• 농업과학연구
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• 제43권4호
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• pp.560-566
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• 2016

Imports of genetically modified (GM) soybeans (Glycine max) for food or feed consumption in Korea have been increasing. Although the cultivation of GM soybeans has not yet been allowed in Korea, the number of field tests for GM soybeans has also been rising. This study was conducted to investigate whether herbicide tolerant GM soybean can survive and persist in uncultivated environments when they escape from transportation routes or from isolated fields. Seeds of GM and non-GM soybeans and wild soybeans (Glycine soja) were buried in 2 and 15 cm soil depths and their viability was examined after 1, 2, 6, and 10 months. GM and non-GM soybean seeds completely lost their viability within six months of burial, whereas seeds of wild soybean maintained their viability during the study period. Seeds of soybean and wild soybeans that were sown on the soil surface germinated and grew to vegetative cotyledon stage. Seedlings of GM and non-GM soybean did not compete well with weeds, including Cerastium glomeratum, Alopecurus aequalis var. amurensis, Capsella bursa-pastoris, Conyza canadensis, Stellaria aquatica, and Erigeron annuus. Also, GM soybean did not survive through winter. However, wild soybeans competed well with the weeds and became dominant in August. Herbicide tolerant GM soybean is unlikely to persist under uncultivated environments and to become weeds.

• 한국환경보건학회지
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• 제31권5호
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• pp.431-443
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• 2005

This study was performed to investigate the perceptions of the opinion leaders, such as government officials, researchers, NGO workers, and journalists on the risks of endocrine disrupters (EDs) and genetically modified organisms (GMOs) as well as the related policies on these two hazards or potential hazards. The opinion leaders generally considered the EDs as the most serious hazard among twenty-one environmental health hazards in Korea, and agreed that the EDs would continuously be the most serious hazard. On overall average, the GMOs were ranked the 11th among the twenty-one health hazards. Further investigation indicated that the GMOs were variously ranked by the group of respondents: they were ranked the 2nd by the NGO workers, the 7th by the journalists, the 9th by the researchers and the 11th by the government officials. In general, the respondents considered the dioxin as a hazard with the highest risk while the GMOs were considered less hazardous. The opinion leaders considered that although the risks of the GMOs and EDs were not fully verified, the risks should be controlled through the legislation. The EDs and GMOs should be separately regulated for the time being, while the EDs should put under more strengthened regulation. It is recommended that a web-site containing the information on the EDs and the GMOs be prepared for the journalists. In addition, a training program in relation to the EDs and the GMOs needs to be organized by the Korean Press Foundation and the Korea Food and Drug Administration to educate the journalists. A committee consisting of government officials, scientists, and NGO workers needs to be established, and it should provide framework of future policies and public relations programs.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.201-206
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• 2021

우리나라는 유전자변형생물체(GMO)의 개발 및 수입 등에 관한 안전성 확보를 위하여 LMO법을 제정하였다. 이에 따라 국내에서 GMO가 식품 및 사료용으로 수입, 생산될 경우는 OECD가 제시한 '실질적 동등성' 개념에 따라 안전성 심사를 한다. 안전성 심사에는 GMO에 대한 알레르기 평가심사가 포함된다. 알레르기성 평가 방법은 OECD, FAO, WHO 등 다양한 국제기구를 통해 논의되어왔다. 주요 알레르기성 평가 방법으로는 GMO에서 새로 발현된 단백질의 물리 화학적 감수성 확인, 기존 알레르기 단백질과의 아미노산 서열 유사성 분석, 혈청 스크리닝 등이 있다. 따라서 본 연구에서는 유전자변형생물체의 알레르기성 평가를 위한 가이드라인과 관련 연구들을 소개하였다.

• 의료법학
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• 제12권1호
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• pp.43-67
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• 2011

Humankind history is faced with one gigantic turning point due to development of Living genetically Modified Organisms. Food production by means of LMO is on the acceleration in an effort to solve the shortage of food problems. Food is also used as alternative energy source. Use of LMO product is not only limited to food and energy, but is actively utilized in various fields of medicines. This paper is first to check out the state of biomedicine developed and associated problems from industries that use LMO, after which we made an attempt on legislative approach to find out means of relief, through examples of such laws legislated for the sufferer from the adverse effect of the biomedicine. As for the liable subject to bear the responsibility for compensatory damage in a way of relieving the victim owing to adverse effect of biomedicine, those who manufactured and sold biomedicine and who are related to the damage to the victim due to the accident and medical doctors and pharmacists who prescribe and administer the medicine in question have been looked into. Accidents involving medicines and medical supplies could take place without reason for imputation on part of the liable subjects or fault of the victim, in which case the victim can't receive damage compensation from any of both parties. When such accidents happened turn out to be no fault accidents, introduction of damage relief measures might have to be reviewed against side effects of medicine and medical supplies as no fault compensation in order for actual relief to be possible. Talking about technicality of legislation, we can suggest a method of strengthening the accountability of manufacturer for stereotypical agenda on biomedicines by newly legislating special regulation with an issue that resists claim on risks associated with the development of medicine and incorporating the same into Manufactured Product Liability Law. After all, when an accident happens associated with biomedicine, the damage will be done to the consumer. And the consumer will be exposed to fatal danger even without the time to cope with potential risks associated with medicine and medical supplies they take. Therefore, it is necessary to protect the potential victim by having the manufacturer of biomedicines bear the liability of medical risks.

• 한국식품위생안전성학회지
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• 제33권3호
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• pp.193-199
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• 2018

본 연구에서는 유전자 변형 옥수수(GM 옥수수)에 특이한 단크론성 항체를 개발하고 이에 대한 특성을 확인하는 연구를 수행하고자 하였다. 먼저 형질전환 대장균으로부터 PAT 단백질을 발현시킬 수 있는 시스템을 확립하였고, 재조합 PAT 단백질을 대량 생산하여 항원으로 사용하였다. 준비된 항원을 면역한 결과 재조합 PAT 단백질의 항원성은 매우 높은 것으로 확인되었으며, 세포융합과 클로닝을 통해 12 종의 hybridoma를 확립하였고 western blot 결과 10 종의 hybridoma가 재조합 PAT 단백질과 강한 반응성을 나타내었다. 10종의 hybridoma가 생산하는 항체가 실제 GM 옥수수에 반응하는지를 추가의 western blot으로 분석한 결과 2종의 단크론성 항체(PATmAb-7 and PATmAb-12)가 재조합 PAT 단백질뿐만 아니라 실제 GM 옥수수 중 PAT와 반응하는 것으로 확인되었다. 항체를 대량 생산하고 정제한 후 2종의 항체는 SDS-PAGE 상에서 대표적인 항체의 분리패턴(heavy와 light chain)을 나타내었고, 전형적인 $IgG_1$과 ${\kappa}$ type으로 확인되었다. 정제된 단크론성 항체는 특성을 조사한 결과 다른 GMO에서 발현될 수 있는 재조합 단백질과 non-GM 옥수수 추출물에는 반응성이 없고 PAT 단백질에만 특이적으로 반응하는 것을 확인할 수 있었다. PATmAb-7 를 이용한 간접효소면역분석법의 검출한계는 0.3 ng/mL 수준으로 기준의 유전자변형 콩 면역분석법과 비교했을 때 높은 민감도를 나타내었다. 이상의 결과로 볼 때 개발된 2종의 항체(PATmAb-7 and PATmAb-12)는 GM 옥수수에서 발현되는 PAT 단백질에 특이적으로 반응하는 항체로 확인되었고, 2종의 항체를 이용한 면역분석법과 바이오센서의 개발 가능성을 제시할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.25-31
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• 2006

Changes of CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) in the glyphosate-tolerant Roundup Ready soybean were examined using purified CP4 EPSPS produced in cloned Escherichia coli as a control. CP4 EPSPS in genetically modified soybean was detected by twodimensional gel electrophoresis (2-DE) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS) with databases. CP4 EPSPS in soybean products was resolved on 2-DE by first isoelectric focusing (IEF) based on its characteristic pI of 5.1, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) based on its molecular mass of 47.5 kDa. We quantified various percentages of soybean CP4 EPSPS. The quantitative analysis was performed using a 2D software program on artificial gels with spots varying in Gaussian volumes. These results suggested that 2-DE image analysis could be used for quantitative detection of GM soybean, unlike Western blotting.

• 대한영양사협회학술지
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• 제12권4호
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• pp.329-341
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• 2006

The purposes of the study were to examine food purchasing management practices and to assess dietitians' awareness on food quality standards, use of food purchasing guidelines(food quality guidelines, supplier selection criteria), and organizational collaboration related to purchasing at school food service. A total of 400 dietitians working at elementary schools in Kyunggi Province were surveyed and 247 responses were returned. Excluding responses with significant missing data, 240 responses(60%) were used for data analysis. Compared to the previous reports, competitive bidding and shorter contract periods were preferred for purchasing food supplies. A dietitian, school staff, and food service staff participated in receiving and inspection together at 58.4% of the schools. An average score of the dietitians' awareness on the food quality standards was 3.28 based on a 5-point scale(1:strongly disagree, 5:strongly agree). Their awareness scores for the quality standards related to the genetically modified organism foods, organic foods, and pesticide residues were the lowest. The awareness scores increased significantly with their age(p<.001). In general, the food purchasing guidelines were used well at the schools ; the usage levels of the food quality guidelines and supplier selection criteria were significantly different by length of operation(p<.05) and dietitians' age(p<.05), respectively. The dietitians' perception score of the organizational collaboration related to purchasing was rated 3.46 and differed significantly by dietitians' educational background(p<.05). For improving food procurement management, standardized food quality and purchasing criteria need to be developed. As the demands on high quality food supplies increase for school food service, the dietitians should improve their knowledges on the food quality standards and implement effective and creative purchasing methods.

• 한국유기농업학회지
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• 제8권3호
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• pp.17-34
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• 2000

This paper is aim to report the core aspects of Codex guideline for organically grown food which is finalized by FAO/WHO Codex Alimentarius Commission on May 2000 in 28th session of the Codex committee on food labelling. The chapter of animal production had discussed for a long time before it was finalized in Ottawa/canada as well as use of GMO(Genetically modified organism), manure from factory farming, animal welfare, and fodder inputs consisting of at least 85% for ruminants and 80% for non-ruminants. As the guideline for Codex set out the several things which is very difficult for Asian country, Organic farmer in Korea should pay an attention to maintain/increase the fertility and biological activity by cultivation of legumes, green manures or deep-rooting plants in an appropriate multi-annual rotation programme, and incorporation in the soil of organic material from holding producing in accordance with the guidelines. Pest, diseases and weeds should be controlled by choice of appropriate varieties, appropriate rotation, mechanical cultivation, diversified ecosystems, flame weeding, animal weeding and steam sterilization. The use of plant growth hormone, GMO and manure from industrial management system are not allowed, and closed recycling system, rotation, resistant seeds again pest and disease should be practiced in organic farming. But these are not unfortunately practiced in the country. In the conclusion it was strongly suggested to enact the Basic Standard for Korean organic agriculture which contains the core principles of Codex guidelines, and to try the importation of the most advanced theory, skills and technology from leading country in organic farming.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.136-136
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• 2017

In agriculture, the rice is the one of important things. Many farmers and scientists have long tried to increase the yield of rice. So many technologies have been developed these days. One type of technology has given rise to a host of concerns and questions, namely Genetically Modified Organisms (GMOs). The increasing cultivation of GM crops has raised a wide range of concerns with respect to food safety, environmental effects and socio-economic issues and now commercially planted on about 100 million hectares in some 22 developed and developing countries. The scientific evidence concerning the environmental and health impacts of GMOs is still emerging, but so far there is no conclusive information on the definitive negative impacts of GMOs on health or the environment. Nevertheless, public perceptions about GMOs in food and agriculture are divided with a tendency toward avoiding GM food and products in many developed and developing countries. Also Korea is one of that country and is not allow the GMOs now. So I studied whether these GMOs are actually dangerous for environment and whether there are differences in cultivar characteristics such as germination test with TTC tetrazolium, germination test in frozen soil and gene-flow test with glufosinate and strip-bar test. With these experiments, we evaluated the agricultural safety of GM rice and to identify and assess environmental risks.

• Journal of Plant Biotechnology
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• 제38권2호
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• pp.143-150
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• 2011

1994년 처음으로 GM 토마토인 Flavr Savr가 시장에 나온 이후, 2010년 현재 140여 품목의 GM식물이 전 세계적으로 상업화되었다. GM식물들에 대한 안전성 승인여부의 확인 및 표시제관리를 위하여 이들 GM식물내로 도입된 삽입유전자의 정보를 이용한 검정방법이 도입되었으며, 또한 도입유전자의 발현된 단백질을 분석하기 위하여 정성 및 정량을 위한 면역학적 방법이 도입되었다. 본 총설에서는 국내 외적으로 개발된 콩, 옥수수, 카놀라, 면화 등의 GM식물에 적용된 multiplex PCR, real-time PCR 방법과 최신 개발 중인 microarray, 나노기술 등을 활용한 방법들을 조사하였다.