• Preventive Nutrition and Food Science
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• v.1 no.1
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• pp.87-92
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• 1996

This study describes a stable and simple method for the measurement of cholesteryl ester transfer protein(CETP) activities using reconstituted HDL and LDL as substrates. Apolipoproteins (apo) A-I and -B were purified from hog plasma by a new strategy without ultracentrifugation and delipidation. a simple two-step column chromatography was administered. In the first step of phenyl-sepharose CL-4B column chro-matography, hydrophobic plasma proteins were isolated. The most hydrophobic proteins bound to the column appeared to be A-I and apo-B. Contaminat proteins were efficiently eliminated from the sample by washing the column with 0.3M NaCI containing buffer after loading the plasma on the column. Two pure proteins showing each single band on SDS-PSGE of apo A-I and apo-B were individually obtained by a subsequent gel filtration column chromatography(Sephadex G-200). This two-step purification was simple and inexpensive compared to the ultracentrifugation and/or delipidation method that are most commonly used. Reconstituted hight-density lipoproteins(HDL) and low-density lipoproteins(LDL) were prepared using the purified apo A-I and-B, respectively. When these artificially prepared HDL and LDL were used in the assays for CETP as the cholesteryl ester(CE) donor and acceptor respectively, the specific transfer of CE increased up to two fold compared to that used the native HSL and LDL.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.16 no.2
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• pp.86-92
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• 2016

Purpose: The $BH_4$ loading test is an important test that distinguishes PKU from $BH_4$ deficiency and identifies the $BH_4$ reactivity of PKU patients. Phenylalanine and $BH_4$ loading tests are useful methods that can shorten the length of hospital stay while improving patients' convenience. However, sufficient research on the dose of phenylalanine loading and $BH_4$ administration time after the loading has not been carried out. The present study investigates the effectiveness of the existing phenylalanine loading method by analyzing the medical records of six patients who underwent the $BH_4$ loading test after taking 100 mg/kg of phenylalanine patients. Methods: The medical records of six patients who underwent the $BH_4$ load test after taking 100 mg/kg of phenylalanine were examined out of 207 patients who were followed up in the Genetic Metabolic Clinic in Soonchunhyang University Hospital. All of the six patients had a low phenylalanine diet. First, they were taking 100 mg/kg of phenylalanine. 3 hours later, 20 mg/kg of $BH_4$ were loaded. The phenylalanine levels in the blood were continuously measured at 1, 2, 4, 6, 8, 12, and 24 hours by setting the time the $BH_4$ was loaded as the basal. Results: The average of the highest phenylalanine concentrations of six patients was $20.0{\pm}11.70mg/dL$. One reached the highest concentration seven hours after taking phenylalanine; another reached it five hours after that, and the remaining three reached it four hours after that. Only one patient reached the highest concentration within three hours. The phenylalanine levels of four out of six patients (66%) rose above $400{\mu}mol/L$ after being loaded with phenylalanine. The phenylalanine levels of the remaining two were 6.1 mg/dL ($366{\mu}mol/L$) and 5 mg/dL ($300{\mu}mol/L$), respectively. Conclusion: One of six patients (16%) reached the highest concentration three hours after taking 100 mg/kg of phenylalanine and four patients (66%) reached $400{\mu}mol/L$ or higher phenylalanine levels. There were patients whose phenylalanine levels did not rise above $400{\mu}mol/L$ using a commonly known test method; moreover, this method had the disadvantage of reaching the highest concentration after more than three hours. Therefore, it is considered that taking 200 mg/kg or more of phenylalanine and performing $BH_4$ loading four to six hours after taking phenylalanine are helpful in proper diagnosis.

• Journal of Korean Society of Forest Science
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• v.97 no.4
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• pp.431-436
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• 2008

7 populations of rare endemic Berchemia berchemiaefolia were analysed using multivariate analysis for 9 characteristics of leaf morphology. The aim of this study was to examine geographic variation to support a genetic resource conservation plan of B. berchemiaefolia in Korea. In the morphological characters, nine characters of leaf were 10.25 cm (blade length), 4.10 cm (maximum width), 2.52 (blade length/maximum width), 3.22 cm (upper 1/3 width), 3.42 cm (lower 1/3 width), 0.95 (upper 1/3 width/lower 1/3 width), 1.24 cm (petiole length), 8.91 (blade length/petiole length), 8.16ea (vein number), respectively. Nested anova showed that were statistically significant differences among populations as well as among individuals within populations in all 9 quantitative characters. In 7 of 9 characters, variance components among individuals within populations were higher than those among populations. Cluster analysis using complete linkage method showed two groups (Chungbuk and Gyeongbuk districts) to Euclidean distance 1.2. Among principal components, primary 3 principal components appeared to be major variables because of the loading contribution of 87.3%. The first contribution was blade length, blade length/maximum width and blade length/petiole length; the second one was maximum width, upper 1/3 width and lower 1/3 width; the third one was petiole length, respectively.

• Plant Resources
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• v.2 no.2
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• pp.75-80
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• 1999

Twenty two varieties of pecan including wild types were classified based on 6 characters measured by principal component analysis score distance. The results are summarized as fellow. Twenty two varieties were classified into 5 groups based in PCA score distance. Five groups were distinctly characterized by many morphological characters. Total variation could be explained by 51%, 95%, 99% with first, third and fifth principal components respectively. Varimax rotation of the factor loading of the first factors indicated that the first component was highly loaded with leaf characters, the second component with fruit characters, but fruit length was negative loaded. The second, the third and the fourths groups of cultivars had very close genetic parentage similarity.

• Proceedings of the KSR Conference
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• 2004.10a
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• pp.579-584
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• 2004

In development of the bogie, the fatigue strength of the bogie frame is an important design criteria. Also the bogie frame weight reduction is required in order to save energy and materials. In this study. structural analysis of bogie frame by using the finite element method has been performed for the various loading conditions according to the UIC standards and it has been attempted minimize the weight of bogie frame by back-propagation neural network and genetic algorithm. Finite element mesh generation and finite element analysis were performed by Altaire Hyper Mesh and ABAQUS.

• Journal of Electrical Engineering and Technology
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• v.4 no.1
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• pp.19-27
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• 2009

This paper presents a newly developed optimization algorithm, the Bee Optimization Algorithm (BeeOA), to solve the economic power dispatch (EPD) problem. The authors have developed a derivative free and global optimization technique based on the working of the honey bee. The economic power dispatch problem is a nonlinear constrained optimization problem. Classical optimization techniques fail to provide a global solution and evolutionary algorithms provide only a good enough solution. The proposed approach has been examined and tested on two test systems with different objectives. A simple power dispatch problem is tested first on 6 generators and then the algorithm is demonstrated on 13 thermal unit systems whose incremental fuel cost function takes into account the value point loading effect. The results are promising and show the effectiveness and robustness of the proposed approach over recently reported methods.

• Proceedings of the KOR-KST Conference
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• 2002.02a
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• pp.45-77
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• 2002

교통혼잡 문제가 점점 심각해짐에 따라 대중교통의 중요성은 날로 부각되며, 대중교통을 지원하기 위한 정책들이 속속 입안되고 있어 대중교통을 심도 있게 분석할 수 있는 틀의 개발은 필연적이라 할 수 있다. 이에 본 연구는 대중교통 통행배정모형 개발과 대중교통 기종점통행량(OD) 추정을 목적으로 수행되었다. 대중교통 통행배정모형의 개발부분에서는 기존의 대중교통 통행배정모형이 개별차량과 다른 대중교통의 특성을 정확히 반영하고 있지 못하다는 한계를 극복하고자, 최적경로 탐색에는 유전자 알고리즘(Genetic Algorithm)을 통행량 배정에는 로짓모형을 기반으로 한 확률적 통행량 배정모형(Stochastic Network Loading Model)을 이용하여 TATSN 모형을 개발하였다. 그리고, 대중교통 기종점통행량의 추정은 전통적인 기종점통행량 추정 방법인 기종점조사 방법이 시간과 비용이 과대하게 소요된다는 단점을 인식하여 관측통행량을 이용하여 추정하는 방법을 제안하였다.

• Steel and Composite Structures
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• v.31 no.5
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• pp.427-435
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• 2019

The estimation of moment and rotation in steel rack connections could be significantly helpful parameters for designers and constructors in the initial designing and construction phases. Accordingly, Extreme Learning Machine (ELM) has been optimized to estimate the moment and rotation in steel rack connection based on variable input characteristics as beam depth, column thickness, connector depth, moment and loading. The prediction and estimating of ELM has been juxtaposed with genetic programming (GP) and artificial neural networks (ANNs) methods. Test outcomes have indicated a surpass in accuracy predicting and the capability of generalization in ELM approach than GP or ANN. Therefore, the application of ELM has been basically promised as an alternative way to estimate the moment and rotation of steel rack connection. Further particulars are presented in details in results and discussion.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.32 no.6
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• pp.383-390
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• 2019

A helideck is one of the essential structures in offshore platforms for the transportation of goods and operating personnel between land and offshore sites. As such, it should be carefully designed and installed for the safety of the offshore platform. In this study, a structural design optimization method for a lightweight offshore helideck is developed based on a genetic algorithm and an attainable design set concept. A helideck consists of several types of structural members such as plates, girders, stiffeners, trusses, and support elements, and the dimensions of these members are typically pre-defined by manufacturers. Therefore, design sets are defined by collecting the standard section data for these members from the American Institute of Steel Construction (AISC), and integer section labels are assigned as design variables in the genetic algorithm. The objective is to minimize the total weight of the offshore helideck while satisfying the maximum allowable stress criterion under various loading conditions including self-weight, wind direction, landing position, and landing condition. In addition, the unity check process is also utilized for additional verification of structural safety against buckling failure of the helideck.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.12
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• pp.4915-4918
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• 2014

Background and Aims: Advances in the treatment of cervical cancer over the last decade have predominantly involved the development of genes directed at molecular targets. Gene therapy is recognized to be a novel method for the treatment of cervical cancer. Genes can be administered into target cells via nanocarriers. This study aimed to develop systemically administrable nano-vectors. Floate (Fa) containing gene loaded nanoparticles (NPs) could target HeLa human cervical cancer cells through combination with receptors on the cells to increase the nuclear uptake of genetic materials. Methods: Fa was linked onto Poly (ethylene glycol)-b-poly (D, L-lactide) (PEG-PLA) to form Fa-PEG-PLA, and the resulting material was used to load plasmids of enhanced green fluorescence protein (pEGFP) to obtain gene loaded nanoparticles (Fa-NPs/DNA). Physical-chemical characteristics, in vitro release and cytotoxicity of Fa-NPs/DNA were evaluated. The in vitro transfection efficiency of Fa-NPs/DNA was evaluated in HeLa cells and human umbilical vein endothelial cells (HUVEC). PEG-PLA without Fa was used to load pEGFP from NPs/DNA as a control. Results: Fa-NPs/DNA has a particle size of 183 nm and a gene loading quantity of 92%. After 72h of transfection, Fa-NPs/DNA displayed over 20% higher transfection efficiency than NPs/DNA and 40% higher than naked DNA in HeLa cells. However, in HUVECs, no significant difference appeared between Fa-NPs/DNA and NPs/DNA. Conclusions: Fa-PEG-PLA NPs could function as excellent materials for gene loading. This nano-approach could be used as tumor cell targeted medicine for the treatment of cervical cancer.