• 제목/요약/키워드: Genus identification

검색결과 564건 처리시간 0.028초

한국산 흑주름버섯속의 분류학적 연구 (A Taxonomic Study of Genus Melanophyllum in Korea)

  • 석순자;진용주;권순우;김양섭;김완규
    • 한국균학회지
    • /
    • 제41권4호
    • /
    • pp.205-211
    • /
    • 2013
  • 한국산 흑주름버섯속(Melanophyllum)의 종 다양성과 분포상을 조사하는 과정에서 2종이 확인되었고, M. eyrei는 국내 미기록종과 M. haematospermum은 기존 보고된 종이었다. 종 동정은 형태학적 현미경적 특징을 기초로 이루어졌고, 또한 ITS 염기서열 분석을 통해 이루어졌다. 미기록종은 '청흑주름버섯'으로 신칭하고, 기록종인 잔피막흑주름버섯은 종의 특징을 보완하였다.

Microbiological Characteristics of Nocardia takedensis Isolated from Skin Lesion, in Korea

  • Kang, Hye-Sook;Park, Gyu-Nam;Kim, Hye-Ran;Chang, Kyung-Soo
    • 대한의생명과학회지
    • /
    • 제23권2호
    • /
    • pp.96-103
    • /
    • 2017
  • Nocardia species (spp.) are opportunistic pathogen in immunocompromised hosts. The genus Nocardia contains more than 70 species. Nocardia takedensis has been recently reported as a new species of the genus Nocardia. In this study, we describes the first clinical isolate of N. takedensis from the skin lesion in Busan, Korea. For the identification of clinical isolate to the species level as N. takedensis, classical methods (colony morphology, biochemical characteristics, and antimicrobial susceptibility), molecular method (16S rRNA gene sequencing), and MS (mass spectrometry) analysis were conducted. Clinical isolates grew slowly on the culture media (5% sheep blood agar and chocolate agar) under 5% $CO_2$ condition. Especially, carotene pigmentation was detected well on the media. Using mass spectrometry, Nocardia isolate was not identified to the species level. However, molecular method based on 16S rRNA sequencing confirmed the isolate as N. takedensis correctly. N. takedensis isolate was partial positive for acid-fast bacilli on the Ziehl-Neelsen method. And it was observed to be resistance to amoxicillin/clavulanic acid and ciprofloxacin. Our results provide useful information to develop optimal identification protocol of N. takedensis in clinical diagnostic laboratories.

A study on the fine structure of marine diatoms in Korean coastal waters: Genus Thalassiosira 5

  • Park, Joon-Sang;Lee, Jin-Hwan
    • ALGAE
    • /
    • 제25권3호
    • /
    • pp.121-131
    • /
    • 2010
  • Thalassiosira species were collected from October 2007 to January 2009 in an attempt to better understand species diversity of the genus Thalassiosira in Korean coastal waters. A total of 5 Thalassiosira species (T. concaviuscula, T. oceanica, T. partheneia, T. simonsenii and T. nanolineata) were identified here. Most species in this study were of small size, and 5 species were recorded for the first time in Korean coastal waters. Using a scanning electron microscope (SEM), we described distinctive characteristics of fine structure that proved to be important diagnostic characteristics for the identification of each species. The most important diagnostic characteristics for Thalassiosira species identification were the marginal strutted processes, the position of labiate processes, and the areolation. The differential characteristics of the species studied were: T. concaviuscula has a double layered external tubes on the marginal strutted processes; T. oceanica shows marginal ridges that are interlinked between the marginal strutted processes; the valve face of T. partheneia is fairly convex and its labiate process is positioned midway between two strutted processes; T. simonsenii is characterized by two labiate processes and somewhat coarse areolae; and, T. nanolineata has several central strutted processes and linear areolation.

Characterization of Streptomyces sp. AMLK-135 Producing Anti- MRSA Antibiotics

  • Lee, Min-Jeong;Lim, Dae-Seog;Lee, Myung-Sub;Yoon, Won-Ho;Kim, Chang-Han
    • Journal of Microbiology and Biotechnology
    • /
    • 제7권6호
    • /
    • pp.397-401
    • /
    • 1997
  • The present research program was conducted to characterize a strain of actinomycetes producing an anti methicillin-resistant Staphylococcus aureus (MRSA) antibiotic. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among over 400 isolates, a strain (AMLK-135) producing anti-MRSA antibiotic against S. aureus TK 784 was selected. According to the morphological and physiological characteristics, the strain AMLK-135 was confirmed to belong to the genus Streptomyces. From the results of species identification with the TAXON program, the strain AMLK-135 was shown to belong to major cluster 5 (Streptomyces exfoliatus), but it had a low simple matching coefficient ($S_{SM}$ SM/) value to member organisms of major cluster 5. Percentage ($\%$) of strain further away of the strain AMLK-135 was low (1.9400) and it was placed further away than the outer-most members in major cluster 5. Therefore, the strain AMLK-135 was identified as a new species of the genus Streptomyces.

  • PDF

A new approach for identification of the genus Paralia (Bacillariophyta) in Korea based on morphology and morphometric analyses

  • Yun, Suk Min;Lee, Sang Deuk;Park, Joon Sang;Lee, Jin Hwan
    • ALGAE
    • /
    • 제31권1호
    • /
    • pp.1-16
    • /
    • 2016
  • Paralia species have been frequently reported as P. sulcata in Korea, despite the species diversity within the genus. To understand the species diversity of Paralia in Korea, we collected phytoplankton samples at 79 sites from April 2006 to April 2015. Five Paralia species, P. fenestrata, P. guyana, P. marina, P. cf. obscura, and P. sulcata, were observed during this study, and we described their fine structure in terms of quantitative and qualitative morphological characteristics. To provide additional criteria to identify Paralia species more clearly, we morphometrically analysed four quantitative characteristics on valve diameter: pervalvar axis / diameter, internal linking spines / diameter, marginal linking spines / diameter, and fenestrae/diameter using non-metric multidimensional scaling (MDS). MDS analysis distinguished four Paralia species: P. guyana, P. marina, P. cf. obscura, and P. sulcata, with the exception of P. fenestrata. This new approach in using morphometric analysis is useful for the accurate identification of Paralia species.

Taxonomy and distribution of two small Tryblionella (Bacillariophyceae) species from the Northeast Asian tidal flats

  • Im, Ari;Khim, Jong Seong;Park, Jinsoon
    • Journal of Species Research
    • /
    • 제9권3호
    • /
    • pp.191-197
    • /
    • 2020
  • Species belonging to the genus Tryblionella are important members of the diatom assemblages of benthic environments such as tidal flats. Their proper identification is thus of great importance in terms of taxonomy, ecology, as well as environmental sciences. However, many species of the genus have been neglected and/or misidentified during diatom studies of Korean tidal flats possibly due to the small size of the species. Lack of proper references for the identification of the species is also noted. To better understand the diversity of Tryblionella species, sediment samples were taken from various tidal flats of the Yellow Sea. Light microscope (LM) and scanning electron microscope (SEM) observations have shown the presence of Tryblionella adducta and T. hyalina, which have not been previously reported from Korean tidal flats. The former was found mostly from sand flats and the latter from mudflats. It is expected that the present study would contribute to a better understanding of the diversity and ecology of benthic diatoms of the Korean tidal flats.

벼속(Oryza) 식물규소체 검색표와 기재 (Description of the phytoliths of the genus Oryza, with a key to species)

  • 황성수
    • 식물분류학회지
    • /
    • 제39권3호
    • /
    • pp.199-215
    • /
    • 2009
  • 벼속 17 분류군 엽신의 식물규소체에 대해 주사전자현미경의 후반사전자상을 사용하여 조사하고 검색표와 기재를 실시하였다. 검색표는 향축면 및 배축면에 대해 중륵위와 수적세포 규소괴, 유두상돌기, 유혁모, 대모, 미모, 기공장치 등 유래의 식물규소체의 특징에 따라 작성되었으며, 종 수준의 동정이 가능하였다. 본 속 식물의 상세한 규소체 기재와 함께 제공된 후반사전자상 사진 그리고 검색표는 식물의 동정과 고고학적 잔존물에서 출토된 식물규소체를 동정하는데 대조 자료로 사용될 것으로 평가된다.

한국 남해에서 채집된 둥글넙치과(가자미목) 1미기록종, Crossorhombus azureus 후기자어의 형태기재 및 분자동정 (Morphological description and molecular identification of the newly recorded bothid, Crossorhombus azureus (Bothidae, Pleuronectiformes) postlarva collected from the southern sea of Korea)

  • 서민주;유효재;김진구
    • 수산해양기술연구
    • /
    • 제57권3호
    • /
    • pp.264-269
    • /
    • 2021
  • A single postlarva (9.72 mm in standard length) specimen of Crossorhombus azureus (Alcock, 1889) belonging to the family Bothidae, was collected using a bongo net from the southern sea of Korea on December 10, 2019. This species is characterized by having spines on post basipterygial processes. It has two eyes located at the opposite side in head, which accordingly belongs to pre-metamorphosis stage. Melanophores are distributed on the dorsal and anal fin base on the right side (blind side), which is regarded as a useful identification key distinguishing C. azureus from congeneric species in their postlarval stage. A molecular analysis based on mitochondrial DNA COI sequences showed that our specimen was closely matched to adult C. azureus (K2P distance = 0.017). As there is no Korean name for the genus Crossorhombus in spite of presence of Crossorhombus kobensis and its Korean name "Go-be-dung-geul-neob-chi" in Korea, we proposed a new Korean name "Dung-geul-neob-chi-sog" for the genus Crossorhombus and "Pa-lang-dung-geul-neob-chi" for the species C. azureus.

한국 제주도에서 채집된 촉수과(Mullidae) 어류 1첫기록종, Upeneus subvittatus (First Record of Goatfish Upeneus subvittatus (Pisces: Mullidae) from Jeju Island, Korea)

  • 최시원;명세훈;이정훈;김정년
    • 한국수산과학회지
    • /
    • 제57권2호
    • /
    • pp.169-176
    • /
    • 2024
  • On November 9, 2022, a goatfish (Mullidae) that had not been previously reported in Korea was collected during offshore fisheries resources research near Jeju Island. Based on the morphological identification, this goatfish was identified as the genus Upeneus owing to the presence of palatine teeth and vomerine teeth, as well as the proximal part of anterior part of second dorsal fin. Additionally, through molecular identification, the previously unreported goatfish was identified as U. subvittatus with a 99.8% match in the mtDNA COI region. Goatfish U. subvittatus has no patterns on its body and dark bands on both the lower and upper caudal fins, making it well distinguishable from the four species of genus Upeneus reported in Korea. U. vittatus, reported in Japan, showed morphological differences from U. subvittatus in that the dark band on the lower lobe of the caudal fin was wider, and longitudinal stripes were present on the body. Based on the morphological characteristics of U. subvittatus, we suggest a new Korean name, "Jul-mu-nui-kko-li-chog-su".

Detection of Listeria Species by Conventional Culture-Dependent and Alternative Rapid Detection Methods in Retail Ready-to-Eat Foods in Turkey

  • Emine Dincer
    • Journal of Microbiology and Biotechnology
    • /
    • 제34권2호
    • /
    • pp.349-357
    • /
    • 2024
  • Foodborne pathogens, like Listeria monocytogenes, continue to inflict substantial financial losses on the food industry. Various methods for detecting Listeria in food have been developed and numerous studies have been conducted to compare the different methods. But, in recent years, new Listeria species have been identified, and currently the genus comprises 26 species. Therefore, it would be a more accurate approach to re-evaluate existing detection methods by considering new species. The present investigation involved the analysis of 42 ready-to-eat (RTE) foods, encompassing a variety of food categories, such as mezes, salads, dairy products, and meat products, with the aim of ascertaining the presence of Listeria. Among the traditional culture-dependent reference methods, the ISO 11290 method was preferred. The process of strain identification was conducted with the API Identification System. Furthermore, to ascertain the existence of L. monocytogenes and Listeria spp., the samples underwent additional analysis employing the VIDAS Immunoassay System, ELISA, and RT-PCR methodologies. Thus, four alternative approaches were employed in this study to compare not only the different methods used to determine Listeria while taking into account the newly identified Listeria species, but also to assess the compliance of retail RTE food items with microbiological criteria pertaining to the genus Listeria. Based on the conducted analyses, L. monocytogenes was conclusively determined to be present in one sample. The presence of Listeria spp. was detected in 30.9% of the samples, specifically in Turkish cig kofte, sliced salami, and salads.