• Macromolecular Research
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• v.10 no.3
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• pp.158-167
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• 2002

In order to endow with new bioactive functionality from small intestine submucosa (SIS) powder as natural source to poly (L-lactide) (PLA) and poly (lactide-co-glycolide) (PLGA) synthetic biodegradable polymer, porous SIS/PLA and SIS/PLGA as natural/synthetic composite scaffolds were prepared by means of the solvent casting/salt leaching methods for the possibility of the application of tissue engineered bone and cartilage. A uniform distribution of good interconnected pores from the surface to core region was observed the pore size of 40~500 ${\mu}{\textrm}{m}$ independent with SIS amount using the solvent casting/salt leaching method. Porosities, specific pore areas as well as pore size distribution also were almost same. After the fabrication of SIS/PLA hybrid scaffolds, the wetting properties was greatly enhanced resulting in more uniform cell seeding and distribution. Five groups as PGA non-woven mesh without glutaraldehyde (GA) treatment, PLA scaffold without or with GA treatment, and SIS/PLA (Code No.3 ; 1 : 12 of salt content, (0.4 : 1 of SIS content, and 144 ${\mu}{\textrm}{m}$ of median pore size) without or with GA treatment were implanted into the back of nude mouse to observe the effect of SIS on the induction of cells proliferation by hematoxylin and eosin, and von Kossa staining for 8 weeks. It was observed that the effect of SIS/PLA scaffolds with GA treatment on bone induction are stronger than PLA scaffolds, that is to say, in the order of PLA/SIS scaffolds with GA treatment > PLA/SIS scaffolds without GA treatment > PGA nonwoven > PLA scaffolds only with GA treatment = PLA scaffolds only without GA treatment for the osteoinduction activity. The possible explanations are (1) many kinds of secreted, circulating, and extracellular matrix-bound growth factors from SIS to significantly affect critical processes of tissue development and differentiation, (2) the exposure of SIS to GA resulted in significantly calcification, and (3) peri-implant fibrosis due to covalent bonding between collagen molecule by crosslinking reaction. In conclusion, it seems that SIS plays an important role for bone induction in SIS/PLA scaffolds for the application of tissue engineering area.

• Proceedings of the Membrane Society of Korea Conference
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• 1994.10a
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• pp.32-33
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• 1994

Poly(vinyl alcohol)(PVA) membranes crosslinked with Glutaraldehyde (GA) were prepared for the separation of acetic acid-water mixtures. For the preparation of the crosslinked membranes, dry PVA films were immersed for 2 days at 40$\circ$C in reaction solutions which contain different content of GA solution(25% GA in water), aceton and a catalyst, HCl. IR spectroscopy was employed to characterize the crosslinking reaction between hydroxyl groups in PVA and aldehyde groups in GA. Swelling measurements of the crosslinked membranes were carried out in both water and acetic acid to investigate the crosslinking density and swelling behaviour of the membranes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.141-148
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• 1992

Field experiment was carried out to investigate the effect of plant growth regulator (PGR); IAA, GA, Kinetin to regrowth of sorghum and pearlmillet according to variety and plant growth stage. Kinetin application after cut increased tiller number and decreased dry weight of regrowth, but its application on sorghum stubble in water stress increased tiller number and leaf elongation rate, consequently increased regrowth dry weight. GA application reduced tiller production in both species, but tiller formation in pearlmillet was decreased more than in sorghum by promoting leaf elongation of old tiller. Nonstructural carbohydrate (NSC) of stubble during regrowth was consumed less at anthesis than at stem elongation stage because of senescence of tiller primordia. GA treatment reduced NSC content more than other PGR in both plant species, by consuming reserve NSC and stimulating rapid elongation of old tiller after cut. Dry matter increase during regrowth had high correlation with tiller number and tiller elongation a week after cut, while it did not have any correlation with NSC at cutting stage or with consumption of NSC during regrowth. Therefore, regrowth in sorghum and pearmillet must depend upon activity of tiller primordia more than upon amount of reserved NSC.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.344-352
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• 2002

This study was conducted to determine the optimum planting dates and density of one year old Bupleurum falcatum L. to improve its quality. Furthermore, the effect of cultural methods and plant growth regulators$(GA_3,\;IAA,\;Kinetin)$ on the quality of B. falcatum were also investigated. In this study, Jeongseon cultivar collected in Korea and Mishima cultivar introduced from Japan were used. Some of the results obtained are as follows. Jeongseon cultivar showed less stem branches and shoot weight compared to Mishima. However, Jeongseon cultivar showed tall plant height, high root fresh and dry weight, and high levels of SSa and TSS, but low SSc content than that of Mishima. Although fresh and dried root weight of both cultivars were not affected by plant growth regulators treatment. compared with the untreated one, increasement of TSS content were made by $GA_3$ 100 ppm, IAA 50 ppm or 10 ppm and kinetin 10 ppm treatment in Jeongseon cultivar, and by $GA_3$ 10 ppm and IAA 100 ppm or 10ppm treatment in Mishima cultivar applied on June 1.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.552-560
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• 2010

$GA_{4+7}$+BA, a plant growth regulator for induction of feathering in young apple tree and increasing fruit size, was applied by various methods on 'Gala'/M.9 apple trees in high density orchard for 4 years to investigate its effect on fruit and shoot growth. $GA_{4+7}$+BA($80-300mg{\cdot}L^{-1}$) increased fruit length, fruit weight, and L/D ratio regardless of application methods, but it did not affect soluble solid content, acidity, leaf area, and chlorophyll. Seed number was not affected by $GA_{4+7}$+BA application, however, more immature seeds was observed in treated 'Gala' fruit. Shoot growth was increased when spraying $GA_{4+7}$+BA at tree crown but not affected when spraying at fruit directly. More significant fruit growth was observed when $GA_{4+7}$+BA was applied on the fruits between late of May and early of June when fruit cell division ended; however, high concentration of $GA_{4+7}$+BA resulted in lower fruit storability because of lower firmness. Hence, more attention should be paid when applying high concentration of $GA_{4+7}$+BA to small sized fruit cultivars like 'Gala'.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.32 no.6
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• pp.528-533
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• 2019

We investigated the growth of $(Al_xGa_{1-x})_2O_3$ thin films on c-plane sapphire substrates that were grown by mist chemical vapor deposition (mist CVD). The precursor solution was prepared by mixing and dissolving source materials such as gallium acetylacetonate and aluminum acetylacetonate in deionized water. The [Al]/[Ga] mixing ratio (MR) of the precursor solution was adjusted in the range of 0~4.0. The Al contents of $(Al_xGa_{1-x})_2O_3$ thin films were increased from 8 to 13% with the increase of the MR of Al. As a result, the optical bandgap of the grown thin films changed from 5.18 to 5.38 eV. Therefore, it was determined that the optical bandgap of grown $(Al_xGa_{1-x})_2O_3$ thin films could be effectively engineered by controlling Al content.

• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1467-1475
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• 2015

The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA₁, GA₃, GA₇, GA₈, GA₉, GA₁₂, GA₁₉, GA₂₀, GA₂₄, and GA₅₃), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2002.07a
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• pp.383-386
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• 2002

Polycrystalline Cu(In,Ga)Se$_2$(CIGS) thin-films were grown by co-evaporation on a soda lime glass substrate. In this paper the effects of the Se evaporation temperature on the properties of CuIn0.75Ga0.25Se2 (CIGS) thin films. Structure, surface morphology and optical properties of CIGS thin films deposited at various Se evaporation temperatures have been investigated using a number of analysis techniques. X-ray diffraction (XRD) analysis shows that CIGS films exhibit a strong <112> preferred orientation. As expected, at higher Se evaporation temperatures the films displayed a lower degree of crystallinity. The <112> peak was also enhanced and other CIGS peaks appeared simultaneously. These results were supported by experimental work using scanning electron microscopy When the Se evaporation temperature was increased, the average grain size also decreased together with a reduction Cu content. The Se evaporation temperature also had a significant inf1uence on the transmission spectra. Increasing the Se evaporation temperature, the cell efficiency was improved dramatically to 11.75% with Voc = 556 mV, Jsc = 32.17 mA/cm2 and FF = 0.66. The Se evaporation temperature is an important parameter in thin film deposition regardless of the deposition technique being used to deposit thin films

• Journal of the Korean Vacuum Society
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• v.17 no.1
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• pp.34-39
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• 2008

We have investigated the characteristics of $Al_{0.55}Ga_{0.45}N$/GaN heterostructures with and without low-temperature (LT) AlN interlayer grown by metalorganic chemical vapor deposition. The structural and optical properties were systematically studied by Rutherford backscattering spectroscopy (RBS), X-ray diffraction (XRD), optical microscopy (OMS), scanning electron microscopy (SEM), and photoluminescence (PL). The Al content (x) of 55% and the structural properties of $Al_xGa_{1-x}N$/GaN heterostructures were investigated by using RBS and XRD, respectively. We carried out OMS and SEM experiments and obtained a decrease of the crack network in $Al_{0.55}Ga_{0.45}N$ layer with LT-AlN interlayer. A two-dimensional electron gas (2DEG)-related PL peak located at ${\sim}3.437eV$ was observed at 10 K for $Al_{0.55}Ga_{0.45}N$/GaN with LT-AlN interlayer. The 2DEG-related emission intensity gradually decreased with increasing temperature and disappeared at temperatures around 100 K. In addition, with increasing the excitation power above 3.0 mW, two 2DEG-related PL peaks were observed at ${\sim}3.411$ and ${\sim}3.437eV$. The observed lower-energy and higher-energy side 2DEG peaks were attributed to the transitions from the sub-band level and the Fermi energy level of 2DEG at the AlGaN/LT-AlN/GaN heterointerface, respectively.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.50-55
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• 1999

To develop a new form of controlled release dosage for administering for indomethacin (IND), two formulations of IND-loaded nanoparticles were designed based on polysaccharide (guar) derivatives. Nanoparticles prepared by the dialysis method were characterized with respect to morphology, size distribution, drug content, and in vitro drug release. Morphological studies by scanning electron microscopy (SEM) indicated that guar acetate (GA) nanoparticles were spherical in shape and had a smooth surface. The particle size distributions of formulation I (40mg of GA) and formulation II (80mg of GA) were shown to be $250.78\pm185.13nm$ and $718\pm145.90nm$ in distilled water ($20$^{\circ}C$), respectively. The drug loading efficiencies of nanoparticles were approximately 26% and 31% for formulations I and II, respectively. The differential scanning calorimetry (DSC) results indicated that the IND was perfectly distributed within GA nanoparticles. We also found, from the X-ray diffractometry analysis, that a decrease in the degree of crystallinity of the drug occurred in the nanoparticles. No changes between the original IND and the released IND from GA nanoparticles were detected by FT-IR. Using guar acetate, it is possible to design nanoparticles which allow the controlled release of IND over an extended period of time.