• Food Science and Biotechnology
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• 제15권4호
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• pp.485-493
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• 2006

Lactococcus species are noninvasive and nonpathogenic microorganisms that are widely used in industrial food fermentation and as well-known probiotics. They have been modified by traditional methods and genetic engineering to produce useful food-grade materials. The application of genetically modified lactococci in the food industry requires their genetic elements to be safe and stable from integration with endogenous food microorganisms. In addition, selection for antibiotic-resistance genes should be avoided. Several expression and secretion signals have been developed for the production and secretion of useful proteins in lactococci. Food-grade systems composed of genetic elements from lactic acid bacteria have been developed. Recent developments in this area have focused on food-grade selection markers, stabilization, and integration strategies, as well as approaches for controlled gene expression and secretion of foreign proteins. This paper reviews the expression and secretion signals available in lactococci and the development of food-grade markers, food-grade cloning vectors, and integrative food-grade systems.

• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.274-279
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• 2012

For the quantitative analysis of genetically modified (GM) maize in processed foods, primer sets and probes based on the 35S promoter (p35S), nopaline synthase terminator (tNOS), p35S-hsp70 intron, and zSSIIb gene encoding starch synthase II for intrinsic control were designed. Polymerase chain reaction (PCR) products (80~101 bp) were specifically amplified and the primer sets targeting the smaller regions (80 or 81 bp) were more sensitive than those targeting the larger regions (94 or 101 bp). Particularly, the primer set 35F1-R1 for p35S targeting 81 bp of sequence was even more sensitive than that targeting 101 bp of sequence by a 3-log scale. The target DNA fragments were also specifically amplified from all GM labeled food samples except for one item we tested when 35F1-R1 primer set was applied. A reference plasmid pGMmaize (3 kb) including the smaller PCR products for p35S, tNOS, p35S-hsp70 intron, and the zSSIIb gene was constructed for real-time PCR (RT-PCR). The linearity of standard curves was confirmed by using diluents ranging from $2{\times}10^1{\sim}10^5$ copies of pGMmaize and the $R^2$ values ranged from 0.999~1.000. In the RT-PCR, the detection limit using the novel primer/probe sets was 5 pg of genomic DNA from MON810 line indicating that the primer sets targeting the smaller regions (80 or 81 bp) could be used for highly sensitive detection of foreign DNA fragments from GM maize in processed foods.

• 한국지역사회생활과학회지
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• 제15권4호
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• pp.177-184
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• 2004

본 연구는 농촌진흥청에서 개발한 유전자 변형(제초제 저항성) 고추와 모종인 수비초 고추의 영양성분을 분석하고 유전자 변형된 경우 영양성분에 차이가 있는지 비교하기 위하여 수행하였다. 영양성분은 AOAC법을 이용하여 주요 영양소(단백질, 지방, 섬유소, 회분, 당질)와 무기질(칼슘, 칼륨, 나트륨, 마그네슘, 아연, 철) 함량을 분석하였고, 식품성분표에 제시된 일반 고추의 영양성분과 비교하였다. 그 결과 유전자 변형 고추는 모종 고추와 영양성분 함량이 유사하였고, 일반적인 고추의 영양성분 함량 범위 내에 있어 실질적인 동등성을 갖는 것으로 나타났다.

• 가정과삶의질연구
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• 제21권2호
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• pp.19-30
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• 2003

In this study, housewives' attitudes toward genetically modified foods (GM foods) and their willingness to purchase GM foods were examined. The findings of this study could provide useful information for consumer education and consumer policy development regarding GM foods. The specific purposes of this study were: (1) to examine consumers' attitudes toward GM foods, (2) to analyze the effect of the perception of GM foods and demographic variables on consumers' attitudes toward GM foods, and (3) to analyze the effect of the perception of GM foods and demographic variables on consumers' willingness to purchase GM foods. The questionnaire used in the survey was constructed by the author, based on existing literature. The survey was conducted with 1,100 housewives, and 723 of the completed survey forms were used in the final analysis. Frequencies, percentages, means, standard deviation, t-tests, ANOVA, Duncan-test, Pearson's Correlation, factor analysis, and discriminant analysis were employed for data analysis methods. Major findings are: (1) Consumers' attitudes toward GM foods consist of three factors, that are, attitude regarding potential danger, attitude regarding the use of GM technique on plants, and attitude regarding the use of GM technique on animals. (2) Consumers with a higher level of education tend to perceive GM food as more dangerous, whereas consumers with a lower level of education tend to accept more the use of GM technique on plants. (3) Consumers who tend not to consider GM foods as dangerous, and those who acknowledged benefits in using GM technique on plants are more willing to buy GM foods.

• 한국식품위생안전성학회지
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• 제17권3호
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• pp.157-165
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• 2002

유전자재조합식품의 안전성과 표시에 관한 일반국민의 의식을 2000년과 2001년 5웜과 9월 사이에 각각 750명과 979명을 대상으로 설문조사하였다. 평균 67.7%의 응답자가 유전자재조합식품에 관한 정보를 접한 경험이 있었다. 유전자재조합식품이 줄 수 있는 최대의 이점은 식량난해결이라는 의견을 나타냈다. 유전자재조합식품에는 90%이상의 응답자가 표시하기를 바랐으며 현재 구입하여 먹겠다는 사람은 18%이었고 기다려보겠다는 관망형이 49%를 넘어서 표시를 희망하면서도 결정을 유보하는 사람의 비율이 많았다. 유전자재조합기술로 개발된 제초제 내성 콩을 먹겠다는 사람보다 같은 방법으로 개발된 비타민 함량이 높은 콩을 먹겠다는 사람이 상대적으로 많은 것으로 보아 제품의 특성을 중시하지 유전자재조합 여부에는 큰 관심을 두지 않는 것으로 나타났다. 우리가 먹는 생물 유래의 식품에 유전자가 있다는 사실을 바르게 알고있는 응답자는 전체의 40% 정도였다. 유전자재조합식품이 위험할 것이라고 생각하는 사람은 지극히 적어서 응답자의 4%정도로 유지하였다. 유전자재조합 옥수수인 스타링크의 뉴스를 접한 경험이 있는 응답자가 66%나 되었던 반면 2001년 7월부터 실시된 유전자재조합 표시제의 시행에 대해 알고있는 소비자는 22%에 지나지 않았다.

• 한국축산식품학회지
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• 제31권5호
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• pp.658-662
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• 2011

In this study, 543 samples of press hams, sausages, processed ground meat and processed cheese acquired from retail markets in Seoul and Gyeonggi province in Korea from 2005 to 2010 were monitored using a one-step multiplex polymerase chain reaction (PCR) method that involves the amplification of specific soya or maize endogenous genes and the amplification of 35S promoter (p35S) and nopaline synthase terminator (tNOS) for GMO detection. Among the 543 samples, 477 samples were amplified for maize and/or soybean endogenous genes. Although one sausage sample collected in 2008 showed amplification of tNOS, the result was assumed to be false positive based on the results from further tests of other sausage samples of the same brand. Our results demonstrate the absence of GM soya and/or maze of livestock products in the Korean market during 2005-2010. In addition, the one-step multiplex PCR using previously constructed primer sets appears to be useful as a screening method for the detection of GMOs in processed livestock products. However, more specific methods should be established and employed to detect the event-specific GM gene for positive reaction samples by screening tests in processed livestock products.

• 한국식품영양과학회지
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• 제39권1호
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• pp.105-109
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• 2010

본 연구는 국내에서 개발된 베타-카로틴 강화 유전자변형 벼(GM 벼)와 그 모종 벼인 낙동벼(non-GM 벼)로부터 쌀(현미, 백미)의 일반성분, 베타-카로틴, 지방산, 아미노산, 무기질 함량을 분석하여 유전자변형 쌀에서 주요 영양성분 조성에 차이가 있는지를 비교하기 위해 수행되었다. GM 쌀은 non-GM 모종 쌀과 일반성분 함량이 유사하였고 GM 쌀 100 g당 약 0.2 mg의 베타-카로틴을 함유하는 것으로 분석되었다. GM 쌀의 지방산 조성은 oleic acid, linoleic acid, palmitic acid가 지방산 전체의 94% 이상으로 거의 대부분을 차지하고 있었으며 non-GM 쌀과 전체적인 지방산 조성이 유사한 것으로 나타났다. GM 쌀의 아미노산은 Asp>Arg>Leu>Ala>Ser>Val 순으로 그 함량이 높았고 무기질 함량은 P>K>Mg>Na>Ca>Zn>Fe의 순이었으며, non-GM 쌀에 비해 대부분의 아미노산과 무기질 함량이 약간 높은 수치로 측정되었지만 유의적인 차이는 없는 것으로 평가되었다. 베타-카로틴 강화 유전자변형 쌀은 의도한 바의 베타-카로틴이 쌀의 종실에 생합성 됨과 함께 쌀의 일반성분, 지방산, 아미노산 및 무기질 함량에는 별 다른 영향을 미치지 않는 것으로 나타났다.

• 농촌지도와개발
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• 제7권2호
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• pp.333-345
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• 2000

The objectives of this study were to review various aspects of society on Genetically Modified Organism(GMO) in the view point of environmental sociology. There were many assertions related to GMO, and application of biotechnology. Some people assert GMO as harmful to human health, and some people as not harmful, however, there were no proven evidences to report. Through the literature review, this study intended; 1) to identify the present condition of GMO, 2) to weigh the pros against cons of views on GMO, 3) to study an aspect of society on GMO, and 4) to assert its necessity to mark GMO or not, because citizens have a rights to know natural or GMO. Based on the conclusions of this study, following recommendations were offered: 1) To review continually the GMO in the view point of environmental sociology especially in the areas of food safety, biosafety, trade, and public research investment, and 2) further detailed studies of GMO may necessary to ensure safety to human health and environment.

• Weed & Turfgrass Science
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• 제3권3호
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• pp.221-224
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• 2014

Genetically modified (GM) crops are not permitted to be cultivated in Korea, but can only be imported as food or feed purposes. The import of GM crops has sharply increased in recent years, thus raising concerns with regard to the unintentional escape of these crops during transport and manufacturing as well as the subsequent contamination of local, non-GM plants. Hence, monitoring of GM crops was studied in or outside of grain receiving ports as well as from feed-processing plants in Korea during July 2008. We observed spilled maize grains and established plants primarily in storage facilities that are exposed around the harbors and near transportation routes of the feed-processing areas. Based on the PCR analyses, a total of 17 GM maize plants and 11 seeds were found among the samples. In most cases, the established maize plants found in this study were at the vegetative stage and thus failed to reach the reproductive stage. This study concludes that, in order to prevent a genetic admixture in the local environment for GM crops or seeds, frequent monitoring work and proper action should be taken.

• 한국식품위생안전성학회지
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• 제18권1호
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• pp.6-13
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• 2003

PCR법은 특정 유전자를 증폭하는 기술로 작물 및 식품에서 유전자 변형체 함유 여부를 가리는 효과적인 방법이다. 그러나 핵산의 추출법에 따라 PCR의 감도가 크게 달라지므로 정확한 추출법의 선정이 매우 중요하다. 본 연구는 현재 컬럼형 상용화 키트와 기존의 용매 추출방법을 이용하여 콩과 옥수수가공식품에 대한 각 유전자 부위의 검출감도를 비교 분석하였다. 핵산의 추출효율과 도입유전자의 증폭효율면 모두 상용화 키트인 Wizar$d^{TM}$, DNeasy$^{TM}$ 추출법이 우수하였다. DNeasy법은 대부분의 식품에서 우수한 추출효율을 보였으나, 옥수수가공식품에서 수율이 감소하는 단점을 나타내었다. Wizard법은 모든 가공식품에서 고른 추출효율을 보였으며, PCR반응에 의한 증폭산물도 잘 보존되어 가공식품의 GMO 검출에 적합한 것으로 나타났다. 한편 CTAB법은 콩가공식품에서 약간 효율이 좋은 것으로 나타났으나 대부분의 경우 효율이 낮았으며, 식품의 종류에 따라 편차가 심하게 나타났다. phenol/chloroform법은 대부분의 식품에서 핵산의 분리가 어려운 방법으로 나타나 GMO분석에는 적합하지 않은 방법으로 확인되었다.