YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Simultaneous Analysis of Bioactive Metabolites from Caulis Lonicera japonica by HPLC-DAD-ion trap-MS

HPLC-DAD-ion trap-MS를 이용한 인동 생리활성 물질의 동시분석

  • Ryu, Sung-Kwang (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Won, Tae-Hyung (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Shin, Jong-Heon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • 유성광 (서울대학교 약학대학 천연물과학연구소) ;
  • 원태형 (서울대학교 약학대학 천연물과학연구소) ;
  • 강삼식 (서울대학교 약학대학 천연물과학연구소) ;
  • 신종헌 (서울대학교 약학대학 천연물과학연구소)
  • Received : 2009.10.21
  • Accepted : 2010.03.20
  • Published : 2010.06.30
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Abstract

A high-performance liquid chromatography (HPLC) with DAD detector and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of coniferin (1), loganic acid (2), demethylsecologanol (3), sweroside (4) and loganin (5) from caulis Lonicera joponica. The optimal chromatographic conditions were obtained on an ODS column ($5{\mu}m$, $4.6{\times}150mm$) with the column temperature $35^{\circ}C$. The mobile phase was composed of (A) water with 0.1% formic acid and (B) methanol with 0.1% formic acid using a gradient elution, the flow rate was 0.3 ml/min. Detection wavelength was set at 254 nm. All calibration curves showed good linear regression ($r^2$>0.998) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and interday variations of 0.16~3.28% and 0.14~1.99%, respectively, and the overall recoveries of 99.39~105.89% for the five compounds analyzed. The verified method was successfully applied to quantitative determination of the two types (phenolic compounds and iridoids) of bioactive compounds in 24 commercial caulis L. japonica samples from different markets in Korea and China. The analytical results demonstrated that the contents of the five analytes vary significantly with sources.

Keywords

References

  1. Li, H. J., Zhang, Z. Y. and Li, P. : Comparative study on volatile oils in flower and stem of Lonicera japonica. Journal of Chinese Medicinal Materials (Zhongyaocai) 25, 476 (2002).
  2. 진재인 : 도설한방의학대사전(중국약학대전) I, 강담사, p. 160 (1982).
  3. Chinese Pharmacopoeia Committee. the chinese pharmacopoeia 2005 edition, part . Chemical Industry Press: beijing, p. 130 (2005).
  4. Mathad, V. T., Raj, K., Bhaduri, A. P., Sahai, R., Puri, A., Tripathi, L. M. and Srivastava, V. M. L. : Studies on the profile of immunostimulant activities of modified iridoid glycosides. Bioorganic Medicine and Chemistry 6, 605 (1998). https://doi.org/10.1016/S0968-0896(98)00011-X
  5. Kumarasamy, Y., Nahar, L., Cox, P. J., Jaspars, M. and Sarker, S. D. : Bioactivity of secoiridoid glycosides from Centaurium erythraea. Phytomedicine 10, 344 (2003). https://doi.org/10.1078/094471103322004857
  6. Marinova, E. M. and Yanishlieva, N. V. : Antioxidant activity and mechanism of action of some phenolic acids at ambient and high temperatures. Food Chemistry 81, 189 (2003). https://doi.org/10.1016/S0308-8146(02)00411-9
  7. Chang, C.-W., Lin, M.-T., Lee, S.-S., Liu, K. C. S. C., Hsu, F.-L. and Lin, J.-Y. : Differential inhibition of reverse transcriptase and cellular DNA polymerase-a activities by lignans isolated from Chinese herbs, Phyllanthus myrtifolius Moon, and tannins from Lonicera japonica Thunb and Castanopsis hystrix. Antiviral Res. 27, 367 (1995). https://doi.org/10.1016/0166-3542(95)00020-M
  8. Chen, J. and Ma, S. C. : Extraction, separation and identification of loganin and sweroside from Caulis Lonicerae japonicae. Chinese Journal of Modern Applied Pharmacy (Zhongguo Xiandaiyingyongyaoxue Zashi) 23, 199 (2006).
  9. Wu, X. F., Li, Y. X., Hou, H. E. and Wei, W. : Determination of chlorogenic acid in the branch of Lonicera japonica. Journal of Chinese Medicinal Materials (Zhongyaocai). 19, 69 (1996).
  10. Li, Y. X. : Determination of the chlorogenic acid and chemical compounds of flavones contents in the branch of Lonicera japonica Thunb. HeNan Science (Henan Kexue) 16, 63 (1998).
  11. Lin, R. Y. and He, P. X. : Determination of chlorogenic in the stem of Lonicera japonica. Henan Science (Henan Kexue) 24, 38 (2006).
  12. Chen, J. and Ma, S. C. : HPLC determination of sweroside and loganin in Caulis Lonicerae. Chinese Journal of Pharmaceutical Analysis (Yaowu Fenxi Zazhi) 25, 1451 (2005).