• Korean Journal of Environment and Ecology
• /
• v.16 no.4
• /
• pp.387-403
• /
• 2003

The vascular plants in Manbokdae-Yeowonjae section of the Baekdudaegan were consisted of 502 taxa; 104 families,305 genus,439 species, 60 varieties, 3 forma. From the floristic point of view, the Baekdudaegan of Manbokdae-Yeowonjae section belongs to the southern province in Korea. The rare and endangered plants designated by Korea Forest Service were Abies koreana, Pedicularis verticillata, Patrinia saniculaefolia, Hololeion maximowiczii, Lilium callolsum, Paeonia obovate, Tricyrtis dizutata and VioEa diamantica, and Cymbidium goeringii. The Korean endemic species were Abies koreana, Patrinia saniculaefolia, Vicia unijuga, Stewartia koreana, Cornus walteri, Weigela subsessilis, Saussurea seoulensis, Tyicyrtis dilatata, Hosta capitata, Clematis trichotoma et at.

• The Plant Pathology Journal
• /
• v.18 no.2
• /
• pp.85-92
• /
• 2002

A new isolate of Cucumber mosaic virus (CMV), identified as Li-CMV was isolated from a diseased Korean native lily (Lithium tsingtauense Gilg). Biological and serological properties of Li-CMV were characterized, and reverse transcription-polymerase chain reaction (RT-PCR) analysis, restriction enzyme profiling of RT-PCR products, and nucleotide sequence analysis of RNA3 of the virus were performed in this study. Remarkable differences in symptoms between Li-CMV and ordinary CMV strains were found in tobacco plants and Datura stramonium. Li-CMV-infected tobacco plants (cv. Xanthi-nc and cv. Samsun) induced chlorotic ringspots on uninoculated upper leaves, and the symptom expression was delayed or faint whereas, ordinary CMV strains induced green mosaic symptoms on the plant. Systemic infections were observed on Nicotiana benthamiana with severe mosaic symptom. Restriction mapping analysis of RT-PCR products using MspI showed that Li-CMV belonged to CMV subgroup I. A full-length CDNA copy of RNA3 for the virus was amplified by RT-PCR, cloned, and its complete nucleotide sequence was determined. The RNA3 of Li-CMV was 2, 232 nucleotides long, and consisted of two open reading frames of 843 and 657 bases encoding 3a protein (movement protein) and coat protein, respectively. Results of this study indicate that Li-CMV is a novel strain and belongs to subgroup I of CMV in the genus Cucumovirus.

• The Plant Pathology Journal
• /
• v.16 no.4
• /
• pp.231-235
• /
• 2000

The 3,000 nucleotides of 3'-terminal region of the genomic RNA of a new isolate of carlavirus from a Korean native lily (Lilum lancitoium) was cloned and its nucleotide sequences were determined. The coat protein (CP) gene of the virus showed 72.0% to 72.8% nucleotide sequence identities and 86.9% to 88.0% amino acid sequence identities with those of the four strains (two Korean, one Dutch, and one Japanese isolates) of lily symptomless virus (LSV). Interestingly, different amino acid sequences between the new isolate and LSV strains were located at the N-terminal region of the CP. Pairwise amino acid sequence comparison of the CP gene revealed sequence identities of 22.0% to 71.1% between the virus and other 9 carlavirus species. The 25 kDa and 12 kDa proteins genes of the virus share 30.7% to 76.3% and 31.1% to 85.8% amino acid sequence identities, respectively, with those of 8 other carlaviruses. The 16 kDa protein gene of the virus shares 16.7% to 72.9% amino acid sequence identities with that of 9 other carlaviruses. These data indicate that the virus, designated as lily latent virus (LiLV), is a distinct of the Carlavirus genus and distinguished from the known strains of LSV.

• Korean Journal of Environment and Ecology
• /
• v.18 no.2
• /
• pp.221-230
• /
• 2004

Flora of the Dokdo Islet, where located south-east from Ulnung Island, off the east coast of the Korean peninsula, recorded as 59 taxa; 29 families, 50 genus, 48 species, 1 sub-species, 9 varieties and 1 form. Dong-do (East islet) of Dokdo Islet, naturalized plants such as Brassica campestris subsp. napus var. nipp-oleitera and Rumex crispus are widely ranged over the islet. The naturalizedplants affects the native species and their habitats of Artemisia japonica var. macrocephala, Echinochloa crus-galli, Sedum oryzifolium, Arabis stelleri var. japonica and Sedum kamtschaticum var takesimense in the islet. The trail in Seo-do (West islet) is heavily threatened by over-all weathering processes on the rock. The native woody species in the islet are Euonymous japonicus and Lonicera insularis, and the species such as Pinus thunbergii and Hibiscus syriacus are planted in the recent past under the IRP (Islet Restoration Program) by NGO. The Specific Plants, which is designated and categorized by the Ministry of Environment, Korea, such as Lilium lancifolium, Lysimachia mauritiana and Reynoutria sachalinensis are ranged. The ecosystem of Dokdo islet is seriously threatened by invadedplant species and building works for garrison and relevant facilities. The over-all long- and short-term strategies, based on interdisciplinary and integrated approaches for habitat restoration is needed.

• Korean Journal of Environment and Ecology
• /
• v.29 no.6
• /
• pp.819-841
• /
• 2015

To investigate the distribution of vascular plants growing at Halmibong(1,026m) and Gusibong(1,014m) in Baekdutrail, we surveyed from March in 2013 to March in 2014. The flora of Haimibong and Gusibong in Baekdu trail were found to be; 528 taxa; 95 families, 287 genus, 454 species, 4 subspecies, 59 varieties, 11 forma. Rare plants were found to be; 15 taxa, 10 families, 14 genus, 13 species, 2 varieties. Among them EN degree was found to be 1 species(Rhododendron tschonoskii Maxim.), VU degree; 2 species(Scorzonera albicaulis Bunge, Lilium cernuum Kom.), LC degree; 12 species(Abies koreana Wilson, Coreanomecon hylomeconoides Nakai, Viola albida Palib. etc.) respectively. In all surveyed areas, a total of 15 taxa(Heloniopsis koreana Fuse, N.S.Lee & M.N. Tamura etc.) were found to be endemic to Korea. Distribution of floristic special plants in the surveyed areas were found to be divided into five classes (class I~V). The floristic special plants found in surveyed areas were 2 taxa of grade V, 6 taxa of grade IV, 12 taxa of grade III, respectively. The naturalized plants were investigated as 23 taxa; Rumex crispus L., Amorpha fruticosa L., Veronica arvensis L. etc..

• Horticultural Science & Technology
• /
• v.33 no.6
• /
• pp.891-899
• /
• 2015

The lily symptomless virus (LSV) is the most common virus in Korean native lilies and causes various types of damage to overall plant growth. This study was carried out to investigate the elimination rate of the LSV by the in vitro scale culture (scaling) method in Korean native lilies and to test reinfection rates of the LSV under several field culture conditions of bulb production. Four Korean native lilies (Lilium dauricum, L. distichum, L. lancifolium, and L. maximowitzii) were used and their scales were cultured in vitro for micro-scale formation. The micro-scales were subcultured repeatedly using MS culture medium supplemented with 30 or $90g{\cdot}L^{-1}$ sucrose. The culture conditions were $24{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with 16 hour daylength using fluorescent lamps and maintained at $22{\pm}2^{\circ}C$. The virus-free bulblets were grown for one to three years in the greenhouse and transplanted to the field in October or March. Virus infection rates were investigated by direct tissue blotting immunobinding assays and measurement of chlorophyll and protein contents. Virus-free plants could be obtained from the 5th subculture of micro-scales in L. lancifolium and L. maximowitzii or from primary culture in L. dauricum and L. distichum. LSV-free plants were reinfected during bulb production in the field. Reinfection rates were higher at older bulb ages and under higher planting density. The plants planted in October and at inland Gyeongsan had higher infection rates than those planted in March and at coastal area Pohang. The reinfection rate of L. maximowitzii was higher than those of L. dauricum and L. lancifolium. The LSV-infected plants had lower chlorophyll contents and unchanged protein contents compared to virus-free plants.