• 동아시아식생활학회지
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• 제26권3호
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• pp.207-214
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• 2016

This study investigated the effects of ginger (Zingiber officinale Roscoe) on antioxidant and antiproliferative activities in A2058 human melanoma cells. The antioxidant and antiproliferative activities of 70% ethanol extracts of Zingiber officinale Roscoe were identified based on DPPH and ABTS free radical scavenging capacities. Treatment of cells with Zingiber officinale Roscoe at concentrations of 0, 0.2, and 0.4 mg/mL for 24 hours significantly reduced cell viability as determined by Hoechst 33258 nuclear staining, apoptosis analysis, and Western blotting analysis, respectively. In our study, 70% ethanol extracts of Zingiber officinale Roscoe exhibited antioxidant activity and inhibited A2058 cell growth in a dose-dependent manner. Concomitant activation of the mitochondria-dependent apoptotic pathway of A2058 human melanoma cells by Zingiber officinale Roscoe extracts was mediated via modulation of Bax and Bcl-2 expression, which activated cleavage of caspases-3, caspases-9, and poly ADP-ribose polymerase. The findings of study indicate that Zingiber officinale Roscoe extracts induce apoptosis in A2058 human melanoma cells, and this phenomenon occurs via the death receptor-mediated and intrinsic pathways.

• Plant Resources
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• 제8권1호
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• pp.44-47
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• 2005

Activated carbon(AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on growth and yield of Ginger(Zingiber officinale) as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated carbon added with $5-10\%$, suggesting that optimum amount of activated carbon was ranged from 5 to $15\%$. Growth and enlargement of the root were improved by $10\%$ AC with higher rhizome length and weight.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4597-4606
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• 2012

Cholangiocarcinoma (CCA) is an uncommon adenocarcinoma which arises from the epithelial cells of the bile ducts. The aim of the study was to investigate the cytotoxicity, toxicity, and anticancer activity of a crude ethanolic extract of ginger (Zingiber officinale Roscoe) against CCA. Cytotoxic activity against a CCA cell line (CL-6) was assessed by calcein-AM and Hoechst 33342 assays and anti-oxidant activity was evaluated using the DPPH assay. Investigation of apoptotic activity was performed by DNA fragmentation assay and induction of genes that may be involved in the resistance of CCA to anticancer drugs (MDR1, MRP1, MRP2, and MRP3) was examined by real-time PCR. To investigate anti-CCA activity in vivo, a total of 80 OV and nitrosamine (OV/DMN)-induced CCA hamsters were fed with the ginger extract at doses of 1000, 3000, and 5000 mg/kg body weight daily or every alternate day for 30 days. Control groups consisting of 10 hamsters for each group were fed with 5-fluorouracil (positive control) or distilled water (untreated control). Median $IC_{50}$ (concentration that inhibits cell growth by 50%) values for cytotoxicity and anti-oxidant activities of the crude ethanolic extract of ginger were 10.95, 53.15, and $27.86{\mu}g/ml$, respectively. More than ten DNA fragments were visualized and up to 7-9 fold up-regulation of MDR1 and MRP3 genes was observed following exposure to the ethanolic extract of ginger. Acute and subacute toxicity tests indicated absence of any significant toxicity at the maximum dose of 5,000 mg/kg body weight given by intragastric gavage. The survival time and survival rate of the CCA-bearing hamsters were significantly prolonged compared to the control group (median of 54 vs 17 weeks). Results from these in vitro and in vivo studies thus indicate promising anticancer activity of the crude ethanolic extract of ginger against CCA with the absence of any significant toxicity. Moreover, MDR1 and MRP3 may be involved in conferring resistance of CCA to the ginger extract.

• 한국응용곤충학회지
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• 제56권2호
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• pp.171-177
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• 2017

식물기생성 선충인 뿌리혹선충과 뿌리썩이선충은 국내에서 생강을 포함한 수입 구근류에서 주로 검출되는 검역대상 해충이다. 그러나 이러한 선충류가 검출된 수입 생강의 경우 적절한 소독처리기준이 마련되어 있지 않아 폐기 및 반송처리로 인한 경제적 손실이 발생하고 있다. 본 연구에서는 생강에 침입한 검역 대상 선충의 사멸을 위한 식물소독처리 기준 마련을 위해 뿌리혹선충과 뿌리썩이선충을 사멸할 수 있는 온탕침지법에 관하여 조사하였다. 그 결과, 뿌리혹선충과 뿌리썩이선충은 각각 $48^{\circ}C$와 $49^{\circ}C$에서 30초간의 온탕침지 처리로 사멸되었다. $52.5^{\circ}C$로 설정된 60 L의 항온수조에 침지된 생강의 열전도 조사에서 생강 중심부와 내부 5 mm 두께의 온도가 $50^{\circ}C$까지 도달하기까지는 각각 10~32분과 6~16분이 소요되었으며 $51^{\circ}C$에서 30분 동안 온탕침지한 생강은 정상적으로 생육하였다. 본 결과를 바탕으로 뿌리혹선충의 유충을 생강에 인공접종 한 후 $51^{\circ}C$에서 30분간 온탕침지 하였을 때 처리한 선충이 모두 사멸되었다. 따라서 이상의 온탕침지 처리 조건은 생강에 영향을 주지 않고 두 종의 선충을 사멸시킬 수 있는 식물소독법의 기초자료가 될 것이다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.149.3-150
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• 2003

[6]-Gingerol, a major pungent ingredient of ginger (Zingiber officinale Roscoe, Zingiberaceae) has a wide array of pharmacologic effects. Our previous studies have demonstrated that [6]-gingerol inhibits mouse skin tumor promotion and anchorage-independent growth of cultured mouse epidermal cells stimulated with epidermal growth factor. In this study, we have investigated the molecular mechanisms underlying anti-tumor promoting effects of ［6］-gingerol on mouse skin carcinogenesis. (omitted)

• 생약학회지
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• 제48권1호
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• pp.25-30
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• 2017

This study was performed to evaluate the lifespan extending effects of the dried ginger (Zingiber officnale Roscoe, Zingiberaceae). Ethanol extract of the dried ginger was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions, and lifespan-extending effects of the fractions were investigated using Caenorhabditis elegans model system. Ethyl acetate-soluble fraction showed the most potent longevity property. Furthermore, ethyl acetate-soluble fraction elevated thermal stress tolerance, and increased expression of stress resistance protein. Further studies were performed to investigate several aging-related factors such as reproduction, food intake, growth and movement of C. elegans. The results revealed that there were no significant changes in aging-related factors including reproduction, food intake and growth, however, ethyl acetate-soluble fraction treatment led to an up-regulation of locomotory ability of aged worms, suggesting ethyl acetate-soluble fraction affected healthspan as well as lifespan of nematode.

• 한국자원식물학회지
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• 제12권3호
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• pp.209-214
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• 1999

비닐하우스에서 생강재배시 종강의 크기와 재식거리가 생강의 생육 및 수량에 미치는 영향을 조사하였다. 비닐하우스 내의 기온은 노지에 비하여 평균 9$^{\circ}C$ 높았으며, 지온은 평균 2-4$^{\circ}C$ 높았다. 하우스 재배는 노지재배에 비하여 초장, 엽면적, 경수, 괴경 수량이 크게 증가하였다. 하우스에서의 재식거리 30$\times$30cm에서 근경수량은 노지보다 121-183%의 증수 효과가 있었다. 60$\times$30cm의 재식거리구에서는 재식거리 30$\times$30cm구에 비하여 감소하였으나 노지재배에 비하여 76-82%증수되었다. 단위면적당 근경수량은 재식 밀도가 30$\times$30cm이고, 종구의 크기가 40g 일 때(4,631kg/10a)가장 많았다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 춘계학술대회 논문집
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• pp.95.1-95
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• 2003

Ginger (Zingiber officinale Roscoe, Zingiberaceae) has a wide array of pharmacologic effects. Our previous studies have demonstrated that [6]-gingerol, a major pungent ingredient of ginger, inhibits mouse skin tumor promotion and anchorage-independent growth of cultured mouse epidermal cells stimulated with epidermal growth factor. In this study, we have investigated the molecular mechanisms underlying chemopreventive effects of [6]-gingerol on mouse skin carcinogenesis. Cyclooxygenase-2 (COX-2), a key enzyme in the formation of prostaglandins, has been recognized as a molecular target of many chemopreventive as well as anti-inflammatory agents. The murine COX-2 promoter contains several transcriptional elements, particularly those involved in regulating inflammatory processes. One of the essential transcription factors responsible for COX-2 induction is NF-kappa B. Topical application of [6]-gingerol inhibited the COX-2 expression through suppression of NF-kappa B activation in phorbol ester-treated mouse skin. [6]-Gingerol, through down-regulation of p38 MAPK, abrogated the DNA binding activity of NF-kappa B by blocking phosphorylation of p65/RelA at the Ser 536 residue. These findings suggest that [6]-gingerol exerts an anti-tumor promotional activity through inhibition of the p38 MAPK-NF-kappa B siganling cascade in mouse skin.

• 한국식물병리학회지
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• 제13권3호
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• pp.152-159
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• 1997

병든 생강으로부터 분리한 6개 Pythium 균주들은 여러 가지 형태적, 생리적 특성에서 Pythium myriotylum Drechsler로 동정되었다. 이 균들은 생강 지하부의 싹, 근경, 뿌리, 땅가줄기 뿐만 아니라 지상부 잎, 줄기 전부분에 걸쳐 병원성이 강하였다. 이 균들은 오이, 수박 등 10개 작물 혹은 잡초의 어린 묘를 부패시켰으며 보리, 옥수수 등 공시한 3개 작물 유묘의 지상부 생장이나 뿌리 생장을 현저히 억제하였다. 이 균들의 생육 최고, 최적, 최저온도는 각각 39~45$^{\circ}C$, 33~37$^{\circ}C$, 5~7$^{\circ}C$였으며 생육최적 pH는 6~7이었다. 이 균들의 균사생장은 V-8 juice 배지에서 가장 빨랐으나 기중균사량은 PDA와 옥수수전즙배지에서 가장 많았다. 유주자낭과 장난기는 V-8 juice 배지에서 가장 잘 형성되었으며 유주자낭 형성 최적온도는 균주에 따라 20~35$^{\circ}C$, 장난기 형성은 15$^{\circ}C$의 저온에서 가장 양호하였다

• 농업과학연구
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• 제25권2호
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• pp.154-159
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• 1998

본 시험은 시비량의 차이가 생강의 생육 및 수량에 미치는 영향을 구명하기 위하여 수행한 시험 결과를 요약하면 다음과 같다. 1. 초장은 감량구에서 45.3cm, 표준구에서 52.6cm 증량구에서 49.4cm로 표준구가 가장 크게 나타났다. 2. 엽면적과 경수, 경직경은 증량구가 표준구에 비하여 높게 나타났으며, 지상부와 근경의 건물중도 증량구에서 가장 높게 나타났다. 3. 경수, 경직경, 엽면적, 지상부 건물중, 근경의 생체중 등이 비료 수준과의 관계에서 감량구와 표준구간, 감량구와 증량구간에 유의성이 인정되었다. 4. 수량은 감량구에서 1653kg/10a, 표준구에서 2248kg/10a, 증량구에서 2428kg/10a로 증량구가 표준구에 비하여 7.4%의 증수 효과를 나타냈다.