• 약학회지
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• 제54권3호
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• pp.151-156
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• 2010

Cytotoxicity of cadmium on NIH 3T3 fibroblasts was utilized in order to discover antitoxic compound in Galgeuntang extract in this study. Treatment groups were chosen as follows; control (medium only), $MTT_{50}$ group and five experimental groups. MTT {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} method was performed to evaluate the cytotoxicity of cell organelles and $IC_{50}$ was also measured. Accordingly we have examined the detoxification effects of Galgeuntang extract on cadmium-treated NIH 3T3 fibroblasts to observe morphological changes by the light microscopy. Galgeuntang extract showed cytoprotective effects on cadmium-induced cytotoxicity. Furthermore, Galgeuntang showed a dose-dependency in detoxication. The phenolic content of Galgeuntang ethanol extract was higher than that of water content. These results suggest that Galgeuntang extract may be used as a cytoprotective agent against cadmium (II)-mediated cytotoxicity.

• 한국한의학연구원논문집
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• 제15권1호
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• pp.79-83
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• 2009

In this study, we investigated the single dose toxicity and safety about water-extracted Galgeuntang. To evaluate single dose toxicity, 20 male and 20 female ICR mice were observed survival rates, general toxicity, changes of body weight for 14 days after single oral administration of 0 (control group), 1250, 2500 and 5000mg/kg Galgeuntang. And after 14 day, We observed autopsy. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 mg/kg). LD50 of Galgeuntang might be over 5000mg/kg and it is very safe to ICR mice.

• Advances in Traditional Medicine
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• 제8권1호
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• pp.32-38
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• 2008

Galgeuntang water extract exhibited a strong free radical scavenging activity and reducing power determination. However, a gradual increase in the free radical scavenging activity and reducing power determination was obtained with increasing concentrations. The highest radical scavenging activity was shown by the water extract from Galgeuntang (116.93 ${\mu}g$/mL) and the water extract from Cinnamonum cassia Presl. (95.01 ${\mu}g$/mL). These results of phenolic and flavonoid contents of the extracts indicated that the strong radical scavenging activity of the Cinnamonum cassia Presl. extract might be in part due to the phenolic compounds. The correlation coefficient between TPC and DPPH ($r^2$ = 0.9312), TFC and DPPH ($r^2$ = 0.9677), showed positive correlation among total phenolic/flavonoid contents and antioxidant activity. These results suggest that Galgeuntang has a potential antioxidant activity.

• Korean Journal of Acupuncture
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• 제39권2호
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• pp.34-42
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• 2022

Objectives : Parkinson's disease (PD) is a neurodegenerative disease caused by dopaminergic neuronal death in the substantia nigra pars compacta. PD is known to be linked with mitochondrial dysfunction and increased oxidative stress. In this study, anti-cytotoxic and anti-oxidative effect of 12 herbal formulae were compared. Methods : According to experts' advice, 12 types of herbal formulae (Gamisoyosan, Galgeuntang, Galgeunhaegitang, Banhabaekchoolcheonmatang, Bojungikgitang, Boheotang, Sihogyejitang, Sihosogantang, Sihocheonggantang, Ojeoksan, Cheongsanggyeontongtang and Palmultang) were selected from 56 types of herbal formulae covered by the National Health Insurance Service in Korea. To detect anti-oxidative effect, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was performed, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to detect anti-cytotoxic effect of 12 herbal formulae using SH-SY5Y human neuroblastoma cells. Results : In DPPH assay, anti-oxidant activity was increased in a dose-dependent manner and half maximal inhibitory concentration was highest in the order of Galgeuntang, Gamisoyosan, Galgeunhaegitang, Ojeoksan, Palmultang, Sihogyejitang, Sihosogantang, Cheongsanggyeontongtang, Sihocheonggantang, Bojungikgitang, Boheotang and Banhabaekchoolcheonmatang. In MTT assay, concentration of 80% cell survival was highest in the order of Sihosogantang, Cheongsanggyeontongtang, Sihocheonggantang, Sihogyejitang, Bojungikgitang, Galgeuntang, Ojeoksan, Boheotang, Palmultang, Galgeunhaegitang, Banhabaekchoolcheonmatang and Gamisoyosan. Formulae with more than 50% DPPH radical scavenging activity at concentrations for 80% cell survival were Sihosogantang, Cheongsanggyeontongtang, Sihogyejitang, Galgeuntang and Sihocheonggantang. Conclusions : Sihosogantang, Cheongsanggyeontongtang, Sihogyejitang, Galgeuntang and Sihocheonggantang extracts can be candidate medicines for PD, but the effect should be validated in PD models.

• 대한한의학원전학회지
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• 제23권1호
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• pp.47-58
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• 2010

"Sanghanron(傷寒論)" can be approached through the understanding of the formulas. It is very important to correlate the efficacy of drugs and pathogenesis of syndrome. In this sense, the author examines the Paeoniae Radix Alba's efficacy. The Paeoniae Radix Alba[芍藥] used in Gyejitang(桂枝湯), Galgeuntang(葛根湯), Sogeonjungtang (小建中湯), Jagyakgamchotang(芍藥甘草湯) all have a similar effect. The Paeoniae Radix Alba supplies the tendon and blood meridian with bodily fluids, so it treats the stiffness and pain of the head and neck[頭項强痛], stiffness of the neck and back[項背强几几] and acute pain in the abdomen[腹中急痛] etc. Meanwhile, the Paeoniae Radix Alba treats difficulty in urination. It moistens the path to the excretion of urine included among drugs which increase digestion and transformation.

• 한국식품과학회지
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• 제43권5호
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• pp.655-658
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• 2011

갈근탕을 이용한 유산균 발효에 적합한 균주를 선발하기 위하여 3종의 Lactobacillus균을 이용하여 발효시킨 갈근탕의 특성을 분석해 본 결과, 발효에 의한 유기산의 생성으로 3종의 발효 갈근탕 모두 pH가 3.8 이하로 저하되었다. 또한 각 Lactobacillus들에 의한 갈근탕의 지표물질 중 하나인 isoflavone 배당체 daidzin의 비배당화 활성을 평가한 결과 L. plantarum KFRI 144균주가 89.9%의 가수분해율을 보여 가장 높은 활성을 나타내었다. 이러한 결과로부터 L. plantarum KFRI 144균주는 갈근탕 약효성분의 생체이용률을 높일 수 있는 기능성 발효 갈근탕 제조의 starter 균주로 사용될 수 있을 것으로 기대된다.

• 농업과학연구
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• 제33권1호
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• pp.25-34
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• 2006

본 연구는 당밀의 첨가수준이 갈근탕박 사일리지의 품질에 미치는 영향을 조사하기 위하여 두가지 실험을 실시하였다. 실험 I에서는 당밀의 첨가수준이 갈근탕박 사일리지의 품질에 미치는 영향을 조사하기 위하여 0, 0.5 및 1.0%의 당밀 첨가수준에 3반복의 실험을 실시하였다. 실험 II에서는 우리나라 재래산양의 갈근탕박 사일리지의 기호성을 측정하였다. 본 실험에서 얻어진 결과를 요약하면 실험 I에서, 당밀 1.0% 첨가구가 0.5% 첨가구 및 대조구보다 유산의 함량이 유의하게(p<0.05) 높았고, pH와 낙산함량은 유의하게 낮았다. 또한 사일리지의 총미생물수(total microbial cell)는 당밀 첨가구가 유산균 배지인 MRS에서는 많게 나타났고, 곰팡이 배지인 PDA에서는 적은 결과를 나타내었다. 그리고 in vitro 건물 소실율에 있어서도 당밀 첨가수준이 증가할수록 증가하는 경향이었다. 재래산양을 사용한 실험 II에서는 갈근탕을 비롯한 모든 처리구에서 30분 및 6시간 동안의 섭취량은 유의한 성적은 아니지만 당밀 첨가수준이 증가할수록 많아지는 경향을 나타내었다. 이상의 결과를 종합하면 갈근탕박은 열탕처리를 거침으로 인하여 수용성 탄수화물이 부족할 것으로 사료되는 바, 이에 대하여 수용성탄수화물을 보충하는 방법으로 당밀을 첨가하여 사일리지를 제조하는 것은 매우 유용한 수단이라고 생각되며, 이로 인하여 한약제박 사일리지의 보존성도 향상된다고 하겠다.

• 대한한의학방제학회지
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• 제28권1호
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• pp.91-115
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• 2020

Objective : This study's purpose was to review the clinical studies of Galgeun-tang. Method : We searched papers about Galgeun-tang using KISS, RISS, OASIS, PUBMED and J-stage. The key words we used were "Galgeuntang", "Kakkonto", "Ge gen tang", and "Pueraria Decoction". Papers not matched with inclusion criteria were excluded. Results : Until today, there have been 223 studies on Galgeun-tang. Of these, 25 studies were classified as clinical research papers. There were 4 cases of fever, 5 cases of inflammation on respiratory system, 3 cases of head, neck and shoulder disorder, 2 cases of diarrhea, 6 cases of pharmacokinetics and interaction, 5 cases of side effect. Conclusion : It can be seen that Galgeun-tang has established the basis for application to the purpose of treating fever (common cold, influenza), inflammation on respiratory system (nasal obstruction, maxillary sinus retention cyst, mucoid pseudomonas aeruginosa chronic lower respiratory tract infection), head, neck and shoulder disorder (temporomandibular disorders, shoulder stiffness, tetanus), and diarrhea. On the other hand, considering 4 cases of side effect on drug eruption, caution should be exercised when observing the progress of the patient taking Galgeun-tang.

• 대한한의학원전학회지
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• 제23권4호
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• pp.1-11
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• 2010

"Gyeongbangsilheomrok(經方實驗錄)" is a book that was written for the propagation of the thought of Gyeongbang(經方) theory in the early 20th century. Gyeongbang means the medicine in "Sanghanron(傷寒雜病論)" which was written by Jangjonggyeong(張仲景), who is known as a great doctor of ancient times in China. Gyeongbang had worked as a good model of medicine for a long time, but as time goes by, there appeared some physicians pointing out the limit of Gyeongbang and trying to overcome it. Through the effort like this, there gradually had appeared so many physicians carrying out the medical treatment which was getting out of the boundary of Gyeongbang. And There also had appeared a group of physicians, called Gyeongbangpa(經方派), opposing the opinion like this and defending the academic value of Gyeongbang. "Gyeongbangsilheomrok" had been estimated as a meaningful achievement of Gyeongbangpa tradition, and also had been regarded as a good book comprising copious basic theory about Bangje(方劑). The most significant assertion in this book is that it explained the Onbyeong(溫病) theory as a component itself in "Sanghanron(傷寒論)", which had been argued by many other medicians thinking that "Sanghanron" did not cover the treatment of Onbyeong. In regarding to this problem, "Gyeongbangsilheomrok" argued that the concept of Onbyeong in "Sanghanron" belongs to the category of Taeyangbyeong(太陽病) and also suggested that Galgeuntang(葛根湯) would be the main recipe for Onbyeong.

• 한국환경과학회지
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• 제17권1호
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• pp.129-133
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• 2008

This study is an endeavor to evaluate the safety of medicines from heavy metals, prescribed on the basis of herbal medicinal system and oriental medical prescription which are circulated much recently. For that, three globular types, four extract granular types and four liquid types of herbal medicine were bought to compare and analyze the content of heavy metals, such as As, Pb, Cd and Hg, which are harmful to human body. The concentration of Pb was found to be 0.552 ppm in Sachiltang, 2.552 ppm in Anjungjogiwhan and 1.735 ppm in Cheongsangbohwawhan in case of pill type herbal medicine, and liquid type herbal medicine, Maekmundongtang was 0.002 ppm, Galgeuntang was 0.003 ppm, Sangwhatang was 0.004 ppm, 20jeon Daebotang was 0.0185 ppm. And the concentration of Pb was found to be 0.322 ppm in Banhasasimtang, 0.47 ppm in Eungjosan, 0.29 ppm in Yukmijihwangtang, 0.64 ppm in Socheongryongtang in case of granular type. It was found that the liquid types herbal medicines were relatively safer than three pill types of, four granular types of and four liquid types herbal medicines were tested for concentration of heavy metals. It is considered that is required in the stage of raw material treatment, manufacturing and packaging because those herbal medicines are directly taken in and absorbed into human body through the final treatment process.