• Korean Journal of Pharmacognosy
• /
• v.31 no.1
• /
• pp.7-15
• /
• 2000

Glycyrrhizae Radix aqua-acupuncture solution (GRAS) and Glycyrrhizae Radix water-extracted solution (GRWS) were prepared and tested for organ toxicities, antitumor activities, and immunomodulatory effects. The organ-toxicity of GRAS to male ICR mice was studied by the measurements of glutamic oxaloacetic transaminase (GOT), glutamic pyruvate transaminase (GPT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP-s) activities after injection of GRAS for 7 days. The activities of GOT, GPT, LDH, ALP-s were decreased with GRAS. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of GRAS at 1.5g/ml and 3g/ml resulted in more than 80% inhibition of growth in Ehrlich ascites tumor cells (EATC), Hepa1c1c7, and HeLa cells. Toxicity of GRAS to A549 revealed that 68% inhibition of growth. GRWS at the concentration of 3g/ml showed more than 80% inhibition of growth with EATC, Hepalclc7, A549 and HeLa. In morphological study, the number of cells were decreased, and the shape of cells was round-form in EATC, Hepalclc7, A549 and HeLa cells with GRAS. Administration of GRAS inhibited the growth of EATC in vivo. Mice given EATC at 1.5g/ml or 0.3g/ml GRAS had 16.7% to 50% survival after 21 days. GRAS increased the proliferation of T and B cells and the cytolytic activity of purified T cell. The biosyntheses of nucleic acid and protein of EATC, Hepalclc7, A549 and HeLa cells were inhibited by GRAS.

• Proceedings of the Korean Information Science Society Conference
• /
• 1999.10b
• /
• pp.635-637
• /
• 1999

GRASS는 GIS 공개 소프트웨어로서, 이의 효과적인 활용은 비용 면이나 기술 개발 및 축적 면에서 국가적으로 중요하다. 생물, 지질, 환경 등의 과학 기술 분야에서는 방대한 자료를 3차원적으로 표현하고 분석해야만 하는 경우가 많이 있다. 본 논문은 GRASS의 3차원 기능을 이용하여 식물의 서식 분포를 시각적으로 표현하는 방법과 실제 구현 사례를 기술한다. 지역으로는 내장산을 선택하였으며, 환경부에서 제공하는 실제 내장산 DEM 자료를 사용하였다. 식물 분포 표시를 위해 GRASS가 제공하는 컬러-기반 3차원 디스플레이 기능을 사용하였다.

• The Microorganisms and Industry
• /
• v.27 no.1
• /
• pp.18-22
• /
• 2001

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.11a
• /
• pp.380-380
• /
• 2005

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.5 no.1
• /
• pp.84-89
• /
• 1985

Chemical composition, dry matter and cellulose digestibilities and fermentation rates of alfalfa and orchard grass cut at different time were estimated in vitro method and the results summurized as follows: 1. Crude protein, crude fiber cwc and cellulose content of Alfalfa were decreassed as advancing cutting time. Crude protein, crude fiber and cwc content of Orchard grass were decreased as advancing cutting time up to 3 cutting, but crude protein was slightly increased at 4th cutting, crude fiber and cellulose content were higher at 2nd cutting, but decreased thereafter. 2. DM digestibility of Alfalfa was 51.80, 51.86, 52.92 and 59.52% at 1, 2, 3 and 4th cutting time, respectibly, thus slightly increased as advancing cutting time, and cellulose digestibility of Alfalfa was not much different with different cutting time. DM digestibility of Orchard grass was 62.21, 66.10, 60.95 and 66.32% at 1, 2, 3 and 4th cutting time, respectibly, and cellulose digestibility of Orchard grass was slightly increased at 3rd cutting time and then increased at 4th cutting time. 3. Fermentation rate of DM of alfalfa was the highest 1st cutting time (0.83%/hr.) and was not different at 3 and 4th cutting time. Cellulose fermentation rate of Alfalfa was the highest at 1st cutting time (1.29%/hr.), decreased at 2 and 3th and then increased at 4th cutting time. Fermentation rate of DM of Orchard grass was 1.42, 1.58, 1.60 and 1.57%/hr. and of cellulose was the highest at 2nd cutting time (1.77%/hr.)

• Journal of Microbiology and Biotechnology
• /
• v.16 no.12
• /
• pp.2000-2003
• /
• 2006

A gene (GenBank AF096282) coding for a $\alpha$-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable $\alpha$-glucosidase was produced inside of the GRAS host at 0.04 unit/mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/mg-dry cell by the inductively expressing GALl0 promoter, respectively. No $\alpha$-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or $\alpha$-amylase signal sequence from Aspergillus oryzae were fused before the $\alpha$-glucosidase gene for the secretion.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.5 no.1
• /
• pp.33-36
• /
• 1985

This experiment was carried out to investigate the effect of crown density of trees on the growth and yield of cool-season grasses in forest. The species used in this study was orchard-grass (Dactylis glomerata L.) and 4 levels of tree crown density(O=full sunlight, 25, 50 and 75%) were treated. The experiment was performed at the experimental filed of the Livestock Experiment Station in Suweon, during 1979 to 1950. The results obtained are summarized as follows: 1. Maximum leaf area was obtained at 25% crown density of trees, followed by 0, 50 and 75%, regardless of cutting times. 2. Plant height tended to increase as the crown density of trees increased. However, there was no difference between 0% and 25% crown density of trees. 3. There was a negative correlation between plant height and leaf area of orchardgrass grown under pine trees. 4. The more dry matter yield of orchardgrass was obtained at 25% crown density of trees (p<0.05), follwed by 0, 50 and 75%, respectively. However, there was no significant difference between 0% and 50% crown density of trees. Therefore it is suggested that the critical level of crown density of trees is 50% to culture of cool-season grasses in forest.

• Korean Journal of Soil Science and Fertilizer
• /
• v.40 no.3
• /
• pp.173-180
• /
• 2007

Reclaimed tidal area is a great agricultural resource in the world. Improvement and utilization of reclaimed soil is an important measure for expanding land resource. This study was conducted to evaluate relative salt-tolerance of plants and its effect for improving saline soil. Eighteen tolerance plants were selected from China, Pakistan and Korea the climate of which is different. The emergence of different varieties in reclaimed soil was in order as FL478>Barnyard grass>Pokkali>Atriplex>Sesbania>Rumex>Alfalfa>Tall Fescue>Ryegrass>Sudan grass. Four varieties, Barnyard grass, Sesbania, Atriplex and Limonium were selected for soil improvement in reclaimed land. Cultivation of Sesbania, Barnyard grass and Atriplex were good to soil physico-chemical quality. Also these plants increased soil organic matter contents and reduced soil salt concentration. Organic matter contents of cultivated soils of Sesbania, Barnyard grass and Atriplex were $4.10g\;kg^{-1}$, $4.60g\;kg^{-1}$ and $2.81g\;kg^{-1}$ respectively. On the other hand organic matter content of uncultivated soils was $2.65g\;kg^{-1}$. As Sesbania and Barnyard grass were applied to cultivated soil like green manure, bulk density improved from $1.42Mg\;m^{-3}$ to $1.39Mg\;m^{-3}$.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.31 no.3
• /
• pp.217-222
• /
• 2011

Success in molecular breeding for better adapted varieties to environmental stresses depend upon the concerted efforts by various research including tissue culture, transformation, genetics and breeding. In order to optimize tissue culture conditions of Siberian wildrye grass, the effects of plant growth regulators on callus induction and plant regeneration were investigated with mature seeds. The highest callus induction frequency was observed when the mature seeds were cultured on MS medium supplemented with 5 mg/L 2,4-D. The highest plant regeneration frequency was observed when callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of Siberian wildrye grass by the production of transgenic plant.

• 건강소식
• /
• v.36 no.5
• /
• pp.16-19
• /
• 2012

얼마 전 워싱턴포스트지에 카오르의 전통음식 푸아그라(Foie Gras)와 코코뱅이 소개된 적이 있었다. 푸아그라는 거위의 간을 이용하여 만든 요리이고 코코뱅은 닭고기와 야채에 레드와인을 넣어 요리한 음식이다. 신문기사에 의하면 카오르 사람들은 푸아그라와 코코뱅과 같은 기름기가 많은 동물성 식품을 많이 먹는 데도 오래 산다는 것이었다. 나는 이를 확인하기 위해 프랑스 남부에 있는 마을 카오르를 찾았다.