• Title/Summary/Keyword: Genetic Divergence

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Intraspecific Molecular Phylogeny, Genetic Variation and Phylogeography of Reticulitermes speratus (Isoptera:Rhinotermitidae)

  • Park, Yung Chul;Kitade, Osamu;Schwarz, Michael;Kim, Joo Pil;Kim, Won
    • Molecules and Cells
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    • v.21 no.1
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    • pp.89-103
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    • 2006
  • Population structure was investigated in Reticulitermes speratus populations in the Korean Peninsula and the Japanese Archipelago. All trees derived from analyses of the combined sequence dataset of two mitochondrial genes, COII and COIII, showed that R. speratus populations cluster into two major clades comprising the Korean/southern Japanese populations and the northern Japanese populations. Analysis of population genetic structure showed strong genetic partitioning between populations of the two clades. To understand historical migration routes and current distributions, the phylogeographic history of R. speratus was inferred from intra-/interspecific phylogeny and divergence times estimated between the clades of the phylogenetic tree. The estimated migration route and divergence time of ancestral R. speratus are congruent with recent paleogeographic hypotheses involving land-bridge connections between the Asian continent and the Japanese Archipelago. We suggest that ancestral R. speratus separated into northern and southern Japanese populations after its migration into the Japanese main islands from East China during the early Pleistocene via the East China Sea basin, which may have been exposed during that period. The Korean populations seem to have diverged recently from southern Japanese populations; this may explain the current distribution of R. speratus in the Japanese Arachipelago, and account for why it is restricted to northern areas of the Tokara Strait.

Analysis of genetic divergence according to each mitochondrial DNA region of Haliotis discus hannai (북방전복 (Haliotis discus hannai) 의 mitochondrial DNA 영역별 유전적 변이성 분석)

  • Park, Choul-Ji;Nam, Won Sick;Lee, Jeong-Ho;Noh, Jae Koo;Kim, Hyun Chul;Park, Jong Won;Hwang, In Jun;Kim, Sung Yeon
    • The Korean Journal of Malacology
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    • v.29 no.4
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    • pp.335-341
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    • 2013
  • The seven mitochondrial DNA regions (ND2, ND5, ND4, ND4L, ND6, ND1 and 12SrRNA) of Haliotis discus hannai were examined to estimate the availability as a genetic marker for the study of population genetic. The region with the highest genetic variation was ND4 (Haplotype diversity = 1.0000, Nucleotide diversity = 0.0108). On the other hand, ND2 and ND1 regions have significantly appeared genetic divergence between clusters (divergence of 90% and 87%). Also, pairwise $F_{ST}$ between clusters within ND2 and ND1 regions showed high values; 0.4061 (P = 0.0000), 0.4805 (P = 0.0000) respectively. Therefore we can infer that it is the most efficient and accurate way to analyze the region of ND4 with the highest variation in addition to the regions of ND2 and ND1, which formed clusters with high bootstrap value, for study of population genetic structure in this species.

Haplotype Diversity and Gene Flow of the Diamondback Moth, Plutella xylostella(L.) (Lepidoptera: Yponomeutidae), in Korea (배추좀나방(나비목: 집나방과)의 haplotype 다양성과 유전자 이동률)

  • 김익수;배진식;최광호;진병래;이경로;손흥대
    • Korean journal of applied entomology
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    • v.39 no.1
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    • pp.43-52
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    • 2000
  • A portion of mitochondria1 COI gene (438 bp) was sequenced from the sampls of Plutella xylostella from four localities in Korea to investigate the population genetic structure and characteristics by measuring the magnitude of genetic diversity and the degree of gene flow among populations. Thirteen haplotypes ranging in nucleotide divergence 0.3% to 1.4%, were obtained from 21 individuals. The nucleotide divergence was similar to the other related studies, but haplotype diversity was substantially higher (mean h = 0.81). The genetic distance among geographically remote Cheju Island population and the two Kimhae populations, distant 1 lkm to each other, was not statistically significant (p<0.05). Instead, a substantial or high female gene flow was detected (Nm = 2-30). One Hawaiian haplotype of the diamondback moth obtained through GenBank search also was genetically similar to the ones obtained from this study. Collectively, the genetic population structure of the diamondback moth in Korea can be characterized into two aspects. First, the diamondback moths in Korea possesses overall moderate genetic divergence based on a high number of haplotypes. Second, a high haplotype diversity within each population due to the long distance dispersal with a substantial dispersal power and the resultant genetic similarity among geographic populations is characteristic.

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Mitochondrial DNA Sequence Variation of the Mason Bee, Osmia cornifrons (Hymenoptera: Apidae)

  • Kim, Hwa-Young;Lee, Kyeong-Yong;Lee, Sang-Beom;Kim, Se-Ryeon;Hong, Mee-Yeon;Kim, Dong-Young;Kim, Ik-Soo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.16 no.2
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    • pp.75-86
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    • 2008
  • In order to understand geographic genetic variation and relationship among populations of the mason bee (Osmia cornifrons Radoszkowsky), which is used as pollinator for apple tree, we sequenced a portion of mitochondrial (mt) COI gene, which corresponds to "DNA Barcode" region (658 bp) from 81 O. cornifrons individuals collected over eight localities in Korea. The sequence data revealed overall moderate to low genetic diversity within species, with a maximum sequence divergence of 0.76%. Geographically, two haplotypes (BAROC01 and BAROC02) were widespread with a frequency of 82.7%, whereas several haplotypes were found in a locality as a single individual, suggesting that haplotype distribution can be summarized as coexistence of a few widespread haplotypes and several regionally restricted haplotypes. Overall, high rate of per generation female migration (Nm=$1.1{\sim}$infinite) and low level of geographic subdivision ($F_{ST}=0{\sim}0.315$) among localities were characteristic. Although two populations (p < 0.026) were genetically subdivided from the remaining localities, no clear polarity was observed. Taken together, the nature of genetic divergence of the mason bee populations is characterized as one that possessing moderate to low genetic diversity, high gene flow, and wide spread haplotypes with ahigh frequency, concordant with the capability of dispersal in connection with the lack of historical biogeographic barriers.

Relationship Between Heterosis and Genetic Divergence in Tongil-type Rice(Oryza sativa L.) (벼에서 RAPDs 분석에 의한 양친의 유전적 유사성과 잡종강세의 상관)

  • Ahn, Sang-Nag;Kim, Yun-Sun
    • Korean Journal of Agricultural Science
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    • v.28 no.1
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    • pp.1-7
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    • 2001
  • In this study we evaluated genetic divergence among six Tongil-type rice varieties and assessed the relationship between genetic distance and hybrid performance in all possible non-reciprocal crosses between them. The 15 F1 hybrids along with the six parents were evaluated for eight traits of agronomic importance including yield in a replicated field trial. The six parents were examined for DNA polymorphism using 120 random decamer oligonucleotide primers. A total of 168 polymorphic variants were generated and based on the polymorphism data, genetic distances (GDs) ranged from 0.157 to 0.383. Heterosis was observed in hybrids for most of the traits, and yield exhibited the highest heterosis among the eight traits examined. The correlation values of GDs with F1 performance were not significant. Also the correlations of GDs with midparent and better-parent heterosis were not significant except for spikelets per panicle. Our results have indicated that GDs based on the RAPDs markers may not be useful for predicting heterotic combinations in Tongil-type rice and are supportive of the idea that the level of correlations between hybrid performance and genetic divergence is dependent on the germlplasm employed.

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Genetic Variation and Population Structure of Crepidiastrum lanceolatum (Compositae)

  • Huh, Man-Kyu;Choi, Joo-Soo
    • Journal of Life Science
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    • v.12 no.1
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    • pp.9-13
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    • 2002
  • Crepidiastrum lanceolatum ($H_{OUTT}$) $N_{AKAI}$ (Compositae) is distributed in East Asia including Korea. Genetic diversity and population structure of six C. lanceolatum populations in Korea and two populations in Japan were determined using genetic variation at 19 allozyme loci. The percent of polymorphic loci within the enzymes was 42.1%. Genetic diversity at the species level and at the population level was low (Hes : 0.077; Hep : 0.068, respectively), where-as the extent of the population divergence was relatively low ($G_{ST}$ : 0.093). One of the most striking features of this study was the more significant difference within populations than among populations. An indirect estimate of the number of migrants per generation (Nm : 2.44) indicated that gene flow was moderate among eight populations of the species. In addition, analysis of fixation indices revealed a slight heterozygosity deficiency in some populations and at some loci. Narrow geographic ranges, short-lived perennial herbaceous, and small population sizes are mainly associated with the low level of genetic variation.n.

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Genetic Characterization of Two Types of Sea Bass, Lateolabrax japonicus in Korea by Isozyme Analysis (Isozyme 분석에 의한 한국산 농어, Lateolabrax japonicus 2형간의 유전학적 특징)

  • Park Jung-Youn;Kim Kyung-Kil;Kim Yoon
    • Journal of Aquaculture
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    • v.9 no.4
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    • pp.437-444
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    • 1996
  • Genetic characterization and identification of two types of sea bass Lateolabrax japonicus were performed by examining electrophoretic patterns of isozymes. Twenty five loci coding for thirteen enzymes were detected in two types. Among the twenty five loci, one completely divergent loci (Pt-1) was observed between two types. Nei's genetic distance between two types was 0.12036. The estimated divergence time of these two types may have about $6.2{\times}10^5$ years ago. On the other hands, the expected average heterozygosity was 0.084 in not spotted type on the body surface and 0.067 in spotted type on the body surface. These results mean that the existance of two types of sea bass was established in present study which may have had genetic divergence.

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Mitochondrial DNA Sequence Variations and Genetic Relationships among Korean Thais Species (Muricidae: Gastropoda)

  • Lee, Sang-Hwa;Kim, Tae-Ho;Lee, Jun-Hee;Lee, Jong-Rak;Park, Joong-Ki
    • Animal Systematics, Evolution and Diversity
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    • v.27 no.1
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    • pp.1-17
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    • 2011
  • Thais Roding, 1798, commonly known as rock-shell, is among the most frequently found gastropod genera worldwide on intertidal rocky shores including those of Japan, China, Taiwan and Korea. This group contains important species in many marine environmental studies but species-level taxonomy of the group is quite complicated due to the morphological variations in shell characters. This study examined the genetic variations and relationships among three Korean Thais species based on the partial nucleotide sequences of mitochondrial cox1 gene fragments. Phylogenetic trees from different analytic methods (maximum parsimony, neighbor-joining, and maximum likelihood) showed that T. bronni and T. luteostoma are closely related, indicating the most recent common ancestry. The low sequence divergence found between T. luteostoma and T. bronni, ranging from 1.53% to 3.19%, also corroborates this idea. Further molecular survey using different molecular marker is required to fully understand a detailed picture of the origin for their low level of interspecific sequence divergence. Sequence comparisons among conspecific individuals revealed extensive sequence variations within the three species with maximum values of 2.43% in T. clavigera and 1.37% in both T. bronni and T. luteostoma. In addition, there is an unexpectedly high level of mitochondrial genotypic diversity within each of the three Korean Thais species. The high genetic diversity revealed in Korean Thais species is likely to reflect genetic diversity introduced from potential source populations with diverse geographic origins, such as Taiwan, Hong Kong, and a variety of different coastal regions in South China and Japan. Additional sequence analysis with comprehensive taxon sampling from unstudied potential source populations will be also needed to address the origin and key factors for the high level of genetic diversity discovered within the three Korean Thais species studied.

The Fuzzy Modeling by Virus-messy Genetic Algorithm (바이러스-메시 유전 알고리즘에 의한 퍼지 모델링)

  • 최종일;이연우;주영훈;박진배
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2000.11a
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    • pp.157-160
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    • 2000
  • This paper deals with the fuzzy modeling for the complex and uncertain system in which conventional and mathematical models may fail to give satisfactory results. mGA(messy Genetic Algorithm) has more effective and adaptive structure than sGA with respect to using changeable-length string and VEGA(Virus Evolution Genetic) Algorithm) can search the global and local optimal solution simultaneously with reverse transcription operator and transduction operator. Therefore in this paper, the optimal fuzzy model is obtained using Virus-messy Genetic Algorithm(Virus-mGA). In this method local information is exchanged in population so that population may sustain genetic divergence. To prove the surperioty of the proposed approach, we provide the numerical example.

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On Sweeping Operators for Reducing Premature Convergence of Genetic Algorithms (유전 알고리즘의 조기수렴 저감을 위한 연산자 소인방법 연구)

  • Lee, Hong-Kyu
    • Journal of Institute of Control, Robotics and Systems
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    • v.17 no.12
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    • pp.1210-1218
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    • 2011
  • GA (Genetic Algorithms) are efficient for searching for global optima but may have some problems such as premature convergence, convergence to local extremum and divergence. These phenomena are related to the evolutionary operators. As population diversity converges to low value, the search ability of a GA decreases and premature convergence or converging to local extremum may occur but population diversity converges to high value, then genetic algorithm may diverge. To guarantee that genetic algorithms converge to the global optima, the genetic operators should be chosen properly. In this paper, we analyze the effects of the selection operator, crossover operator, and mutation operator on convergence properties, and propose the sweeping method of mutation probability and elitist propagation rate to maintain the diversity of the GA's population for getting out of the premature convergence. Results of simulation studies verify the feasibility of using these sweeping operators to avoid premature convergence and convergence to local extrema.