• Korean Journal of Medicinal Crop Science
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• v.25 no.4
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• pp.209-216
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• 2017

Background: Dehisced ginseng seeds need to be stored at cold temperatures for around 3 months to break their physiological dormancy, and thus, to aid in gemination. In the presence of high moisture in such an environment, seed spoilage and pre-germination may lower seed quality and productivity. To improve seed quality during cold-stratification, the effects of seed dehydration and temperature were tested. Methods and Results: In early December, dehisced ginseng seeds were dehydrated at 4 different levels and stored at $2^{\circ}C$ $-2^{\circ}C$, and $-20^{\circ}C$ for 3 months. Germination was carried out on the filter papers moistened with distilled water; emergence of root, shoot, and seed spoilage were assessed. Seed viability was examined by the tetrazolium test. More than 90% of the seeds stored at $2^{\circ}C$ and $-2^{\circ}C$ without drying or endocarp dehydration germinated, but seeds that were dehydrated to have a moisture content (MC) below 31% showed poor germination and lost their viability. In addition, the seeds stored at $-20^{\circ}C$ failed to show effective germination. Conclusions: Seed storage after endocarp dehydration might help to improve seed quality and increase seedling's ability to stand during the spring-sowing of ginseng.

• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.163-165
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• 1985

In assay of antifungal activity of fatty acids which slightly soluble in water against phytopathogenic fungi in vitro, it is necessary that evenly disperse fatty acid to assay in culture medium for mycelial growth or spore germination of test fungus. Acetone was selected as a dispersant in such an in vitro test because it is not only used as a solvent for fats, oils and waxes but also miscible with water. In this study to detect practically usable concentration of acetone in culture medium for test fungus, the results showed that both mycelial growth and spore germination of each test fungus were significantly inhibited on the culture media with 10% of acetone, while they were slightly inhibited on the culture media with 5% of acetone. Even though both mycelial growth and spore germination were influenced on the culture media with 5% of acetone, it is not considered that the use of culture medium with 5% of acetone make a mistake in assay of antifungal activity of fatty acids against phytopathogenic fungi.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.417-424
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• 1987

The seeds of three barley varieties of which initial seed vigor were different were used to measure seed vigor of accelerated aging techniques. A vigor index derived from the product of percent germination and plumule length was used to estimate seed vigor. The index was compared with the results of other tests. The results of warm germination test well suited to the measurements of seed vigor at the advanced stages of seed deterioration. Other vigor measurements except ATP and GADA values, showed close relationships with the vigor index. The measurements of plumule length in cold test and tetrazolium test were found to be appropriate for predicting seed quality.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.276-283
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• 1997

This experiment was conducted to investigate the optimum temperature and the effects of sulfuric acid, flowing water and $GA_3$ on the germination of Bupleurum falcatum. At the same time, the effect of germination-related compounds in Bupleurum falcatum seed was tested. Among the tested temperatures $(15,\;20,\;25\;and\;30^{\circ}C)$ the highest germination rate was obtained at $15^{\circ}C$. The optimum soaking time of sulfuric acid was 5 minutes at the concentration of both 0.1 and 1.0%. At the same time, the germination rate was highest when Bupleurum falcatum seed was washed for 2 days in the flowing water before germination test. The effect of $GA_3$ on the germination was highest at 100 ppm out of 10, 50 and 100 ppm. Regardless of treatments, the germination rate of Jeongseon species was higher than that of Mishima species. SEM photographs revealed that most of germination-inhibiting substances in the seed coat were washed away by rinsing in the flowing water for 2 days thus the seed coat became more smooth. The 4, 000 ppm alcohol extract of Bupleurum falcatum seeds inhibited the germination of lettuce seeds as much as 41.7% in Jeongseon species and 58.3% in Mishima species. The higher extract concentration from seed of Bupleurum falcatum, the lower the germination of lettuce seeds was obtained. Out of phenolic acids, the contents of orchinol, pyrogallol, and p-hydroxybenzoic acid were highest in both varieties. Compared with Mishima species, Jeongseon species showed much higher content of salicylic and vanillic acid.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.175-181
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• 2001

Dormancy of intact zoysiagrass seeds hinders its seed propagation in which presowing seed treatment must be done to overcome it. The study was done to remodel the presowing treatments of zoysiagrass seeds by determining the treatment effects of seedcoat softening, prechilling, drying and combined light treatment with them or during germination stage. Seedcoat softening with different chemicals (NaOH 20%, KOH 20%, $H_2SO_4\;8M, acetone 20%) and periods (10, 30, 60 min.), prechilling with different periods at 3$^{\circ}C$, and drying with 4 hours at 35$^{\circ}C$ was done. Light sources were also applied with 660 nm red LED light, commercial fluorescent and incandescent light and darkness. Germination test was done in alternative day and night temperature of 35 and 2$0^{\circ}C$ to check the daily germination rates. With seeds treated with NaOH 20% for 30 minutes, red and incandescent light showing similar responses inclined their germination rates compared to dark conditions. Drying resulted in its higher rate than non-drying but fluorescent light treatment forced during drying more enhanced the rate than red light. While mean germination rate considering all the seedcoat softening treatment periods was the greatest in $H_2SO_4$ in comparison with NaOH, KOH and acetone, NaOH treatment for 30 minutes showed the best rate among the all the treatment levels. In the conditions of sowing them to no-mulching field, presowing treatment procedure of zoysiagrass seeds was remodeled: the seedcoat softening treatment with NaOH 20% for 30 minutes was done, washed, and chilled at 3$^{\circ}C$ for 2 weeks before dried under 4 hour fluorescent light illumination at 35$^{\circ}C$.

• Journal of Life Science
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• v.32 no.8
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• pp.641-646
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• 2022

There are concerns about the environmental release of living modified organism (LMO) maize created to increase yields. In fact, there are cases in which LMO crops for feed have been leaked in Korea to form autoite colonies, and concerns about LMO spill are intensifying. In this study, the possibility of environmental outflow and occurrence of native organisms was analyzed using maize feed and seeds distributed in Korea. In the evaluation of the possibility of spontaneous occurrence of maize in the event of an unintentional release of maize feed made by crushing maize, the incidence rate of maize was 0.01%, which was extremely low compared to the germination rate of maize seeds. A survey of the dormant rate of maize showed that all maize seeds collected every month were dead. In the germination rate test by temperature using Daehak wax corn and Kwangpyeongok, high germination rates were found at 20℃ and 30℃, and relatively low germination rates were found at 10℃ and 40℃. In addition, all germination tests showed a higher germination rate Daehak wax corn than Kwangpyeongok. The difference between domestic and overseas cultivation maize was confirmed through a survey on the agricultural properties of three varieties of maize. The data obtained through this experiment could be the basis for the evaluation of the weediness potential of environmental risk assessment and technology to suppress the occurrence of autoite in preparation for future LMO spills.

• Korean Journal of Weed Science
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• v.8 no.3
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• pp.258-264
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• 1988

Phenolic compounds present in upland weeds (Artemisia asiatica Nakai, Capsella bursa-pastoris (L.) Medik, Portulaca orleracea L. and Trifolium repens L.) which have shown allelopathic activity were determined using paper chromatography (PC) and high performance liquid chromatography (HPLC). Effect of the determined phenolic compounds on germination and post-germination growth of test plants was also investigated. Kinds of phenolic compounds determined by PC in the four weed species were greater in the aqueous extract than in the methanol extract. Ferulic acid was found in both extracts of the weeds studied, whereas benzoic acid was that the weeds commonly contained hydroquinone, p-hydroxybenzoic, ferulic and cinnamic acids. Out of the phenolic compounds determined by PC and HPLC p-hydroxybenzoic, cinnamic and ferulic acids inhibited germination and post-germination growth of radish and sesame. Inhibition of the phenolic compounds on the radicle growth was greater than on the germination of the test plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.2
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• pp.188-193
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• 2014

Seed viability testing provides valuable information for assessing seed lot germinability. However, most testing methods require destruction of seed prior to test. Because the dissected seeds for viability test cannot be used further evaluation, the nondestructively X-ray photography technique that can be applied for the evaluation of seed quality has been developed. In order to know the validity and accuracy of X-ray photography technique in seed evaluation test that conducted to remove the abnormal seed from a seed lot, we have compared the results from tetrazolium viability test, germination test and X-ray contrast method in cotton. Metallic salts treatment increased the efficiency of X-ray photographic method by enhancing the penetration of X-ray in abnormal or damaged seeds rather than normal seeds that have strong and well-organized tissues in seed. Cotton seeds presoaked for 16 hr in distilled water followed by soaking into metallic salt solution (5% NaI in water) for 60 min were easily classified seeds into dead seed and viable seed based on the radiography images obtained by X-ray radiation. We concluded that soft X-ray photography was reliable to find out the various defective characters due to heat and mechanical damage of seeds.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.04a
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• pp.38-41
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• 2001

It has been reported that some plants have the potential to metabolize the 2,4,6-trintroluene (TNT) in contaminated soils, sediments and natural water. In this study, the effects of TNT on germination and early seedling development of Abutilion avicennae was characterized in a germination test. Concentration up to 80 mg/L TNT did not affect germination but root and shoot growth, and fresh biomass decreased as TNT concentration increased. A series of axenic hydroponical batch culture of Abutilion avicennae at various initial TNT concentration was used to determine its transformation kinetics, to identify products formed, and to evaluate phytotoxic effects on the TNT transformation process. At higher initial TNT concentrations, TNT removal rate constant decreased, however, total amount of TNT removed was increased in the culture media. Reductive transformation products of TNT were not detected in the plant culture media but higher concentrations of reduced metabolites were detected in the root and stem extracts of plant material at the 7 days of batch incubation. From these results we concluded that Abutilion avicennae has an intrinsic capacity for taking up and transforming TNT.

• Journal of Ginseng Research
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• v.11 no.1
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• pp.77-83
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• 1987

The effects of ginseng saponin on the germination and early growth of rice seeds (Oryza sativa L.) were investigated. The early growth (length) of test rice seeds which were rinsed for 60 hrs in $10^{-4}$% saponin solution prior to transplantation to water agar bed was about 20% faster than that of control seeds, It was also found that the amylase activity of the seeds was most active when the seeds were rinsed in 10-4% saponin solution for 48 hrs. In vitro investigation showed that the amylase activities were stimulated about 30% by the treatment of $10^{-5}$% saponin solution compared with control group. From the above results, it seems that the ginseng saponin might activate amylase of rice seed during germination, resulting in rapid growth of rice.