• Reproductive and Developmental Biology
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• v.35 no.4
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• pp.399-405
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• 2011

The number of abandoned dogs is increasing with the worsening of the economy and the rising of feed value. It was becoming a serious social problem because of the disease transmission and destruction of natural ecosystems by abandoned dogs been wild animal. In order to solve these problems, companion dogs necessary to secure its own genetic information and to establish the systematic tracking system. Using multiplex-PCR method with 27 microsatellite marker (MS marker) divided 3 set, various alleles occurring to 6 dog breed (Labrador Retriever, German Shepherd, English Springer Spaniel, Belgian Malinois, Jindo Dog, PoongSan Dog) make use of markers to determine allele frequency and heterozygosity. MS marker FH2834 and FH2790 have only two allele and most were found in 13 alleles at FH3381 and FH3399. Average heterozygosity of MS marker is 0.534 and especially, heterozygosity represented the highest value of 0.765 at FH3381. So, it was recognized appropriate allele frequency for individual identification and paternity diagnosis in companion dogs. Using multiplex-PCR method with MS marker, various alleles occurring to dog breed make use of markers to deter mine individual identification and paternity diagnosis, traits associated biomarkers and breed-specific marker for faster, more accurate and ways to reduce the analysis cost. Based on this result, a scientific basis was established to the existing pedigree data by applying genetics additionally. Animal registration system is expected to be conducted nationwide in future. The method expects to very useful this system.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.8
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• pp.1078-1084
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• 2009

The Jindo dog is a Korean natural monument and is recognized by the Fédération Cynologique Internationale. A prominent feature is the diverse coat color within the breed. To analyze the genetic basis of variation in the Jindo coat color, we sequenced the protein-coding regions of the melanocortin 1 receptor gene (MC1R). The MC1R coding sequence was determined from 154 dogs in five breeds (Jindo, Labrador Retriever, English Springer Spaniel, Belgian Malinois, and German Shepherd). To confirm the genetic structure of sampled populations, we tested for Hardy-Weinberg equilibrium (HWE) and computed $F_{st}$ The sample populations did not significantly deviate from HWE. $F_{st}$ was 0.02 between white and fawn Jindo dogs; this was lower than $F_{st}$ between breeds. Six single nucleotide polymorphisms (SNPs) were detected in the MC1R coding region. Among the six SNPs, five were non-synonymous (S90G, T105A, Q159P, M264V, and R306ter) and one was synonymous SNP (Y298Y). From the SNPs, we predicted four haplotypes (H1, H2, H3, and H4) for Jindo MC1R. Jindo dogs had different haplotypes corresponding to different coat colors. H1 was frequently observed in white Jindo dogs with an odds ratio of 5.03 (95% CI: 2.27-11.18, p<0.0001), whereas H2 and H4 were observed only in fawn Jindo dogs. Our findings indicate that SNP haplotype can influence coat color. Knowledge of MC1R haplotypes can help discriminate white and fawn coats in Jindo dogs. We hope this report will trigger more research into the genetics of this traditional Korean dog and will be a reference for dogs of Asian origin. Also, our results will provide a useful genetic marker for Jindo dog breeders who have selected for specific colors.

• Journal of Veterinary Clinics
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• v.34 no.3
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• pp.165-171
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• 2017

A cross-sectional study was conducted to investigate the prevalence of canine influenza (CI) infection in pet dogs and canine parvovirus (CPV) infection in street dogs of different age and sex by collecting rectal and nasal swab samples from three districts, Dhaka, Mymensingh and Sirajgonj, in Bangladesh using a RapiGEN$^{(R)}$ Canine Influenza Virus Ag Test kit and RapiGEN$^{(R)}$ Canine Parvovirus Ag Test Kit. Out of 114 rectal swabs and 115 nasal swab samples, the overall prevalence of CI and CPV was found to be 11.30% and 32.45%, respectively. The prevalence of parvovirus infection was found to be significantly higher in puppies and dogs 6 months of age (50.0%) than those > 24 months of age (p = 0.005). The prevalence was also higher in males (34.42%) than females (30.18%). The prevalence of CI was higher (30.43%) in dogs up to 6 months of age (p = 0.011) than 6-12 month (7.93%) and 12-18 month (6.66%) old dogs. Moreover, the prevalence of CI was found to be higher in males (16.10%) than females (5.66%). The prevalence of CPV infection also varied significantly in different study areas (p = 0.0029), with 12.72%, 12.5% and 7.14% of dogs found to be CI positive in Dhaka, Mymensingh and Sirajgonj, respectively. Overall, the highest prevalence of CI was found in local breeds (6.08%) followed by German shepherds and Keeshonds (1.73%), and Bloodhounds and Terriers (0.86%). Additionally, there were more positive CI found in unvaccinated dogs (14.81%) than vaccinated (2.94%) dogs. Dogs with flu-like symptoms were more positive (19.23%) for CI relative to those without flu-like symptoms (4.76%) samples. Overall, the results of this study indicate that canine vaccination should be initiated to prevent the occurrence of diseases and that regular monitoring should be continued in Bangladesh.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.40 no.5
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• pp.300-307
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• 2007

This study investigated the effects of dietary supplementation with hot water extracts (HE) of selfheal (Prunella vulgaris) and Lactobacillus rhamnosus culture fluid (LF) on the growth and non-specific immune responses of juvenile olive flounder. A total of 270 fish ($5.07{\pm}0.01g$, average $weight{\pm}SD$) were divided randomly into nine groups, and three groups were fed one of three isonitrogenous (51% crude protein) and isocaloric (17.6 MJ/kg) diets. The diets contained no supplement, 50 mL hot water extract, or 50 mL L. rhamnosus fluid (designated as diets CON, HE and LF, respectively). During the 8-week feeding trial, the growth, feed utilization and survival of the fish were not significantly affected by the experimental diets. There were no significant differences in the hematocrit and hemoglobin of fish fed each of the experimental diets. However, the serum alanine aminotransferase and aspartate aminotransferase activity were lower with the dietary supplements containing HE and LF. The alanine aminotransferase activity of fish fed the HE diet was significantly lower than that of fish fed the control diet. The hematocrit, hemoglobin, and nitroblue tetrazolium (NBT) activity were higher 3 hr after feeding than 24 hr afterwards. The NBT activity of fish fed the HE and LF diets were significantly higher than that of fish fed the control diet 3 hr after feeding. The findings suggest that dietary supplementation with HE and LF could enhance the nonspecific immune responses of juvenile olive flounder.

• Bulletin of the Korean Chemical Society
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• v.32 no.1
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• pp.201-207
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• 2011

Peroxisome proliferator-activated receptors (PPARs) are members of nuclear receptors and their activation induces regulation of fatty acid storage and glucose metabolism. Therefore, the $PPAR\gamma$ is a major target for the treatment of type 2 diabetes mellitus. In order to generate pharmacophore model, 1080 known agonists database was constructed and a training set was selected. The Hypo7, selected from 10 hypotheses, contains four features: three hydrogen-bond acceptors (HBA) and one general hydrophobic (HY). This pharmacophore model was validated by using 862 test set compounds with a correlation coefficient of 0.903 between actual and estimated activity. Secondly, CatScramble method was used to verify the model. Hence, the validated Hypo7 was utilized for searching new lead compounds over 238,819 and 54,620 chemical structures in NCI and Maybridge database, respectively. Then the leads were selected by screening based on the pharmacophore model, predictive activity, and Lipinski's rules. Candidates were obtained and subsequently the binding affinities to $PPAR\gamma$ were investigated by the molecular docking simulations. Finally the best two compounds were presented and would be useful to treat type 2 diabetes.

• Bulletin of the Korean Chemical Society
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• v.30 no.5
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• pp.1152-1156
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• 2009

Silent information regulator 2 (Sir2) or sirtuins are NAD(+)-dependent deacetylases, which hydrolyze the acetyllysine residues. In mammals, sirtuins are classified into seven different classes (SIRT1-7). SIRT1 was reported to be involved in age related disorders like obesity, metabolic syndrome, type II diabetes mellitus and Parkinson’s disease. Activation of SIRT1 is one of the promising approaches to treat these age related diseases. In this study, we have used HipHop module of CATALYST to identify a series of pharmacophore models to screen SIRT1 enhancing molecules. Three molecules from Sirtris Pharmaceuticals were selected as training set and 607 sirtuin activator molecules were used as test set. Five different hypotheses were developed and then validated using the training set and the test set. The results showed that the best pharmacophore model has four features, ring aromatic, positive ionization and two hydrogen-bond acceptors. The best hypothesis from our study, Hypo2, screened high number of active molecules from the test set. Thus, we suggest that this four feature pharmacophore model could be helpful to screen novel SIRT1 activator molecules. Hypo2-virtual screening against Maybridge database reveals seven molecules, which contains all the critical features. Moreover, two new scaffolds were identified from this study. These scaffolds may be a potent lead for the SIRT1 activation.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.10
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• pp.4717-4737
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• 2017

Today, smart grids, smart homes, smart water networks, and intelligent transportation, are infrastructure systems that connect our world more than we ever thought possible and are associated with a single concept, the Internet of Things (IoT). The number of devices connected to the IoT and hence the number of traffic flow increases continuously, as well as the emergence of new applications. Although cutting-edge hardware technology can be employed to achieve a fast implementation to handle this huge data streams, there will always be a limit on size of traffic supported by a given architecture. However, recent cloud-based big data technologies fortunately offer an ideal environment to handle this issue. Moreover, the ever-increasing high volume of traffic created on demand presents great challenges for flow management. As a solution, flow aggregation decreases the number of flows needed to be processed by the network. The previous works in the literature prove that most of aggregation strategies designed for smart grids aim at optimizing system operation performance. They consider a common identifier to aggregate traffic on each device, having its independent static aggregation policy. In this paper, we propose a dynamic approach to aggregate flows based on traffic characteristics and device preferences. Our algorithm runs on a big data platform to provide an end-to-end network visibility of flows, which performs high-speed and high-volume computations to identify the clusters of similar flows and aggregate massive number of mice flows into a few meta-flows. Compared with existing solutions, our approach dynamically aggregates large number of such small flows into fewer flows, based on traffic characteristics and access node preferences. Using this approach, we alleviate the problem of processing a large amount of micro flows, and also significantly improve the accuracy of meeting the access node QoS demands. We conducted experiments, using a dataset of up to 100,000 flows, and studied the performance of our algorithm analytically. The experimental results are presented to show the promising effectiveness and scalability of our proposed approach.

• Journal of Life Science
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• v.29 no.6
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• pp.637-644
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• 2019

This study was conducted to analyze the genetic characteristics of the Jeju dog for preservation and protection. A total of 139 dogs from 7 dog breeds, including the Jeju dog, were genotyped using 16 microsatellite markers. The results revealed 2-18 alleles per locus, with a total of 131 alleles among the 16 markers. Most alleles were identified for FH3381, which had 18 alleles, whereas FH2834 had the fewest alleles, with just 2. When the total mean value was observed, the expected heterozygosity and observed heterozygosity were higher for than for outgroup dogs, and the PIC values ranged from 0.000 to 0.862, respectively. The phylogenetic tree analysis of the Jeju dog and other dog varieties revealed that the Jeju dog is closest to the Sapsal dog (0.393). The phylogeny between the Jeju and Korean domestic dogs showed that the Jeju dog is most distant from the Dongkyung dog (0.507). Looking at the distribution individually, the Jeju dog is in the same group as the Labrador Retriever and the Sapsal dog. Meanwhile, the Poongsan, Dongkyung, and Jindo dogs and the German Shepherd were in the same group. Genetic information confirmed through the results of this study can be used as basic data to study the genetic characteristics of the Jeju dog.

• International Journal of Human Ecology
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• v.6 no.2
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• pp.37-49
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• 2005

This paper gives special attention to the changes of time use patterns of the German population for eating and drinking as well as for household work. It especially examines the changes in time use for meals eaten at home and away from home as well as the division of labour for nutrition provision activities between men and women. The presented results originated from two time budget surveys conducted in Germany in 1991/92 and 2001/02. In a secondary analysis time use patterns for eating and drinking and nutrition provision activities were examined on a base of a representative sample of 12600 private households. Surprisingly the amount of time spent on eating and drinking increased over the period. More Germans ate away from home at least once a day, whereas meals at home were still dominant. Moreover comparisons over time revealed that the share in household and food provisioning work has narrowed between men and women, especially in households with both partners being employed. Overall the analysis shows that time use data allow general statements regarding the amount and changes of time spent on daily eating, household work and especially food provision activities. The extent of women's employment has shown to be most decisive for an equal division of household work between gender. To be able to better interpret the results and to understand the consequences for family life, it is necessary to complement time use data with qualitative interviews to gain comprehensive insight into peoples nutrition goals, motives and barriers of action.

• Lingua Humanitatis
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• v.2 no.2
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• pp.5-50
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• 2002

This paper aims to illustrate and illuminate the relationship between language and its neighbor disciplines, in particular between language and literature, language and religion, and language and music. 1. Language and literature. Literature is an art of language. Therefore, linguistics, the science of language, should be able to explain how the grammar of literature elevates and ordinary language into a literary language. I illustrate poetic syntax with examples from Shelley, Coleridge, and Wordsworth. 2. Language and religion. I show how a linguistic analysis of a religious text can illuminate the background, authorship, chronology, etc., of a religious text with an example from the Book of Daniel. I also illustrate how a misanalysis of a poetic meter led to a mistranslation with an example from the Book of Psalms. 3. Language and music. First I trace an epochal event in the history of the Western music, i.e., the change of the musical style from the liturgical music of Latin in which the rhythm was created by the alternation of syllable duration into the liberated music of German in which the rhythm was generated by the alternation of lexical stress. I then illustrate a parallelism between linguistic and musical structures with several musical pieces including Gregorian chant, the 16th century music of Palestrina, the 17th century music of Schutz, the 18th century music of Mozart, and the 19th century Viennese music. Finally, the importance of text-tune (verse-melody) association is discussed with examples of mismatches in translated Korean hymns and contemporary Korean lyrical songs. In the concluding part, I speculate on some factors that are responsible for the same organizational devices in three different modes of human communication. An answer may be that all are under the same laws of mind that govern the way man perceives and organizes nature, i.e., the same cognitive abilities of man, in particular, the capacity to organize and impose structure on their respective inputs.