• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.574-579
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• 2014

A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific activity greater than 500 mg/L and 4,000 U/mg was obtained. Results at optimal pH and temperature showed recombinant lysozyme has higher lytic activity at pH 6.5 and $45^{\circ}C$. This study demonstrates the successful expression of recombinant lysozyme using the eukaryotic host organism P. pastoris paving the way for protein engineering. Additionally, this study shows the feasibility of subsequent industrial manufacture of the enzyme with this expression system together with a high purity scheme for easy high-yield purification.

• Journal of the Korean Society of Safety
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• 제25권5호
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• pp.33-38
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• 2010

Railway is one of popular transportation systems. Due to the safety and in time, the transportation by train is preferred to the others for a long time. However, accidents under railway construction get more serious problems than in case of the other system, because it is the largest mass transportation system. Especially, the railway construction is composed of different kinds of construction works such as civil, architect, electric and mechanic. It is closely/complicatedly connected with various workers, large machines and facilities, regulations etc. In this study the data on safety accidents are corrected during 6 years(2004~2009) and analyzed by time(year, month, week, day, a.m or p.m), place, field, type etc. Results shows that there exists remakable trends on accidents, so efficient treatments should be prepared considering this trend.

• Journal of the Korean Institute of Telematics and Electronics A
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• 제30A권8호
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• pp.72-80
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• 1993

We fabricated 0.3um gate length inverse-T gate MOS(ITMOS) and conventional lightly doped drain oxide spacer MOS(LDDMOS), and studied electrical characteristics for comparison. Threshold voltage of 0.3um gate length device was 0.58 V for ITMOS and 0.6V for LDDMOS. Measured subthreshold characteristics showed a slope of 85mV/decades for both ITLDD and LDDMOS. Maximum transconductance at V S1ds T=V S1gs T=3.3V was 180mS/mm for ITMOS and 163mS/mm for LDDMOS respectively. GIDL current was observed to be 0.1pA/um for ITOMS and 0.8pA/um for LDDMOS. Substrate current of ITMOS as a function of drain current was found to be reduced by a foactor of 2.5 compared with that of LDDMOS.

• Journal of the Korean Institute of Telematics and Electronics S
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• 제34S권11호
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• pp.138-147
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• 1997

The ultrasonic images are corrupted by the granular pattern noise - a speckle noise. The speckle exist in the type of coherent imaging systems, and the speckle is the signal independent and multiplicative noise. In this paepr, we derive two filters using the gradient and symmetry. One is a noise suppression filter which removes noise while preserves the edges. It is named the ASRF-GS (Adaptive Speckle Removal Filer - Gradient and Symmetry). And the other is a edge detection filter which obtains the thin edge map, called the EDUGS(Edge Detection Using Gradient and Symmetry). The performance of the proposed noise suppression filter is evaluated by the IMPV(SNR improvement) and the Speckle Index(SI), and the perforamnce of the edge detection is evaluated by the edge detection error rate. According to the evaluated method, The SI reduced about 0.035, The IMPV improved about 1.265(dB), and the edge detection error rate is about 17.5%.

• TTA Journal
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• 통권155호
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• pp.8-15
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• 2014

21세기 정보통신기술의 수많은 화두 중의 하나로 단연 사물인터넷을 꼽는다. 주변의 모든 사물과 사물이 네트워크로 연결되어 궁극적으로 인간의 삶을 보다 유익하고 편리하게 하는 디지털 인프라를 통칭한다. 지난 5월 개최된 서울 디지털포럼의 기조 발표는 아시아 인터넷의 아버지라고 불리는 전길남 교수가 맡았다. 모든 사물의 연결은 TCP/IP 기반의 인터넷이 대표할 것이라는 반증인 셈이다. 미국 와이어드 매거진의 편집국장인 캐빈 캘리는 인터넷을 하나의 글로벌 머신으로 비유하기도 했다. 수년 전 우리나라를 강타했던 3D 영화, 아바타 속 줄거리가 어쩌면 우리의 현실이 될지도 모른다. 세상이 하나로 빠르게 연결되고 있는 것이다. 이에 본 특집에서는 사물인터넷 관련 국내외 기술 표준화 현황 및 전망에 대해 살펴보고자 한다. 먼저 ITU-T 및 JTC 1 등 주요 국제 표준화기구에서의 사물인터넷 표준화 현황과 oneM2M, IEEE, IETF, W3C 등 주요 사실 표준화 기구에서의 사물인터넷 표준화 현황을 정리한다. 또한, GS1에서의 기술 표준화 현황과 국내 사물인터넷포럼에서의 산업체 중심의 표준화 활동에 대해서도 살펴보고자 한다. 모쪼록 금번 사물인터넷 특집을 통해 사물인터넷 분야의 국내 산업체 주도 표준화 노력이 더욱 배가되기를 기대해 본다.

• Korean Journal of Pharmacognosy
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• 제17권2호
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• pp.139-147
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• 1986

Ginseng saponins(GS), protopanaxadiol saponins(PD) and protopanaxatriol saponins (PT) were tested for the inhibition of the development of morphine tolerance and dependence antagonism of morphine analgesia and the loss of morphine tolerance and dependence in mice The results were as follows: 1. Inhibition of the development of morphine tolerance and dependence. 2. Antagonism of morphine analgesia. 3. Increase in the loss of morphine tolerance and dependence. Antagonism of morphine by ginseng saponins and its reversal by L-DOPA and 5-HTP suggest some possibility that catecholamines and serotonin levels might be associated with the results.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.68-73
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• 2004

Ferritin is an iron storage protein found in most living organisms. For expression and industrial use, human light chain ferritin (L-ferritin) was cloned from human liver cDNA library and expressed in Pichia pastoris strain GS115. The recombinant L-ferritin in Pichia pastoris was glycosylated. In a fed-batch culture, the cell mass reached about 57 g/l of dry cell weight, and the L-ferritin in the cell was increased to about 95 mg/l after 150 h. In an atomic absorption spectrometry analysis, the intracellular content of iron in the L-ferritin transformant was measured as $1,694{\pm}85\;\mu\textrm{g}g/g$, which is 5.4-fold more than that of the control strain. This L-ferritin transformant could serve as iron-fortified nutrients in animal feed stock.

• Journal of Microbiology and Biotechnology
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• 제22권5호
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• pp.649-653
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• 2012

A temperate phage was isolated from emetic Bacillus cereus NCTC 11143 by mitomycin C and characterized by transmission electron microscopy and DNA and protein analyses. Whole genome sequencing of Bacillus phage 11143 was performed by GS-FLX. The phage has a dsDNA genome of 39,077 bp and a 35% G+C content. Bioinformatic analysis of the phage genome revealed 49 putative ORFs involved in replication, morphogenesis, DNA packaging, lysogeny, and host lysis. Bacillus phage 11143 could be classified as a member of the Siphoviridae family by morphology and genome structure. Genomic comparisons at the DNA and protein levels revealed homologous genetic modules with patterns and morphogenesis proteins similar to those of other Bacillus phages. Thus, Bacillus phages might have a mosaic genetic relationship.

• Journal of the Korea Safety Management & Science
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• 제17권2호
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• pp.35-45
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• 2015

Total industrial accident rate in Korea is facing a record 0.7% for 10 years since 2000. After entering the continuous versus 0.6% (0.69%) in 2010. In 2011, the decrease was reduced to 0.65%. Conversely In contrast to foreign workers' share has increased dramatically over 10 years since 2000 to 2010 are expected to be difficult in the accident rate of 0.5% for the entry. Despite the ongoing disaster reduction efforts to reduce disaster (share) of foreign workers has been increasing rapidly Survey, Whilst the provision of disaster prevention system status and problem analysis and identify measures that can be established through such disaster reduction measures are urgently needed.

• Journal of Electrical Engineering and Technology
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• 제13권1호
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• pp.220-225
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• 2018

A class E power oscillator is demonstrated for 6.78-MHz wireless power transfer system. The oscillator is designed with a class E power amplifier to use an LC feedback network with a high-Q inductor between the input and the output. Multiple capacitors are used to minimize the variation of the oscillation frequency by capacitance tolerance. The gate and drain bias voltages with opposite characteristics to make the frequency shift of the oscillator are connected in a resistance distribution circuit located at the output of the low drop-out regulator and supplied bias voltages for class E operation. The measured output of the class E power oscillator, realized using the co-simulation, shows 9.2 W transmitted power, 6.98 MHz frequency and 86.5% transmission efficiency at the condition with 20 V $V_{DS}$ and 2.4 V $V_{GS}$.