• Title/Summary/Keyword: Germination Percentage

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The Effects of Experimental Warming on Seed Germination and Growth of Two Oak Species (Quercus mongolica and Q. serrata) (온난화 처리가 신갈나무(Quercus mongolica)와 졸참나무(Q. serrate)의 종자발아와 생장에 미치는 영향)

  • Park, Sung-ae;Kim, Taekyu;Shim, Kyuyoung;Kong, Hak-Yang;Yang, Byeong-Gug;Suh, Sanguk;Lee, Chang Seok
    • Korean Journal of Ecology and Environment
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    • v.52 no.3
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    • pp.210-220
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    • 2019
  • Population growth and the increase of energy consumption due to civilization caused global warming. Temperature on the Earth rose about $0.7^{\circ}C$ for the last 100 years, the rate is accelerated since 2000. Temperature is a factor, which determines physiological action, growth and development, survival, etc. of the plant together with light intensity and precipitation. Therefore, it is expected that global warming would affect broadly geographic distribution of the plant as well as structure and function ecosystem. In order to understand the effect of global warming on the ecosystem, a study about the effect of temperature rise on germination and growth in the plant is required necessarily. This study was carried out to investigate the effects of experimental warming on the germination and growth of two oak species(Quercus mongolica and Q. serrata) in temperature gradient chamber(TGC). This study was conducted in control, medium warming treatment($+1.7^{\circ}C$; Tm), and high warming treatment ($+3.2^{\circ}C$; Th) conditions. The final germination percentage, mean germination time and germination rate of two oak species increased by the warming treatment, and the increase in Q. serrata was higher than that in Q. mongolica. Root collar diameter, seedling height, leaf dry weight, stem dry weight, root dry weight, and total biomass were the highest in Tm treatment. Butthey were not significantly different in the Th treatment. In the Th treatment, Q. serrata had significantly higher H/D ratio, S/R ratio, and low root mass ratio (RMR) compared with control plot. Q. mongolica had lower RMR and higher S/R ratio in the Tm and Th treatments compared with control plot. Therefore, growth of Q. mongolica are expected to be more vulnerable to warming than that of Q. serrata. The main findings of this study, species-specific responses to experimental warming, could be applied to predict ecosystem changes from global warming. From the result of this study, we could deduce that temperature rise would increase germination of Q. serrata and Q. mongolica and consequently contribute to increase establishment rate in the early growth stage of the plants. But we have to consider diverse variables to understand properly the effects that global warming influences germination in natural condition. Treatment of global warming in the medium level increased the growth and the biomass of both Q. serrata and Q. mongolica. But the result of treatment in the high level showed different aspects. In particular, Q. mongolica, which grows in cooler zones of higher elevation on mountains or northward in latitude, responded more sensitively. Synthesized the results mentioned above, continuous global warming would function in stable establishment of both plants unfavorably. Compared the responses of both sample plants on temperature rise, Q. serrata increased germination rate more than Q. mongolica and Q. mongolica responded more sensitively than Q. serrata in biomass allocation with the increase of temperature. It was estimated that these results would due to a difference of microclimate originated from the spatial distribution of both plants.

Seed Production Studies in Italian ( Lolium Multiflorum Lam. Italicum ) II. Moisture content , seed weight , shattering and germination in ripening process of Italian ryegrass (이탈리안 라이그라스의 종자생산에 관한 연구. 제2보. 사배체 품종의 종실발달에 따른 천립중 , 탈립 , 발아율 및 수분함량의 변화)

  • 류종원;강정훈;박병훈
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.1
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    • pp.79-83
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    • 1985
  • This experiment was carried out in order to determine the optimum harvesting time of tetraploid Italian ryegrass. Moisture content, 1000-seed weight, germination and seed shattering were investigated at six harvesting times after anthesis in 1984 at Suweon. The results are summarized as follows; 1. Seed moisture content was slowly decreased until 24 days and fastly decreased from 24 days after anthesis. The daily mean moisture loss during the ripening period was about 1.5 percentage. 2. The maximum 1000-seed weight was reached at 24 days after anthesis and 1000-seed weight of Waseking was heavier than that of Giant. 3. Shattering began at 17-20 days after anthesis and thereafter rapidly increased. 4. Germination was 50% at 10 days after anthesis and maximum germination was reached at 24 days. 5. Considering germination, 1000-seed weight and shattering, the optimum harvest time was 24 to 27 days after anthesis. At this time, moisture content was 55.0 to 43.5 percent and two to five seeds per ten spikes were shed by shaking at the vertical point.

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A Study on the Germination of Apple Rootstock Seed, Malus sieboldii (사과 대목 종자의 발아에 관한 연구)

  • Cheong, Sam Taek;Kim, Ik Hong;Jeun, Sang Hang;Kim, Min
    • Current Research on Agriculture and Life Sciences
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    • v.7
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    • pp.41-46
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    • 1989
  • The seeds of Malus sieboldii were collected in late fall of 1988 and stored in cold stratification condition for 15 weeks. Growth regulators such as $GA_3$, BA and ABA were treated to the seed to know germination capacity and seedling growth. The results were as follows. 1) Higher germination was achieved in $GA_3$ and BA plots, But ABA treatment showed lower germination percentage and physiological dwarf and also induced to the secondary dormancy condition in partly. 2) Average length of time for germination was delayed over 2 days in ABA plot when comparing with the other plots. 3) Average length of plumule and radicle also was inhibited by ABA treatment. This means lower seedling vigor. 4) Fresh and dry weight of the seeding were higher in BA plot while ABA plot was lower and undesirable seedling was produced. From above mentioned results, it is believed that ABA treatment to the seed induced the secondary dormancy and physiological dwarf pattern. Therefore, utilization of BA can be produced the normal seedling, the authors believed.

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Effect of Seed Priming on the Germination Performance and Membrane Integrity of Tomato(Lycopersicon esculentum Mill.) Seeds (Priming 처리에 의한 토마토 종자의 발아력과 Membrane Integrity에 미치는 영향)

  • Kang, Jum-Soon;Son, Beung-Gu;Ahn, Chong-Kil
    • Journal of Bio-Environment Control
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    • v.12 no.4
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    • pp.221-227
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    • 2003
  • The objective of this research was to determine the effect of seed priming on membrane integrity during poriming and germination. Among the five chemicals, $KNO_3$at 150 mL gave the shortest $T_{50}$ (days required to reach 50% of the final germination percentage). Compared to unprimed, the seeds primed with 150 mL $KNO_3$ at 20$^{\circ}C$ for 4 days had reduced $T_{50}$ values when germinated at 15$^{\circ}C$. These results indicated that seed priming is an effective way for rapid and synchronized germination, especially at low temperature. Changes in conductivity of priming solutions during the 4-days period of priming were highly dependent upon the priming agents. Conductivity of the $KNO_3$ and $K_3PO_4$ solution slowly declined during the first 3 hours ad then increased Amount of amino acids, sugars and proteins exuded from seeds into $KNO_3$ solution were less than those into distilled water and $K_3PO_4$. All the results suggested that the $KNO_3$ priming play a positive role in regulating the permeability of cell membranes.

Stimulation of Seed Germination of Korean Lawn grass (Zoysia japonica Steud.) by Sodium Hypochlorite Treatment (Sodium Hypochlorite 처리(處理)에 의한 한국잔디 종자(種子)의 발아촉진(發芽促進))

  • Ku, Ja Hyeong;Lee, Jong Suk;Lee, Young Bok
    • Korean Journal of Agricultural Science
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    • v.11 no.2
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    • pp.201-206
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    • 1984
  • In order to obtain an easy and effective scarification method for stimulating the seed germination of Korean lawn grass (Zoysia japonica Steud.), sodium hypochlorite (SH), which is used as a commercial bleach and a seed disinfestant, was tested as a chemical scarifier instead of potassium hydroxide (KOH). 1. Scarification with 4% SH for 2 hours followed by 8-hour water-rinse stimulated seed germination more effectively compared to generalized scarification method by KOH treatment. 2. The degree of stimulating seed gemination was different by the various water-rinsing time after SH treatment, but the optimum rinsing time was about 4 to 8 hours. 3. GA treatment after SH scarification stimulated seed germination. However, the stimulation effect of GA was not showed in non-scarified control. 4. SH treatment significantly decreased the percentage of the infection of microflora in seed samples compared to KOH treatment. 5. Under practical seed scarification conditions for stimulating germination of Korean lawn grass seed. SH may have advantages over KOH with respect to ease of preparing, securing and handling.

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Effects of Salt and Drought Stresses on Seed Germination and Gene Expression Pattern in Tall Fescue (염과 건조 스트레스 조건에서 톨 페스큐의 종자 발아율과 유전자 발현 변화분석)

  • Lee, Sang-Hoon;Lee, Ki-Won;Choi, Gi Jun;Kim, Ki-Yong;Ji, Hee Jung;Hwang, Tae Young;Lee, Dong-Gi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.2
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    • pp.114-119
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    • 2014
  • Salinity and drought stresses are probably the most significant abiotic factor limiting plant's growth, also negatively affect seed germination and early seedling development. To study on effect of NaCl and PEG stress on seed germination and gene expression pattern of tall fescue, the levels of NaCl and PEG-induced water stresses were determined in first experiment. Different concentration of NaCl (0 to 350 mM) and PEG (0 to 30%) were used for seed treatment. Seed Germination percentage reduced with increasing osmotic potential of growth medium either due to NaCl or PEG. Seeds were not germinate at 350 mM NaCl or 30% PEG treatment. On the basis of the results, Kentucky31(E-) had more resistant than Fawn in both stress conditions. Furthermore, we have used an annealing control primer-based differential display reverse transcription-polymerase chain reaction method to identify salt- and drought stress-induced differentially expressed genes (DEGs) in tall fescue leaves. Using 120 annealing control primers, a total of 4 genes were identified and sequenced. The possible roles of the identified DEGs are discussed in the context of their putative role during salinity and drought stresses.

Effect of Alfalfa Extract, It's Concentration and Absorbents on Germination and Growth of Alfalfa (알팔파 추출물, 추출농도 및 흡착제 처리가 알팔파 발아와 생육에 미치는 영향)

  • Ill Min, Chung;Ki-June Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.5
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    • pp.619-628
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    • 1995
  • Crude aqueous extracts from dried leaves, stems, roots, and flowers from both field grown and greenhouse grown alfalfa plants inhibited alfalfa seed germination and seedling growth. The degree of inhibition was greater in the field grown plant extracts. Flowers extract of field grown plant most inhibited alfalfa germination and seedling growth. In the concentration study, the highest concentration of extract (9.0%, w/v) significantly inhibited total alfalfa seed germination by 50% as compared to control. In partitioning study using pot hydroponic culture of plant biomass into leaves, stems, root, LAR products of LWR and SLA exhibited significant variation among four species. This result support that the inhibitory effect of autotoxic substances presenting in alfalfa tissue may be possible interference with the patitioning of biomass into leaf component relative to the total biomass produced by the alfalfa plant. Toxicity of extract was not reduced by adding activated charcoal, Dowex-50W, amberlite to the extract. Toxic substances existing in most plant tissues but mainly above ground foliage are water soluble and stable and may persist in old alfalfa fields. Thus, it is recommended to remove as much as possible of the above growth parts, especially vegetative stage, before one tries to re-establish alfalfa in former field of alfalfa.

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Development in Planting Porous Block for Revegetation (녹화용 다공질 식재 블럭의 개발)

  • Ahn, Young-Hee;Choi, Kyoung-Young
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.5 no.4
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    • pp.1-9
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    • 2002
  • This study is carried out to make the environmentally affinitive porous planting block for revegetation and to make a effective program for greening plans. The summary is shown below. 1. In order to get stronger intensity and distribute proper porosity in the block for planting, the cements mixed with fine soil were used and the finer in soil grains gives the stronger in intensity of the cements. Use of the furnace slag cements instead of the portland cements showed relatively stronger in intensity of the block. The intensity of the block became stronger when the mixed ratio of the cements to soil is 5 : 1, but the pore space ratio was lower. The percolate pH of the portland cements after one month of treatment was 13.1 but the percolate pH of the furnace slag cements was shown lower. To mold proper porous planting blocks, the proper combination of additives such as the dehydrating agent, elastic agent and adhesives into the mixture of cements and soil gives better effectives. 2. After molding the porous planting blocks, it gave a better result when the grains of the filler made of peat moss, upland soil and compound fertilizer were smaller than 2 mm in size. Shaking of the filling materials also gave the better result, but it took more time and cost much more. Therefore, it was better when the filling materials were mixed with water first then flew down for stuffing. 3. It was necessary to cover with soil after seeding or planting on the porous planting blocks. The proper thickness of the soil to help root development and keep moisture is about 3~5 cm. 4. The plants for planting on the porous planting block were required stronger in the growth condition of their roots and their environmental adaptability. The average germination percentage and rate of Platycodon grandiflorum on the porous planting block were 88.8% and 85% accordingly and their rate is very uniform. The germination rates of Dianthus superbus var. longicalycinus and Taraxacum officinale were more than 50%. These grass species, Chelidonium majus var. asiaticum, Lysimachia mauritiana and Scabiosa mansenensis were the suggested biennial grasses in the planting area where exchanging of the seedling or nursery plants was not necessary because their germination rates were 59.3, 45.6 and 40.3% accordingly. Viola kapsanensis, Chrysanthemum sp., Taraxacum sp. and Iris ensata var. spontanea are the grass species that could be used by seeding for greening. However, the germination rate of Solidago virga-aurea var. asiatica, Aster scaber and Lythrum anceps were lower than 10%. The coverage ratio of Ixeris stolonifera is more than 80% after 60 days seeding and the root length of most of species are more than 10 cm except Iris ensata var. spontanea and Platycodon grandiflorum because their root developed thicker than other species.

Studies on the Pathogenic Alternaria Isolated from Seedlings of Pinus rigida Mill. - part II (리기다 소나무 유묘(幼苗)에 기생(寄生)하는 병원성(病原性) Alternaria균(菌)에 관(關)한 연구(硏究) - 제2보(第二報))

  • Yun, J.K.
    • Journal of Korean Society of Forest Science
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    • v.6 no.1
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    • pp.19-23
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    • 1967
  • The fungus which belongs to Alternaria has been isolated from "damping-off" infected seedlings of pitch pine (Pinus rigida Mill.) in the nursery (seed bed) of Chung Puk College, Cheong-Ju. The author has carried out this study regarding to (1) the effects of H-ion concentration on the growth of fungus, (2) the effects of temperature and humidity on the conidia germination, (3) the effects of temperature and humidity on the conidia formation. The summary of obtained in this experiments is as follows: 1. The suitable H-ion concentration for growth of isolated fungus is in the range of PH 4.0-9.5, the most suitable of it is PH 6.5-8.0. 2. The germination of the conidia is suppressed at below $0^{\circ}C$ or at $40^{\circ}C$ and above, the range of optimum temperature for the germination is $5^{\circ}C-35^{\circ}C$, the percentage of germination is highest at $20^{\circ}C$. And under relative humidities of 10% or below, the germination is suppressed, favorable relative humidities $85%{\cdots}100%$. 3. The formation of the conidia is suppressed at below $10^{\circ}C$ or at $34^{\circ}C$ and above, the favorable condition of the condia formation is as follows; Temperature; $22^{\circ}C-28^{\circ}C$ Relative humidity:85%-100%.

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Ecological Studies on the Occurrence of Rice False Smut (벼 이삭누룩병(病)의 발생(發生) 생태(生態)에 관(關)한 연구(硏究))

  • In, Moo Seong;Park, Jong Seong;Yu, Seung Hun
    • Korean Journal of Agricultural Science
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    • v.12 no.2
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    • pp.242-252
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    • 1985
  • In order to get information on the ecology of rice false smut, germination ability and pathogenicity of sclerotia and chlamydospores of the pathogen, environmental conditions affecting the disease outbreak and varietal resistance have been investigated. 1. The degree of outbreak of rice false smut was higher in the upland rice in comparison with the paddy field rice in respect to the number of affected grains per ear, the size and weight of smut balls formed on affected grains as well as the ratio of sclerotial formation produced on smut balls. 2. Germination percentage and days required for germination of overwintered sclerotia placed on the soil surface in July were 81% and 19 days, respectively, while those of overwintered sclerotia treated in May were 60-70% and 41 days. Sclerotia placed on the soil surface or under 1 cm depth of the soil surface and incubated at $25-30^{\circ}C$ were germinated well, whereas those placed under 3 cm or 5 cm depth of the soil surface were not germinated at all. Germinability and stroma productivity of sclerotia were reduced when the sclerotia were cutted into small pieces. 3. The average number of stroma formed on a sclerotium was six and that of perithecia formed in a stroma was about 50 to 140. 4. Percentage of germination of chlamydospores on the yellow balls was very high and was decreased as the color of the balls being darken with maturation. 5. Panicle of rice plants were successfully infected by injection inoculation with suspention of ascospores and chlamydospores of the pathogen to the sheaths at the booting stages, while seeding infection by spraying with suspensions of chlamydospores was unsuccessful. 6. More number of infected grains was distributed on basal parts of an affected ear than that of infected ones distributed upper parts of the ear, when the affected ear was divided into five parts from its basal portion to the apical of the ear. 7. The occurrence of the disease was more severe in the late maturing varieties of rice in comparison with the early maturing varieties. 8. When the level of nitrogen applied was increased, the incidence of disease increased, and the infection percentage of the disease was increased as the transplanting date was delayed. 9. The weight of panicles and 1000 kernels and the ratio of ripenness were reduced, and the contamination degree of grains with chlamydospores were increased as the number of smut balls per panicle were increased.

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