• 한국환경보건학회지
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• 제44권1호
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• pp.31-43
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• 2018

Objectives: Effective genetic toxicology and molecular biology research techniques and strategies that are highly correlated with the carcinogenic inhalation toxicity test and related research are required. The aim of this study was to maximize the utilization of chemical substances to prevent workers' occupational diseases. Methods: We surveyed the literature, domestic and international references, and the status of relevant domestic and foreign professional organizations. Expert advisory opinions were reflected, and experts were consulted by participating in domestic and overseas academic conferences. Results: The current status of domestic and international genotoxic toxicity evaluation was examined through various documents from related organizations. Cell models for in vitro lung toxicology were investigated and summarized, and the human resources and performance results of genetic toxicity studies and pilot projects were compared and analyzed by holding an advisory meeting. We examined domestic and international genotoxicity guidelines and investigated new test methods for the development of genotoxicity and carcinogenicity. Ultimately, we described long-term future predictions, including the implementation of our researchers' recommendations and occupational genetic toxicology forecasts for future worker health protection. Conclusions: This research project aims to establish current genetic toxicology and molecular biology research techniques and strategies that can maximize the linkage with the carcinogenic inhalation toxicity test and research in the future. We expanded the study of genetic toxicity and establish a foundation forgenetic toxicity in accordance with research trends in Korea and abroad.

• Asian-Australasian Journal of Animal Sciences
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• 제24권8호
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• pp.1031-1040
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• 2011

The objective of this study was to characterize factors influencing genetic improvement of dairy cattle for milk production at farm level. Data were accumulated from 305-day milk yields and pedigree information from 1,921 first-lactation dairy cows that calved from 1990 to 2007 on 161 farms in Central Thailand. Variance components were estimated using average information restricted maximum likelihood procedures. Animal breeding values were predicted by an animal model that contained herd-year-season, calving age, and regression additive genetic group as fixed effects, and cow and residual as random effects. Estimated breeding values from cows that calved in a particular month were used to estimate genetic trends for each individual farm. Within-farm genetic trends (b, regression coefficient of farm milk production per month) were used to classify farms into 3 groups: i) farms with negative genetic trend (b<-0.5 kg/mo), ii) farms with no genetic trend (-0.5 kg/$mo{\leq}b{\leq}0.5$ kg/mo), and iii) farms with positive genetic trend (b>0.5 kg/mo). Questionnaires were used to gather information from individual farmers on educational background, herd characteristics, farm management, decision making practices, and opinion on dairy farming. Farmer's responses to the questionnaire were used to test the association between these factors and farm groups using Fisher's exact test. Estimated genetic trend for the complete population was $0.29{\pm}1.02$ kg/year for cows. At farm level, most farms (40%) had positive genetic trend ($0.63{\pm}4.67$ to $230.79{\pm}166.63$ kg/mo) followed by farms with negative genetic trend (35%; $-173.68{\pm}39.63$ to $-0.62{\pm}2.57$ kg/mo) and those with no genetic trend (25%; $-0.52{\pm}3.52$ to $0.55{\pm}2.68$ kg/mo). Except for educational background (p<0.05), all other factors were not significantly associated with farm group.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1231-1236
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• 2005

Genetic parameters for first lactation milk production based on test day (TD) records of 56319 Iranian Holstein cows from 655 herds that first calved between 1991 and 2001 were estimated with restricted maximum likelihood method under an Animal model. Traits analyzed were milk yield and milk fat percentage. Heritability for TD records were highest in second half of the lactation, ranging from 0.11 to 0.19 for milk yield and 0.038 to 0.094 for milk fat percentage respectively. Estimates for lactation records for these traits were 0.24 and 0.26 respectively. Genetic correlations between individual TD records were high for consecutive TD records (>0.9) and decreased as the interval between tests increased. Estimates of genetic correlations of TD yield with corresponding lactation yield were highest (0.78 to 0.86) for mid-lactation (TD3 to TD8). Phenotypic correlations were lower than corresponding genetic correlations, but both followed the same pattern. For milk fat percentage no clear pattern was found. Results of this study suggested that TD yields especially in mid-lactation may be used for genetic evaluation instead of 305-day yield.

• 종양간호연구
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• 제5권2호
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• pp.146-158
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• 2005

Purpose: This study was to obtain a understanding of breast cancer women with high risk for hereditary cancer syndrome. Method: A micro-ethnography was used, including participation observation, open-ended in-depth interviews. Results: Two major arguments were derived. First, When Korean women at high risk to hereditary breast cancer make a decision about whether to take a genetic test, they are strongly motivated by a desire to preserve close kinship bonds and "family love" among their siblings, parents and children. Second, Even after genetic risk assessment and counseling services, Korean women at high-risk for developing a hereditary breast cancer who have been informed that they are mutation carriers, still hold onto previous beliefs about cancer causation. Their cancer prevention strategies are constructed according to their unchanged perceptions and beliefs about cancer causation. Conclusion: More sensitive genetic counseling program needs to be developed. Referral programs and clinical services must be attentive to cultural values and beliefs otherwise cultural attitudes and practices toward genetic counseling will not change.

• 한국정보통신학회논문지
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• 제20권8호
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• pp.1415-1421
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• 2016

무기체계의 기능 및 성능 검증을 위한 시험들이 지속적으로 증가함에 따라 가용 자원들의 효율적인 활용을 위한 방안에 관련된 연구가 대두되고 있으며, 자원할당 복잡도가 증가함에 따라 시험계획 시에 의사결정 지원이 요구되고 있다. 시험자원할당은 전통적인 FJSP(Flexible Job Shop Problem)와 기본적으로 동일한 문제이며, 이는 NP-hard문제로서 기존의 경험기반 시험자원 할당 방법으로는 시간 효율적인 자원할당에 있어서 한계가 존재한다. FJSP에 유전자알고리즘을 적용한 최적해 탐색 연구가 진행되어 왔지만, 하나의 기계조작에 대해 두 개 이상 기계의 동시 작동이 필요한 시험자원할당 도메인에서의 적용은 제한적이다. 이에 본 논문에서는 가변 그룹 유전자알고리즘을 제안한다. 제안하는 알고리즘은 수작업 기반의 기존 시험자원할당을 자동화하고 최적화함으로써 시험 효율을 향상시킬 것으로 기대되며, MATLAB을 이용한 시뮬레이션을 통해 그 적용성을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.315-320
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• 2002

Test-day records (n=13677) sampled from 896 ewes in 5-9 (${\mu}$=7.5) monthly test-days were used to estimate genetic and phenotypic parameters of test-day yields, lactation milk yield (TMY), length of the milking period (DAYS) and three measures of persistency of milk yield in Boutsico dairy sheep. Τhe measures of persistency were the slope of the regression line (${\beta}$), the coefficient of variation (CV) of the test-day milk yields and the maximum to average daily milk yield ratio (MA). The estimates of variance components were obtained under a linear mixed model by restricted maximum likelihood. The heritability of test-day yields ranged from 0.15 to 0.24. DAYS were found to be heritable ($h^2$=0.11). Heritability estimates of ${\beta}$, CV and MA were 0.15, 0.13, 0.10, respectively. Selection for maximum lactation yields is expected to result in prolonged milking periods, high rates of decline of yields after peak production, variable test-day yields and higher litter sizes. Selection for flatter lactation curves would reduce lactation yields, increase slightly the length of the milking period and decrease yield variation as well as litter size. The most accurate prediction of TMY was obtained with a linear regression model with the first five test-day records.

• Toxicological Research
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• 제21권2호
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• pp.99-105
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• 2005

Photo-mutagenic compounds have been known to alter skin cancer rates by acting as initiators or by affecting subsequent steps in carcinogenesis. The objectives of this study are to investigate the utility of photo-chromosomal aberration (photo-CA) assay for detecting photo-clastogens, and to evaluate its ability to predict rodent photocarcinogenicity. Photo-CA assay was performed with five test substances that demonstrated positive results in photo-carcinogenicity tests: 8-Methoxypsoralen (photoactive substance that forms DNA adducts in the presence of ultraviolet A irradiation), chlorpromazine (an aliphatic phenothiazine an alpha-adrenergic blocking agent), lomefloxacin (an antibiotic in a class of drugs called fluoroquinolones), anthracene (a tricyclic aromatic hydrocarbon a basic substance for production of anthraquinone, dyes, pigments, insecticides, wood preservatives and coating materials) and Retinoic acid (a retinoid compound closely related to vitamin A). For the best discrimination between the test substance-mediated genotoxicity and the undesirable genotoxicity caused by direct DNA absorption, a UV dose-response of the cells in the absence of the test substances was firstly analyzed. All 5 test substances showed a positive outcome in photo-CA assay, indicating that the photo-CA test is very sensitive to the photo-genotoxic effect of UV irradiation. With this limited data-set, an investigation into the predictive value of this photo-CA test for determining the photo-carcinogenicity showed that photo-CA assay has the high ability of a test to predict carcinogenicity. Therefore, the photo-CA test using mammalian cells seems to be a sensitive method to evaluate the photo-carcinogenic potential of new compounds.

• 한국산림과학회지
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• 제94권2호통권159호
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• pp.73-81
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• 2005

This study was conducted to examine the geographic variation among provenances of Pinus densiflora in survival rate and height growth at four test plantations (Jungsun, Chungju, Naju, and Jeju). The plantations were parts of the eleven provenance trials of Pinus densiflora established by Korea Forest Research Institute in 1996. The survival rate and height growth were significantly different among test plantations at $p{\leq}0.01$. Latitude and longitude of test plantation were negatively correlated with survival rate and height growth. On the other hand, annual mean temperature, mean temperature (Nov.~Feb.), extremely low temperature (Dec.~Feb.), and annual mean growing days of test plantation were positively correlated with these two. The relationships between growth variables and geographic variables were analysed with canonical correlation analysis. A considerable amount of variation in survival rate and height growth was explained by latitude, annual mean growing days, extremely low temperature (Dec.~Feb.) and extremely high temperature (Nov.~Feb.) of provenances. It is estimated that up to 47.1% and 67.4% of the genetic variability in survival rate and height growth was attributable to the environmental variability of the provenances, respectively. The response surface curve of survival rate and height growth was plotted against latitude and longitude to examine growth performance of provenances for each test site. Generally, the local provenances showed better survival rate and height growth.

• Clinical and Experimental Pediatrics
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• 제53권3호
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• pp.273-285
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• 2010

Completion of the human genome project has allowed a deeper understanding of molecular pathophysiology and has provided invaluable genomic information for the diagnosis of genetic disorders. Advent of new technologies has lead to an explosion in genetic testing. However, this overwhelming stream of genetic information often misleads physicians and patients into a misguided faith in the power of genetic testing. Moreover, genetic testing raises a number of ethical, legal, and social issues. Diagnostic genetic tests can be divided into three primary but overlapping categories: cytogenetic studies (including routine karyotyping, high-resolution karyotyping, and fluorescent in situ hybridization studies), biochemical tests, and DNA-based diagnostic tests. DNA-based testing has grown rapidly over the past decade and includes preandpostnatal testing for the diagnosis of genetic diseases, testing for carriers of genetic diseases, genetic testing for susceptibility to common non-genetic diseases, and screening for common genetic diseases in a particular population. Theoretically, once a gene's structure, function, and association with a disease are well established, the clinical application of genetic testing should be feasible. However, for routine applications in a clinical setting, such tests must satisfy a number of criteria. These criteria include an acceptable degree of clinical and analytical validity, support of a quality assurance program, possibility of modifying the course of the diagnosed disease with treatment, inclusion of pre-and postnatal genetic counseling, and determination of whether the proposed test satisfies cost-benefit criteria and should replace or complement traditional tests. In the near future, the application of genetic testing to common diseases is expected to expand and will likely be extended to include individual pharmacogenetic assessments.

• 한국콘텐츠학회논문지
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• 제18권2호
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• pp.47-56
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• 2018

본 연구는 국내 외의 종양유전간호 관련 연구를 분석하여 연구동향을 파악하고자 하였다. 종양유전간호 관련 주제로 학술지에 게재된 논문들의 초록에서 제시한 핵심어들을 중심으로 한 텍스트 네트워크 분석을 실시하였다. 핵심어이자 중심성이 높은 주제어로 Nurse, Cancer, Genetic, Patient, Knowledge, Care, Genetic Test 등이 확인되었으며, 시기별 연구동향을 확인한 결과, 2003년 이후 Information, Care, Knowledge 등의 주제어를 포함한 연구들이 증가하였다. 간호학의 메타 패러다임으로 주제어를 분류한 결과, 건강, 간호, 인간, 환경 순으로 중심성이 높게 나타났다. 건강 영역 중 건강 위험 범주에서 Genetics, Risk, 건강 증진 범주에서 Genetic Test, Prevention 등이 가장 높은 빈도로 나타났다. 본 연구를 통해 종양유전간호 연구의 동향을 파악할 수 있으며, 유전성 암 환자들을 위한 간호 중재에 주축이 되는 간호사의 역할 및 중재프로그램 개발의 방향 설정에 활용될 수 있다는 점에서 의미가 있다.