• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1226-1230
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• 2005

The present investigation was undertaken to study the genetic polymorphism of the DRB3 exon 2 in 75 crossbred cattle by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Five genotypes i.e. HaeIII-a, HaeIII-b, HaeIII-e, HaeIII-ab and HaeIII-ae were observed when the 284 bp PCR products were digested with HaeIII restriction enzyme. The corresponding frequencies of these patterns were 0.53, 0.04, 0.01, 0.38 and 0.04, respectively. Digestion with RsaI restriction enzyme resolved 24 different restriction patterns. The frequencies of these patterns ranged from 0.013 (RsaI-f, RsaI-k and RsaI-c/n) to 0.120 (RsaI-n). The results revealed that the crossbred cows belonged to the RsaI patterns namely b, k, l, a/l, d/s, l/n, l/o and m/n, whose corresponding frequencies were 0.027, 0.013, 0.040, 0.027, 0.040, 0.067, 0.027 and 0.067, respectively. Digestion of the 284 bp PCR product of DRB3.2 gene with PstI in the crossbred cattle did not reveal any restriction site. These results suggested the absence of the recognition site in some of the animals. These results also revealed that the crossbred cows studied were in homozygous as well as heterozygous condition. On the basis of the above results it can be concluded that the DRB3.2 gene was found to be highly polymorphic in the crossbred cattle population.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.43-43
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• 2018

Mutation breeding by radiation is useful for improving various crop species. Up to now, a total of 170 soybean mutant varieties have been released in the world, which is the second most registered varieties after rice. Despite the economic importance of soybean, there have been no TRAP marker system studies on genetic relationships between/among mutant lines. To develop a strategy of Mutant Diversity Pool (MDP) conservation, a study on the genetic diversity of 210 soybean mutant lines (8 cultivars and 202 mutants) was performed through a TRAP analysis. Sixteen primer combinations amplified a total of 551 fragments. The highest (84.00%) and lowest (32.35%) polymorphism levels were obtained with primers MIR157B + Ga5 and B14G14B + Ga3, respectively. The mean PIC values 0.15 varied among the primer combination ranging from 0.07 in B14G14B + Sal2 to 0.23 in MIR157B + Sa4. Phylogenetic, principal component analysis (PCA) and structure analysis indicated that the 210 lines belong to four groups based on the 16 combination TRAP markers. AMOVA showed 21.0% and 79.0% variations among and within the population, respectively. Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. Our results suggest that the TRAP marker system may be useful for assessing the genetic diversity among soybean mutants and help to improve our knowledge of soybean mutation breeding.

• 대한본초학회지
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• 제28권5호
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• pp.1-6
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• 2013

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

• 생명과학회지
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• 제17권5호
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• pp.699-705
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• 2007

말 6개 품종 192두를 대상으로 17개의 microsatellite DNA marker를 이용하여 유전자(DNA)형을 분석하여 비교한 결과 제주마에서 각 marker별로 대립유전자의 수는 5-10개(평균 7.35개)로 분포하였고 제주마에서 관찰된 대립유전자는 총 125개가 관찰되어 평균 좌위 당 7.35개로서 몽고마의 130개(평균 7.65개)보다는 낮은 수치였다. 또한 AHT5 marker에서 대립유전자 P, ASB23 marker에서 대립유전자 Q와 R, CA425 marker에서 대립유전자 H, HMS3 marker에서 대립유전자 S, HTG10 marker에서 대립유전자 J, LEX3 marker에서 대립유전자 J 등 6개 marker에서 7개의 특이 대립유전자가 관찰되었다. 관찰된 이형접합성(observed heterozygosity)과 기대된 이형접합성(expected heterozygosity)은 각각 0.429-0.905(평균 0.703)와 0.387-0.841(평균 0.702)로 관찰되었고 다량정보량(PIC)은 0.354(HTG6)-0.816(LEX3)로서 평균 0.659로 나타났으며 17개 marker중 AHT4, AHT5, CA425, HMS2, HMS3, HTG10, LEX3, VHL20 marker 등이 다량정보량(PIC) 0.7 이상을 나타내었다. 17개 marker에 대한 전체 부권부정율(친부마 혹은 친모마 하나의 유전자형을 알고 있을 경우)을 제주마에 적용 시 99.99%로 나타났다. 말 6개 품종별로 분석하였을 때 평균 대립유전자의 수는 7.64개(몽고마)-4.23개(미니츄어 말)로 분포하였고 17개 marker 전체에서는 153개의 대립유전자가 검출되었다. 품종별로 분석한 결과 기대된 이형접합성(expected heterozygosity)과 관찰된 이형접합성(observed heterozygosity)은 각각 0.7950$\pm$0.0141(몽고마)-0.6751$\pm$0.0378(미니츄어 말), 0.7135$\pm$0.0180(제주경주마)-0.5621$\pm$0.0401(미니츄어 말)로 나타났다. 말 6개 품종을 17개 microsatellite marker로 분석한 결과 몽고마, 제주마, 제주경주마 등의 순으로 높은 유전적 다양성을 보였다. 제주마와 가장 가까운 유전적 유연 관계를 나타낸 집단은 몽고마로서 Da genetic distance에서 0.1517로 나타났고, 제주경주마와는 0.2628의 유전적 거리를 보였다.

• 한국가금학회지
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• 제23권4호
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• pp.177-183
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• 1996

This study was conducted to analyze genetic characteristics of Korean Native Chicken three lines classified on the basis of the feather color and appearance (Red, Yellow, and Black) using DNA fingerprinting method. To estimate the genetic relatedness among breeds and similarities within breeds, we collected blood samples from Korean Native Chicken (KNC), Rhode Island Red (RIR), White Leghorn (WL), and Cornish(CN) and obtained genomic DNA from the blood of 10 individuals randomly selected within the breeds and lines. The genomic DNA samples were digested with restriction enzymes (Hinf J, Hae Ill) and hybridized with various probes (Jeffreys' probes 33.15, 33.6 and M13) after Southern transfer. Genetic similarities within breeds were characterized by band sharing (BS) value, estimated by the DFP band pattern between the pair of lanes. BS values within WL, RIR, and KNC were 0.82, 0.70 and 0.56, respectively. Relative genetic diversity (BS value) of KNC was higher than those two breeds (WL, RIR). Estimation of genetic similarity between KNC lines and control breed (RIR) was 0.32, whereas similarity within KNC lines (6 groups) was 0.50. In this analysis, KNC was showed to have a highly genetic diver-sity at the DNA level, and to be closer in genetic distance to RIR (0.67) than any other breeds.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.110-110
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• 2022

Rice is the world's most important food crop and a major source of nutrition for about two thirds of populations. Northern Vietnam is one of the most important centers of genetic diversity for cultivated rice. In this study, we determined the genetic diversity and population structure of 79 rice landraces collected from northern Vietnam and 19 rice accessions collected from different countries. In total, 98 rice accessions could be differentiated into japonica and indica with moderate genetic diversity and a polymorphism information content of 0.382. We also detected subspecies-specific markers to classify rice (Oryza sativa L.) into indica and japonica. Additionally, we detected five marker-trait associations and rare alleles that can be applied in future breeding programs. Most interestingly, analysis of molecular variance (AMOVA) found genetic differentiation was related to geographical regions with an overall PhiPT (analog of fixation index FST) value of 0.130. More emphasis was given to provide signatures and infer explanations about the role of geographical isolation and environmental heterogeneity in genetic differentiation among regions in landraces from northern Vietnam. Our results suggest that rice landraces in northern Vietnam have a dynamic genetic system that can create different levels of genetic differentiation among regions, but also maintain a balanced genetic diversity between regions.

• 한국가금학회지
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• 제42권1호
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• pp.15-26
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• 2015

Microsatellite(MS) marker는 가축의 유전적 특성 및 계통간의 유연관계 분석에 있어 많이 이용되고 있는 분자유전학적 유전표지로 유전체 상에 널리 분포하고 있으며, 높은 변별력과 검출이 간편하다. 본 연구는 한협 8개 계통과 토종닭 순계 12 계통을 대상으로 27종 MS marker의 유전자형을 분석하고, 이를 기반으로 유전적 특성 및 계통간 유연관계를 구명함으로써 한협에서 생산되고 있는 토종닭 계통들의 유전적 배경이나 특성에 대한 기초자료를 제공하고자 실시하였다. 연구 결과, 27종 MS marker 분석 결과, 평균 11.7개의 대립유전자가 확인되었으며, $H_{exp}$와 PIC의 경우 MCW0288이 각각 0.688, 0.646으로 가장 낮았고, MCW0104가 0.881, 0.869로 가장 높게 확인되었으며, 집단별 $F_{is}$(f)값을 통해 한협 H 계통(HH)은 집단 내 개체 간 상관 정도가 가장 적은 것으로 확인되었다. 계통 간 유연관계를 분석한 결과, 전체 20계통 중 로드 아일랜드 레드 계통(NC와 ND)이 가장 가까운 것(0.175)으로 확인되었으며, 반면에 레그혼 F(NF)와 로드 아일랜드 레드 D(ND) 계통이 가장 먼 것(0.710)으로 확인되었다. MS marker의 효율성을 검증하기 위해 동일개체 출현확률(PI)을 분석한 결과, 전체 계통에 대한 동일개체 출현확률(PI)과 한협 8계통의 동일개체 출현확률(PI)은 27종의 MS marker를 사용하였을 경우, 각각 $8.80{\times}10^{-83}$, $3.87{\times}10^{-117}$으로 확인되었다. 따라서 본 연구는 토종닭 시장에서 높은 점유율을 보유한 한협의 실용계 계통의 유전적 배경과 특성분석을 통해 향후 소비자의 요구에 부합하는 토종닭의 개량 및 실용계 계통의 개발을 위한 기초자료로 유용하게 활용될 것으로 기대된다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2002년도 추계 수산관련학회 공동학술대회발표요지집
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• pp.183-184
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• 2002

The use of transgenic fish has so far been chiefly limited by the lack of predictable, strong, tissue specific, and position-independent expression of transgenes. For genetic analysis, expression of a marker transgene, easily screenable in the living fish, could facilitate studies of gene targeting, insertional mutagenesis, lineage, and mutational analysis. (omitted)

• 한국가금학회지
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• 제37권4호
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• pp.355-360
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• 2010

본 연구는 국내에 보급되고 있는 대표적인 토종닭 브랜드인 한협3호와 농촌진흥청에서 개발된 브랜드인 우리맛닭, 두 브랜드 집단 간의 유전적 특성을 분석하여 향후 브랜드간의 차별화 전략을 구체화 하는데 있어 기초 자료로 활용하고자 실시하였다. 토종닭 실용계인 우리맛닭(W) 152수와 한협3호(H) 150수, 총 302수를 선발하여 공시재료로 활용하였으며, 다형성이 확인된 10종의 MS marker를 선발하여 활용하였다. 분석 결과, 두 브랜드의 평균 대립유전자의 수는 9.3으로 확인되었으며, 우리맛닭의 평균 대립유전자의 수는 8.4개, 한협3호는 7.2개로 우리맛닭이 보유한 대립유전자의 수가 많은 것으로 확인되었다. 반면, 기대되는 이형접합도(Ex H)와 관측된 이형접합도(Ob H)는 한협3호가 우리맛닭에 비해 높은 것으로 확인되었다. 두 브랜드 집단을 대상으로 각각의 MS marker의 유전자형을 분석하여 브랜드별 기대되는 이형접합도(expected heterozygosity: Ex H)와 관측된 이형접합도(observed heterozygosity: Ob H) 및 PIC(polymorphism information content)값을 계산하였다. 두 브랜드 집단 간의 유전적 유연관계를 분석 결과, DA distance는 0.132, 그리고 standard genetic distance는 0.199로 확인되었다. 두 브랜드 집단 간의 유전적 구조에 따라 각 개체들이 어떻게 분포되어 있는가를 확인한 결과, 두 집단에 속한 개체들은 크게 두 개의 그룹으로 나뉘어 분포하고 있음을 확인할 수 있었다. 두 집단 간의 유전적 거리는 비교적 가깝지만 각 개체들간의 유전적 구조를 분석한 결과, 확연히 구분되는 유전적 특성을 지니고 있음을 확인하였다.

• 한국작물학회지
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• 제45권2호
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• pp.123-127
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• 2000

The objective of this study was to develop a linkage map of soybean under the genetic background of Korean soybean. A set of 89 F/sub 5/ lines was developed from a cross between 'Pureunkong', which was released for soy-bean sprout, and 'Jinpumkong 2', which had no beany taste in seed due to lack of lipoxygenase 1, 2, and 3. A linkage map was constructed for this population with a set of 113 genetic markers including 7 restriction fragment length polymorphism (RFLP) markers, 79 randomly amplified polymorphic DNA (RAPD) markers, 24 simple sequence repeat(SSR) markers, and 3 morphological markers. The map defined approximately 807.4 cM of the soybean genome comprising 25 linkage groups with 98 polymorphic markers. Fifteen markers remained unlinked. Seventeen linkage groups identified here could be assigned to the respective 13 linkage groups in the USDA soybean genetic map. RFLP and SSR markers segregated at only single genetic loci. Fourteen of the 25 linkage groups contained at least one SSR marker locus. Map positions of most of the SSR loci and their linkages with RFLP markers were consistent with previous reports of the USDA soybean linkage groups. For RAPD, banding patterns of 13 decamer primers showed independent segregations at two or more marker loci for each primer. Only the segregation at op Y07 locus was expressed with codominant manner among all RAPD loci. As the soybean genetic map in our study is more updated, molecular approaches of agronomically important genes would be useful to improve Korean soybean improvement.