterparts. These extracts were used to treat human colorectal HCT116 cell, human embryonic kidney cell HEK-293, pre-adipocyte cell 3T3-L1, and pre-osteoblast cell MC3T3-E1 for 24 hr. At a concentration of 100 ${\mu}g/ml$, the extracts of Amomum villosum, Cnidium officinale Makino, Dendrobium moniliforme, Dictamnus dasycarpus, Diospyros kaki Thunb, Eucommia ulmoides Oliv, Ginkgo biloba L, Magnolia denudata Desrousseaux, Orostachys japonicus, Panax notoginseng, Pharbitis nil Choisy, Polygala tenuifolia and Trichosanthes kirilowii (seed) led to a < 50% decrease in cell proliferation, and mycelium of P. baumii showed a 46.3% decrease in cell proliferation. Meanwhile, the extracts of the 25 fermented herbs showed similar anti-proliferative activities compared to those of individual non-fermented herbs. However, the extracts of the fermented Drynaria fortunei Kunze (1), Lycium chinense Mill (2), Fritillaria thunbergii Miquel (3) and Prunus persica showed increased anti-proliferative activity. The $IC_{50}s$ of (1), (2) and (3) were especially decreased to 28, 85 and 80 ${\mu}g/ml$ from 394, 917 and 149 ${\mu}g/ml$, respectively. Furthermore, the cytotoxicity of the extracts of fermented (1), (2) and (3) against HEK-293, 3T3-L1, and MC3T3-E1was negligible up to 200 ${\mu}g/ml$. These results suggest that solid-state fermentation using the mycellium of P. baumiiproduce potential anti-cancer agents or strengthen the bioactivity of medicinal herbs.

• Journal of Forest and Environmental Science
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• v.19 no.1
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• pp.85-98
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• 2003

The articles, published in the Journal of Korean Forestry from Number 1(1962) to Number 6, Volume 91(2002), were surveyed and investigated for the research trend analysis about resource-plants for special use or purpose, i. e., edible plants, medicinal plants, feed resource, landscape plants, fiber plants, industrial usuage, and bee plants. If the purpose or subject matter of the research was construction or furniture timber production, mushrooms and/or pulp and paper, such research was not included in this study. These articles were classified again depending on the content of research into 14 categories: habitat environment, ecology, physiology, propagation, silviculture (tending and culture), genetics and breeding, identification, insect and disease control, animal-related research, component analysis, vegetation survey, biotechnology, management, and review. Among the total 1.434 articles published, 396 ones (27.6%) were related with plants for special use or purpose. Vegetation survey was 60 (15.2%): physiology 56(14.1%) : genetics and breeding 56(14.1%): propagation 53(13.4%): and ecology 37(9.3%). Siviculture research field included 11 articles (2.8%), which indicates that the management of resource-plants is so far from economic income as seen in the low number of management research filed articles, i. e., only 6 reports (1.5%) Korean white pine was most popular for research and included 42 articles: Robinia pseudoacacia 23: Castanea crenata 14: and ginkgo tree 14. Research related with these species had focused mainly on propagation, physiology, genetics and breeding, ecology and pest control. Based on this survey and analysis, the followings are suggested: 1. More research is required on forest herbaceous plants. 2. Cooperative research work with other industrial and/or scientific area is recommendable for commercialization including medicine, cosmetics, and food etc. 3. Research on resource-plant conservation, which includes biology, social education and policy, should be supported for next generation. 4. Mutual correspondence and information exchange about the research results between researchers and institutes is more necessary than now.

• Radiation Oncology Journal
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• v.20 no.2
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• pp.147-154
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• 2002

Purpose : Gingko biloba extract (GBE), a natural product extracted from Gingko leaves, is known to increase the radiosensitivity of tumors. This radiosensitization probably arises from the increase in the peripheral blood flow by decreasing the blood viscosity and relaxing the vasospasm. The influence of a GBE on the metabolic status in fibrosarcoma II (FSall) of a C3H mouse was investigated using $^{31}P$ magnetic resonance spectroscopy (MRS). Materials and Methods : Eighteen C3H mice with fibrosarcoma II $(from\;100\;mm^3\;to\;130\;mm^3)$ were prepared for this experiment. The mice were divided into 2 groups; one (9 mice) without a priming dose, and the other (9 mice) with a priming dose of GBE. The GBE priming dose (100 mg/kg) was administered by an intraperitoneal (i.p.) injection 24 hours prior to the measurement. First $^{31}P$ MRS spectra were measured in the mice from each group as a baseline and test dose of GBE (100 mg/kg) was then administered to each group. One hour later, the $^{31}P$ MRS spectra were measured again to evaluate the change in the energy metabolic status. Results : In the group without the priming dose, the mean pH, PCr/Pi, PME/ATP, Pi/ATP, PCr/(Pi+PME) values 1 hour after the test dose were not changed significantly compared to the values at the baseline. However, in the group with the priming dose, the mean PCr/Pi, Pi/ATP, PCr/(Pi+PME) values 1 hour after the test dose changed from the baseline values of 0.49, 0.77, 0.17 to 0.74, 0.57, 0.28 respectively. According to the paired t-test, the differences were statistically significant. Conclusion : The above findings suggest that the metabolic status is significantly improved after administering GBE if the priming dose is given 24 hours earlier. This shows that the radiosensitizing effect of GBE is based on the increase of tumor blood flow and the improvement in the metabolic status.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.145-152
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• 1998

We conducted this study as a fundamental study on the response of various tree species against acid rain. The tree species used for this study were Zelkova serrata, Robinia pseudoacacia, Quercus acutissima, Prunus serrulata, Ginkgo biloba, Pinus koraiensis and Pinus densiflora. The leaves were examined for the pH changes by treatment time and the chlorophyll content into various pH solution in vitro. The results obtained were as follows ; 1. When the leaves were immersed in the solution of various pH(pH 3.0-pH 6.0) levels, the pH were changed to species specific pH ranges as pH 5.0~pH 5.5 of Z. serrata, pH 5.5~pH 6.0 of R. pseudoacacia, pH 4.5~pH 5.0 of Q. acutissima, pH 5.5~pH 6.0 of P. serrulata, pH 3.5~pH 4.5 of G. biloba, pH 3.5~pH 4.5 of P. koraiensis until 48 hours. However, in case of P. densiflora, it was difficult to find specific pH range of the species. Z. serrata, R. pseudoacacia and P. serrulata showed a little pH increasing by pH 2.0 solution treatment, while other species showed no change by the solution. 2. The amount of chlorophyll contents in Z. serrata, R. pseudoacacia and P. serrulata were decreased after immersing in the pH 2.0 solution. Chlorophyll content was almost constant in other pH levels. Other species showed almost constant chlorophyll contents in various pH levels and treatment time.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.28 no.1
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• pp.12-26
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• 2010

This study was conducted to investigate current states and management of the protected trees in Gangwon-do. There were 701 protected trees in 42 species designated by 2008, which were distributed at 425 places. Major species were Zelkova serrata(29%), Pinus densiflora(24%), Ulmus davidiana var. japonica(11%), and Ginkgo biloba(5%). The protected trees played various roles in the villages, mostly as sacred trees(43%). The 51% of protected trees were 300 years or more in age, the 53% were more than 20 m in height, and the 57% were more than 300 cm in girth at breast height. The 69% of the protected trees have been designated for protection in 1982 and the rest have been designated by the local cities by 2008. The species number of the protected trees in Youngseo region was greater than that of the Youngdong region. The major species were Zelkova serrata (33%) and Pinus densiflora(17%) in Youngseo region, and were Pinus densiflora (38%) and Zelkova serrata(19%) in Youngdong region. The ratio of native species to exotic species was 33:1 in Youngseo region, and were 25:6 in Youngdong region. The 29% of protected trees were located in the field, 28% in the mountain, and 25% in the villages. The 45% of the ground were covered by herbaceous plants, 39% exposed, and 10% gravel mulch. Most of the protected trees had good growing conditions. The 70% of the protected trees were under good maintenance, and the rest were under undesirable conditions. The soil characteristics of the protected tree areas in Gangwon-do were not much different from those of Korean average. It seemed to be necessary to increase designation of protected trees based on the tradition, history, local value and academic application in Gangwon-do. In addition, it is desirable to suggest plans to utilize and manage protected tree areas for enhancing the value of natural heritages in Gangwon-do.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.4
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• pp.62-74
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• 2015

This study was carried out to provide examples of planting methods for the construction of street green spaces with abundant greenery by analyzing road type, surrounding land use and planting structures in street green spaces on Port Island, Kobe, Japan. Port Island_(total area: 826ha) is a marine cultural city located in Japan's first artificial island with facilities of urban function and port facilities. The study site was designated at 11 plots of $55{\sim}285m^2$ on Port Island, and topography structure styles were divided into four types with mounding style, slope style, slope and flat style, flat style according to the adjacent roads width. The area adjacent to the middle roads with high levels of noise and pollution set up the mounding style, slope style, slope and flat style of multi-layer structures using topographic properties. The area adjacent to small roads focused on a green strip with shrubs on a flat style. Surrounding land-uses include a public institution, housing complex, and a commercial building. The planting concept was a buffer and landspace function in case of the middle road_(lane 4) while the small road_(lane 2) was a landspace function. Planting species were diverse with Liquidambe formosana, Cinnamomum camphora, Sapium sebiferum, Cedrela sinensis, Laeocarpus sylvestris var. ellipticus, Ginkgo biloba, Prunus serrulata var. spontanea, Zelkova serrata, Quercus glauca, Juniperus chinensis, Magnolia kobus, Rhododendron spp., Camellia japonica, Abelia mosanensis, etc. Planting density was 0.02~0.08(0.04) individual/$m^2$ at the canopy layer, 0.02~0.08(0.04) individual/$m^2$ at the understory layer. Ratio of green coverage was 40.0~173.7(93.0)% at the canopy layer, 2.1~79.8(34.9)% at the understory layer and 17.9~64.2(32.9)% at the shrub layer. $Gr{\ddot{u}}volumenzahl$ was $1.43{\sim}6.67(4.13)m^3/m^2$ at the canopy layer, $0.02{\sim}2.01(0.85)m^3/m^2$ at the understory layer and $0.14{\sim}0.58(0.26)m^3/m^2$ at the shrub layer. The ratio of green coverage of street green space on Port Island was higher than that of Seoul, and particularly, the ratio of green coverage and $gr{\ddot{u}}volumenzahl$ at the shrub layer differed, compared to the main street green space in Korea. The result of this study may be applicable to other coastal reclaimed cities in terms of setting methods for street greenery considering the topography structure, planting structure and planting function.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.4
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• pp.37-49
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• 2015

The goal of this research is to examine air temperature changes according to tree type, plantation type, roadside green area structure, and green volume of street green area within a city. The plantation type that could be analyzed for comparison by tree type with over 3 species was 1 rows of tree+shrubs. The results of analysis of average air temperature difference between pedestrian and car streets vis-a-vis 1 row of tree+shrub in high air temperature areas were: Pinus densiflora, $1.35^{\circ}C$; Zelkova serrata, $1.84^{\circ}C$; Ginkgo biloba, $2.00^{\circ}C$; Platanus occidentalis, $2.57^{\circ}C$. This standard large wide canopy species was analyzed by the roadside to provide shade to have a significant impact on air temperature reduction. In terms of analysis of the relationship between plantation type of roadside trees and air temperature, the average air temperature difference for 1 row of tree type was $1.80^{\circ}C$; for 2 rows of trees it was $2.15^{\circ}C$. In terms of analysis of the relationship between the roadside green area structure and air temperature, for tree type, average air temperature $1.94^{\circ}C$: for tree+shrub type, average air temperature $2.49^{\circ}C$; for tree+mid-size tree+shrub type, average air temperature $2.57^{\circ}C$. That is, air temperature reduction was more effective in a multi-layer structure than a single layer structure. In the relationship analysis of green volume and air temperature reduction, the air temperature reduction effect was enlarged as there was a large amount of green volume. There was a relationship with the green volume of the road, the size of the tree and number of tree layers and a multi-layer structured form of planting. The canopy volume was large and there were a great number of rows of the tree layer and the plantation type of multi-layer structure, which is what is meant through a relationship with the green volume along the roadside. Green composition standards for air temperature reduction effects and functional improvement were proposed based on the result. For a pedestrian street width of 3m or less in the field being ideal, deciduous broadleaf trees in which the canopy volume is small and the structure of the tree+shrub type through the greatest 1m green bend were proposed. For a pedestrian street width of over 3m, deciduous broadleaf trees in which the canopy volume is large and is multi-layer planted with green bend over 1m, tree+mid-size tree+shrub type was proposed.

• Journal of the Korean Institute of Landscape Architecture
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• v.15 no.3
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• pp.33-50
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• 1988

This study was executed to find out how to improve on the planting and use of the plants in Kangweon- Do by surveying the planting areas -gardens, parks, streets, schools, etc. - in five cities of this province, and to select available native plants by surveying the main mountains in this province. The results are as follows ; 1. The species number within surrey areas was 319 species. and the species of which planting frequency was very high were Hibiscus syriacus, Juniperus chinensis, Buxus microphylla v. koreana, etc.. 2. The species number of school trees and flowers of 202 schools in Kangweon-Do were 33 species, 32 species respectively. and the species of school trees and flowers that showed the highest preference were Juniperus chinensis, Forsythia koreana each. 3. The species number of flowers and trees designated in 22 City and Keun were 14 species, 7 species respectively, and the species of flowers and trees that presented the highest designation frequency were Rhododendron schlippenbachii, Ginkgo biloba each. 4. The street trees planted along the main streets in Kangweon-Do were 18 species and 84,939 individuals, and the ratio of Populus alba${\times}$glandulosa was the highest among occurrence individuals. 5, As for the composition ratios of life forms of plants within survey areas, the ratio of deciduous broad-leaved tree was the highest as about 56% and that of deciduous coniferous tree was the lowest as about 1.6%. The ratios of native species versus exotic were 43 : 57 6. Through these results, it was thought that the diversification of planing species, the selection of plants suitable to each space and the generalization of use of native species were needed. So 254 plants native to Kangweon-Do were presented to correspond to these requirements.

• Research in Plant Disease
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• v.18 no.1
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• pp.1-9
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• 2012

A total of 20 agricultural organic materials including inorganic compounds, plant oils and plant extracts were used in the study for assessing the control efficacy on pepper diseases. Among inorganic compounds, only copper hydroxide showed inhibitory effect on both Phytophthora capsici causing Phytophthora blight and Colletotrichum acutatum causing anthracnose. Phosphorous acid inhibited the growth of P. capsici on PDA, and Sulfur/quicklime had it on that of C. acutatum. Plant essential oil, rosemary oil, and rapeseed oil among plant oils and plant extract of Japanese apricot/ginkgo nut inhibited the mycelial growth of the two pathogens. In the screening using pepper plant seedlings, the control efficacy on Phytophthora blight in 6-leaf stage of seedling was superior to that in 4-leaf stage of seedling. A protective effect on Phytophthora blight was displayed by copper hydroxide, sulfur/quicklime, water soluble calcium, phosphorous acid, plant essential oil, and cloves extract. When C. acutatum was inoculated by the non-wound method, copper hydroxide and rapeseed oil showed excellent protective activities with control values of 91.3% and 82.6%, respectively. However, copper hydroxide did not show any activity, when C. acutatum was inoculated after wounding pepper fruits. All organic materials never showed the curative effect on Phytophthora blight and anthracnose in pepper seedling assay and fruit assay.

• Journal of Korean Society of Forest Science
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• v.7 no.1
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• pp.8-18
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• 1968

This experiment is a comparative study of influence on the seedling in individual pH treatment of culture soil I. T-R ratio and growth 1. T-R ratio has almost similar tendency in its range according to tree species, but in individual treatment there were lots of differences. In the strong acidic and the strong alkali conditions T-R ratio was small. This means the seedling body is out of balance. 2. Where the resistance to H-ion was stronger, the growth of root-system, that is, the resistance of fine root was comparatively better. 3. The growth of root system was greatly influenced by pH, and in the condition of pH3, pH8, pH9, the growth was especially worse. 4. Ginkgo biloba, Pinus rigida and Pinus banksiana kept on growing in the strong acidic condition. 5. The growth season has higher pH of culture soil than the dormant season, and tree species which have strong resistance of acidic had better growth and had stronger resistance of draught in the acidic condition of culture soil ; especially, Pinus rigida and Pinus banksiana were worse in the alkali condition. II Seedling pH 1. According to the tree species, there were differences in seedling pH, and tree species, considred to be strong in resistance of draught, had been high in seedling pH. 2. Seedling pH of the growth season was higher than that of the dormant season. 3. Seedling pH was not significant in pH treatment of the culture soil.