• Journal of Applied Biological Chemistry
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• v.44 no.2
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• pp.53-58
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• 2001

Glycosidase activities in the grape flesh (Marguerite) were assayed, and the order of activity was marked as follows: ${\alpha}$-D-galactosidase>${\alpha}$-D-mannosidase>${\alpha}$-D-glucosidase>${\beta}$-D-galactosidase>${\beta}$-D-glucosidase. Of these glycosidases, ${\alpha}$- and ${\beta}$-D-galactosidases were prominently expressed by the treatment of gibberellic acid, resulting in 56 and 238% increase of activity, respectively. Most of ${\alpha}$-D-galactosidase was found in the flesh texture, and the activity increase by gibberellic acid occurred mostly in this tissue. The increase in ${\alpha}$-D-galactosidase activity was dependent on the concentration of gibberellic acid treated, showing a positive correlation. Gibberellic acid affected the content of total protein in the grape flesh, 49% increase by 75 ppm treatment. Above this concentration, higher gibberellic acid level did not influence the protein expression. Specific activity of the ${\alpha}$-D-galactosidase still increased, showing 24% increase in activity. Grape flesh subjected by gibberellic acid (100 ppm) resulted in the increased activity against a natural substrate, stachyose, showing 55% increase in activity from the grapes treated with 100 ppm of gibberellic acid. Other natural substrates, such as melibiose and raffinose, were also considerably hydrolyzed, and the extent was similar to that of stachyose hydrolysis. During postharvest storage, ${\alpha}$-D-galactosidase activity in the grape flesh increased by 51% after 20 days and then declined slowly.

• Microbiology and Biotechnology Letters
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• v.23 no.2
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• pp.119-122
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• 1995

A different form from Gibberella fujikuroi was isolated from the paddy field of Naju area. The strain, designated as Y107, was identified as Gibberrella sp. based on its morphological, physiological, and biochemical characteristics. The highest production of Gibberellic acid by the strain was achieved in a fermentation medium containing corn starch, glucose, soybean oil, soybean meal, NH$_{4}$NO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$, and trace elements.

• Microbiology and Biotechnology Letters
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• v.28 no.3
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• pp.152-155
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• 2000

Cultural Conditions for the Improvement in Gibberellic Acid Productivity by a Mutant of Gibberellafujikuroi ATCC 12616-Gibberella fujikuroi G-36 . Dh, Young-Jun. Department of Food and Biotechnolog}'r Dongshm Umversity, Naju 520-714, Korea - A mutant Gibberella jujih/roi G- 36 was selected by metagenesis of G/bberella fitjikuroi ATCC 12616 with mutagens such as N-methy1-N'~nitro~N"nitrosoguanidine and hydroxylamine for improving productivity of gibberellic acid. The mutant strain produced gibberellic acid (70 mg/l) more than that of wilde type. A fermentation medium containing glucose, $NH_4N0_3$, $MgS0_4$, $KH_2P0_4$ and trace elements was deve]oped for the maximal production of a gibberellic acid by the mutanL The Guctuating cultural temperature that was vaded from 300e to 20DC resulted in higher GA yield than that of fixed cu1tura] temperature at $28^{\circ}C$.

• Journal of Plant Biology
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• v.34 no.2
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• pp.159-163
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• 1991

The effect of dimethipin, one of the synthesized plant growth regulators, on the gibberellic acid-induced a-amylase activity in the barley de-embryonated seed system was investigated in order to elucidate the possible action mechanism of dimethipin. Dimethipin markedly inhibited the increase of mRNA and protein content, and a-amylase activity induced by gibberellic acid. The inhibitory effects were gradually decreased as the time interval between gibberellic acid treatment and dimethipin addition was made larger. Dimethipin inhibited the increase of mRNA content when added within 18 h from gibberellic acid treatment; however, it inhibited the increase of soluble protein content and a-amylase activity even added after 18 h from the treatment. These results suggest the possibility that dimethipin inhibit both mRNA synthesis and a-amylase protein synthesis.thesis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.1
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• pp.20-24
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• 1980

Seven different rice cultivars treated with different levels of gibberellic acid (0, 10, 50 and 100 PPM GA3) at panicle initiation stage. showed retardation effect on the panicle length and the 2nd internode from the top, where as, the total culm lengths were remarkably elongated, about 50-120% in short varieties and about 10-15% in the tall varieties over the control, mainly, because of the lower internodes elongation. The 2nd internode of Gamadi variety from Nepal was almost entirely shortened with GA3 application.

• Journal of Plant Biology
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• v.31 no.4
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• pp.249-258
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• 1988

Changes in starch content and activities of ADPG- and UDPG-starch synthase and $\alpha$- and, $\beta$-amylase were studied in order to investigate effects of gibberellic aicd and abscisic acid on endogenous starch content during shoot differentiation and protocorm propagation in Cymbidium spp. (Jungfrau) protocorm. Shoot differentiation was promoted during the degradation of endogenous starch and protocorn propagation was promoted during starch accumulation in protocorm. The activities of ADPG- and, UDPG-starch synthase and $\alpha$- and $\beta$-amylase seemed to be related with starch content. Shoot differentiation and protocorm propagation were slightly inhibited in protocorm explants treated with 100$\mu$M gibberellic acid. The explants treated with 10$\mu$M abscisic acid lost the capacity for shoot differentiation and protocorm propagation, and that could not be overcome by 100$\mu$M gibberellic acid added to culture medium. Starch content fluctuated as the control even after 10$\mu$M abscisic acid. None the less, the treatment completely inhibited shoot differentiation and protocorm propagation.

• Korean Journal of Environmental Biology
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• v.27 no.4
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• pp.406-413
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• 2009

The enzyme invertase contributes to sugar unloading, pathogen defense, differentiation and development in plants. We cloned the complete cDNA of a soluble acid invertase from pea seedlings (Pisum sativum) via RT-PCR and the rapid amplification of the cDNA end (RACE) technique. The full-length cDNA of the soluble pea invertase comprised 2237 bp and contained a complete open reading frame encoding 647 amino acids. The deduced amino acid sequence showed high homology to soluble acid invertases from various plants. Northern blot analysis demonstrated the soluble acid invertase gene of P. sativum was strongly expressed in sink organs such as shoot tips and root tips, and induced by abscisic acid, gibberellic acid and jasmonic acid in shoots. Especially, gibberellic acid enhanced the gene expression of the soluble acid invertase in a time-dependent manner. This study presents that the gene expression patterns of a soluble acid invertase from pea are strongly consistent with the suggestion that individual invertase gene product has different functions in the growing plant.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.4
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• pp.300-304
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• 2004

Gibberellic acid did not affect ethylene production, whereas gibberellin biosynthesis inhibitors triggered ethylene production in dormant tubers. Gibberellic acid did not induce sprouting of dormant tubers, however, treatment of gibberellin biosynthesis retardants enhanced sprouting rates. Sprouting rate in ancymidol-treated tubers was highest among gibberellin biosynthesis retardants. Sprouting rate of tubers treated with ancymidol increased to $91.4\%$. Batatasin-III content in $GA_3$ treated tuber was increased in the highest concentration $(30{\mu}g\;I^{-1})$. Tubers treated with mepiquat chloride, Batatasin-I was increased steadily, but contents of Batatasin-III and V showed dramatic decrease at the $ 1,000{\mu}g\;I^{-1})$ concentration. This infers that gibberellin biosynthesis retardants play key roles in promoting breaking dormancy on dormant tubers of Chinese yam.

• Korean Journal of Microbiology
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• v.7 no.4
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• pp.143-152
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• 1969

This investigation was proceeded to define the effects of gibberellic acid on the growth of Chlorella by determining the contents of chlorphyll and changes in various components in Chlorella cells according to the concentration of treated GA. The growth of Chlorella was accelerated with telative low concentrations of GA(10, 40 ppm) and was restrained with relative high concentrations of GA(70, 100, 200 ppm). The synthetic ability of chlorophyll of GA was inhibited generally in proportion to the concentration of treated-GA and the higher the concentration of GA was applied, the longer time was required in the restoration. The contents of RNA, protein and soluble carbohydrate were increased PCA-soluble amino acid and polysacharide were decreased in those cell components between the accelerated and restrained group. Consequently, the effect on accelerated growth in relative low concentrations of GA is considered to be caused by the powerful effet on expansion growth of GA. It is presumed that the effect of restrained growth in relative high concentrations of GA is due to the inhibitory effect on the chlorophyll synthesis.

• The Korean Journal of Mycology
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• v.19 no.2
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• pp.128-135
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• 1991

Diethyl ether soluble toxin produced by H. sativum had the characteristics of helmin­thosporal as based on UV, GC-MS, and chemical analysis, but was not a helminthosporaI. It was speculated that it was a polymer of helminthosporal. It stimulated the productions of reducing sugar in the barly endosperm like gibberellic acid, but acted in the responses on the barley roots and coleoptiles like gibberellic acid. It seemed to be involved in Foliar and Root rot diseases with host specificity, based on the analysis of linear regression.