• 한국임상수의학회지
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• 제21권2호
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• pp.133-139
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• 2004

Bacterial pathogens were isolated from 36 dogs with respiratory signs, that were submitted to Veterinary Clinics in Jeju, including Veterinary Medical Teaching Hospital in Cheju National University. Of 36 isolates, 16 (44.4%) bacterial pathogens were Gram-positive and 20 (55.6%) were Gram-negative. Gram-positive bacteria identified with API Staph were 12 S. intermedius (33.3%), 2 S. aureus (5.6%), 1 S. haemolyticum (2.8%), and 1 S. xylosus (2.8%). Gram-negative organisms identified with API 20E or API NE included 8 Bordetella bronchiseptica (22.2%), 6 Escherichia coli (16.7%), 4 Pasteurella spp. (11.1%), 1 Enterobacter intermedius (2.8%), and 1 Oligella ureolytica (2.8%). Both Staphylococcus spp. isolates and Gram-negative pathogens were resistant to one or more antibiotics, including ampicillin (AM), amoxicillin/clavulanic acid (AMC), chloramphenicol (C), cefazolin (CZ), erythromycin (E), gentamicin (GM), kanamycin (K), lincomycin (L), oxacillin (OX), trimethoprim/sulfamethoxazole (SXT), and tetracycline (TE). All Staphylococcus spp. were susceptible to AMC, OX and VA, while many isolates were highly resistant to L (87.5%), E (68.8%), P (62.5%), and AM (56.3%). Antibiotic-resistant patterns of staphylococcal isolates were shown ranges from single to 9-resistant patterns. Resistant rates to antibiotics of Gram-negative bacteria were usually higher than those of Staphylococcus spp. in this study. Most Gram-negative bacteria were highly resistant to L (90.0%), AM (85.0%), E (85.0%), P (85.0%), OX (80.0%), and CZ (75.0%). B. bronchiseptica isolates showed 5 to 8 antibiotics-resistant patterns and Pasteurella spp., 2 to 8-resistant patterns. In particular, all 6 E. coli isolates were resistant to more than 9 different kinds of antibiotics, including one strain resistant to all antibiotics tested.

• 대한수의학회지
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• 제29권3호
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• pp.315-324
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• 1989

In order to establish the extent of Streptococcal arthritis piglets, isolation of Streptococci from arthritic lesions of 34 piglets were undertaken from November 1987 to October 1988 in Korea. Also determined were isolation frequency of Streptococci in nasal cavity of 250 healthy sows and antibiotic susceptibilities of the isolates. Streptococci were isolated from 52.9% of 34 arthritic piglets and 20 strains isolated belonged to 4 S suis type I, 8 S suis type II, 2 Lancefield group C and 6 group E. From 28.8% of 250 healthy sows, 72 strains of Streptococci were isolated and these consisted of 9 S suis type I, 51 S suis type II and 12 group C. Streptococcal arthritis seemed to occur prominently in piglets aged 2 to 4 weeks and in male than female. No significant difference were recognized in tarsal and carpal joints as affecting site. All of 92 isolates were sensitive to ampicillin and penicillin, and all strains of S suis type I and group E Streptococcus were also sensitive to chloramphenicol and cephalothin. To cephalothin all strains of group C Streptococcus were sensitive. The 1. 7 to 100% of 92 isolates were resistant with different prevalence to colistin, erythromycin, kanamycin, tetracycline, gentamicin, chloramphenicol and cephalothin. The 92.5% of these resistant Streptococci were multiply drug-resistant strains. The drug resistant patterns most frequently encountered were Tc Cl Em Km Gm(16.3%) in quintuple pattern, Tc Cl Em Km(16.3%) in quadruple pattern, Tc Cl Em(10.9%) in triple pattern and Cl Em(14.1%) in double pattern.

• 대한미생물학회지
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• 제35권3호
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• pp.239-250
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• 2000

Sixty-eight clinical isolates of Stenotrophomonas maltophilia from inpatients of 2 university hospitals in Taegu were epidemiologically analyzed by using the minimum inhibitory concentrations of 25 antimicrobial drugs, biochemical reaction, pulsed-field gel elctropgoresis (PFGE), and PCR with enterobacterial repetitive intergenic consensus sequences as primer (ERIC-PCR). 1. All the strains were susceptible to minocycline. More than 57% were susceptible to sulfisomidine (Su), ciprofloxacin (Ci), Ofloploxacin (Of), nalidixic acid (Na), and chloramphenicol (Cm), and $19{\sim}35%$ to ceftazidime (Cd), trimethoprim (Tp), Ticacillin-clavulanic acid, and cefoperazone-sulbactam. Most isolates were resistant to ${\beta}$-lactam antibiotics such as ampicillin (Ap), carbenicillin (Cb), cefotaxim (Ct), cefoxitin (Cx), and aminoglycosides including gentamicin (Gm), tobramycin (Tb), amikacin (Ak). 2. All the isolates were multiply resistant of 5 to 17 drugs and showed 40 different resistance pattern types. 3. All the strains showed very similar biochemical reactions except ${\beta}$-galactosidase and nitrate reduction test. Fourteen strains selected randomly were classified 10 different pattern type by PFGE and ERIC-PCR. These two methods showed identical result. Four strains isolated from wound in 1994 showed similar MIC pattern and identical API 20NE profile, PFGE, and ERIC-PCR pattern indicating episodes of cross-infection among patients. These results indicate that PFGE or ERIC-PCR profile has comparable discriminatory power for epidemiological typing of S. maltophilia.

• Current Research on Agriculture and Life Sciences
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• 제4권
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• pp.132-140
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• 1986

시판(市販) 사료첨가제(飼料添加劑)에 대한 미생물학적(微生物學的) 오염정도(汚染程度)를 알아보기 위하여 국내(國內)에서 판매(販賣)되고 있는 비타민과 횡물질(鐄物質) 사료첨가제(飼料添加劑) 36개(個) 품목(品目) 총(總) 81례(例)의 시료(試料)를 시험(供試)하여 일반세균(一般細菌) 및 대장균군(大腸菌群)의 오염상태(汚染狀態)를 검사(檢査)하고 아울러 시료(試料)로부터 분리(分離)한 83주(株)의 대장균군(大腸菌群)에 대한 Am등(等) 8종(種)의 항균성낙제(抗菌性樂劑)에 대한 내성빈도(耐性頻度) 및 내성정도(耐性程道)를 파악하였으며 내성균(耐性菌)에 있어서는 내성양상(耐性樣相)과 R plasmid의 분포(分布)를 조사(調査)하여 다음과 같은 성적(成績)을 얻었다. 일반세균(一般細菌)은 시료(試料) 81례(例) 모두 양성(陽性)이었으며 대장균군(大腸菌群)은 81례중(例中) 14례(例)(17.3%) 에서만 양성(陽性)이었다. 일반세균수(一般細菌數)의 분포(分布)는 g당(當) 10미만에서부터 1,400,000까지 다양(多樣)하였으며 그 중(中) 100~1,000/g이 34례(例)(42%)로 가장 많았고 대장균군(大腸菌群)에 있어서는 일반세균(一般細菌)의 오염도(汚染度)가 높을수록 분리율(分離率)이 높았으며 총(總) 18개(個) 제조회사중(製造會社中) 6개사(個社)(33.3%)의 제품(製品)에서 양성(陽性)이었다. 공시균(供試菌) 83주중(株中) 41주(株)(49.4%)가 fecal coliform이었다. 공시균(供試菌)에 대한 약제별(藥劑別) 내성균출현율(耐性菌出現率)은 sulfadimethoxine (Sa)에 대해 92.8%로 가장 높았고 다음으로 streptomycin (Sm)에 67.5%, tetracycline (Tc)에 50.6%, kanamycin (Km)에 26.5%, chloramphenicol(Cm)에 18.1%, ampicillin(Am)에 15.7% 순(順)이었으며 nalidixic acid(Na)와 gentamicin (Gm)에는 전주(全株)가 감수성(感受性)이었고 각공시(各供試) 약제(藥劑)에 있어서 non-fecal coliform에 비하여 fecal coliform의 내성균출현율(耐性菌出現率)이 높았다. 공시균(供試菌)의 최소발육저지농도(最小發育沮止濃度)(minimum inhibitory concentration, MIC) 분포(分布)는 Am 및 Km에 대하여 MIC가 $3,200{\mu}g/m{\ell}$ 이상(以上)인 고도(高度)의 내성(耐性)을 가진 균(菌)이 각각(各各) 7주(株) 및 3주(株)이었으나 대부분의 내성균(耐性菌)은 그 MIC가 $25{\mu}g/m{\ell}$이었고, Cm, Sm 및 Tc에 대한 내성균(耐性菌)의 대부분은 $25{\mu}g/m{\ell}$에서 $400{\mu}g/m{\ell}$의 범위(範圍)이었다. 공시균(供試菌) 83주중(株中) 79주(株)(95.2%)가 공시(供試)한 약제(藥劑) 1종(種) 이상(以上)에 내성(耐性)을 가졌으며 내성형별(耐性型別)로는 SaSm 내성형(耐性型) 및 Sa 단제내성형(單劑耐性型)이 각각(各各) 12주(株)(14.5%)로 가장 많았고 다음으로 SaSmTc형(型) 10주(株)(12%), SaSmTcKm형(型) 7주(株)(8.4%), SaTc형(型) 7주(株)(8.4%) 및 SaSmKm형(型) 6주(株)(7.2%)의 순(順)이었으며 총(總) 19종(種)의 내성형(耐性型)이 관찰되었다. 내성전달시험(耐性傳達試驗) 결과(結果) 내성균(耐性菌) 79주중(株中) 32주(株)(40.5%)가 전달성(傳達性) R plasmid를 보유(保有)하고 있었으며 다제내성균(多劑耐性菌)일수록 내성전달률(耐性傳達率)이 높았다. 공시(供試) 약제별(藥劑別) 내성전달빈도(耐性傳達頻度)는 Am (100%) 및 Cm (80%)에서 매우 높고, 다음으로 Tc (38.1%), Sa (18.2%), Sm (17.9%) 및 Km (4.5%)의 순(順)이었다.

• 대한미생물학회지
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• 제13권1호
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• pp.7-16
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• 1978

환자(患者)로부터 분리(分離)한 103주(株)의 장구균(腸球菌)을 대상(對象)으로 항생제감수성검사(抗生劑感受性檢査)와 더불어 R plasmid에 의(依)한 약제내성전달(藥劑耐性傳達)을 실험(實驗)하였다. 전(全) 균주(菌株)에 대(對)한 MIC는 ampicillin이 $4{\mu}g/ml$로 가장 낮았으며 rifampin이 $16{\mu}g/ml$, amikacin과 chloramphenicol이 공(共)히 $125{\mu}g/ml$, tetracyclin이 $250{\mu}g/ml$이었고 분리균주(分離菌株) 중(中) $2,000{\mu}g/ml$ 고농도(高濃度)의 GM, KM 및 SM에 내성(耐性)을 보인 균주(菌株)는 각각(各各) 19주(株)(18.5.%), 27주(株)(26.2%) 그리고 22주(株)(21.4%)이었다. 가검물별(可檢物別) 분리균주(分離菌株)의 약제내성도(藥劑耐性度)는 뇨(尿)에서 분리(分離)된 균(菌)의 내성도(耐性度)가 가장 높았으며 각(各) 항생제(抗生劑)에 대(對)한 균종(菌種)의 약제내성도(藥劑耐性度)는 S. zymogenes의 내성도(耐性度)가 ampicillin과 rifampin을 제외(除外)한 기타(其他) 약제(藥劑)에 대(對)해 가장 높았다. 다제약제내성(多劑藥劑耐性)을 보인 28주중(株中) 17주(株)(61%)가 내성유형(耐性類型)의 일부(一部) 또는 전부(全部)를 전달(傳達)하였으며 이 중(中) 2주(株)만이 그 유형(類型)의 전부(全部)를 전달(傳達)하였고 내성전달빈도(耐性傳達頻度)는 $2{\times}10^{-4}-2{\times}10^{-6}%$이었다. 이상(以上)의 실험결과(實驗結果) 저자(著者)들은 우리나라에서 분리(分離)된 장구균(腸球菌)이 수종(數種)의 항생제(抗生劑)에 내성(耐性)이며 장구균(腸球菌)에 있어서도 약제내성전달(藥劑耐性傳達)이 R plasmid에 의(依)해 이루어짐을 결론(結論)지을 수 있었다.

• 한국동물위생학회지
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• 제24권1호
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• pp.31-41
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• 2001

This study was carried out to identify causative agents from california mastitis test(CMT) positive mastitic milk, and to examine the antimicrobial susceptibility of 50 heads in Seongju and Chilgok area of Gyeongbuk province. Sulfadimethoxine sodium(SMS) was intramuscularly administered once to four mastitis Infected dairy cattle at the rate of 10mg/kg body weight. After injection of SMS, the depletion rate of serum and milk from the cattle were periodically measured for five days. The CMT positive number from 50 heads showed 46% and that of 200 quarters was 47(23.5%). From 39 quarters of 47 heads 39 different microorganisms were identified. These organisms were classified into 12 species : Staphylococcus aureus 8(20.5%), Sta hemolyticus 6(15.4%), Streptococcus bovis 4(10.3%), Sta hyicus 3(7.7%), Sta epidemidis. Sta xylosus, Sta sciuri 2(5.1%), Str agalactiae 2(5.1%), Escherichia coli(10.3%), three Enterobacter cloacae(7.7%), two Ent aerogenes(5.1%) and one Salmonella spp(2.6%). As the results of antibiotic susceptibility test, gentamicin(Gm, 11 species 27 strains, 69.5%), cephalotin(Cf, 9 species 24 strains, 61.5%), sulfamethoxazole(Stx, 8 species 14 strains, 43.6%), tetracycline(Tc, 8 species 14 strains, 35.9%), and streptomycin(Sm), lincomycin(Lm), cefoperazon(Cp) and penicillin(Pc) have a highly resistance(7.7% ～5.1%). However, carbenicillin (Cb), amikacin(Ah) have no susceptible for all drugs. The mean residual concentration of SMS in serum according to the time lapsed were showed 33.964 $\pm$ 4.435ppm at the 4 hours after intramuscularly injection(AII). It was significantly(p<.05) decreased to 6.596 $\pm$ 3.402, 0.217 $\pm$ 0.119 and 0.005 $\pm$ 0.004ppm at the 1st, 3rd and 5th day AII. The mean concentration of SMS in milk was significantly(p<.05) decreased from 0.920 $\pm$ 0.42ppm to 0.084 $\pm$ 0.016ppm between 8 hours and 1 day AII. As the results of this experiments, sulfadimethoxine was residued at the level of no less than 0.01ppm in milk on the 2nd day AII. Thus, this results would be able to be used the basic index for prevention of sulfonamides residue in milk after treatment of dairy mastitis.

• 대한미생물학회지
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• 제19권1호
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• pp.11-24
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• 1984

Shigella remains to be an important enteric pathogen in this country for the present. Moreover, most of the isolates have become multiple resistant to various antibiotics which used to be drugs of choice for shigellosis. This study was made as an attempt to assess the present stage of antibiotic resistance and the incidence and transferability of R factors of Shigella. A total of one hundred and seventeen strains of Shigella isolated from patients in Seoul and provincial area between 1982 and 1983 were tested for their resistant to antimicrobial agents and transmission of R-plasmid. Antibiotic susceptibilities were determined by an agar dilution method. Muller hinton agar were used for the assay of drug resistance and tryptic soy broth were used for propagating medium for conjugation. Shigella isolated found to be one or more antibiotics were considered potential donor of R-plasmid. The following results were obtained. 1. Among 117 strains of Shigella isolated, 111 strains(94.9%) were found to be resistant to one or more drugs tested and 97.3% of these resistant strains were multiply resistant, indicating the multiply resistant strains were more than the single resistant strains. Only six strains were susceptible to all drugs tested. 2. Among 117 strains of Shigella isolated, 107 strains(91.5%) were resistant to Tetracyclin(Tc), 106 strains(90.6%) to Chloramphenicol(Cp) and Streptomycin(Sm), 97 strains(82.9%) to Ampicillin(Ap), 68 strains(58.1%) to Cephaloridine(Cr), 10 strains(8.5%) to Nalidixic acid(Na), 5 strains(4.3%) to Kanamycin(Km) and 2 strains(1.7%) to Rifampicin. No strain was resisfant to Amikacin(Ak) and Gentamicin(Gm). 3. All drug-resistant Shigella strains, except three, were multiply resistant to two or more drugs. Fifty eight strains were resistant to five drugs, followed by 26 strains resistant to dour drugs, 12 strains resistant to three drugs and 11 strains resistant to six drugs. 4. The 73% of multiply drug-resistant Shigella transferred their resistance to E. coli by conjugation and the resistance was considered to be mediated by R-plasmid. Resistance to Nalidixic acid and Rifampicin were not transferred by conjugation to recipient. As for the transferability of resistance to each seperate drug, Ap resistance was transferred with 73.2% frequence and Cm and Tc resistance were transferred with approximately 50-60% frequence whereas Sm and Cr resistance were transferred in 19.1-21.4% The other four drugs resistant failed to transfer their resistance to recipient. 5. As for the incidence and transferability of resistance to each seperate drug, the strains resistant to Tc and Cm were encountered most frequently with the rate of 91-92%, whereas transfer of Tc and Cm were low, 51-52%. The incidence of Sm resistance was very high(90.6%) but transferability of drugs resistance was much lower(25.4%). Though the incidence of Km reristance was much lower(4.3%) transferability of Km resistance was considerably higher(60%). 6. The greater the multiplicity of resistance, the greater was the likelihood that part of all of the resistance markers would be transferable.