• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.83-83
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• 2017

The National Agrobiodiversity Center (NAS, RDA, Republic of Korea) has continually collected new valuable genetic resources. In this study, we regenerated conserved kidney bean (Phaseolus vulgaris L.) germplasm which couldn't be available because of seed quantity and quality, and we also surveyed their morphological characters for the sustainable utilization. A total of 431 kidney bean accessions were regenerated and 18 morphological traits were surveyed according to the characterization guideline of RDA Genebank. Among the surveyed traits, flowering time ranged from May 23 to September 4 and 73.8% of tested accessions were mainly flowering in June. The maturity time ranged from July 1 to October 15 and main flowering time was July (91.4%). For plant type, 270 accs (62.6%) were climbing type followed by medium type of 86 accs (20.0%) and dwarf type of 65 accs (15.1%). The seed coat colors were various; yellow (34.6%), white (22.3%), brown (17.9%), red (10.7%), black (5.8%), violet (11%), pink (1.4%), navy (0.9%). Principal component analysis indicated that five principal components (PCs) with Eigen values >1 accounted for more than 65.8% variability. The first PC was more related to growth habits such as growth type, flowering time, and plant type. The second and third PCs showed higher values of the pigment characters such as seed coat color, flower color, and pod color. In fourth and fifty PCs, there were the higher positive values of the pod shapes. Our results provided insight into the characteristics kidney beans, thus the utilization basis of kidney beans might be elevated for bio-industry.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.75-84
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• 2015

Sesame (Sesamum indicum L.) is one of the most important oilseed crops with high oil contents and rich nutrient value. The development of a core collection could facilitate easier access to sesame genetic resources for their use in crop improvement programs and simplify the genebank management. The present study was initiated to the development and evaluation of a core collection of sesame based on 5 qualitative and 10 quantitative trait descriptors on 2,751 sesame accessions. The accessions were different countries of origin. About 10.1 percent of accessions were selected by using the power core program to constitute a core collection consisting of 278 accessions. Mean comparisons using t-test, Nei's diversity index of 10 morphological descriptors and correlation coefficients among traits indicated that the existing genetic variation for these traits in the entire collection has been preserved in the core collection. The results from this study will provide effective information for future germplasm conservation and improvement programs in sesame.

• Korean Journal of Veterinary Research
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• v.40 no.2
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• pp.301-310
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• 2000

The antimicrobial susceptibility, genotypes of enterotoxins(LT, STa) and pili(K88, 987P), and plasmid profiles were investigated with 102 Escherichia coli isolated from piglets showing diarrhea in Korea. Almost of them were susceptible to ceftiofur(99%), cefquinone(97.1%). However they showed resistance to bacitracin(100%), streptomycin(98%), vancomycin(97%), trimethoprim/sulfamethoxazole(87.2%), tetracycline(84.3%) in antimicrobial susceptibility test. Moreover, all of the isolates demonstrated resistance to more than 2, and 78% of them were resistant to more than 8 of total 17 drugs. Multiplex PCRs for genotyping of enterotoxins(LT, STa) and pili(K88, 987P) were established with primers designed based on sequences from Genebank. Seventeen strains(16.6%) of the isolates had STa gene, 11 strains(10.8%) of them had both STa and LT genes, and 18 strains(17.8%) had K88 gene. But none of the isolates harbored a gene exclusively encoding LT. The gene encoding 987P pili was not found in all isolates. Fifty-four strains of 102 isolates(52.9%) had plasmid with various sizes ranging from 125kb to 1.1kb. Numbers of plasmid per isolates were also various, from 1 to 9. Distinctive relationship between plasmid profiles and genotypes of enterotoxins and pili in the isolates was not found. These results might provide the basic knowledge to establish strategies for the treatment and prevention of colibacillosis in piglets.

• Korean Journal of Plant Resources
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• v.28 no.6
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• pp.697-703
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• 2015

Twenty apple germplasm accessions from the Korean Genebank were successfully cryopreserved using two-step freezing to back up genetic resources maintained by field collections. This study examined the morphological and genetic stability of cryopreserved dormant apple buds that were stored in liquid nitrogen, and then rewarmed and regrown. Whole plants were regenerated directly from dormant buds through budding without an intermediary callus phase. The cryopreserved buds produced high levels of shoot formation (76.2-100%), similar to those of noncryopreserved buds (91.3-100%), with no observed differences between cryopreserved and noncryopreserved materials. Three of the twenty cryopreserved apple germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including shoot length, leaf shape, leaf width/length ratio, and root length were observed between controls (fresh control and noncryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) markers. The ISSR markers produced 253 bands using four primers, ISSR 810, SSR 835, ISSR 864, and ISSR 899. These markers showed monomorphic banding patterns and revealed no polymorphism between the mother plant and regenerants before and after cryopreservation, suggesting that cryopreservation using two-step freezing does not affect the genetic stability of apple germplasm. These results show that two-step freezing cryopreservation is a practical method for long-term storage of apple germplasms.

• Toxicological Research
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• v.25 no.1
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• pp.35-40
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• 2009

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and related halogenated aromatic hydrocarbons elicit a diverse spectrum of biochemical and toxic responses in laboratory animals and mammalian cells in culture. Toxicity and carcinogenicity of TCDD is well established but the molecular mechanism is still poorly understood. Here, we found the noble responsive genes to TCDD using the differential display analysis. Treatment of HepG2 cells with TCDD showed a significantly different mRNA expression pattern from the untreated cells in differential display analysis. The differentially displayed bands were isolated and used as probes in dot blot and Northern blot analyses. Of thirty-five isolated differentially displayed bands, only two bands were confirmed as positive in dot blot and Northern blot analyses. The nucleotides sequences of these clones were analyzed and the search of Genebank database revealed that one clone is highly homologous with RanBP2 (Ras-related nuclear protein binding protein2; 92%) and the other is an unknown gene. RanBP2 is a nucleoporin with SUMO E3 ligase activity that functions in both nucleocytoplasmic transport and mitosis and its role as a novel tumor suppressor has been recently proposed. Thus, these results may suggest the clue elucidating the toxic mechanism of TCDD through RanBP2.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.63-63
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• 2019

Tea plant (Camellia sinensis (L.) O. Kuntze) has been cultivated widely in many developing Asian, African, and South American countries, where it is the most widely consumed beverage in the world next to water. It has critical importance to understand the genetic diversity and population sturcutre for effective collection, conservation, and utilization of tea germplasm. In this study, 410 tea accessions collected from South Korea were analyzed using 21 SSR markers. Among 410 tea accessions, 85.4% (310 accessions) accessions were collected from Jeollanam-do. A total of 286 alleles were observed, and the genetic diversity and evenness were estimated to be averagely 0.79 and 0.61, respectively, across all the tested samples. Using discriminant analysis of principal components, the four clusters were detected in 410 tea accessions. Among them, cluster 1 showed higher frequency of rare alleles (less than 1%) than other clusters. Using calculation of the index of association and rbaD value, each cluster showed a clonal mode of reproduction. The result of AMOVA showed that most of the variation observed was within populations (99%) rather than among populations (1%). Our results might contribute to provide data about genetic diversity for the conservation of tea germplasm and for future breeding programs.

• Journal of Mushroom
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• v.12 no.1
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• pp.41-51
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• 2014

Cauliflower mushroom widely known high concent of ${\beta}$-glucan for farm cultivation invigoration verified characteristics of mycelia growth, genetic diversity, resistance to Trichoderma by replacement culture with Trichoderma and growth characteristics of new variety crossbleeding strain. The result of replacement culture with Trichoderma for verification resistance about Trichoderma, 6951 (T. viride) strain did not show special change after formation of confrontation line and 6952 (T. spp.) strain was showed more formation of spore after formation of confrontation line. But 6426 (T. harzianum) strain found to encroach part of growth area of cauliflower mushroom mycelia. Among 10 kinds cauliflower mushroom strain, JF02-06 strain collected by Gurye, found did not spore of Trichoderma and thought to be resistant to Trichoderma. The result of crossbleeding after selected that mother strain good growth and formation of fruit body, verified good mycelia growth at JF02-47, 49 and 50 strain in Korean pine of wood-chip media. The result of gene sequence about ITS1, 5.8S and ITS4 for analysis of genetic diversity at crossbleeding strain, found high significance to other cauliflower mushroom in registered Genebank. The result of growth characteristic of spore and mycelia of cauliflower mushroom by observation microscope, size of spore showed water drop shape to major axis $6{\mu}m$ and minor axis $5{\mu}m$ and clamp showed 3 types in mycelia. The wide of mycelia was $3{\mu}m$. The characteristic of mycelia of cauliflower mushroom found to grow mycelia in clamp at approximately 50%. The growth speed of mycelia was $0.507{\mu}m/min$ and 2nd mycelia grown similar speed to mother mycelia at parallel with mother mycelia after growth speed at $0.082{\mu}m/min$. The formation of clamp made small clamp for 5 hours after shown transfer of electrolyte in mycelia inside. The septum formation started after 3 hours and then finally completed after 2 hours. In this study, strain of cauliflower mushroom verified resistance of Trichoderma, genetic diversity and characteristic of mycelia growth. Therefore, basic knowledge of cauliflower mushroom will improve and further contribute to development of mushroom industry.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.217-223
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• 2008

Amylose and protein contents are important traits determining the edible quality of rice, especially in East Asian countries. Near-Infrared Reflectance Spectroscopy (NIRS) has become a powerful tool for rapid and nondestructive quantification of natural compounds in agricultural products. To test the practically of using NIRS for estimation of brown rice amylose and protein contents, the spectral reflectances ($400{\sim}2500\;nm$) of total 9,483 accessions of rice germplasm in Rural development Administration (RDA) Genebank ere obtained and compared to chemically determined amylose and protein content. The protein content of tested 119 accessions ranged from 6.5 to 8.0% and 25 accessions exhibited protein contents between 8.5 to 9.5%. In case of amylose content, all tested accessions ranged from 18.1 to 21.7% and the grade from 18.1 to 19.9% includes most number of accessions as 152 and 4 accessions exhibited amylose content between 20.5 to 21.7%. The optimal performance calibration model could be obtained from original spectra of brown rice using MPLS (Modified Partial Least Squares) with the correlation coefficients ($r_2$) for amylose and protein content were 0.865 and 0.786, respectively. The standard errors of calibration (SEC) exhibited good statistic values: 2.078 and 0.442 for amylose and protein contents, respectively. All these results suggest that NIR spectroscopy may serve as reputable and rapid method for quantification of brown rice protein and amylose contents in large numbers of rice germplasm.

• Research in Plant Disease
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• v.29 no.4
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• pp.331-344
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• 2023

More than a year has passed after the 6th edition of 'List of Plant Diseases in Korea (LPDK)' was published in April 2022. The 6.1st edition (2023) of List of Plant Diseases in Korea was made by correcting errors found in the 6th edition of list and adding new diseases reported after the 6th edition. There were 397 corrections from the 6th edition, most of which were simple spelling errors or minor issues. However, 12 diseases were deleted due to duplication or unclear literature proof, and 2 diseases had their diseases' common names changed. We added 158 diseases that were reported before 2021 but not included in the 6th edition, or reported after the 6th edition. After all, 146 diseases were added to the 6,534 diseases in the 6th edition, resulting in a total of 6,680 diseases in the 6.1st edition. Thirty host taxa were also added, increasing the number from 1,390 in the 6th edition to 1,420 in the 6.1st edition. Pathogens were also added to 62 taxa, from 2,400 in the 6th edition, bringing the total to 2,462 taxa in the 6.1st edition. Ultimately, the 6.1st edition (2023) of 'The List of Plant Diseases in Korea' contains 6,680 diseases caused by pathogens of 2,462 taxa on 1,420 hosts. The 6.1st edition is not printed as a book, but is provided through the online 'List of Plant Diseases in Korea' (https://genebank. rda.go.kr/kplantdisease.do).

• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.215-225
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• 1996

Aseptic meningits, an acute inflammation of the meninges, is a common illness during childhood. Virus is the most important cause of aseptic meningitis. Especially enterovirus causes approximately above 85% of all cases of aseptic meningitis. In 1993, there was a big epidemic of aseptic meningitis by ECHO 9 and ECHO 30 viruses. And ECHO 3 virus was isolated as a causative agent of aseptic meningitis in 1994. This study was aimed to detect the causative agent of aseptic meningitis in 1995 and to analyze the 5'-noncoding region which was used to detect virus. Virus was isolated from 87 stools and cerebrospinal fluid specimens of the patients by cultured RD and HEp-2 cell. Neutralizing antibody tests using enterovirus serum pool were performed on the specimens with cytopathic effect. 3 of ECHO 7 viruses and 5 of Coxsackie B3 viruses were isolated from stool specimens and 1 of ECHO 7 and Coxsackie B3 mixed type was confirmed from cerebrospinal fluid specimens. RNA was isolated from the culture supernatants of infected cells and general primers were selected in highly conserved part of the 5'-noncoding region of the enteroviral genome for RT-PCR. PCR product from this virus showed a 152bp band on gel electrophoresis. Sequence of obtained DNA was compared with prototype sequences by accessing to the Genebank database. 5'-noncoding region of isolated Coxsackie B3 virus, which has point mutations in nucleotide sequence positions 493, 497, 502, 523, was closely related to that of polio virus type 1, Mahoney strain. In case of isolated ECHO 7 virus, nucleotide has been changed from cytosine to thymine at position 581 and from thymine to cytosine at position 583. We concluded the causative agents of the outbreak of aseptic meningitis during June to July in 1995 were both ECHO 7 and Coxsackie B3 virus, and the primer used in this study could allow a rapid diagnosis of enteroviruses by PCR.