The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Antimicrobial Resistance and Molecular Epidemiologic Characteristics of Stenotrophomonas maltophilia Isolated from Clinical Specimens

병원 재료에서 분리한 Stenotrophomonas maltophilia의 항균제 내성 및 분자역학적 특성

  • Seol, Sung-Yong (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Jang, Kyoung-Soo (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Jeong, Oung-Gi (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Cho, Eung-Rae (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Kim, Neung-Hee (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Yu, Hak-Sun (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Lee, Yoo-Chul (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Cho, Dong-Taek (Department of Microbiology, School of Medicine, Kyungpook National University)
  • 설성용 (경북대학교 의과대학 미생물학교실) ;
  • 장경수 (경북대학교 의과대학 미생물학교실) ;
  • 정웅기 (경북대학교 의과대학 미생물학교실) ;
  • 조응래 (경북대학교 의과대학 미생물학교실) ;
  • 김능희 (경북대학교 의과대학 미생물학교실) ;
  • 유학선 (경북대학교 의과대학 미생물학교실) ;
  • 이유철 (경북대학교 의과대학 미생물학교실) ;
  • 조동택 (경북대학교 의과대학 미생물학교실)
  • Published : 2000.06.30
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Abstract

Sixty-eight clinical isolates of Stenotrophomonas maltophilia from inpatients of 2 university hospitals in Taegu were epidemiologically analyzed by using the minimum inhibitory concentrations of 25 antimicrobial drugs, biochemical reaction, pulsed-field gel elctropgoresis (PFGE), and PCR with enterobacterial repetitive intergenic consensus sequences as primer (ERIC-PCR). 1. All the strains were susceptible to minocycline. More than 57% were susceptible to sulfisomidine (Su), ciprofloxacin (Ci), Ofloploxacin (Of), nalidixic acid (Na), and chloramphenicol (Cm), and $19{\sim}35%$ to ceftazidime (Cd), trimethoprim (Tp), Ticacillin-clavulanic acid, and cefoperazone-sulbactam. Most isolates were resistant to ${\beta}$-lactam antibiotics such as ampicillin (Ap), carbenicillin (Cb), cefotaxim (Ct), cefoxitin (Cx), and aminoglycosides including gentamicin (Gm), tobramycin (Tb), amikacin (Ak). 2. All the isolates were multiply resistant of 5 to 17 drugs and showed 40 different resistance pattern types. 3. All the strains showed very similar biochemical reactions except ${\beta}$-galactosidase and nitrate reduction test. Fourteen strains selected randomly were classified 10 different pattern type by PFGE and ERIC-PCR. These two methods showed identical result. Four strains isolated from wound in 1994 showed similar MIC pattern and identical API 20NE profile, PFGE, and ERIC-PCR pattern indicating episodes of cross-infection among patients. These results indicate that PFGE or ERIC-PCR profile has comparable discriminatory power for epidemiological typing of S. maltophilia.

Keywords

References

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