• Journal of Animal Science and Technology
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• 제49권5호
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• pp.577-584
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• 2007

본 연구는 후대검정 한우 295두의 혈액으로부터 genomic DNA를 추출하여 PCR 방법에 의한 증폭과 염기서열 분석을 통하여 ADSF/resistin 유전자의 단일염기다형을 발굴하고 이들과 한우 육질관련형질과의 상관관계를 분석하기 위하여 실시하였다. 확보된 DNA로부터 염기서열을 결정한 결과 promoter와 4개의 exon영역에서는 SNP를 찾지 못하였으나 intron 영역에서 7개의 SNP를 발굴하였다. 발굴된 SNP의 출현 빈도는 0.027에서 0.16까지 그 차이가 많았다. 육질형질과의 상관분석에서 이들 SNP 중 intron 2에서 발굴된 764A ins 만이 근내지방도와 상관관계가 발견되었다(P<0.05). 근내지방도는 유전력이 매우 높기 때문에 이번에 발굴된 ADSF/resistin 유전자의 SNP 764A ins와 같이 유전표지인자를 이용하는 것이 근내 지방도의 개량을 위해 우수한 결과를 가지는 것으로 사료된다. 가축의 경제형질의 경우 다수의 유전자가 관여하기 때문에 한 개의 유전자를 이용한 가축의 선발 또는 개량에 이용한다는 것은 제한적일 수 있다. 따라서 다수의 관련 유전자를 이용한 다형현상과 경제형질과의 연관성 연구가 동반될 때 육질개선 및 가축개량에 실질적인 증대효과가 있을 것으로 사료된다.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.135-146
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• 2011

The objective of the present research was to map QTLs associated with agronomic traits such as days from sowing to flowering, plant height, yield and leaf-related traits in a population of recombinant inbred lines (RILs) of sunflower (Helianthus annuus). Two field experiments were conducted with well-irrigated and partially irrigated conditions in randomized complete block design with three replications. A map with 304 AFLP and 191 SSR markers with a mean density of 1 marker per 3.7 cM was used to identify QTLs related to the studied traits. The difference among RILs was significant for all studied traits in both conditions. Three to seven QTLs were found for each studied trait in both conditions. The percentage of phenotypic variance ($R^2$) explained by QTLs ranged from 4 to 49%. Three to six QTLs were found for each yield-related trait in both conditions. The most important QTL for grain yield per plant on linkage group 13 (GYP-P-13-1) under partial-irrigated condition controls 49% of phenotypic variance ($R^2$). The most important QTL for 1,000-grain weight (TGW-P-11-1) was identified on linkage group 11. Favorable alleles for this QTL come from RHA266. The major QTL for days from sowing to flowering (DSF-P-14-1) were observed on linkage group 14 and explained 38% of the phenotypic variance. The positive alleles for this QTL come from RHA266. The major QTL for HD (HD-P-13-1) was also identified on linkage group 13 and explained 37% of the phenotypic variance. Both parents (PAC2 and RHA266) contributed to QTLs controlling leaf-related traits in both conditions. Common QTL for leaf area at flowering (LAF-P-12-1, LAF-W-12-1) was detected in linkage group 12. The results emphasise the importance of the role of linkage groups 2, 10 and 13 for studied traits. Genomic regions on the linkage groups 9 and 12 are specific for QTLs of leaf-related traits in sunflower.

• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.9-19
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• 2016

Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37℃ and could maintain at least 96% activity after being placed at 37℃ for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, K_m, V_max, and k_cat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.

• 한국균학회지
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• 제42권1호
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• pp.1-8
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• 2014

본 연구에서는 국내외에서 수집한 A. bisporus 45계통과 Agaricus spp. 19균주를 포함한 64균주로부터 genomic DNA를 추출하고 12종류의 UFPF primer (JK Biotech., Ltd.)를 사용하여 PCR 다형성 분석을 실시한 바 양송이 계통간 PCR다양성이 관찰되었다. 7종류의 UFPF primer에 의해 생성된 A. bisporus계통의 95 PCR다형성 밴드가 유전적 유사도 산출에 이용되어 UPGMA cluster분석을 적용 dendrogram을 작성한 결과 A. bisporus의 계통군은 3 cluster 내에 8개의 세부 group으로 분류되었으며 유사성 함수가 0.75에서 0.90의 유연관계를 보였다. 한국에서 최근 개발된 새아, 새정, 새연, 새도는 같은 group에 포함되었으며 0.96%에서 1.0%의 밀접한 근연관계에 있었으며 그밖에 한국품종은 미국 유럽의 양송이 품종과 유전적 유연관계를 보였다.

• 생명과학회지
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• 제21권10호
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• pp.1370-1376
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• 2011

고세균 Sulfolobus acidocaldarius의 기능유전체학 연구를 위하여 피리미딘 생합성 유전자군의 pyrEF 유전자에 근거한 피리미딘 영양요구주를 구축하였다. 원균주는 정상적인 pyrEF 존재하에서 5-fluoroorotic acid를 첨가하면 성장이 불가능하나 피리미딘 영양요구주는 성장이 가능한 원리를 활용하였다. 자외선을 이용하여 얻어진 5-FOA 첨가에 저항성을 갖는 돌연변이주를 얻었으며, 두 돌연변이주 KH1U와 KH2U는 각각 pyrE 유전자 부분의 점돌연변이와 삽입돌연변이를 갖는 돌연변이주임을 알 수 있었다. 이 두 돌연변이 균주는 5-FOA의 첨가에 의하여 이 세포를 사멸시킬 수 있는 능력이 사라짐을 확인하였다. 정상적인 pyrEF 유전자를 갖는 Sulfolobus-E. coli 플라스미드를 이용하여 보완실험을 수행한 결과 KH2U 돌연변이주는 다시 5-FOA에 대한 저항성을 잃어버렸으며, 배지내에 피리미딘의 첨가가 없어도 생존할 수 있는 능력을 보여주는 원균주와 같은 표현형으로 회귀함을 확인하였다. 이 연구는 차후 고세균 Sulfolobus acidocaldarius의 유전자 불활성화를 통한 유전학연구에 효율적인 도구로 사용되기에 유용한 연구로 생각된다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제4권2호
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• pp.213-217
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• 2001

목적: 당원병 Ia형(von Gierke disease)은 glucose 6-phosphatase (G6Pase)의 결함으로 인하여 출생 시부터 복부팽만과 간종대가 나타나고 저혈당, 락트 산혈증(lactic acidemia), 고지방혈증(hyperlipidemia), 고뇨산혈증(hyperuricemia) 등을 초래하며 점차 성장발육부전이 진행되는 상염색체 열성 유전성 질환이다. 본 연구에서는 한국인 GSD Ia 환자 9명을 대상으로 G6Pase 유전자의 돌연변이형에 관한 분석을 처음으로 시행하고자 하였다. 방법: 임상적인 증상과 G6Pase 효소 측정결과를 통하여 GSD Ia로 진단된 9명 환자의 혈액에서 genomic DNA를 분리하여 G6Pase 유전자의 5개 exon 부분이 포함되도록 polymerase chain reaction(PCR) 한 후 PCR 산물을 direct sequencing 하였다. 결과: 9명의 GSD Ia 환자 중 7명 환자에서 g727t homozygote 돌연변이를 발견하였고, 한 환자는 heterozygote로서 g727t 돌연변이와 c611g 돌연변이가 발견되었다. 나머지 한 명에서는 g727t 돌연변이 하나만 발견할 수 있었다. 한 개의 allele에서 발견된 c611g 돌연변이는 exon 4의 178번 아미노산 proline이 alanine으로 변경되는 것으로 지금까지 보고된 바 없는 새로운 돌연변이이다. 결론: 현재까지는 GSD Ia병의 정확한 진단을 위해서 환자의 간 생검 조직에서 G6Pase 효소의 활성도를 측정하는 것이 필요하였으나 한국인 GSD Ia 환자의 경우 g727t 돌연변이가 대부분을 차지하고 있기 때문에 앞으로는 상당수의 환자에서 혈액 채취만으로도 G6Pase 유전자 분석을 통한 GSD 진단이 손쉽게 이루어질 수 있으리라고 생각된다.

• 생명과학회지
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• 제30권9호
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• pp.813-818
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• 2020

고세균 유전체들 사이의 공통적 유전자를 파악하는 archaeal clusters of orthologous genes (arCOG) 알고리즘으로, 168 균주의 고세균들에 공통적인 보존적 유전자를 파악하고자 하였다. 보존된 ortholog의 수는 168, 167, 166 및 165 균주에서 각각 14, 10, 9 및 8개였다. 이들 41개의 arCOG 중에서 번역, 리보솜 구성 및 생합성에 관련된 arCOG가 13개로 가장 많았고 DNA의 복제와 재조합 및 수복에 관련된 arCOG가 10개로 다음으로 많았다. 168개의 고세균 균주들에 보존적인 14개의 arCOG들은 tRNA synthetase가 6개로 가장 많았다. 리보솜과 반응, tRNA 합성, DNA 복제, 전사와 관련한 arCOG가 각 2개씩으로 고세균에서 단백질 발현의 중요성을 알 수 있었다. 보존적 arCOG 구성원들의 distance value의 평균으로 3개 이상의 구성원을 가지는 강(class)과 목(order) 수준에서 유전체를 분석한 결과, Euryarchaeota 문의 Archaeoglobi 강과 Thermoplasmata 강이 각각 최저치와 최고치를 나타내었다. 본 연구는 기초과학 연구와 함께 항균제 개발 및 종양 제어 등에 필요한 자료를 제공할 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제25권5호
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• pp.648-657
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• 2015

H9, a novel herbal extract, demonstrated cytotoxicity in A549 non-small cell lung cancer (NSCLC) cell lines. In this study, we investigated whether H9, and/or co-treatment with an anticancer drug, pemetrexed (PEM), inhibited tumor growth in BALB/c nude mice models bearing A549 NSCLC cells. The mice were separated into groups and administered H9 and PEM for 2 weeks. Protein and mRNA levels were detected using western blotting and reverse transcription polymerase chain reaction, respectively; immunohistochemistry (IHC) was also performed on the tumor tissues. H9 and co-treatment with PEM induced the cleavage of proapoptotic factors, such as caspase-3, caspase-8, caspase-9, and poly(ADP)-ribose polymerase (PARP). Expression levels of cell-death receptors involving Fas/FasL, TNF-related apoptosisinducing ligands (TRAIL), and TRAIL receptors were increased by H9 and co-treatment with PEM. Furthermore, analysis of levels of cell-cycle modulating proteins indicated that tumor cells were arrested in the G1/S phase. In addition, the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt survival signaling pathways were inhibited by H9 and co-treatment with PEM. In conclusion, H9 and co-treatment with PEM inhibited tumor growth in BALB/c nude mice models bearing A549 NSCLC cells. These results indicate that H9 and co-treatment with PEM can be used as an anticancer therapy in NSCLC.

• The Korean Journal of Physiology and Pharmacology
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• 제9권4호
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• pp.231-238
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• 2005

Neuronal apoptotic events, which result in cell death, are occurred in hypoxic/ischemic conditions. Estradiol is a female sex hormone with steroid structure known to provide neuroprotection through multiple mechanisms in the central nervous system. This study was aimed to investigate the signal transduction pathway of $CoCl_2$-induced neuronal cell death and the inhibitory effects of estradiol. Administration of $CoCl_2$ decreased cell viability in both a dose- and time-dependent manner in PC12 cells. $CoCl_2$-induced cell death produced genomic DNA fragmentation and morphologic changes such as cell shrinkage and condensed nuclei. It was found that $CoCl_2$-treated cells increased the reactive oxygen species (ROS) as well as caspase-8, -9 and -3 activities. However, pretreatment with estradiol before exposure to $CoCl_2$ prevented the reduction in cell viability reduction and attenuated DNA fragmentation and morphologic changes caused by $CoCl_2$. Furthermore, the $CoCl_2$-induced increases of ROS levels and caspases activities were attenuated by estradiol. Gene expression analysis revealed that estradiol blocked the underexpression of the Bcl-2 and ameliorated the increase in the release of cytochrome c from mitochondria into cytoplasm and Fas-ligand (Fas-L) upregulated by $CoCl_2$. These results suggest that $CoCl_2$ induce apoptosis in PC12 cells through both mitochondria- and death receptor-mediated cell death pathway. Estradiol was found to have a neuroprotective effect against $CoCl_2$-induced apoptosis through the inhibition of ROS production and by modulating apoptotic effectors associated with the mitochondria- and death-dependent pathway in PC12 cells.

• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.303-310
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• 2015

The study was conducted to analyze the genetic parameters of somatic cell score (SCS) of Holstein cows, which is an important indicator to udder health. Test-day records of somatic cell counts (SCC) of 305-day lactation design from first to fifth lactations were collected on Holsteins in Korea during 2000 to 2012. Records of animals within 18 to 42 months, 30 to 54 months, 42 to 66 months, 54 to 78 months, and 66 to 90 months of age at the first, second, third, fourth and fifth parities were analyzed, respectively. Somatic cell scores were calculated, and adjusted for lactation production stages by Wilmink's function. Lactation averages of SCS ($LSCS_1$ through $LSCS_5$) were derived by further adjustments of each test-day SCS for five age groups in particular lactations. Two datasets were prepared through restrictions on number of sires/herd and dams/herd, progenies/sire, and number of parities/cow to reduce data size and attain better relationships among animals. All LSCS traits were treated as individual trait and, analyzed through multiple-trait sire models and single trait animal models via VCE 6.0 software package. Herd-year was fitted as a random effect. Age at calving was regressed as a fixed covariate. The mean LSCS of five lactations were between 3.507 and 4.322 that corresponded to a SCC range between 71,000 and 125,000 cells/mL; with coefficient of variation from 28.2% to 29.9%. Heritability estimates from sire models were within the range of 0.10 to 0.16 for all LSCS. Heritability was the highest at lactation 2 from both datasets (0.14/0.16) and lowest at lactation 5 (0.11/0.10) using sire model. Heritabilities from single trait animal model analyses were slightly higher than sire models. Genetic correlations between LSCS traits were strong (0.62 to 0.99). Very strong associations (0.96 to 0.99) were present between successive records of later lactations. Phenotypic correlations were relatively weaker (<0.55). All correlations became weaker at distant lactations. The estimated breeding values (EBVs) of LSCS traits were somewhat similar over the years for a particular lactation, but increased with lactation number increment. The lowest EBV in first lactation indicated that selection for SCS (mastitis resistance) might be better with later lactation records. It is expected that results obtained from these multi-trait lactation model analyses, being the first large scale SCS data analysis in Korea, would create a good starting step for application of advanced statistical tools for future genomic studies focusing on selection for mastitis resistance in Holsteins of Korea.