• Animal cells and systems
• /
• 제16권1호
• /
• pp.41-49
• /
• 2012

Eighteen new polymorphic microsatellite markers were developed for the Korean mi-iuy croaker ($Miichthys$ $miiuy$, Perciformes, Sciaenidae), and allelic variability was compared between a wild population in Mokpo, Korea, and a hatchery population in Tongyeong, Korea. All loci were amplified readily and demonstrated allelic variability, with the number of alleles ranging from 5 to 37 in the wild population, and from 4 to 12 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, to be 0.74 and 0.78 in the hatchery population samples, and 0.79 and 0.86 in the wild samples. These results indicate lower genetic variability in the hatchery population compared with the wild population, and significant genetic differentiation between the wild population and the hatchery samples ($F_{ST}$=0.058, P<0.001). These microsatellite loci may be valuable for future population genetic studies, monitoring changes in the genetic variation within stocks in a commercial breeding program, conservation genetics, and molecular assisted selective breeding of the mi-iuy croaker in the future.

• 한국양식학회지
• /
• 제21권4호
• /
• pp.346-350
• /
• 2008

Haliotis속 전복류 내에서 종간의 유전적 차이 및 유연관계를 나타내는 유전표식으로 RAPD를 사용하여 그 유용성 정도를 파악하기 위하여 북반구 2종(H. discus hannai와 H. rufescens)과 남반구 2종(H. rubra와 H. midae)을 대상으로 RAPD 분석을 행하였다. 그 결과 RAPD는 Haliotis 종간의 구분은 명확히 구분할 수 있는 유용한 유전표식으로 평가되었으나, 유전적 유연관계에 있어서는 형태학적 및 지리학적 분포에 따른 유연관계와는 상이하게 나타났다.

• Journal of Ecology and Environment
• /
• 제43권4호
• /
• pp.454-461
• /
• 2019

Background: Ikonnikov's whiskered bat (Myotis ikonnikovi) is found throughout the Korean Peninsula, as well as in Kazakhstan, Russia, Mongolia, China, and Japan. It is small-sized and primarily inhabits old-growth forests. The decrease and fragmentation of habitats due to increased human activity may influence the genetic structure of bat populations. This study was designed to elucidate the population genetic structure of M. ikonnikovi using mitochondrial genes (cytochrome oxidase I and cytochrome b). Results: The results showed that M. ikonnikovi populations from Korea have high genetic diversity. Although genetic differentiation was not detected for the COI gene, strong genetic differentiation of the Cytb gene between Mt. Jeombong and Mt. Jiri populations was observed. Moreover, the results indicated that the gene flow of the maternal lineage may be limited. Conclusions: This study is the first to identify the genetic population structure of M. ikonnikovi. We suggest that conservation of local populations is important for sustaining the genetic diversity of the bat, and comprehensive studies on factors causing habitat fragmentation are required.

• 한국자원식물학회지
• /
• 제27권6호
• /
• pp.660-670
• /
• 2014

The Korean plum yew (Cephalotaxus koreana Nakai) is a shade-tolerant, coniferous shrub. The seeds have been used as a folk medicine in Korea, and an alkaloid extract (HTT) is known to have anticancer properties. We estimated the genetic diversity of 429 trees in 16 populations in South Korea using 194 polymorphic amplicons from seven combinations of AFLP primer-restriction enzymes. The average number of effective alleles and the percentage of polymorphic loci were 1.37 and 79.4%, respectively. Shannon's diversity index and the expected heterozygosity were 0.344 and 0.244, respectively. We divided 16 populations into four groups on the UPGMA dendrogram and the PCA biplot. The first two principal components explained 84% of the total genetic variation. Genetic differentiation between populations explained 14% of total genetic variation, and the remaining 86% came from difference between individuals within populations, as determined by an analysis of molecular variance (AMOVA). However, the genetic differentiation did not correlate with the geographic distance between populations from the Mantel test. The Bayesian statistics, which are comparable to Wright's $F_{ST}$ and Nei's $G_{ST}$, were ${\theta}^I=0.406$ and ${\theta}^{II}=0.172$, respectively. The population genetic diversity was slightly lower, and the strength of genetic differentiation was much weaker, than the average of those plants having similar life histories, as assessed using arbitrary marker systems. We discuss strategies for the genetic conservation of the plum yew in Korea.

• 생약학회지
• /
• 제25권4호통권99호
• /
• pp.395-398
• /
• 1994

A diterpene alkaloid in addition to hypaconitine and mesaconitine was isolated from the tubers of Aconitum napiforme (Ranunculaceae), which is an indigenous plant in Korea. Its structure was elucidated as 8-methoxy 14-benzoyl hypaconine by Physicochemical and spectroscopic data. This compound was isolated from this plant for the first time.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
• /
• pp.99.2-99.2
• /
• 2003

One of the major limitations in using adenoviral vector for gene therapy is inefficient infection of host cells. The presence of coxsackievirus and adenovirus receptor (CAR) and ${\alpha}$-integrin on cell surfaces is required for efficient adenovirus infection. In this study, we investigated the effect of trichostatin A, a histone deacetylase inhibitor, on transfection efficiency after transduction of adenovirus mediated p16$\^$INK4a/ gene transfer. In our previous study, p16$\^$INK4a/ tumor suppressor gene transfer in the non-small cell lung cancer cells (A549 cells) by transduction of recombinant adenovirus (Ad5CMV-p16) resulted in significant inhibition of cancer cell proliferation. (omitted)

• 대한약학회:학술대회논문집
• /
• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
• /
• pp.246.1-246.1
• /
• 2002

We studied the effects of ursodeoxycholic acid (UDCA) and its synthetic derivatives. HS-l030 and HS-1183. and chenodeoxycholic acid (CDCA) and its synthetic derivatives, HS-1199 and HS-1200. on the human colon adenocarcinoma cell line. HT -29 (p53 mutant type). The effects on cell viability and growth were assessed by MTT assay and cell growth study. While UDCA and CDCA exhibited no significant effect, their novel derivatives inhibited the proliferation of HT-29 cell line in a concentration- and time-dependent manners. (omitted)

• Applied Biological Chemistry
• /
• 제37권2호
• /
• pp.110-114
• /
• 1994

토양에서 분리된 방선균에서 생산되는 살충성 물질을 스크리닝하는 연구과정중 선발된 no. 46균주가 생산하는 살충성 물질을 분리하였다. 분리된 물질 4가지는 UV, $^1H-NMR$, $^{13}C-NMR$, C-H COSY 방법으로 구조 분석한 결과, Piericidin $C_1$, $C_2$, $C_3$ 그리고 $D_1$으로 각각 동정되었다.

• 한국국방경영분석학회지
• /
• 제35권3호
• /
• pp.47-59
• /
• 2009

A hybrid Genetic Algorithm is applied to military resource allocation problem. Since military uses many resources in order to maximize its ability, optimization technique has been widely used for analysing resource allocation problem. However, most of the military resource allocation problems are too complicate to solve through the traditional operations research solution tools. Recent innovation in computer technology from the academy makes it possible to apply heuristic approach such as Genetic Algorithm(GA), Simulated Annealing(SA) and Tabu Search(TS) to combinatorial problems which were not addressed by previous operations research tools. In this study, a hybrid Genetic Algorithm which reinforces GA by applying local search algorithm is introduced in order to address military optimization problem. The computational result of hybrid Genetic Algorithm on Missile Interceptor Allocation problem demonstrates its efficiency by comparing its result with that of a simple Genetic Algorithm.

• Archives of Pharmacal Research
• /
• 제17권2호
• /
• pp.100-103
• /
• 1994

The limonoid compound (28-deacetyl sendanin0 isolated from the fruit of Melia toosendan SIEB. et ZUCC. was evaluated on anticancer activity. According to a standard in vitro cytotoxicity assy, eight human cancer cell lines and SRB assay were introduced for present evaluation. As a positive standard, adriamycin was tested in parallel. The cell lines were originated from six different organs. In view of dose-response profiles to 28-deacetyl sendanin, the most sensitive cells were SF-539 and PC-3 which were derived from CNS and prostate, respecitively. In contrast, all the cell lines responded similarly to adriamycin to give rise to nearly indentical six cell lines were more sensitive to 28-deacetyl sendanin and two were more resistant. As a result, 28-deacetyl sendanin had more senstive and selective inhibitory effects on in vitro growth of human cancer cell lines in a comparison with adriamycin.