• 농업과학연구
• /
• 제43권1호
• /
• pp.109-115
• /
• 2016

Silk has had a reputation as a luxurious and sensuous fabric but it is not popular due to the expensive price and poor durability. To develop the silk materials that apply the various industries, the artificially synthesized gene can be introduced into the silkworm and expressed in the silk gland. Transgenic silkworms for the mass production of green fluorescent silks are generated using a fibroin H-chain expression system. For commercial use, safety assessment of the transgenic silkworms is essential. The purpose of this study was to examine the potential acute oral toxicity of EGFP protein expressed in genetically modified (GM) fluorescence silkworm and to obtain the approximative lethal dose in the male and female at 6-weeks ICR mice. EGFP protein was fed at a dose of 2,000 mg/kg body weight in five male or five female mice. Mortalities, clinical findings and body weight changes were monitored for 1, 3, 7, 14 days after dosing. At the end of 14 day observation period, all mice were sacrificed, and the postmortem necropsy were performed. The test group was not observed death case. Also the effect was not admitted by test substance administration in common symptoms, the body weight and postmortem. The results of single-dose oral toxicity test showed that approximative lethal dose of EGFP protein expressed in fluorescence silkworm was considered to exceed the 2,000 mg/kg body weight in both sexes.

• Journal of Microbiology and Biotechnology
• /
• 제26권7호
• /
• pp.1303-1310
• /
• 2016

Although many studies on the effects of genetically modified (GM) crops on soil microorganisms have been carried out over the past decades, they have provided contradictory information, even for the same GM crop, owing to the diversity of the soil environments in which they were conducted. This inconsistency in results suggests that the effects of GM crops on soil microorganisms should be considered from many aspects. In this study, we investigated the effects of the GM drought-tolerant rice MSRB2-Bar-8, which expresses the CaMSRB2 gene, on soil microorganisms based on the culture-dependent and culture-independent methods. To this end, rhizosphere soils of GM and non-GM (IM) rice were analyzed for soil chemistry, population densities of soil microorganisms, and microbial community structure (using pyrosequencing technology) at three growth stages (seedling, tillering, and maturity). There was no significant difference in the soil chemistry between GM and non-GM rice. The microbial densities of the GM soils were found to be within the range of those of the non-GM rice. In the pyrosequencing analyses, Proteobacteria and Chloroflexi were dominant at the seedling stage, while Chloroflexi showed dominance over Proteobacteria at the maturity stage in both the GM and non-GM soils. An UPGMA dendrogram showed that the soil microbial communities were clustered by growth stage. Taken together, the results from this study suggest that the effects of MSRB2-Bar-8 cultivation on soil microorganisms are not significant.

• Journal of Biosystems Engineering
• /
• 제40권3호
• /
• pp.224-231
• /
• 2015

Purpose: Corn is a major commercial crop targeted for genetic modification owing to its high consumer demand as a foodstuff for humans and livestock, as well as its other industrial applications. However, the safety of genetically modified (GM) crops is controversial. Indeed, several countries have banned the importation of GM seeds that can germinate. Therefore, development of effective, convenient, and nondestructive methods to inhibit seed germination is required. Methods: This study aimed to examine the efficacy of microwave heat treatment for inhibition of germination of corn kernels and for optimization of power and exposure time required for effective aging treatment. Artificial inhibition was induced in corn kernels using microwave heat treatment. Seven power levels were examined (400, 500, 600, 700, 800, 900, and 1000 W) at each of the four exposure times (0.5, 1.0, 1.5, and 2.0 min). Results: Corn kernels could be aged effectively after heating for 0.5~1.0 min at powers greater than 800 W, with increasing efficacy observed at higher powers. Further analysis showed that the most effective inhibition of germination was observed at 1000 W for 40 s. This setting did not cause any physical damage to the corn kernels. Conclusions: Optimal inhibition of corn kernel germination was achieved using higher power for shorter times, which may be useful for industrial corn seed treatment.

• 농업과학연구
• /
• 제41권3호
• /
• pp.157-161
• /
• 2014

Understanding the gene flow from genetically modified (GM) crops to conventional crops is important to prevent and mitigate seed contamination caused by pollen-mediated gene flow. We conducted a field test to investigate the gene flow from diamondback moth resistant GM cabbage (Brassica oleracea var. capitata) containing cry1Ac1 gene, to a non-GM control line AD126. GM and non-GM cabbage plants were cultivated in the field and pollinated using Bombus terrestris under the nets during the flowering periods. After seeds were collected from non-GM plants, hybrids between them and the GM cabbages were screened by multiplex PCR targeting cry1Ac1 gene. Out of 878 germinated seedlings, 168 hybrids were found and the average gene flow frequency was 19.7%. Because cabbage is mainly pollinated by insect pollinators, large-scale field tests are needed to study gene flow of GM cabbage.

• 한국환경농학회지
• /
• 제31권2호
• /
• pp.192-199
• /
• 2012

유전자변형 작물을 종자로 판매하거나 식품, 사료 혹은 가공용으로 이용하기 위해서는 반드시 관련 기관의 승인을 받아야 한다. 관련부처에서는 유전자변형 작물의 승인에 앞서 환경위해성 평가 자료가 과학적으로 타당한지 검토한다. 환경위해성 평가 중 유전자변형 작물이 토양 미생물 군집에 미치는 영향은 충분히 연구되지 못한 분야이다. 최근 토양 환경내 미생물 군집의 특성을 연구하기 위한 발전된 방법들이 개발되고 있다. 배양에 의존적인 또는 비의존적인 기술에 의한 토양 미생물의 군집 특성을 조사한 연구와 유전자변형 작물의 환경위해성 평가 적용 가능성을 고찰하였다. 유전자변형 작물의 토양미생물 영향 평가는 사안별 평가 원칙에 의해 이루어져야 한다. 신뢰할 수 있고 상세한 토양 미생물 평가가 이루어지기 위해서는 다양한 분석 방법의 조합이 필요하다.

• 한국응용곤충학회지
• /
• 제56권4호
• /
• pp.331-338
• /
• 2017

본 연구에서 국내에서 개발된 제초제저항성 GM 벼인 밀양 204호와 익산 483호가 비표적 곤충과 거미에 미치는 영향을 평가하였다. GM 벼와 non-GM 벼에 대한 보리수염진딧물과 벼멸구의 기주선호도에는 차이가 없었으며, GM 벼를 섭식한 벼멸구를 포식한 황산적거미의 체중 변화에도 통계적 유의성이 없었다. 밀양 204호와 그 대조 모본 non-GM 벼에서 서식한 벼멸구를 포식한 황산적거미의 생존율에는 차이가 없었으나, 벼멸구를 통해 익산 483호에 노출된 황산적거미의 생존율은 대조 모본 non-GM 벼에 비해 통계적으로 낮은 생존율을 나타내었다. 또한 익산 483호의 화분을 섭식한 꿀벌 유충은 밀양 204호와 다른 non-GM 벼 화분을 제공하였을 때 보다 현저하게 긴 용기간을 보였다. 농업생태계에서 중요한 포식 천적인 황산적거미와 양봉 산업으로서 중요한 기능을 하는 꿀벌에 GM 벼가 부정적인 영향을 보임에 따라 국내에서 개발된 제초제저항성 GM 벼의 농업 환경 노출 이전에 충분한 추가 연구와 안전성 평가가 선행되어야 할 것으로 사료된다.

• Journal of Plant Biotechnology
• /
• 제45권4호
• /
• pp.289-298
• /
• 2018

국립농업과학원 생물안전성과는 생명공학기술로 개발되는 작물(GM작물)의 안전성 평가 중 '성분분석에 의한 비교평가'에 참고자료로 활용할 수 있도록 기존 상업화 작물성분의 분석 데이터를 제공하는 "작물성분 DB"를 구축하였다. "작물성분 DB"는 우리나라의 자연 환경에서 재배되는 주요 작물 품종들의 영양성분 함량정보를 제공함으로써 품종과 재배 지역, 연도에 따라 함량의 변화 정도를 확인할 수 있도록 데이터를 업그레이드 하고 있다. 현재 2곳 이상의 재배지역에서 2년 이상 재배한 벼와 고추 시료에 대한 주요 영양분과 항영양소, 2차 대사산물을 검증된 분석 방법을 사용하여 분석한 데이트를 제공하고 있다. 데이터는 평균값과 최저, 최대값을 제공함으로써 GM작물의 안전성 평가시 GM작물과 대응 비교작물 간에 나타나는 통계적 차이가 기존 상업작물의 생물학적 차이 또는 허용범위 내에 속하는지를 평가할 수 있도록 하였다. "작물성분 DB"는 사용자가 선택한 쿼리를 기반으로 쉽게 검색하고 활용하도록 하고 있다. 또한 유색미와 감자, 고구마, 콜리플라워와 같은 유색 작물의 기능성 성분 함량 정보를 제공하고 있어 일반 소비자들도 유용하게 정보를 활용할 수 있다. 본 논문은 상업작물 성분에 대한 중요한 정보를 제공하는 농진청 "작물성분 DB"의 구성과 사용법을 소개하였다.

• 한국작물학회지
• /
• 제45권1호
• /
• pp.59-70
• /
• 2000

In nature, plant diseases, insects and parasites (hereafter called as "pest") must be co-survived. The most common expression of co-survival of a host crop to the pest can be tolerance. With tolerance, chemical uses can be minimized and it protects environment and sustains host productivity and the minimum pest survival. Tolerance can be applicable in all living organisms including crop plants, lifestocks and even human beings. Tolerant system controls pest about 90 to 95% (this pest control system often be called as horizontal or partial resistance), while the use of chemicals or selection of high resistance controls pest 100% (the most expression of this control system is vertical resistance or true resistance). Controlling or eliminating the pests by either chemicals or vertical resistance create new problems in nature and destroy the co-survial balance of pest and host. Controlling pests through tolerance can only permit co-survive of pests and hosts. Tolerance is durable and environmentally-friend. Crop cultivars based on tolerance system are different from those developed by genetically modified organism (GMO) system. The former stabilizes genetic balance of a pest and a host crop in nature while the latter destabilizes the genetic balance due to 100% control. For three decades, the author has implemented the tolerance system in breeding maize cultivars against various pests in both tropical and temperate environments. Parasitic weed Striga species known as the greatest biological problem in agriculture has even been controlled through this system. The final effect of the tolerance can be an integrated genetic pest management (IGPM) without any chemical uses and it makes co-survival of pests in nature.in nature.

• 한국작물학회지
• /
• 제47권1호
• /
• pp.62-67
• /
• 2002

An efficient system of rice microspore culture could contribute to the production of genetically modified rice. The microspores were isolated by mechanical or shed methods. The number of microspores per 100 anthers isolated at uninucleate stage was higher than (or similar to) those at binucleate stage in isolation method with pestle or spatular, but microspore divisions were not easily observed on both stages. On the other hand, pollen division in shed pollen culture was observed more frequently at uninuclear than at binuclear stage. Cold pretreatment at 1$0^{\circ}C$ for 10 days resulted in the best multicellular division to produce microcalli at 12.5% efficiency in shed microspores. Heat shock at 33$^{\circ}C$ for one hour before or after pollen shedding enhanced cell division and callus formation. Out of twelve green regenerants, two were haploids and ten were diploids based on the chromosome analysis of root tips. The size of stoma was 12$^{m}$ m in haploids and 15 ${\mu}{\textrm}{m}$ in diploids determined by scanning electron microscope (SEM).

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.68-68
• /
• 2017

The status of corn genetic diversity in the uplands of Sarangani in Southern Philippines was investigated using 12 morphological traits subjected to multivariate statistical analyses. Information about traditional farming, post-harvest and storage practices were also elicited especially in relation to losses of traditional varieties, a phenomenon known as genetic erosion. While a handful of farmers still plant traditional corn varieties in the remotest areas, a significant number had already shifted to genetically modified corn. Furthermore, principal component analysis (PCA) reduced the 12 morphological traits into 5 principal components and identified ear length and ear weight to be major contributors to variation. Cluster Analysis, on the other hand, formed two distinct groups but failed to give information about intra-cluster variability among the 32 collected corn accessions. These results warrant that more informative morphological traits and that molecular markers will be used to obtain a better picture of genetic diversity in Sarangani upland corn. Molecular analysis is also needed to establish genetic identities of these cultivars and to detect gene introgression from GM varieties into the gene pool of farmers' corn varieties. These analyses are imperative for the conservation of traditional corn varieties before they disappear in the Sarangani uplands because of shifting priorities of upland farmers.