• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.328-333
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• 1995

Leaf quality and fatty acid compositions of collected perilla related genus and species germplasms such as Perilla frutescens var. japonica Hara, Perilla frutescens var. acuta Kudo, Perilla frutescens var. crispa Decaisne, Perilla frutescens var. for viridis Makino, Mosla punctulata Nakai, Mosla japonica Maxim, Mosla dianthera Maxim were analysized. The number of leaves per tiller and leaf size of perilla germplasms were more and bigger than those of mosla germplasms. Aroma degree of mosla germplasms was higher than aroma degree of perilla germplasms. Mosla germplasms could be utilized in the breeding for high aroma perilla lines. Otherwise, the softness of perilla germplasms was higher than that of mosla germplasms. In case of oil and protein contents, perilla germplasms was higher than mosla germplasms, however compositions ratio of fatty acid, especially linolenic acid of mosla germplasms was higher than that of perilla germplasms, therefore mosla germplasms could be utilized as breeding materials with high linolenic acid for industrial oil. The linolenic acid with excellent quality and unsaturated fatty acid showed negative correlation with oil content, protein content and saturated fatty acids.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.143-143
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• 2017

Wheat is one of the most important crops in production and consumption. Despite increasing of importance, the self-sufficiency of wheat is less than 2% in Korea. To improve yield potential and broaden the genetic pool of common wheat in Korea, introduction of alien germplasms into the Korean wheat breeding program is suggested. For effective utilization of the germplasm, we introduced total 1,195 germplasms from the world, which were provided by National Plant Germplasm System (NPGS, USDA) and evaluated the yield, field performances and agronomic traits for 8 years. Among 55 countries, germplasms from Canada, Ethiopia, Mexico and United States accounts for 78%, especially germplasms collected from United States accounts for 50%. Yield comparison of germplasms and collected region analysis indicate that the high yielding germplasms are collected from countries or states of particular range of latitude. The combination analysis of the yield and agronomic traits and the geographical information of collected region will be utilized for improving Korean wheat breeding programs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.4
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• pp.319-326
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• 2010

Safflower (Carthamus tinctorius L.) is a herb primarily distributed throughout in the world. We have used the inter-simple sequence repeats (ISSR) technique to investigate the phylogenetic relationships and genetic diversity of C. tinctorius. Of all germplasms, 88.7% were polymorphic among all germplasms. Mean genetic diversity within germplasms was very low (0.048). The Turkey germplasm had the highest expected diversity (0.082) and Australia germplasm was the lowest (0.020). These values indicate that most of the genetic diversity of safflower is found among germplasms and there is a high among-germplasm differentiation. We found eight phenetic bands for determining the specific marker of germplasm with SCAR markers. The regions of the Mediterranean Sea and India may be the most probable candidates for the origin of safflower. The tree showed four major clades: (1) European germplasms, (2) Azerbaijan, Egypt, and Ethiopia, (3) Australia, and (4) America.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.365-372
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• 2012

Native of soybean aphid (Aphis glycines Matsumura) is an Asia and aphid is one of the dangerous pests in soybean [Glycine max (L.) Merr.]. High density aphid populations can reduce crop production by causing severe damage. The objective of this study was evaluation of resistance to the soybean aphid in soybean cultivars and germplasms. A total of fifty five soybean cultivars or germplasms, including two susceptible and two resistant check varieties, were infested to evaluate their resistance in the field cage and greenhouse test by aphid colonies which derived from wild collected one soybean aphid biotype in Korea. The average number of reproduced soybean aphid was evaluated with 62.7 aphids in the resistant check variety PI 567598B and also estimated with 1,807 aphids for susceptible check variety Williams 82. In soybean varieties and germplasms, the average reproduced soybean aphid populations ranged from the lowest 497 aphids for Junjeori to the highest 3,862 aphids for Mansu. About seventy six percent of soybean cultivars and germplasms were shown high density soybean aphid populations when compared with another susceptible check variety PI 567543C in the field cage test. From the greenhouse test to evaluate aphid index, 87.3% of soybean cultivars or germplasms presented aphid index with 9.0. No soybean cultivars and germplasms were observed with soybean resistant phenotype when regarded a aphid resistant level as less than 10% aphid reproductions compared with susceptible check Williams 82. Although no Korean soybean cultivars were identified with resistant trait to the soybean aphid, we found one great resistant genetic resource PI 567598B in this study. This result will be helpful to further study for providing useful genetic information for soybean researchers.

• Korean Journal of Plant Resources
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• v.34 no.6
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• pp.575-599
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• 2021

The edible roots of radish (Raphanus sativus L.) are consumed worldwide. For characterization and evaluation of the agronomic traits and health-promoting chemicals in radish germplasms, new germplasm breeding materials need to be identified. The objectives of this study were to evaluate the phenotypic traits and glucosinolate contents of radish roots from 110 germplasms, by analyzing correlations between 10 quantitative phenotypic traits and the individual and total contents of five glucosinolates. Phenotypic characterization was performed based on descriptors from the UPOV and IBPGR, and glucosinolate contents were analyzed using liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode (MRM). Regarding the phenotypic traits, a significant correlation between leaf length and root weight was observed. Glucoraphasatin was the main glucosinolate, accounting for an average of 71% of the total glucosinolates in the germplasms; moreover, its content was significantly correlated with that of glucoerucin, its precursor. Principal component analysis indicated that the 110 germplasms could be divided into five groups based on their glucosinolate contents. High levels of free-radical scavenging activity (DPPH) were observed in red radishes. These results shed light on the beneficial traits that could be targeted by breeders, and could also promote diet diversification by demonstrating the health benefits of various germplasms.

• Korean Journal of Plant Resources
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• v.35 no.6
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• pp.785-795
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• 2022

Traditional germplasms are unsuitable for mechanized production, limiting adzuki bean production. The creation of cultivars that can be harvested by mechanized means is an urgent task for breeders. The bottom pod height (BPH), lodging resistance, and synchronous maturing of adzuki beans are critical factors for the reduction of losses due to mechanized harvesting. In this study, 14 traits of 806 adzuki bean accessions were analyzed. All growth stages and the yield, lodging score, and synchronous maturing correlated negatively with the BPH. These negative correlations reflect the increased difficulty of breeding to simultaneously satisfy the needs for no lodging, high synchronous maturing rates, BPHs > 10 cm, and high yield. We screened three germplasms with no lodging, high synchronous maturing rates, and BPHs > 10 cm that were used as mechanization-adapted breeding material for crossing with high-yield cultivars. Agronomic trait diversity in adzuki beans was also examined in this study. Principal component and cluster analyses were conducted for 806 germplasms resulting in three clusters with the yield and three growth stage traits serving as the main discriminating factors. Cluster 1 included high-yield germplasms with the number of pods per plant and the number of seeds per pod being the major discriminant factors. Cluster 2 included germplasms with long growth periods and large 100-seed weights while cluster 3 contained germplasms with high BPHs. In general, the characteristics that make mechanical harvesting feasible and those assessed in this study could be utilized to choose and enhance adzuki beans production.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.466-472
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• 2016

This study analyzed the genetic diversity of 115 pear germplasms using 15 SSR markers. Three to forty-one SSR alleles were detected for each locus with an average of 16 alleles per locus. The average availability of markers was 0.966. The average observed heterozygosity ($H_{obs}$) was 0.603 (range: 0.140 to 0.929). The average expected heterozygosity ($H_{exp}$) was 0.718 (range: 0.463 to 0.904). The average polymorphism information content (PIC) was 0.692 (range: 0.403 to 0.897). The genetic relationships of pear germplasms were classified into two major groups by geographic origins and genetic characteristics according to genetic distance. The first group was composed of European pear belonging to Pyrus communis. The second group consisted of P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana, interspecific hybrids, and unclear germplasms. The results of this study suggest that genotype analysis of pear germplasms using SSR markers can identify the genetic diversity of germplasms, and can be used to provide basic information for pear breeding.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.96-96
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• 2017

Rice is a staple food for nearly half of the world's population, with more than 10,000 rice varieties providing almost one-quarter of the global per capita dietary energy supply. Grain size, panicle size and branch number, grain number in a panicle are directly associated with rice productivity. Recently several genes which increase grain yield were identified through map-based cloning. Gn1a, Cytokinin oxidase, is a major grain number QTL and regulates grain number per panicle. Dep1 increases panicle branching and reduced rachis length. SCM2 (APO1) was identified by a QTL for culm strength and increased spikelet number. OsSPL16 (GW8) controls grain size and shape and then increases 1000-weight of seed. In here, to identify genotype of genes related to yield in 400 of rice germplasms possessed in National Institute of Crop Science, we had first chosen 4 of well-known genes related to yield; Gn1a, Dep1, SCM2, and OsSPL16. Among these germplasms, 195, 382, 165, and 353 of germplasms harbored the dominant type of Gn1a, Dep1, SCM2, and OsSPL16, respectively. We grouped these germplasms into a total of 10 groups using genotypes of Gn1a, Dep1, SCM2 and OsSPL16. Most rice germplasms belong to group 1, harbored Gn1a, dep1, gw8 and APO1, and group 10, harbored gn1a, Dep1, GW8 and apo1. Hanareum2 is the highest productive cultivar in Korea but do not have dominant type OsSPL16, so belong to group 1. On the other hand, in the case of Unkwang, belongs to group 10, which has dominant type of OsSPL16 but do not have the remaining genes. We can grasp the differences in rice germplasms through the Profiling of genes related to these grain yield, which will be useful for cross-breeding to integrate grain yield genes. We are continuously observing the genotype and phenotype of rice that possesses grain yield genes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.2
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• pp.142-146
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• 2005

Thirty-two germplasms of Korean adlay landraces were examined to analyse the genetic relationship through the amplified fragment length polymorphism (AFLP) approach. Total number of AFLP products generated by 12 selective primer combinations was 882. The number of polymorphic fragments by each primer combination greatly varied from 4 to 51 with a mean of 20.3, bands visible on the polyacrylamide gel. A genetic similarity coefficient was used for cluster analysis following UPGMA (unweighted pair grouping method of averages) method. The resulting clusters were represented in the form of a dendrogram. The clustering was not tight in the dendrogram. There was generally no clear grouping of the adlay according to the geographic regions in which germplasms were collected. The present AFLP analysis imply that although Korean adlay displayed a larger amount of AFLP variation within germplasms, the variation was shown independently without reflecting a clinal variation. This study demonstrated that AFLP method can be used to examine the genetic relationships among different germplasms of adlay.

• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.558-562
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• 2007

The high-performance liquid chromatography(HPLC) method for the determination of trans-resveratrol in 34 germplasms and processing methods of peanut seeds has been modified. Peanut germplasms contained trans-resveratrol contents of $0.14{\sim}4.96{\mu}g/g$, but findings for the testa color were not significant. However, two germplasms, 'KIGAN' and 'CS1', contained more trans-resveratrol contents than the other germplasms. The contents of their were $2.26{\mu}g/g\;and\;4.96{\mu}g/g$. The tested processing methods caused no significant changes in trans-resveratrol contents. The contents of fresh, boiled, and roasted peanuts were 0.36, 0.32, and $0.40{\mu}g/g$, respectively in cv. Palkwang, and 0.22, 0.22, and $0.26{\mu}g/g$, respectively, in cv. Jakwang. Differences were not significant among fresh, boiled, and roasted peanuts. The grains of 'Palkwang' and 'Jakwang' contained trans-resveratrol contents of $0.34{\mu}g/g\;and\;0.24{\mu}g/g$, and testa contained $1.12{\mu}g/g\;and\;1.00{\mu}g/g$, respectively. However, when comparing absolute quantity, the trans-resveratrol contents appears to be approximately $3{\sim}4$ times higher in the testa than in the grain of the peanut, although the total contents were not different because the ratio of testa was low in peanut seeds.