• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.667-672
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• 2019

We compared two integration systems for stable expression of heterologous genes in Saccharomyces cerevisiae. A Candida glabrata-derived gene was used as the selective marker for the Cre/loxP system, and XYLP, XYLB, GRE3, and XYL2 genes were used as model heterologous genes and ligated into the universal pRS-CMT vector. The resulting pRS-XylP, pRS-XylB, pRS-Gre3, and pRS-Xyl2 plasmids were sequentially integrated into yeast chromosome VII by four integration processes (marker rescue and gene integration). The four introduced genes were successfully expressed. Further, the pRS-PBG2 plasmid harboring expression cassettes for the four genes was constructed for one-step integration. The four genes that were introduced were stably maintained as a gene cluster and were simultaneously expressed. The one-step integration was more effective for the simultaneous integration and expression of the four genes related to xylan/xylose metabolism. This method will enable the generation of a useful biosystem through appropriate use of gene integration methods.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2008.11a
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• pp.127-128
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• 2008

• Journal of Convergence Society for SMB
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• v.4 no.1
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• pp.17-29
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• 2014

The wireless capsule endoscopy played an important role as the evolutionary medical device to solving the difficulties such as diagnosing the intestine diseases. Due to the limited size and functions, it has some drawbacks. The most obstacle thing is the disability of self-motion, it means that it cannot provide the speed problem. Hence, the characteristics of human digestion system is briefly introduced, especially the intestine, to get the information of endoscopy dynamics. Next, in order to make an abstraction of the condition, a new dynamic friction model called LuGre model is introduced and clearly analysed to get the characteristics and the usage of the model. By the consideration of parameters that are tightly related with the real situation of the capsule endoscopy. The Matlab Simulink was applied to build the model and verified by the simulation to discover the features.

• Proceedings of the KSMRM Conference
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• 2001.11a
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• pp.109-109
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• 2001

Purpose： To assess the feasibility of sequential administration of ferumoxides and mangafodi trisodium in the same imaging protocols. Method： Thirty patients underwent double-contrast enhanced MR imaging of liver usi ferumoxides (Fe-MRI) and mangafodipir trisodium (Mn-MRI) on 1.5T GE Horizon system. In twenty patients, Mn-MRI was immediately followed by Fe-MRI. In ten patients, Fe-MR was performed first, then Mn-MRI was performed immediately, In all cases, precontras T1-weighted in-phase and opposed-phase spoiled gradient echo (GRE) images an T2-weighted fast spin-echo images (TR 4000ms, TE 102ms, ETL 8-12) were obtained Fe-MRI was performed with FSE and steady state GRE (TE 10 msec, flip angle 30 sequences. Mn-MRI was performed with in-phase and opposed-phase spoiled GR sequences. The SNR changes after the use of each contrast agents were calculated.

• Investigative Magnetic Resonance Imaging
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• v.23 no.4
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• pp.390-394
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• 2019

Hemosiderosis is characterized by the deposition of excess iron in body tissues. The choroid plexus is an important part of the central nervous system that can be the primary site of iron overload. T2*-weighted gradient echo (GRE) sequence provides high sensitivity for demonstrating cerebral microhemorrhagic foci and iron deposition. In the present study, we describe the case of a 15-year-old boy with acute lymphoblastic leukemia, in whom repeated transfusion led to iron accumulation in the brain. GRE sequence effectively demonstrated hemosiderin deposition in the choroid plexus.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.18 no.1
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• pp.50-58
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• 2009

For precise tracking control of a servo system with nonlinear friction, a robust friction compensation scheme is presented in this paper. The nonlinear friction is difficult to identify the friction parameters exactly through experiments. Friction parameters can be also varied according to contact conditions such as the variation of temperature and lubrication. Thus, in order to overcome these problems and obtain the desired position tracking performance, a robust adaptive back-stepping control scheme with a dual friction observer is developed. In addition, to estimate lumped friction uncertainty due to modeling errors, a DEKF recurrent neural network and adaptive reconstructed error estimator are also developed. The feasibility of the proposed control scheme is verified through the experiment fur a ball-screw system.

• Investigative Magnetic Resonance Imaging
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• v.23 no.4
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• pp.328-340
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• 2019

Purpose: To generate phase images with free of motion-induced artifact and susceptibility-induced distortion using 3D radial ultrashort TE (UTE) MRI. Materials and Methods: The field map was theoretically derived by solving Laplace's equation with appropriate boundary conditions, and used to simulate the image distortion in conventional spin-warp MRI. Manufacturer's 3D radial imaging sequence was modified to acquire maximum number of radial spokes in a given time, by removing the spoiler gradient and sampling during both rampup and rampdown gradient. Spoke direction randomly jumps so that a readout gradient acts as a spoiling gradient for the previous spoke. The custom raw data was reconstructed using a homemade image reconstruction software, which is programmed using Python language. The method was applied to a phantom and in-vivo human brain and abdomen. The performance of UTE was compared with 3D GRE for phase mapping. Local phase mapping was compared with T₂* mapping using UTE. Results: The phase map using UTE mimics true field-map, which was theoretically calculated, while that using 3D GRE revealed both motion-induced artifact and geometric distortion. Motion-free imaging is particularly crucial for application of phase mapping for abdomen MRI, which typically requires multiple breathold acquisitions. The air pockets, which are caught within the digestive pathway, induce spatially varying and large background field. T₂* map, that was calculated using UTE data, suffers from non-uniform T₂* value due to this background field, while does not appear in the local phase map of UTE data. Conclusion: Phase map generated using UTE mimicked the true field map even when non-zero susceptibility objects were present. Phase map generated by 3D GRE did not accurately mimic the true field map when non-zero susceptibility objects were present due to the significant field distortion as theoretically calculated. Nonetheless, UTE allows for phase maps to be free of susceptibility-induced distortion without the use of any post-processing protocols.

• 제어로봇시스템학회:학술대회논문집
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• 1992.10b
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• pp.219-224
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• 1992

This paper deals with the linear quadratic optimal regulator problem for descriptor systems without performing a preliminary transformation for a descriptor system. We derive a generalized Riccati differential equation (GRDE) based on the two-point boundary value problem for a Hamiltonian equation. We then obtain an optimal feedback control and the optimal cost in terms of the solution of GRE. A simple example is included.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.43 no.4
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• pp.648-657
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• 1994

This paper presents a solution of the H$\infty$ control problem for a linear singularly perturbed system. A sufficient condition for a linear singularly perturbed system to achieve the prescribed disturbance attenuation level is obtained. Based upon this sufficient condition, an H$\infty$ controller design method which involves the solutions of two generalized algebraic Riccati equations(GRE) is developed.

• Proceedings of the Korean Society of Computer Information Conference
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• 2014.01a
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• pp.467-468
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• 2014

본 논문에서는 파일 내용 기반 검색 프로그램을 설계하고 구현하였다. 역 인덱스 구조를 이용하여 설계하였으며 별도의 정보 검색 라이브러리 사용 없이 구현하였다. 인덱스 파일은 트라이 자료 구조를 직접 설계 및 구현 하였으며 자바 언어의 HashMap 구조를 중첩 형태로 구현하였다. 개발 시스템의 유용성을 테스트하기 위해 GRE 단어집에 수록된 약 3,300개의 단어를 사용하여 임의 생성한 텍스트 파일 집합을 사용하였다.