YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Cytoprotective Effect of Galgeuntang Extract on Cadmium-Induced Cytotoxicity

갈근탕 추출물의 카드뮴 독성에 대한 세포 보호효과

  • Park, So-Yun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Baek, Jong-Min (R&D Center, BRNScience Co. LTD., Seoul National University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 박소윤 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백종민 (서울대학교 비알엔사이언스 연구개발부) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Received : 2009.09.24
  • Accepted : 2010.03.20
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cytotoxicity of cadmium on NIH 3T3 fibroblasts was utilized in order to discover antitoxic compound in Galgeuntang extract in this study. Treatment groups were chosen as follows; control (medium only), $MTT_{50}$ group and five experimental groups. MTT {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} method was performed to evaluate the cytotoxicity of cell organelles and $IC_{50}$ was also measured. Accordingly we have examined the detoxification effects of Galgeuntang extract on cadmium-treated NIH 3T3 fibroblasts to observe morphological changes by the light microscopy. Galgeuntang extract showed cytoprotective effects on cadmium-induced cytotoxicity. Furthermore, Galgeuntang showed a dose-dependency in detoxication. The phenolic content of Galgeuntang ethanol extract was higher than that of water content. These results suggest that Galgeuntang extract may be used as a cytoprotective agent against cadmium (II)-mediated cytotoxicity.

Keywords

References

  1. Nriagu, J. O. and Pacyna, J. M. : Quantitative assessment of worldwide contaminationn of air, water and soils by trace metals. Nature 333, 134 (1988). https://doi.org/10.1038/333134a0
  2. World Health Organization.: Environmental Health Criteria 134, Cadmium, Geneva, Switzerland (1992).
  3. Jin, T. and Nordberg, G. F. : Acta Pharmacol. Ed. Toxicol. In Vitro, p. 137 (1983).
  4. Kostonis, F. N. and Klaassen, C. D. : Toxicity and distribution of cadmium administered to rats at sublethal doses. Toxicol. Appl. Pharmacol. 41, 667 (1977). https://doi.org/10.1016/S0041-008X(77)80020-3
  5. Sillen, L. and Martel, A. : Stability constants of Metal-ion complexes, compiled for IUPAC, London. Chem. Soc. 6, 67 (1964).
  6. Choi, H. J., No, Y. J., Kang, J. I. and Baek, S. H. : The inhibitory effects of quercetin from Houttuynia cordata against cadmiuminduced cytotoxicity. J. Pharm. Soc. Korea 53, 12 (2009).
  7. Lee, J. H. Shin, S. W. and Baek, S. H. : Optimum concentration of the Cd(II)-quercetin complexation reaction. J. Pharm. Soc. Korea 53, 235 (2009).
  8. Lee, J. H., Shin, S. W. and Baek, S. H. : Complexation studies for cadmium (II) with quercetin and (+)-catechin. J. Pharm. Soc. Korea 53, 342 (2009).
  9. Ji, X., Tan, B. K., Zhu, Y. C., Linz, W. and Zhu, Y. Z. : Comparison of cardioprotective effects using ramipril and DanShen for the treatment of acute myocardial infarction in rats. Life Sci. 73, 1413 (2003). https://doi.org/10.1016/S0024-3205(03)00432-6
  10. Shin, J. M., Shin, S. W., Oh, H. J., Oh, H. K. and Baek, S. H. : Antioxidant activity of the extracts from Pueraria thunbergiana Benth. J. Cosm. Pub. Health 2, 136 (2006).
  11. Shin, J. M., Kim, Y. O. and Baek, S. H. : Free radical scavenging activity and kinetic behavior of the Galgeuntang water extract. Orien. Pharm. Exp. Med. 8, 32 (2008). https://doi.org/10.3742/OPEM.2008.8.1.032
  12. Ashmore, C. R., Lee, Y. B. and Kim, Y. S. : Antioxidantand meat products. J. Food Sci. 51, 20 (1996).
  13. Oh, M. J., Lee, K. S., Son, H. Y. and Kim, S. Y. : Antioxidative components of Pueraria root. Kor. J. Sci. Technol. 22, 793 (1995).
  14. Han, S. H., Kim, J. B., Min, S. G. and Lee, C. H. : The effects of Puerariae Radix catechins administration on liver function in carbon tetrachloride-treated rats. Kor. J. Soc. Food Nutr. 24,713 (1995).
  15. Lee, J. S., Lee, K. H. and Jeong, J. H. : Effects of extracts of Puerariae Radix on lipid metabolism in rats fed high fat diet. J. Kor. Soc. Food Sci. Nutr. 28, 218 (1999).
  16. Shin, J. M., Yu, H. H., Lee, H. O., Lee, M. H. and Baek, S. H. : Antimicrobial and antioxidative effects of isoflavonoids on skin microbial inflammation pathogens. J. Pharm. Soc. Korea 50, 15(2006).
  17. Mosmann, T. : Rapid colorimetric assays for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55 (1983). https://doi.org/10.1016/0022-1759(83)90303-4
  18. Sinkard, K. and Singleton, V. L. : Total phenol analysis: Automation and comparison with manual methods. Am. J. Enol. Viticul. 28, 49 (1977).
  19. Dinis, T. C. P., Madeira, V. M. C. and Almeida, L. M. : Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315, 161 (1994). https://doi.org/10.1006/abbi.1994.1485
  20. 채영암, 구자옥, 서학수 : 이영만. 기초생물통계학 제 9장 직선회귀, 학문사, 서울 p. 179 (1991).
  21. Young, H. T., Choi, H. J. and Baek, S. H. : Inhibitory effects of leachianone A from Sophora flavescens Ait. against cadmium induced cytotoxicity. Kor. J. Orien. Physiol. Pathol. 22, 1163(2008).
  22. Lim, J. Y., Yang, H. O., Oh, H. J., Lim, J. A. and Baek, S. H. : The repaired effect of the crude extracts from Cornus officinalis in NIH 3T3 fibroblasts against cadmium-induced cytotoxicity. J. Cosm. Pub. Health 1, 14 (2005).
  23. Lee, M. H., Lim, H. Y., Kim, Y. H., Oh, H. J. and Baek, S. H. : The Inhibition effect of the crude extracts from Pueariae thunbergiana Beth. against cadmium-induced cytotoxicity. J. Cosm. Pub. Health 1, 19 (2005).
  24. Kostyuk, V. A., Potapovich, A. I., Vladykovskaya, E. N., Korkina, L. G. and Afanas'ev, I. B. A. : Influence of metal ions on flavonoid protection against asbestos-induced cell injury. Arch. Biochem. Biophys. 385, 129 (2001). https://doi.org/10.1006/abbi.2000.2118
  25. Lapouge, C. and Cornard, J. P. : Time dependent density functional theory studey of electronic absorption properties of lead (II) complexes with a series of hydroxyflavones. J. Phys. Chem. A 109, 6752 (2005). https://doi.org/10.1021/jp0525235
  26. Cornard, J. P., Dangleterre, L. and Lapouge, C. : Computational and spectroscopic characterization of the molecular and electronic structure of the Pb(II)-quercetin complex. J. Phys. Chem. A 109, 10044 (2005). https://doi.org/10.1021/jp053506i
  27. Cornard, J. P. and Merlin, J. C. : Spectroscopic and structural study of complexes of quercetin with Al(III). J. Inorg. Biochem. 92, 19 (2002). https://doi.org/10.1016/S0162-0134(02)00469-5
  28. Lee, J. T., Lee, K. N., Lee, C. W., Chun, H. J., You, I. S., Lim, J. A. and Baek, S. H. : The inhibitory effects of quercitrin from Houttuynia cordata against cadmium induced cytotoxicity (VII). J. Kor. Chem. Soc. 47, 175 (2003). https://doi.org/10.5012/jkcs.2003.47.2.175