The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Diversity of Agaricus bisporus Strains by PCR Polymorphism

PCR 다형성에 의한 양송이(Agaricus bisporus) 계통의 유전적 다양성 분석

  • Min, Kyong-Jin (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Kim, Jong-Kun (JK BioTech. Co. Ltd.) ;
  • Kwak, A-Min (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, Youn-Hee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
  • 민경진 (한경대학교 미래융합기술대학원) ;
  • 김종군 ((주)제이케이바이오테크) ;
  • 곽아민 (한경대학교 미래융합기술대학원) ;
  • 공원식 (농촌진흥청 국립특작과학원 버섯과) ;
  • 오연희 (농촌진흥청 국립특작과학원 버섯과) ;
  • 강희완 (한경대학교 미래융합기술대학원)
  • Received : 2014.03.04
  • Accepted : 2014.03.15
  • Published : 2014.03.31
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Abstract

Twelve Universal fungal PCR fingerprint (UFPF) primers that were modified from Universal rice primer (URP) were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 strains of other Agaricus spp. Eight primers, UFPF1, UFPF2, UFPF3, UFPF7, UFPF9, UFPF10, UFPF11, and UFPF12 produced PCR polymorphic bands within and between the Agaricus species. Primer UFPF7 produced specific PCR polymorphic bands that are distinct Korean strain from different strains. Ninety five PCR polymorphic bands were inputted for UPGMA cluster analysis. Forty five strains of A. bisporus are genetically clustered into 8 groups, showing coefficient similarity from 0.75 to 0.9 among them. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with close genetic relationship of coefficient similarity over 0.96, whereas, other Korean strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese.

본 연구에서는 국내외에서 수집한 A. bisporus 45계통과 Agaricus spp. 19균주를 포함한 64균주로부터 genomic DNA를 추출하고 12종류의 UFPF primer (JK Biotech., Ltd.)를 사용하여 PCR 다형성 분석을 실시한 바 양송이 계통간 PCR다양성이 관찰되었다. 7종류의 UFPF primer에 의해 생성된 A. bisporus계통의 95 PCR다형성 밴드가 유전적 유사도 산출에 이용되어 UPGMA cluster분석을 적용 dendrogram을 작성한 결과 A. bisporus의 계통군은 3 cluster 내에 8개의 세부 group으로 분류되었으며 유사성 함수가 0.75에서 0.90의 유연관계를 보였다. 한국에서 최근 개발된 새아, 새정, 새연, 새도는 같은 group에 포함되었으며 0.96%에서 1.0%의 밀접한 근연관계에 있었으며 그밖에 한국품종은 미국 유럽의 양송이 품종과 유전적 유연관계를 보였다.

Keywords

References

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