• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.207-215
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• 2004

The present study was carried out to evaluate the ISSR and RAPD markers for their efficiency as genetic marker systems to establish the relationships between 18 mulberry genotypes. A total of 36 from 56 (64%) RAPD primers and 12 from 48 (25%) ISSR primers produced reproducible amplification patterns. A high proportion of polymorphic bands ranging from 44 to 91% was observed respectively with RAPD and ISSR markers. The average Resolving Power (Rp) of ISSR primers was higher than RAPD primers. The ISSR primers, UBC 825, 868 and 873, and RAPD primers, UBC 712, 720 and 729, possessed the highest Rp values and could in each instance distinguish all the 18 genotypes. Similarity matrix values were estimated based on Jaccards coefficient, considering 109 polymorphic ISSR and 212 polymorphic RAPD bands and two dendrograms were constructed. The dendrograms obtained with ISSR and RAPD markers distinguished the eight exotic genotypes from the ten indigenous (Indian) genotypes. A significant correlation value (r=0.959; p=0.001) for the cophenetic matrix between the RAPD and ISSR matrices was observed. The results indicated that the ISSR and RAPD markers could assist in the differentiation of genotypes and permit the determination of genetic distances that might be exploited by mulberry breeders in improvement programs.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제23회 추계학술대회 논문집
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• pp.195-200
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• 2004

본 연구에서는 실험데이터와 유전자 알고리즘으로 압축기 성능선도를 생성하는 방법을 제안하였다. 다수의 실험을 통해 엔진의 성능 데이터를 획득하고 회전수에 따른 유량함수, 압력비, 효율의 함수관계를 3차 방정식으로 유도한 후 유전자 알고리즘을 이용하여 미계수를 구하여 압축기 성능선도를 생성하였다. 새롭게 생성한 압축기 성능선도를 이용하여 상용 성능해석 프로그램인 GASTURB로 정상상태 성능해석을 수행하여 검증데이터와 비교하였다.

• Molecules and Cells
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• 제42권2호
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• pp.104-112
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• 2019

Tracking the fate of individual cells and their progeny through lineage tracing has been widely used to investigate various biological processes including embryonic development, homeostatic tissue turnover, and stem cell function in regeneration and disease. Conventional lineage tracing involves the marking of cells either with dyes or nucleoside analogues or genetic marking with fluorescent and/or colorimetric protein reporters. Both are imaging-based approaches that have played a crucial role in the field of developmental biology as well as adult stem cell biology. However, imaging-based lineage tracing approaches are limited by their scalability and the lack of molecular information underlying fate transitions. Recently, computational biology approaches have been combined with diverse tracing methods to overcome these limitations and so provide high-order scalability and a wealth of molecular information. In this review, we will introduce such novel computational methods, starting from single-cell RNA sequencing-based lineage analysis to DNA barcoding or genetic scar analysis. These novel approaches are complementary to conventional imaging-based approaches and enable us to study the lineage relationships of numerous cell types during vertebrate, and in particular human, development and disease.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제4권2호
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• pp.63-68
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• 2023

The Kentish Plover (Charadrius alexandrinus ; family Charadriidae; genus Charadrius) is a small bird that moves from continent to continent depending on the season. On the Kentish Plovers, phylogenetic studies have been widely conducted to classify different species or subspecies and to determine the time of speciation. However, the perspectives on the interspecific or intraspecific relationships in the phylogenetic analysis of Kentish Plovers remain debatable. Here, we reviewed the differences between the Kentish and Snowy Plovers (C. nivosus ) in terms of their morphology, ecology, and genetic information. Particularly, their differences in genetic information can be well demonstrated; however, the intraspecies differences in the populations that live in different environments can relatively be poorly explained. We suggest that not only genetic features but also morphological, ecological, and behavioral traits are important when comparing the Kentish Plovers with other species, such as the Snowy Plovers, in phylogenetic studies. Furthermore, we suggest that phylogenetic studies on the subspecies of the Kentish and Snowy Plovers should be conducted for their better identification.

• Animal cells and systems
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• 제3권3호
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• pp.303-309
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• 1999

Inter- and intraspecific genetic relationships between Rana amurensis from Korea and Russia and other brown frogs were investigated by nucleotide sequence of a 504 base pair (bp) fragment of the mitochondrial cytochrome b gene. Nucleotide sequence similarities among Korean populations of R. amurensis ranged from 99.6% to 97.6% and 98.8% within Russian populations. The nucleotide sequence similarity between Korean and Russian R. amurensis ranged from 86.9% to 85.5%. Based on Kimura-2-parameter distance, the sequence divergence between R. amurensis from Korea and Russia was 16.18% and 18.04% among other related brown frogs. interspecific sequence divergences among R. amurensis and other related brown frogs diverged by 20.3%. Using an estimate of 2-4% mitochondrial DNA sequence divergence per million years, Korean and Russian R. amurensis diverged about 8 to 4 million years ago (Mya) and other brown frogs diverged about 9 to 5 Mya from ancestral frogs and distributed from North Asia to Sakhalin in a short time. In the neighbor-joining and UPGMA tree R. amurensis was clustered into two groups with Korean and Russian populations and the other brown frogs were grouped separately with diverged trichotomous clusters (R. dybowskii and R. pirica, R. okinavana and R. tsushimensis, and R. japonica and R. longicrus).

• 한국지반공학회논문집
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• 제20권5호
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• pp.37-48
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• 2004

본 연구에서는 사질토의 취성적 응력-변형률 관계와 전단시 체적팽창을 고려할 수 있는 구성모델에 대한 연구를 수행하였다. 제안된 모델은 일반등방경화규칙에 의거한 비등방 경화규칙을 적용하였으며, 미소변형에서 대변형에 이르는 전체변형률 영역의 거동을 모델할 수 있도록 적합한 경화함수를 이용하였다. 항복면의 형태는 응력공간에서 원통형으로 나타나는 단순한 형태로 실용적으로 적용하기 편리하도록 하였다. 또한 유동규칙을 단순화하여 소성 체적 변형률을 팽창률을 이용하여 정의하였다. 이로 인하여 사질토에서 나타나는 전단시 팽창을 모델하는 것이 가능하였다. 또한 가상적인 첨두응력비를 정의하여 취성적 응력-변형률 관계를 모델하는 것이 가능하였다. 이 때 제안된 모델의 계수를 체계적으로 결정하기 위하여 실수형 유전자 알고리즘이 적용된 최적화 기법이 적용되었다. 이를 통하여 구성 모델에 필요한 계수를 결정할 수 있었다. 제안된 모델을 검증하기 위하여 풍화토시료에 대한 $K_0$ 압밀 삼축시험을 수행하였다. 이러한 시험결과를 제안된 모델과 비교한 결과 $K_0$ 압밀 시험에서 나타나는 취성적 응력-변형률 관계 및 체적의 팽창과 같은 실제 유효응력 거동을 합리적으로 모델하는 것이 가능하였다.

• 한국버섯학회지
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• 제8권1호
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• pp.27-32
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• 2010

본 시험은 국내외에서 수집한 꽃송이버섯균 22균주에 대하여 분자생물학적 유연관계를 분석하고자 하였다. 수집균주의 ribosomal DNA의 ITS 영역에 대한 cleaved amplified polymorphic sequence (CAPS) 분석 결과, KACC50866은 다른 균주들과 20%이하의 유연관계를 나타내었으며 나머지 균주들은 90% 이상의 유연관계를 보이면서 4그룹으로 구분되었다. 따라서 이들의 세분화된 분자생물학적 구분을 위하여 rDNA ITS 영역의 염기서열분석을 하여 구분하여 본 결과 KACC50866 균주는 다른 꽃송이버섯균과 유연관계가 매우 낮은 것으로 나타났다. 그리고 나머지 21개 균주는 같은 그룹으로 구분되어 있어 같은 종으로 생각할 수 있으나, 이들을 좀더 세분하기 위해서는 미토콘드리아의 유전자 서열 분석 등이 병행되어야 할 것으로 판단된다.

• 식물분류학회지
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• 제36권4호
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• pp.293-308
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• 2006

종간 유연관계와 다배체종의 기원을 규명하기 위해 한국산 꿩의비름속 식물 6종 20집단에 대해 형태형질과 동위효소 자료를 이용하여 유전적, 형태적 연구를 수행하였다. 형태 형질 분석결과에 의하면 삼배체인 새끼꿩의비름은 세잎꿩의비름과 매우 가까운 유연관계를 보여 주고 있고, 동위효소 분석에서도 높은 빈도로 나타나는 대립유전자에서도 두 종이 매우 유사한 양상을 보여 주었다. $PGI-1^b$의 경우 두 종에서만 독특하게 고정되어 있어 세잎꿩의비름이 새끼꿩의비름의 이배체 조상일 가능성을 시사하였다. 하지만 $ALD-1^c$ 대립인자의 경우, 새끼꿩의비름에서만 독특하게 나타나며, 꿩의비름과 큰꿩의비름에서 공통으로 나타나는 대립인자들이 새끼꿩의비름에서는 나타나지 않아 위 두 종이 새끼꿩의비름의 또 다른 사배체 조상일 가능은 희박하다고 하겠다. 우리나라 특산종인 섬꿩의비름은 형태적으로나 유전적으로 꿩의비름과 매우 유사한 양상을 보여주고 있어 꿩의비름으로부터 직접 또는 공통의 조상으로부터 나왔을 가능성이 크다. 또한, 둥근잎꿩의비름은 꿩의비름, 섬꿩의비름과 가까운 유연관계를 보여주고 있다.

• Animal cells and systems
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• 제13권3호
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• pp.323-329
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• 2009

We analyzed partial sequences of cytochrome b (cyt-b), a mitochondrial DNA (mtDNA) gene, to determine the genetic relationships between six horsehead fish species: Branchiostegus japonicus, Branchiostegus albus, Branchiostegus auratus, Branchiostegus argentatus, Branchiostegus wardi, and an unidentified Branchiostegus species. The specimens were collected in Korea, China, Japan, and Vietnam. We compared their molecular phylogenetic relationships inferred from mtDNA cyt-b sequences with an osteological analysis. The unidentified species, B. sp., was similar to B. albus in terms of the lack of triangular silver-white dot at the posterior region of eyes (vs. large one present in B. japonicus), but was also similar to B. japonicus in terms of the presence of a straight-shaped first hemal spine (vs. a curve-shaped hemal spine in B. albus). Analysis of the mtDNA cyt-b sequences indicated that the smallest estimated sequence divergence was between the B. japonicus and B. sp. (0.70-0.94%), whereas the largest difference was between B. auratus and B. argentatus (23.06-23.36%). Both the maximum parsimony and maximum likelihood trees showed that the B. sp. was closely clustered with B. japonicus, and that B. auratus was most distant from the other species. When comparing the osteological characters, UPGMA tree showed that the B. japonicus and B. sp. were the most closely clustered species, and B. auratus was the most distantly clustered fish relative to the other species. The shape of the nasal, otolith and first hemal spine was informative for distinguishing B. auratus from the other species. These osteological differences were consistent with the differences in mtDNA.

• The Plant Pathology Journal
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• 제35권3호
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• pp.189-199
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• 2019

In this research, the relationships among the 31 microsatellite markers with charcoal rot disease resistance related indices in 130 different soybean cultivars and lines were evaluated using association analysis based on the general linear model (GLM) and the mixed linear model (MLM) by the Structure and Tassel software. The results of microsatellite markers showed that the genetic structure of the studied population has three subpopulations (K=3) which the results of bar plat also confirmed it. In association analysis based on GLM and MLM models, 31 and 35 loci showed significant relationships with the evaluated traits, respectively, and confirmed considerable variation of the studied traits. The identified markers related to some of the studied traits were the same which can probably be due to pleiotropic effects or tight linkage among the genomic regions controlling these traits. Some of these relationships were including, the relationship between Sat_252 marker with amount of charcoal rot disease, Satt359, Satt190 and Sat_169 markers with number of microsclerota in stem, amount of charcoal rot disease and severity of charcoal rot disease, Sat_416 marker with number of microsclerota in stem and amount of charcoal rot disease and the Satt460 marker with number of microsclerota in stem and severity of charcoal rot disease. The results of this research and the linked microsatellite markers with the charcoal rot disease-related characteristics can be used to identify the suitable parents and to improve the soybean population in future breeding programs.