• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1694-1699
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• 2011

The quality characteristics and physiological effects of chunggukjang fermented naturally (NF-c), with Bacillus subtilis-SKm (BS-c), with Bacillus subtilis HJ18-4 (BH-c), and with Bacillus subtilis KCCM 42923 (BK-c) were investigated. The characteristics of fermentation were determined by protease, ${\alpha}$-amylase and ${\gamma}$-GTP activities, and additionally the amounts of amino-type and ammonia-type nitrogens. BS-c showed the highest protease, ${\alpha}$-amylase, and ${\gamma}$-GTP activities, and also amino-type nitrogen content among the four types of chunggukjang. The ammonia-type nitrogen content in BS-c was similar to that of BK-c and NF-c. BH-c showed the lowest enzyme activities and amino-type and ammonia-type nitrogen content. BS-c, BH-c, BK-c, and NF-c showed a similar overall acceptability during sensory evaluation. BS-c also showed the strongest DPPH free radical scavenging and anti-proliferative activities in HT-29 human colon carcinoma cells. These results suggested that B. subtilis-SKm was suitable to be used as a starter to enhance the quality and effects of chunggukjang.

• The Korean Journal of Pharmacology
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• v.30 no.2
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• pp.153-165
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• 1994

In this study, we tested the influences of several ${\kappa}$ opioid ligands on the $[^3H]diprenorphine$ binding in rat and guinea pig cortex membrane preparations. Using paradigm to block ${\mu}\;and\;{\delta}$ opioid receptors with $DAMGO(1{\mu}M)$ and $DPDPE(1{\mu}M)$, $[^3H]diprenorphine$ labeled ${\kappa}$ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of $[^3H]diprenorphine$ binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the $[^3H]diprenorphine$ binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the $[^3H]diprenorphine$ binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the ${\kappa}_1$, and ${\kappa}_2$ receptor most effectively. We, also, examined the influence of Na ion and $GTP{\gamma}S$, a nonhydrolyzable guanine nucleotide analog, on the inhibition of $[^3H]diprenorphine$ binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of $GTP{\gamma}S$, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by $GTP{\gamma}S$, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of ${\kappa}$ opioid receptor, ${\kappa}_1$, and ${\kappa}_2$, showing different Ki values for various ${\kappa}$ opioid ligands, also, bremazocine possess the antagonistic property at ${\kappa}_2$ site which is dominant subtype of K receptor in rat cortex.

• Journal of Preventive Medicine and Public Health
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• v.27 no.4 s.48
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• pp.747-761
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• 1994

Workers' periodic health examination is the main tools used to manage the health problems of most workers in Korea. The most common health problem found in workers' periodic health examination is liver disorder. Liver disorder is also one of the most common health problems in general population and one of the leading causes of mortality in adult population. Regulation proposed by government (No. 207, Ministry of Labor, 1992) defines the criteria for selection of workers with the liver dysfunction for further evaluative examination and the examination items used for diagnosis of the workers with liver dysfunction. This study was designed to evaluate the proficiency of each examination items presently defined in Regulation and propose the optimal examination items for detection of the liver disorders found by workers' periodic health examination. Study subjects are 186 workers with abnormal liver function tests in screening examination of workers' periodic health examination. Questionnaire survey including past history of liver disorder, drinking history, height and weight was done. Physical examination by physician, routine test items defined by Regulation (SGOT, SGPT, $\gamma$-GTP, protein, albumin, total and direct bilirubin, alkaline phosphatase, $\alpha$-feto protein, HBsAg and anti-HBs), anti-HCV antibody test and liver ultrasonography were done. Results are as follows; 1. Result of evaluative examination utilizing only the items defined in Regulation was; There were 75 workers with suspected live. disorder(40.3%), 63 with no liver dysfunction (33.9%), 13 with suspected hepatitis B(7.0%), 10 workers with hepatitis B(5.4%), 10 workers with hepatitis B carrier state(5.4%), 10 with alcoholic liver disorders(5.4%), 5 with fatty liver(2.7%). When alternative diagnostic criteria applying additional examination items (drinking history, body mass index, anti-HCV antibody and ultrasonography) diagnosability of liver disorder was increased. When all four items were included, final results were; 23 workers (17.8%) with hepatitis B (10 carriers, 13 suspects and 10 hepatitis B), 10 (5.4%) with hepatitis C(4 carriers, 5 suspects and 1 hepatitis C), 13(7.0%) with alcoholic liver disorder, 45(24.2%) with fatty liver (40 suspects, 5 fatty liver), 410%) with suspected liver disorders and 44 (23.7%) with normal liver. 2. Of examination items defined by Regulation, only SGOT, SGPT, $\gamma$-GTP and HBsAg were significantly different in abnormal rate and mean value, and all other laboratory findings did not showed significant difference between two groups. Drinking history, body mass index and anti-HCV antibody test which are the items that authors included in this study, also showed significant difference between two groups. Utilization of body mass index (BMI) for abnormal liver function group in diagnosis of fatty liver had high specificity (97.6%) but sensitivity (22.3%) was low. Therefore we suggest that SGOT, SGPT, $\gamma$-GTP, HBsAg, alcohol drinking history, BMI and anti-HCV Ab were useful for diagnosis of liver disorders among worker's periodic health examination.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.47-51
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• 2004

Green tea, which is high in polyphenols, is thought to have hypocholesterolemic effects. The present study was performed to further elucidate the hypocholesterolemic actions of green tea, specially the catechin and (-)-epigallocatechin gallate (EGCG) for their effects on the diet-induced hypercholesterolemia in rats. Male Sprague-Dawley rats were fed with green tea-free diet (control), diets containing 4% green tea powder (GTP), 1.0% green tea catechin (catechin) or 0.5% epigallocatechin gallate (EGCG) for 7 wks. All diets that were provided green tea contained approximately 0.5% EGCG Hypercholesterolemia was induced by adding 1% cholesterol and 0.5% cholic acid to all diets. There were no differences in food intake among groups. The green tea treatments showed significant improvement in the serum levels of total cholesterol, LDL-cholesterol, triacylglycerides and atherogenic index in the following order; EGCG>Catechin>GTP (p<0.05). The serum HDL-cholesterol level was highest in the EGCG-treated group. The catechin or EGCG diet up-regulated by 5 times the enzyme activity of hepatic cholesterol 7$\alpha$ -hydroxylase (CYP7Al) compared to control diet (p<0.05). Hepatic CYP7Al mRNA level paralleled tile increases in the CYP7Al activity. These results suggest that the EGCG in the green tea may account for the hypocholesterolemic effect by the induction of CYP7Al gene expression.

• The Journal of Pediatrics of Korean Medicine
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• v.11 no.1
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• pp.183-204
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• 1997

In order to study the effects of Daebangpungtang on galactosamine induced hepatoxity in rats, the experimental rats divided five groups (Normal, Control, Sample A, B, C groups). Under the same condition, normal and control groups were administered normal saline for 16 days, control group was injected to abdomen with galactosamine at 8th day (800mg/kg). Sample A group was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) for 8 days and injected galactosamine(800mg／kg i.p) for the last day and was administered normal saline for 8 days. Sample B group was treated as same as group A for 8 days, and then was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) forfurther 8 days. Sample C group was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) for 16 days. The activity of GOT, GPT, ${\gamma}$-GTP, ALP, LDH and total bilirubin in serum were measured at 8th and last day. The results were obtained as follows: 1. The activity of serum GOT of the sample A group decreased effectively at the 8th day and sample B group decreased effectively at 16th day as compared with the control group. 2. The activity of serum GPT of the sample A group decreased effectively at the 8th and 16th day and sample B group decreased effectively at 16th day as compared with the control group. 3. The activity of serum ${\gamma}$-GTP of the sample B group decreased effectively at the 16th day as compared with the control group. 4. The activity of serum ALP of the sample A group increased respectively at the 8th and 16th day and sample B group decreased effectively at 16th day as compared with the control group. 5. The activity of serum LDH of the sample A, B groups decreased effectively at 16th day as compared with the control group. 6. The activity of serum total bilirubin of the sample A, B groups decreased effectively at 16th day as compared with the control group. 7. The activity of GOT, GPT, ${\gamma}$-GTP, ALP, LDH and total bilirubin in serum of the sample C group were analogous with thats of normal group.

• Journal of Preventive Medicine and Public Health
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• v.29 no.1 s.52
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• pp.113-131
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• 1996

This study was carried out to evaluate the relations among workers' general characteristics, work-related behaviors and health related behaviors in a steel industry with 1,134 workers in Pohang. The results were as follows; 1. The mean age of workers was 50 years old and working duration was 15 years and over. Most of them were married(94.5%) and had studied beyond high school (53.0%). They performed three shift work and most of them(63.0%) had experienced industial accidents. The frequency of noise and dust exposure was defined by a minimum of 6 hours per day, and workers complained about noise exposure(62.9%) and dust(55.6%). There were current smokers(67.7%), current drinkers(74.3%) and current exercising workers(32.3%) in the industry. The number of cigarette consumption in current drinkers was significantly high $(13.6{\pm}8.4\;pieces/day)$ and the alcohol consumption in current smokers was significantly high$(104.5{\pm}113.5\;g/wk)$. And the number of cigarette consumption of exercisers was significantly low and the alcohol consumption of exercisers was higher than non-exercisers. 3. The ratio of current smokers on frequent noise in exposed workers versus non-exposed workers was not significantly high but the current drinkers in frequent noise and dust exposed workers was significantly higher than non-exposed. 4. The normal levels of SGOT workers in non-smokers were significantly higher than in current smokers, and the normal levels of LFT (SGOT, SGPT, $\gamma-GTP$ workers in nondrinkers were significantly high. The normal levels of SBP and DBP workers in current smokers were not significantly high but were significantly high in non-drinkers. 5. The ratio of current smokers in voters unsatisfied with their job and working condition was higher than non-smokers, but the ratio of current drinkers in workers satisfied with their job and working condition were higher. 6. The significant factors for SGOT and $\gamma-GTP$ were age, the drinking amount and BMI. But the only significant factor for r-GTP was BMI. The significant factors for DBP were age, the alcohol consumption and BMI. And the significant factors for SBP were age, BMI.

• Journal of Acupuncture Research
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• v.29 no.3
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• pp.9-18
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• 2012

Objectives : This research was performed to investigate the effects of Rhei Radix et Rhizoma pharmacopuncture at Bi-Su($BL_{20}$) on weight change, food efficiency, concentration of serum lipid, liver function of rats fed high fat diet for 8 weeks. Methods : Subject groups were diveded into normal diet group(Normal), high fat diet group(Control), high fat diet and saline in $BL_{20}$ treated group(Saline), high fat diet and Rhei Radix et Rhizoma pharmacopuncture in $BL_{20}$ treated group(PT-1, 0.008mg/ml/g), high fat diet and Rhei Radix et Rhizoma pharmacopuncture in $BL_{20}$ treated group(PT-2, 0.021mg/ml/g), and high fat diet and Rhei Radix et Rhizoma pharmacopuncture in $BL_{20}$ treated group(PT-3, 0.042mg/ml/g). Pharmacopuncture was bilaterally treated at BL20 every 4 days, totally 14 times in 56 days. We observed the changes of body weight, food efficiency, ALT, AST, r-GTP, total cholesterol, triglyceride, HDL, LDL-cholesterol of each group. Results : 1. Body weight significantly decreased, as compared with the control group, in PT-1 from 2nd week to 8th week, in PT-2 from 4th week to 7th week. 2. Food efficiency decreased, as compared with the control group, in PT-1(0.05${\leq}$p<0.06). 3. HDL-cholesterol increased, as compared with the control group, in PT-1, PT-3, but not significantly. 4. In the changes of ALT, as compared with the control group, only in PT-1 group, the level of serum ALT decreased(0.05${\leq}$p<0.06). 5. As for the changes of r-GTP, as compared with control group, in every subject group, the levels of serum r-GTP significantly decreased. Conclusions : According to the above results, Rhei Radix et Rhizoma pharmacopuncture at $BL_{20}$ can act on body weight, food efficiency and liver function. It also does not seem to be injurious on liver function. To use Rhei Radix et Rhizoma pharmacopuncture for obesity, further studies on its underlying mechanism and optimal dosage might be needed.

• Journal of Ginseng Research
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• v.24 no.4
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• pp.168-175
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• 2000

Relatively little is known about the signaling mechanism of ginseng saponins (ginsenosides), active ingredients of ginseng, in non-neuronal cells. Here, we describe that ginsenosides utilize a common pathway of receptor-mediated signaling pathway in Xenopus oocytes: increase in intracellular $Ca^{2+}$ concentration via phospholipase C (PLC) and $Ca^{2+}$ mobilization. Ginsenosides induced a marked and robust artivation of $Ca^{2+}$-activated Cl- channels in Xenopus oocytes. The effect of ginsenosides was completely reversible, in a dose-dependent manner with EC$_{50}$ of 4.4 $\mu\textrm{g}$/mi, and specifically blocked by niflumic acid, an inhibitor of $Ca^{2+}$-activated Cl- channel. Intracellular injection of BAPIA abolished the effect of ginsenosides. Intracellular injection of GTP${\gamma}$S also abolished the effect of ginsenosides. The effect of gin senosides on $Ca^{2+}$-activated Cl- currents was greatly reduced by the intracellular injection of heparin, an IP$_3$ receptorantagonist or the pretreatment of PLC inhibitor. These results indicate that ginsenosides activate endogenous $Ca^{2+}$-activated Cl- channels via the activation of PLC and the release of $Ca^{2+}$ from the IP$_3$-sensitive intracellular store following the initial interaction with membrane component(s) from extracellular side. This signaling pathway of ginsenosides may be one of the action mechanisms for the pharmacological effects of ginseng.ts of ginseng.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.4 s.54
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• pp.311-321
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• 2005

Tea (Camellia sinenis) is a popular beverage consumed worldwide. Since green tea, mainly consumed in Asia, has various biological activities, green tea components became one of the most favorite candidates as a functional materials for cosmetics and functional foods. The biological activities of green tea for skin cue have been ranged from protection of epidermal cells to the stimulation of extracellular matrix (ECM) biosynthesis. Green tea polyphenols (GTPs), which are active ingredients of green tea, possess anti-inflammatory, anti-carcinogenic and immune potentiation properties as well as antioxidant. They also modulate intracellular signal transduction pathways. GTPs decrease ultraviolet (UV)-induced oxidative stress, thus suppress mitogen-activated protein kinase (MAPK) pathway and apoptosis in keratinocytes. In addition, GTPs prevent the Induction of inflammatory mediators, such as cyclooxygenase-2 (COX-2), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) by tumor necrosis factor alpha $(TNF{\alpha})$ or chemical treatment in keratinocytes. GTPs treatment protects from chemical-or UV-induced skin tumor incidence in animal experiment. Besides, GTPs stimulate keratinocyte differentiation and proliferation of normal and aged epidermal cells, resectively, and suppress matrix metalloproteinases (MMPs) release. According to the progress of formulation study, green tea components will be guaranteed materials for the more effective skin cue products.

• The Journal of Korean Medicine
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• v.30 no.3
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• pp.106-110
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• 2009

Objective : To study the Oriental Medicine-based strategies or therapeutics for chronic HBV infection. Methods : A chronic HBV carrier was treated with only oriental therapies. Then, serum biochemical parameters were serially chased, and change of HBV-DNA level was evaluated. Result : The biochemical indicators (AST, ALT, gamma-GTP, bilirubin) fluctuated during the treatment period. After one episode of drastic elevation of serum aminotransferase, HBV-DNA disappeared from the blood along with normalization of biochemical parameters within two years of beginning treatment. Conclusion : Oriental Medicine-based therapeutics could be an alternative strategy against chronic infection of HBV.