• Title/Summary/Keyword: Genetic interaction

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A comparative study of carcass characteristics and meat quality in genetic resources Pekin ducks and commercial crossbreds

  • Kokoszynski, Dariusz;Piwczynski, Dariusz;Arpasova, Henrieta;Hrncar, Cyril;Saleh, Mohamed;Wasilewski, Rafal
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.11
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    • pp.1753-1762
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    • 2019
  • Objective: The study was aimed to compare carcass traits, physicochemical and textural properties of meat in two different genotypes of Pekin ducks with regard to sex effect. Methods: The study involved 120 Pekin ducks: 30 males and 30 females of strain P33 (Polish native Pekin ducks) and 30 males and 30 females of Star 53 HY (commercial hybrid Pekin ducks). At 49 d of age, 48 birds (12 males and 12 females of each genotype) were selected for dissection. After the dissection, meat samples were collected to determine meat quality traits. Results: The studied Pekin ducks of different genotype showed significant differences in body weight, carcass weight, dressing percentage, as well as percentages of breast muscles, skin with subcutaneous fat, abdominal fat, neck, and remainders of eviscerated carcass with neck. Duck genotype influenced the content of crude protein, crude fat, Na, K, P, Zn, $pH_{24}$, electric conductivity ($EC_{24}$), cooking loss, $L^{\star}$, $a^{\star}$, most textural traits of breast muscle, and also Na, Mg and Fe content, $EC_{24}$, drip loss, cooking loss and $L^{\star}$, $a^{\star}$, and $b^{\star}$ colour coordinates of leg muscles. Regardless of genetic origin, males exhibited higher BW, carcass weight and carcass neck percentage, as well as lower redness, hardness, chewiness and gumminess of breast muscle compared to females. The genotype${\times}$sex interaction was significant for the crude fat content and cooking loss of breast muscle, and for the yellowness of leg muscle. Conclusion: Star 53 HY ducks are more suited for broiler production due to their higher body weight and dressing percentage. Their breast and leg meat are characterized by more beneficial chemical composition but has poorer sensory and textural properties compared to the meat of P33 ducks.

Physiochemical characteristics and fermentation ability of milk from Czech Fleckvieh cows are related to genetic polymorphisms of β-casein, κ-casein, and β-lactoglobulin

  • Kyselova, Jitka;Jecminkova, Katerina;Matejickova, Jitka;Hanus, Oto;Kott, Tomas;Stipkova, Miloslava;Krejcova, Michaela
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.1
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    • pp.14-22
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    • 2019
  • Objective: The aim of the study was to find a possible association between the ${\beta}-$ and ${\kappa}-casein$ and ${\beta}-lactoglobulin$ genotypes and important milk physiochemical and technological characteristics such as acidity, alcohol stability, the contents of some minerals and the parameters of acid fermentation ability (FEA) in Czech Fleckvieh Cattle. Methods: Milk and blood samples were collected from 338 primiparous Czech Fleckvieh cows at the same stage of lactation. The genotypes of individual cows for ${\kappa}-casein$ (alleles A, B, and E) and ${\beta}-lactoglobulin$ (alleles A and B) were ascertained by polymerase chain reaction-restriction fragment length polymorphism, while their ${\beta}-casein$ (alleles $A^1$, $A^2$, $A^3$, and B) genotype was determined using melting curve genotyping analysis. The data collected were i) milk traits including active acidity (pH), titratable acidity (TA), alcohol stability (AS); calcium (Ca), phosphorus (P), sodium (Na), magnesium (Mg), and potassium (K) contents; and ii) yoghurt traits including active acidity (Y-pH), titratable acidity (Y-TA), and the counts of both Lactobacilli and Streptococci in 1 mL of yoghurt. A linear model was assumed with fixed effects of herd, year, and season of calving, an effect of the age of the cow at first calving and effects of the casein and lactoglobulin genotypes of ${\beta}-CN$ (${\beta}-casein$, CSN2), ${\kappa}-CN$ (${\kappa}-casein$, CSN3), and ${\beta}-LG$ (${\beta}-lactoglobulin$, LGB), or the three-way interaction between those genes. Results: The genetic polymorphisms were related to the milk TA, AS, content of P and Ca, Y-pH and Lactobacilli number in the fresh yoghurt. The CSN3 genotype was significantly associated with milk AS (p<0.05). The effect of the composite CSN2-CSN3-LGB genotype on the investigated traits mostly reflected the effects of the individual genes. It significantly influenced TA (p<0.01), Y-pH (p<0.05) and the log of the Lactobacilli count (p<0.05). Conclusion: Our findings indicate that the yoghurt fermentation test together with milk proteins genotyping could contribute to milk quality control and highlight new perspectives in dairy cattle selection.

Evaluation of Regional Adaptability in Introduced Super Sweet Corn Hybrids and Heritability of Agronomic Traits (도입 초당옥수수 교잡종의 지역 적응성 및 농업 형질의 유전력 평가)

  • Lee, Shin-Young;Kang, Jong-won;Wang, Seung-hyun;Park, Tai-choon;Chung, Jong-Wook;So, Yoon-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.2
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    • pp.130-137
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    • 2021
  • This study evaluated newly introduced, commercial super sweet corn hybrids (Zea mays L.) for their potential as breeding materials. Agronomic traits were measured and variance components were obtained using a linear mixed model to estimate the heritability. The trials were carried out in 2018 at two locations (Haenam and Oksan in South Korea). All traits had low heritability, except for mid tasseling and silking days. These traits with low heritability mostly had low genetic variance component estimate. In case of ear height ratio, significant genotype by location appeared to be responsible for low genetic variance, which in turn led to low heritability. Low heritability estimates from the trials with commercial hybrids were perhaps because those hybrids were highly improved for commercial success. Hence, this does not necessarily point to them having poor potential as breeding materials. To overcome low heritability, significant genotype by environment interaction, and achieve high selection efficiency, intermating among hybrids is recommended to create new recombinants before inbred line development.

Effect of Different Sowing Dates on the Yield-related Traits and Seed Yield of Sesame (파종시기에 따른 참깨 수량구성요소 및 수량에 미치는 영향)

  • Shim, Kang Bo;Shin, Myoung Na;Jeon, Won Tae;Han, Arum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.411-418
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    • 2021
  • The yield potential of sesame depends on genetic factor, environmental factor, genetic × environmental interaction, as well as agronomic factors such as sowing date, planting density or seed rate for sowing. The main aim of this study was to analyze the effects of different sowing dates on the yield-related traits and total seed weight of sesame. The analysis of variance revealed that different sowing dates affected sesame yield-related traits such as plant height, number of branch per plant, number of capsule per plant, 1,000-seed weight, and total seed weight. Early or late sowing date showed negative effect of yield-related sesame traits and total seed weight. Optimum sowing date for sesame in the middle region of Korean peninsula was May 10th, in which total seed yield recorded 90 kg per 10 are. Path-coefficient analysis revealed that the number of capsule per plant and plant height were major factors that affected the total seed weight of sesame across different sowing dates.

Evaluation and Application of QUAL2E and QUAL2K Models in Anyang Stream (안양천에서 QUAL2E와 QUAL2K 모델의 적용 및 평가)

  • Jung, Sung-Soo;Kim, Kyung-Sub
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.5
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    • pp.544-551
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    • 2008
  • QUAL2K enhanced QUAL2E and applied in real fields efficiently incorporates denitrification process, sediment-water interaction process, bottom algae and detritus. Also, the CBOD of QUAL2K is divided into two real parts, one is slow CBOD(sCBOD) and another is fast CBOD(fCBOD). The simulation results of QUAL2E and QUAL2K models in Anyang Stream were compared and analyzed in water quality constituents of DO, BOD, Org-N, NH$_3$-N, NO$_3$-N, Org-P, Dis-P and Chl-a respectively. The similar results were shown in Org-N, NH$_3$-N, Org-P and Chl-a both QUAL2K and QUAL2E models. But the different results were revealed in DO, BOD, Dis-P and NO$_3$-N by the influence of new incorporating processes. DO was shown relatively low values in the effect of bottom algae. BOD which is influenced by particulate organic matter was revealed high values. NO$_3$-N was closed to the real values by the two processes of denitrification and sediment-water interaction. To evaluate the running results of QUAL2K and QUAL2E models, a simple statistical analysis was conducted. According to the statistical analysis, QUAL2K represented less relative error and coefficient of variation than QUAL2E in almost all of constituents. It was found that QUAL2K, which simulates the water quality more realistically, can be applied to control and manage the water problems of river or river-run reservoir effectively.

The interaction of Apolipoprotein A5 gene promoter region T-1131C polymorphism (rs12286037) and lifestyle modification on plasma triglyceride levels in Japanese

  • Yamasaki, Masayuki;Mutombo, Paulin Beya wa Bitadi;Iwamoto, Mamiko;Nogi, Akiko;Hashimoto, Michio;Nabika, Toru;Shiwaku, Kuninori
    • Nutrition Research and Practice
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    • v.9 no.4
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    • pp.379-384
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    • 2015
  • BACKGROUND/OBJECTIVE: Apolipoprotein A5 gene promoter region T-1131C polymorphism (APOA5 T-1131C) is known to be associated with elevated plasma TG levels, although little is known of the influence of the interaction between APOA5 T-1131C and lifestyle modification on TG levels. To investigate this matter, we studied APOA5 T-1131C and plasma TG levels of subjects participating in a three-month lifestyle modification program. SUBJECTS/METHODS: A three-month lifestyle modification program was conducted with 297 participants (Age: $57{\pm}8years$) in Izumo City, Japan, from 2001-2007. Changes in energy balance (the difference between energy intake and energy expenditure) and BMI were used to evaluate the participants' responses to the lifestyle modification. RESULTS: Even after adjusting for confounding factors, plasma TG levels were significantly different at baseline among three genotype subgroups: TT, $126{\pm}68mg/dl$; TC, $134{\pm}74mg/dl$; and CC, $172{\pm}101mg/dl$. Lifestyle modification resulted in significant reductions in plasma TG levels in the TT, TC, and CC genotype subgroups: $-21.9{\pm}61.0mg/dl$, $-20.9{\pm}51.0mg/dl$, and $-42.6{\pm}78.5mg/dl$, respectively, with no significant differences between them. In a stepwise regression analysis, age, APOA5 T-1131C, body mass index (BMI), homeostasis model assessment-insulin resistance (HOMA-IR), and the 18:1/18:0 ratio showed independent association with plasma TG levels at baseline. In a general linear model analysis, APOA5 T-1131C C-allele carriers showed significantly greater TG reduction with decreased energy balance than wild type carriers after adjustment for age, gender, and baseline plasma TG levels. CONCLUSIONS: The genetic effects of APOA5 T-1131C independently affected plasma TG levels. However, lifestyle modification was effective in significantly reducing plasma TG levels despite the APOA5 T-1131C genotype background.

Dna2 Helicase/endonuclease Interacts with a Novel Protein YHR122W Protein in Saccharomyces cerevisiae (Saccharomyces cerevisiae에서 Dna2 helicase/endonuclease와 YHR122W 단백질의 상호작용)

  • Lee, Hyun-Sun;Choi, Do-Hee;Kwon, Sung-Hoon;Kim, Na-Yeon;Lee, In-Hwan;Kim, Hyun-Jung;Bae, Sung-Ho
    • Korean Journal of Microbiology
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    • v.42 no.1
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    • pp.1-6
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    • 2006
  • Saccharomyces cerevisiae Dna2 helicase/endonuclease plays an essential role in removing DNA primers during Okazaki fragment processing in eukaryotic DNA replication. Genome-wide scale co-immunoprecipitation experiments predicted that Dna2 interacts with a novel protein YHR122W (1). In this study, we observed that overexpression of YHR122W gene suppressed the temperature-sensitive phenotype of $dna2\Delta405N$ mutation. To investigate direct interaction between these two proteins, a histidine-tagged recombinant YHR122W protein was expressed and purified from E. coli. Physical interaction between the purified YHR122W and Dna2 proteins was detected by enzyme-linked immunosorbent assays. Further more, the complex formation was most efficient at physiological salt concentration, 150 mM NaCl. The genetic and physical interactions between YHR122W and Dna2 shown in this study suggest that the biological functions of these two proteins may be closely related each other.

CYP2W1, CYP4F11 and CYP8A1 Polymorphisms and Interaction of CYP2W1 Genotypes with Risk Factors in Mexican Women with Breast Cancer

  • Cardenas-Rodriguez, N.;Lara-Padilla, E.;Bandala, C.;Lopez-Cruz, J.;Uscanga-Carmona, C.;Lucio-Monter, P.F.;Floriano-Sanchez, E.
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.3
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    • pp.837-846
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    • 2012
  • Breast cancer (BCa) is the leading type of cancer in Mexican women. Genetic factors, such as single nucleotide polymorphisms (SNP) of P450 system, have been reported in BCa. In this report, and for the first time in the literature, we analyzed the rs3735684 (7021 G>A), rs11553651 (15016 G>T) and rs56195291 (60020 C>G) polymorphisms in the CYP2W1, 4F11 and 8A1 genes in patients with BCa and in healthy Mexican women to identify a potential association between these polymorphisms and BCa risk. Patients and controls were used for polymorphism analysis using an allelic discrimination assay with TaqMan probes and confirmed by DNA sequencing. Links with clinic-pathological characteristics were also analyzed. Statistical analysis was performed using the standard ${\chi}^2$ or Fisher exact test statistic. No significant differences were observed in the distributions of CYP2W1 (OR 8.6, 95%CI 0.43-172.5 P>0.05; OR 2.0, 95%CI 0.76-5.4, P>0.05) and CYP4F11 (OR 0.3, 95%CI 0.01-8.4 P>0.05) genotypes between the patients and controls. Only the CYP8A1 CC genotype was detected in patients with BCa and the controls. All polymorphism frequencies were in Hardy-Weinberg Equilibrium (HWE) in the controls (P>0.05). We found a significant association between BCa risk and smoking, use of oral contraceptives or hormonal replacement therapy (HRT), obesity, hyperglycemia, chronic diseases, family history of cancer and menopausal status in the population studied (P<0.05). Tobacco, oral contraceptive or HRT, chronic diseases and obesity or overweight were strongly associated with almost eight, thirty-five, nine and five-fold increased risk for BCa. Tobaco, obesity and hyperglycemia significantly increased the risk of BCa in the patients carrying variant genotypes of CYP2W1 (P<0.05). These results indicate that the CYP2W1 rs3735684, CYP4F11 rs11553651 and CYP8A1 rs56195291 SNPs are not a key risk factor for BCa in Mexican women. This study did not detect an association between the CYP2W1, 4F11 and 8A1 genes polymorphisms and BCa risk in a Mexican population. However, some clinico-pathological risk factors interact with CYP2W1 genotypes and modifies susceptibility to BCa.

Study on Interaction of Planar Redundant Manipulator with Environment based on Intelligent Control (지능제어를 이용한 평면 여자유도 매니퓰레이터와 환경과의 상호작용에 관한 연구)

  • Yoo, Bong-Soo;Kim, Sin-Ho;Joh, Joong-Seon
    • Journal of the Korean Institute of Intelligent Systems
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    • v.19 no.3
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    • pp.388-397
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    • 2009
  • There are many tasks which require robotic manipulators interaction with environment. It consists of three control problems, i.e., position control, impact control and force control. The position control means the way of reaching to the environment. The moment of touching to the environment yields the impact control problem and the force control is to maintain the desired force trajectory after the impact with the environment. These three control problems occur in sequence, so each control algorithm can be developed independently. Especially for redundant manipulators, each of these three control problems has been important independent research topic. For example, joint torque minimization and impulse minimization are typical techniques for such control problems. The three control problems are considered as a single task in this paper. The position control strategy is developed to improve the performance of the task, i.e., minimization of the individual joint torques and impulse. Therefore, initial conditions of the impact control problem are optimized at the previous position control algorithm. Such a control strategy yields improved result of the impact control. Similarly, the initial conditions for the force control problem are indirectly optimized by the previous position control and impact control strategies. The force control algorithm uses the individual joint torque minimization concept. It also minimizes the force disturbances. The simulation results show the proposed control strategy works well.

Genetic Analysis of Days to Flowering in Korean Hot Pepper (한국 건고추 개화소요일수의 유전 분석)

  • Soh, Jae-Woo;Lee, Yong-Beom;Nam, Sang-Yong
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.89-94
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    • 2013
  • This study was carried out to calculate the genic values of days for flowering in commercial breeding lines of Korean hot pepper (Capsicum annuum L.). Two breeding lines of pepper '#2132' ($P_1$) early-flowering, and '#1308' ($P_2$) medium-late flowering, and their $F_1$ and $F_2$ generations were used in this study. By using partitioning method (Thseng and Hosokawa, 1971, 1972), it was possible to estimate, from the $F_2$ generations, the number of effective factor pairs differentiating the two parents. It was found out that the two parents were differentiated by two effective factor pairs, A:a and B:b. In the breeding lines used, the inheritance of days to flowering showed that the $F_1$ flowered a little earlier than the earlier flowering parent through the effect of over-dominance. However, $F_2$ flowered earlier or later than both parents through transgressive segregation. Conclusively, the magnitude of genic effects of A-a gene in flowering days was -13.81 days, and B-b gene was -6.73 days. The interaction between the two non-allelic factors using partitioning method was -5.26 days.