• Journal of Applied Biological Chemistry
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• 제44권2호
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• pp.53-58
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• 2001

Glycosidase activities in the grape flesh (Marguerite) were assayed, and the order of activity was marked as follows: ${\alpha}$-D-galactosidase>${\alpha}$-D-mannosidase>${\alpha}$-D-glucosidase>${\beta}$-D-galactosidase>${\beta}$-D-glucosidase. Of these glycosidases, ${\alpha}$- and ${\beta}$-D-galactosidases were prominently expressed by the treatment of gibberellic acid, resulting in 56 and 238% increase of activity, respectively. Most of ${\alpha}$-D-galactosidase was found in the flesh texture, and the activity increase by gibberellic acid occurred mostly in this tissue. The increase in ${\alpha}$-D-galactosidase activity was dependent on the concentration of gibberellic acid treated, showing a positive correlation. Gibberellic acid affected the content of total protein in the grape flesh, 49% increase by 75 ppm treatment. Above this concentration, higher gibberellic acid level did not influence the protein expression. Specific activity of the ${\alpha}$-D-galactosidase still increased, showing 24% increase in activity. Grape flesh subjected by gibberellic acid (100 ppm) resulted in the increased activity against a natural substrate, stachyose, showing 55% increase in activity from the grapes treated with 100 ppm of gibberellic acid. Other natural substrates, such as melibiose and raffinose, were also considerably hydrolyzed, and the extent was similar to that of stachyose hydrolysis. During postharvest storage, ${\alpha}$-D-galactosidase activity in the grape flesh increased by 51% after 20 days and then declined slowly.

• 한국미생물·생명공학회지
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• 제23권2호
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• pp.119-122
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• 1995

A different form from Gibberella fujikuroi was isolated from the paddy field of Naju area. The strain, designated as Y107, was identified as Gibberrella sp. based on its morphological, physiological, and biochemical characteristics. The highest production of Gibberellic acid by the strain was achieved in a fermentation medium containing corn starch, glucose, soybean oil, soybean meal, NH$_{4}$NO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$, and trace elements.

• 한국미생물·생명공학회지
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• 제28권3호
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• pp.152-155
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• 2000

곰팡이 Gibberella fujikuroi ATCC12616을 mutagen N-methyl-N-nitro-N-nitrosoguanidine과 hydroxylamine으로 처리하여 지베렐린 생산수률이 가장 높은 변이주 G. fujikuroi G-26을 선별하였다 변이주 G.fujikuroi G-36는 모균주 G.fujikuroi ATCC 12616 과 비교하여 볼 때 지베렐린 생산수율이 약 32% 향상된 것으로 관찰 되었다 이 균주를 액체배지에서 발효시키면 주 탄소원으로 sucrose를 사용할 때 높은 생산성을 나타내었으며 초기배지 pH와 온도는 각각 pH 4.0$ 28^{\circ}C$조건이 가장 좋았다 배양온도를 $30^{\circ}C$에서 3일 배양후 $20^{\circ}C$로 낮추어 7일간 배양하면 130mg/l의 가장 높은 지베렐린이 생성됨을 알았다.

• Journal of Plant Biology
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• 제34권2호
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• pp.159-163
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• 1991

합성 식물생장조절제인 dimethipin의 작용기작을 밝히고자 보리 무배부 종자 시스템에서 gibberellic acid(GA3)에 의해 유도되는 ${\alpha}-amylase$ 활성에 미치는 dimethipin의 영향을 조사하였다. Dimethipin은 mRNA와 단백질 함량 및 GA3에 의해 유도되는 ${\alpha}-amylase$ 활성을 뚜렷하게 억제하였다. 그러나 억제효과는 GA3 처리 후 dimethipin 첨가시간 간격이 커질수록 점점 감소하였다. Dimethipin을 GA3 처리후 18시간 이내에 첨가하였을 때 mRNA 함량증가를 억제하였다: 그러나 GA3 처리 18시간 경과 후에 첨가하였을 경우에도 수용성 단백질 함량 및 ${\alpha}-amylase$ 활성 증가를 억제하였다. 이러한 결과로 미루어보아 dimethipin은 mRNA 합성과 ${\alpha}-amylase$ 단백질 합성을 공히 억제하는 것으로 사료된다.

• 한국작물학회지
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• 제25권1호
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• pp.20-24
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• 1980

Seven different rice cultivars treated with different levels of gibberellic acid (0, 10, 50 and 100 PPM GA3) at panicle initiation stage. showed retardation effect on the panicle length and the 2nd internode from the top, where as, the total culm lengths were remarkably elongated, about 50-120% in short varieties and about 10-15% in the tall varieties over the control, mainly, because of the lower internodes elongation. The 2nd internode of Gamadi variety from Nepal was almost entirely shortened with GA3 application.

• Journal of Plant Biology
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• 제31권4호
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• pp.249-258
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• 1988

Changes in starch content and activities of ADPG- and UDPG-starch synthase and $\alpha$- and, $\beta$-amylase were studied in order to investigate effects of gibberellic aicd and abscisic acid on endogenous starch content during shoot differentiation and protocorm propagation in Cymbidium spp. (Jungfrau) protocorm. Shoot differentiation was promoted during the degradation of endogenous starch and protocorn propagation was promoted during starch accumulation in protocorm. The activities of ADPG- and, UDPG-starch synthase and $\alpha$- and $\beta$-amylase seemed to be related with starch content. Shoot differentiation and protocorm propagation were slightly inhibited in protocorm explants treated with 100$\mu$M gibberellic acid. The explants treated with 10$\mu$M abscisic acid lost the capacity for shoot differentiation and protocorm propagation, and that could not be overcome by 100$\mu$M gibberellic acid added to culture medium. Starch content fluctuated as the control even after 10$\mu$M abscisic acid. None the less, the treatment completely inhibited shoot differentiation and protocorm propagation.

• 환경생물
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• 제27권4호
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• pp.406-413
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• 2009

The enzyme invertase contributes to sugar unloading, pathogen defense, differentiation and development in plants. We cloned the complete cDNA of a soluble acid invertase from pea seedlings (Pisum sativum) via RT-PCR and the rapid amplification of the cDNA end (RACE) technique. The full-length cDNA of the soluble pea invertase comprised 2237 bp and contained a complete open reading frame encoding 647 amino acids. The deduced amino acid sequence showed high homology to soluble acid invertases from various plants. Northern blot analysis demonstrated the soluble acid invertase gene of P. sativum was strongly expressed in sink organs such as shoot tips and root tips, and induced by abscisic acid, gibberellic acid and jasmonic acid in shoots. Especially, gibberellic acid enhanced the gene expression of the soluble acid invertase in a time-dependent manner. This study presents that the gene expression patterns of a soluble acid invertase from pea are strongly consistent with the suggestion that individual invertase gene product has different functions in the growing plant.

• 한국작물학회지
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• 제49권4호
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• pp.300-304
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• 2004

Gibberellic acid did not affect ethylene production, whereas gibberellin biosynthesis inhibitors triggered ethylene production in dormant tubers. Gibberellic acid did not induce sprouting of dormant tubers, however, treatment of gibberellin biosynthesis retardants enhanced sprouting rates. Sprouting rate in ancymidol-treated tubers was highest among gibberellin biosynthesis retardants. Sprouting rate of tubers treated with ancymidol increased to $91.4\%$. Batatasin-III content in $GA_3$ treated tuber was increased in the highest concentration $(30{\mu}g\;I^{-1})$. Tubers treated with mepiquat chloride, Batatasin-I was increased steadily, but contents of Batatasin-III and V showed dramatic decrease at the $ 1,000{\mu}g\;I^{-1})$ concentration. This infers that gibberellin biosynthesis retardants play key roles in promoting breaking dormancy on dormant tubers of Chinese yam.

• 미생물학회지
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• 제7권4호
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• pp.143-152
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• 1969

This investigation was proceeded to define the effects of gibberellic acid on the growth of Chlorella by determining the contents of chlorphyll and changes in various components in Chlorella cells according to the concentration of treated GA. The growth of Chlorella was accelerated with telative low concentrations of GA(10, 40 ppm) and was restrained with relative high concentrations of GA(70, 100, 200 ppm). The synthetic ability of chlorophyll of GA was inhibited generally in proportion to the concentration of treated-GA and the higher the concentration of GA was applied, the longer time was required in the restoration. The contents of RNA, protein and soluble carbohydrate were increased PCA-soluble amino acid and polysacharide were decreased in those cell components between the accelerated and restrained group. Consequently, the effect on accelerated growth in relative low concentrations of GA is considered to be caused by the powerful effet on expansion growth of GA. It is presumed that the effect of restrained growth in relative high concentrations of GA is due to the inhibitory effect on the chlorophyll synthesis.

• 한국균학회지
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• 제19권2호
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• pp.128-135
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• 1991

H. sativum에서 분리되고 명명된 D-toxin은 UV, GC-MS, 및 화학분석결과helminthosporal와 비슷한 결과를 얻었으나, helminthosporal와 같지는 않았다. 이 식물독물질은 barley endosperm실험에서 gibbe-rellic acid와 같은 결과를 반응을 나타내었으나, 보리뿌리 및 줄기반응에서 gibberellic acid와 같지가 않았다. 또한, 이 물질은 통계처리결과 은 반응결과를 Host specific disease로 알려진, Foliar spot and Root rot diseases에 작용하는 것으로 나타났다.