• Title/Summary/Keyword: Genetic Relationships

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Genetic Diversity and Molecular Phylogenetic Relationships of the Genus Sarcocheilichthys Fish in Korea (한국산 중고기속(Sarcocheilichthys) 어류의 유전적 다양성과 분자계통학적 유연관계)

  • Ji-Wang Jang;Jae-Goo Kim;Jae-Geun Ko;Bong-Han Yun;Yang-Seop Bae
    • Korean Journal of Ichthyology
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    • v.36 no.2
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    • pp.139-155
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    • 2024
  • Using the cytb gene region of the mitochondrial DNA of eight populations of Sarcocheilichthys nigripinnis morii and five populations of S. variegatus wakiyae, which belong to the genus Sarcocheilichthys from Korea, the genetic diversity and molecular phylogenetic relationships of each population were examined. As a result of the analysis, it was confirmed that the S. variegatus wakiyae population had higher genetic diversity than the S. nigripinnis morii population. In the phylogenetic tree of genus Sarcocheilichthys fish in Korea based on the cytb gene, the Yeongsan River (YSR) population of S. variegatus wakiyae forms a clade with the Tamjin River (TJR), Yeongsan River (YSR), and Seomjin River (SJR) population of S. nigripinnis morii, and genetic relationships that do not align with the current classification system were observed. Meanwhile, on the nuclear DNA phylogenetic tree, S. variegatus wakiyae and S. nigripinnis morii could be clearly distinguished, showing mitonuclear inconsistency where mitochondrial and nuclear DNA conflicted on the phylogenetic tree. The Seomjin River (SJR) population of S. nigripinnis morii was translocated to the Dongjin River (DJR) population, haplotype from which crossbreeding was presumed to have occurred was confirmed. Among the rivers flowing into the East Sea, the S. nigripinnis morii population is known to have been introduced and inhabit only the Hyeongsan River (HSR), and it is presumed to be a population formed by translocation from the Han River (HR) population, with a haplotype representing a unique genetic group also confirmed. The Han River (HR), Geum River (GR), and Mangyeong River (MGR) populations of S. nigripinnis morii formed a genetically identical population with S. czerskii and S. soldatovi distributed north of the Yalu River, and accordingly, a taxonomic reexamination was required through morphological and molecular phylogenetic studies by securing various specimens.

Genetic Diversity and Relationships of Korean Chicken Breeds Based on 30 Microsatellite Markers

  • Suh, Sangwon;Sharma, Aditi;Lee, Seunghwan;Cho, Chang-Yeon;Kim, Jae-Hwan;Choi, Seong-Bok;Kim, Hyun;Seong, Hwan-Hoo;Yeon, Seong-Hum;Kim, Dong-Hun;Ko, Yeoung-Gyu
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.10
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    • pp.1399-1405
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    • 2014
  • The effective management of endangered animal genetic resources is one of the most important concerns of modern breeding. Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. This study aimed to analyze the genetic diversity and population structure of six Korean native chicken breeds (n = 300), which were compared with three imported breeds in Korea (n = 150). For the analysis of genetic diversity, 30 microsatellite markers from FAO/ISAG recommended diversity panel or previously reported microsatellite markers were used. The number of alleles ranged from 2 to 15 per locus, with a mean of 8.13. The average observed heterozygosity within native breeds varied between 0.46 and 0.59. The overall heterozygote deficiency ($F_{IT}$) in native chicken was $0.234{\pm}0.025$. Over 30.7% of $F_{IT}$ was contributed by within-population deficiency ($F_{IS}$). Bayesian clustering analysis, using the STRUCTURE software suggested 9 clusters. This study may provide the background for future studies to identify the genetic uniqueness of the Korean native chicken breeds.

Interspecific Relationships of the Genus Leucophenga(Drosophiliae) by Electrophoretic Analysis of Protein (단백질 전기영동 분석에 의한 Leucophenga 속 (Drosophilidae) 초파리의 종간 유연관계)

  • 이택준;오준영
    • Animal Systematics, Evolution and Diversity
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    • v.7 no.1
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    • pp.65-72
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    • 1991
  • The interspecific relationships of the genus Leucophenga, L.concilia, L.maculata, L.orientalis , and L.quinquenmaculipennis were investigated by soluble proteins analysis. the electrophoretic banding patterns of the four species analyzed by SDS-PAGE were scanned by densitormeter. The soluble protein patters of L.maculata and L.concilia by SDS-PAGE appeared similarly and on the other hand, those of L.quinquemaculipennis had a different forms from three other species. The genetic distance among the four species of TDE analysis was calculated by formula of the Aquadro and Avis(1981) . The genetic distance between L.maculata and L.concilia was 0.393 , the lowest of all and between L.maculata and L.quinquemaculipennis was 0.496, the highest of all. The dendrogram was established using UPGMA method based on genetic distance obtained by TDE analysis. Clustered patterns as follows ; first, L.maculata and L.concilia were clustered, and L.orientalis and L. quinquemaculipennis followed the firstone in order. In consequence, it seems that the interspecific relationship of L.quinquemaculipennis was farther from the other three species, while that of L.maculata and L.concilia was closer than that of L.orientalis.

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Genetic Diversity Analysis of South and East Asian Duck Populations Using Highly Polymorphic Microsatellite Markers

  • Seo, Dongwon;Bhuiyan, Md. Shamsul Alam;Sultana, Hasina;Heo, Jung Min;Lee, Jun Heon
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.4
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    • pp.471-478
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    • 2016
  • Native duck populations have lower productivity, and have not been developed as much as commercials duck breeds. However, native ducks have more importance in terms of genetic diversity and potentially valuable economic traits. For this reason, population discriminable genetic markers are needed for conservation and development of native ducks. In this study, 24 highly polymorphic microsatellite (MS) markers were investigated using commercial ducks and native East and South Asian ducks. The average polymorphic information content (PIC) value for all MS markers was 0.584, indicating high discrimination power. All populations were discriminated using 14 highly polymorphic MS markers by genetic distance and phylogenetic analysis. The results indicated that there were close genetic relationships among populations. In the structure analysis, East Asian ducks shared more haplotypes with commercial ducks than South Asian ducks, and they had more independent haplotypes than others did. These results will provide useful information for genetic diversity studies in ducks and for the development of duck traceability systems in the market.

Comparison of Genetic Diversity among Amaranth Accessions from South and Southeast Asia using SSR Markers (SSR 마커를 이용한 남아시아와 동남아시아 아마란스 자원의 유전적 다양성 비교)

  • Wang, Xiao Qiang;Park, Yong Jin
    • Korean Journal of Medicinal Crop Science
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    • v.21 no.3
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    • pp.220-228
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    • 2013
  • This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.

Assessment of Genetic Diversity and Population Structure on Kenyan Sunflower (Helianthus annus L.) Breeding Lines by SSR Markers

  • Mwangi, Esther W.;Marzougui, Salem;Sung, Jung Suk;Bwalya, Ernest C.;Choi, Yu-Mi;Lee, Myung-Chul
    • Korean Journal of Plant Resources
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    • v.32 no.3
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    • pp.244-253
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    • 2019
  • In crop breeding program, information about genetic dissimilarity on breeding resources is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations and inbred breeding. This study aimed to evaluate the genetic variation in Kenyan sunflower breeding lines based on simple sequence repeat (SSR). A total of 83 alleles were detected at 32 SSR loci. The allele number per locus ranged from 2 to 7 with an average of 2.7 alleles per locus detected from the 24 sunflower accessions and the average value of polymorphic information contents (PIC) were 0.384. A cluster analysis based on the genetic similarity coefficients was conducted and the 24 sunflower breeding resources were classified into three groups. The principal coordinates (PCoA) revealed 34% and 13.38% respectively, and 47.38% of total variation. It was found that the genetic diversity within the Kenyan sunflower breeding resources was narrower than that in other sunflower germplasm resources, suggesting the importance and feasibility of introducing elite genotypes from different origins for selection of breeding lines with broader genetic base in Kenyan sunflower breeding program.

Genetic diversity and population structure of Korean eelgrass, Zostera mirina

  • Huh, Man-Kyu;Cui, Jin-Zi;Lee, Hak-Young;Lee, Kun-Seop;Lee, Bok-Kyu
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.277-278
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    • 2003
  • The Korean populations of Z. marina are typically distributed in patches. Although molecular and biochemical approaches are now increasingly being applied to address the taxonomic and phylogenetic relationships within the animals, plants, and other algal species in Korea (Huh 1998; Hwang et at. 1998; Huh 2001), no population genetic studies of this species have been conducted. (omitted)

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