• Journal of the East Asian Society of Dietary Life
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• v.26 no.3
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• pp.207-214
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• 2016

This study investigated the effects of ginger (Zingiber officinale Roscoe) on antioxidant and antiproliferative activities in A2058 human melanoma cells. The antioxidant and antiproliferative activities of 70% ethanol extracts of Zingiber officinale Roscoe were identified based on DPPH and ABTS free radical scavenging capacities. Treatment of cells with Zingiber officinale Roscoe at concentrations of 0, 0.2, and 0.4 mg/mL for 24 hours significantly reduced cell viability as determined by Hoechst 33258 nuclear staining, apoptosis analysis, and Western blotting analysis, respectively. In our study, 70% ethanol extracts of Zingiber officinale Roscoe exhibited antioxidant activity and inhibited A2058 cell growth in a dose-dependent manner. Concomitant activation of the mitochondria-dependent apoptotic pathway of A2058 human melanoma cells by Zingiber officinale Roscoe extracts was mediated via modulation of Bax and Bcl-2 expression, which activated cleavage of caspases-3, caspases-9, and poly ADP-ribose polymerase. The findings of study indicate that Zingiber officinale Roscoe extracts induce apoptosis in A2058 human melanoma cells, and this phenomenon occurs via the death receptor-mediated and intrinsic pathways.

• Plant Resources
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• v.8 no.1
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• pp.44-47
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• 2005

Activated carbon(AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on growth and yield of Ginger(Zingiber officinale) as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated carbon added with $5-10\%$, suggesting that optimum amount of activated carbon was ranged from 5 to $15\%$. Growth and enlargement of the root were improved by $10\%$ AC with higher rhizome length and weight.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4597-4606
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• 2012

Cholangiocarcinoma (CCA) is an uncommon adenocarcinoma which arises from the epithelial cells of the bile ducts. The aim of the study was to investigate the cytotoxicity, toxicity, and anticancer activity of a crude ethanolic extract of ginger (Zingiber officinale Roscoe) against CCA. Cytotoxic activity against a CCA cell line (CL-6) was assessed by calcein-AM and Hoechst 33342 assays and anti-oxidant activity was evaluated using the DPPH assay. Investigation of apoptotic activity was performed by DNA fragmentation assay and induction of genes that may be involved in the resistance of CCA to anticancer drugs (MDR1, MRP1, MRP2, and MRP3) was examined by real-time PCR. To investigate anti-CCA activity in vivo, a total of 80 OV and nitrosamine (OV/DMN)-induced CCA hamsters were fed with the ginger extract at doses of 1000, 3000, and 5000 mg/kg body weight daily or every alternate day for 30 days. Control groups consisting of 10 hamsters for each group were fed with 5-fluorouracil (positive control) or distilled water (untreated control). Median $IC_{50}$ (concentration that inhibits cell growth by 50%) values for cytotoxicity and anti-oxidant activities of the crude ethanolic extract of ginger were 10.95, 53.15, and $27.86{\mu}g/ml$, respectively. More than ten DNA fragments were visualized and up to 7-9 fold up-regulation of MDR1 and MRP3 genes was observed following exposure to the ethanolic extract of ginger. Acute and subacute toxicity tests indicated absence of any significant toxicity at the maximum dose of 5,000 mg/kg body weight given by intragastric gavage. The survival time and survival rate of the CCA-bearing hamsters were significantly prolonged compared to the control group (median of 54 vs 17 weeks). Results from these in vitro and in vivo studies thus indicate promising anticancer activity of the crude ethanolic extract of ginger against CCA with the absence of any significant toxicity. Moreover, MDR1 and MRP3 may be involved in conferring resistance of CCA to the ginger extract.

• Korean journal of applied entomology
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• v.56 no.2
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• pp.171-177
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• 2017

Plant parasitic nematodes, Meloidogyne and Pratylenchus spp., are mostly detected in imported bulbs and tubers including a ginger, Zingiber officinale in Korea by quarantine inspection. However, there is little information on hot water immersion treatment (HWIT) for control of exotic nematodes, which induce economic loss by discard or send back to exporter, in imported gingers. In here, we determined that mortality of two plant parasitic nematodes and thermal stability of ginger. Meloidogyne and Pratylenchus spp. were completely killed at $48^{\circ}C$ and $49^{\circ}C$ for 30 sec by HWIT. Thermal conduction of Z. officinale to reach a target temperature as $50^{\circ}C$ take 10~32 min and 6~16 min for core and inner 5 mm region from surface, respectively. When ginger exposed at $51^{\circ}C$ for 30 min, growth of Z. officinale was not affected by heat treatment compared with control. Based on these results, HWIT at $51^{\circ}C$ for 30 min completely killed artificially infected juveniles of Meloidogyne spp. in Z. officinale. Therefore, this condition for HWIT will be used as fundamental information on phytosanitory to kill two plant parasitic nematodes without damage on ginger.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.149.3-150
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• 2003

[6]-Gingerol, a major pungent ingredient of ginger (Zingiber officinale Roscoe, Zingiberaceae) has a wide array of pharmacologic effects. Our previous studies have demonstrated that [6]-gingerol inhibits mouse skin tumor promotion and anchorage-independent growth of cultured mouse epidermal cells stimulated with epidermal growth factor. In this study, we have investigated the molecular mechanisms underlying anti-tumor promoting effects of ［6］-gingerol on mouse skin carcinogenesis. (omitted)

• Korean Journal of Pharmacognosy
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• v.48 no.1
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• pp.25-30
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• 2017

This study was performed to evaluate the lifespan extending effects of the dried ginger (Zingiber officnale Roscoe, Zingiberaceae). Ethanol extract of the dried ginger was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions, and lifespan-extending effects of the fractions were investigated using Caenorhabditis elegans model system. Ethyl acetate-soluble fraction showed the most potent longevity property. Furthermore, ethyl acetate-soluble fraction elevated thermal stress tolerance, and increased expression of stress resistance protein. Further studies were performed to investigate several aging-related factors such as reproduction, food intake, growth and movement of C. elegans. The results revealed that there were no significant changes in aging-related factors including reproduction, food intake and growth, however, ethyl acetate-soluble fraction treatment led to an up-regulation of locomotory ability of aged worms, suggesting ethyl acetate-soluble fraction affected healthspan as well as lifespan of nematode.

• Korean Journal of Plant Resources
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• v.12 no.3
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• pp.209-214
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• 1999

This experiment was carried out to investigate the influence of rhizome size and planting space on some agronomic characters and rhizome yield of ginger in greenhouse cultivation. The average air temperature was 9$^{\circ}C$ higher in greenhouse cultivation than in field. The average soil temperature also was 2-4$^{\circ}C$ higher in greenhouse. Plant height, leaf area, stem number and rhizome yield were significantly increased when cultivated at greenhouse compared to field. Under greenhouse the yield of rhizome in 30$\times$30cm planting space were appeared to be increase 121-183% compared with field cultivation. At the planting space of 60$\times$30cm, harvests were increased 76-82% comparing field cultivation. The most suitable rhizome size for planting at 30$\times$30cm planting space found to be 40g in greenhouse cultivation.

• Proceedings of the Korean Society of Toxicology Conference
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• 2003.05a
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• pp.95.1-95
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• 2003

Ginger (Zingiber officinale Roscoe, Zingiberaceae) has a wide array of pharmacologic effects. Our previous studies have demonstrated that [6]-gingerol, a major pungent ingredient of ginger, inhibits mouse skin tumor promotion and anchorage-independent growth of cultured mouse epidermal cells stimulated with epidermal growth factor. In this study, we have investigated the molecular mechanisms underlying chemopreventive effects of [6]-gingerol on mouse skin carcinogenesis. Cyclooxygenase-2 (COX-2), a key enzyme in the formation of prostaglandins, has been recognized as a molecular target of many chemopreventive as well as anti-inflammatory agents. The murine COX-2 promoter contains several transcriptional elements, particularly those involved in regulating inflammatory processes. One of the essential transcription factors responsible for COX-2 induction is NF-kappa B. Topical application of [6]-gingerol inhibited the COX-2 expression through suppression of NF-kappa B activation in phorbol ester-treated mouse skin. [6]-Gingerol, through down-regulation of p38 MAPK, abrogated the DNA binding activity of NF-kappa B by blocking phosphorylation of p65/RelA at the Ser 536 residue. These findings suggest that [6]-gingerol exerts an anti-tumor promotional activity through inhibition of the p38 MAPK-NF-kappa B siganling cascade in mouse skin.

• Korean Journal Plant Pathology
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• v.13 no.3
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• pp.152-159
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• 1997

Six pathogenic Pythium isolates obtained from diseased ginger rhizomes were identified as Pythum myriotyrum Drechsler based on various morphological and physiological characteristics. The isolates showed strong virulence on underground parts of buds, crowns, rhizomes, roots and aerial parts of leaves and stems as well. The isolates caused rot of germinated seeds of 10 different crops tested, including cucumber and pepper, and markedly inhibited seedling growth of 3 crops tested, including corn and barley. Maximum, optimum and minimum growth temperatures for P. myriotylum were 39~45$^{\circ}C$, 33~37$^{\circ}C$ and 5~7$^{\circ}C$, respectively. Optimum pH for the growth was 6~7. Mycelial linear growth was most rapid on V-8 juice agar, but aerial mycelia were most abundant on PDA and corn meal agar. Zoosporangial and oogonial formation was greatest on V-8 juice agar. Optimum temperatures for the production of zoosporangia and oogonia were 20~35$^{\circ}C$ and 15$^{\circ}C$, respectively.

• Korean Journal of Agricultural Science
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• v.25 no.2
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• pp.154-159
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• 1998

This experiment was carried out to define the effects of application amounts of the chemical fertilizers on plant growth and yield of the ginger. The results obtained are summarized as follows. Plant height of the ginger was 45.3 cm at lower level of fertilizer, 52.6 cm at recommended level, and 49.4 cm at higher level. Leaf area, stem number, and stem diameter were more significantly increased at higher level of fertilization compared to recommended rate. Dry weight of shoots and rhizomes were significantly increased with the increase of fertilization level. Stem number, stem diameter, leaf area, dry weight of shoots, and fresh weight of rhizomes were positively correlated with the amounts of fertilizer applied. Yield of rhizomes were 1, 653 kg at lower level of fertilizer, 2,248 kg at recommended level and 2,428 kg at higher level, respectively. Ginger yield showed 7.4% higher at higher level of fertilization compared to recommended rate.