• Molecules and Cells
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• 제24권2호
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• pp.294-300
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• 2007

The mammalian glycosylphosphatidylinositol (GPI) anchor consists of three mannoses attached to acylated GlcN-(acyl)PI to form $Man_3$-GlcN-(acyl)PI. The first of the three mannose groups is attached to an intermediate to generate Man-GlcN-(acyl)PI by the first mannosyltransferase (GPI-MT-I). Mammalian and protozoan GPI-MT-I have different substrate specificities. PIG-M encodes the mammalial GPI-MT-I which has 423 amino acids and multiple transmembrane domains. In this work we cloned PIG-M homologues from humans, Plasmodium falciparum (PfPIG-M), and Saccharomyces cerevisiae (GPI14), to test whether they could complement GPI-MT-I-deficient mammalian cells, since this biosynthetic step is likely to be a good target for selective screening of inhibitors against many pathogenic organisms. PfPIG-M partially restored cell surface expression of the GPI-anchored protein CD59 in PIG-M deficient mammalian cells, and first mannose transfer activity in vitro; however, this was not the case for GPI14.

• 운동영양학회지
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• 제23권2호
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• pp.28-33
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• 2019

[Purpose] Recent studies have shown that glucose-6-phosphate isomerase (GPI)-which is a glycolysis interconversion enzyme-reduces oxidative stress. However, these studies are limited to tumors such as fibrosarcoma, and there are no studies that have examined the effects of exercise on GPI expression in mice skeletal muscle. Furthermore, GPI acts in an autocrine manner thorough its receptor, autocrine motility factor receptor (AMFR); therefore, we investigated expression level changes of secreted GPI from skeletal muscle in in vitro study to examine the potential role of GPI on skeletal muscle. [Methods] First, we performed an in vitro study, to identify the condition that upregulates GPI levels in skeletal muscle cells; we treated C2C12 muscle cells with an exercise-mimicking chemical, AICAR. AICAR treatment upregulated GPI expression level in C2C12 cell and its secretomes. To confirm the direct effect of GPI on skeletal muscle cells, we treated C2C12 cells with GPI recombinant protein. [Results] We found that GPI improved the viability of C2C12 cells. In the in vivo study, the exercise-treated mice group showed upregulated GPI expression in skeletal muscle. Based on the in vitro study results, we speculated that expression level of GPI in skeletal muscle might be associated with muscle function. We analyzed the association between GPI expression level and the grip strength of the all mice group. The mice group's grip strengths were upregulated after 2 weeks of treadmill exercise, and GPI expression level positively correlated with the grip strength. [Conclusion] These results suggested that the exercise-induced GPI expression in skeletal muscle might have a positive effect on skeletal muscle function.

• Archives of Pharmacal Research
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• 제22권3호
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• pp.249-254
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• 1999

Enzymatic conversion of brain glycosylphosphatidylinositol-linked alkaline phosphatase (GPI-AP), amphiphilic, was examined. When GPI-AP was incubated with glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD), a negligible conversion of GPI-AP to hydrophilic form was observed. The inclusion of monoacylglycerols enhanced the enzymatic conversion, although the action of monoacylglycerols differed greatly according to the size of acyl group; the enzymatic conversion was enhanced considerably in the presence of monoacylglycerols possessing acyl group of longer chain length ($C_{10-}C_{18}$), which monoacylglycerols with acyl moiety of shorter length ($C_{4-}C_{8}$) did fail to augment the enzymatic conversion. Noteworthy, monooleoylglycerol was much more effective than the other monoacylglycerols in promoting the enzymatic conversion, indicating a beneficial role of the unsaturation in acyl chain. Meanwhile, ionic amphiphiles such as monohexadecyllysophosphatidylcholoine and palmitoyl-carnitine decreased the enzymatic conversion of GPI-AP in a concentration-dependent manner, with monohexadecyllysophosphatidylcholine and palmitoyl-carnitine deceased the enzymatic conversion of GPI-AP in a concentration-dependent manner, with monohexadecyllysophosphatidylcholoine being more inhibitory than palmitoylcarnitine. Separately when GPI-AP was exposed to various oxidants prior to the incubation with GPI-PLD, a remarkable decrease of the enzymatic conversion was observed with hypochlorite and peroynitrite generators, but not $H_{2}O_{2}$. In further study, hypochlorite was found to inactivate GPI-PLD at low concentrations ($3~100{\mu}M$). From these results, it is suggested that the enzymatic conversion of GPI-AP by GPI-PLD may be regulated in vivo system.

• 한국지구과학회지
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• 제33권7호
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• pp.583-595
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• 2012

대기 및 해양의 대규모 환경에서 열대저기압 발생의 잠재적 빈도는 잠재생성지수(GPI; Genesis Potential Index)를 이용하여 예측할 수 있다. 본 연구에서는 18개의 CMIP5 기후모델을 이용하여 GPI의 연진동 및 경년변동성이 분석되었다. 비교를 위하여 재분석자료로부터 계산된 GPI의 연진동이 재조명되었다. 특히 CMIP5 기후모델과 재분석자료에 의한 GPI가 비교되었고, 그 차이에 대한 가능한 해석이 논의되었다. ENSO (El Nino and Southern Oscillation)는 열대 저기압 발생 및 경로에 영향을 주는 열대 기후현상이다. 잠재생성지수가 네 개의 대규모 매개변수의 함수임을 이용함으로써 열대저기압발생에 대한 역학적 해석이 제시되었다. 본 연구에서는 엘니뇨 혹은 라니냐 해에 GPI 편차를 논의하였고, 그 편차에 가장 영향을 많이 주는 인자를 찾았다. 또한 여러 대규모 인자를 활용하여 북태평양지역 열대저기압 발생에 대하여 가능한 기작을 논의하였다.

• Journal of Microbiology
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• 제43권3호
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• pp.266-271
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• 2005

In eukaryotic cells, various proteins are anchored to the plasma membrane through glycosylphosphatidylinositol (GPI). To study the biosynthetic pathways and modifications of GPI, various mutant cells have been isolated from the cells of Chinese hamster ovaries (CHO) supplemented with several exogenous genes involved in GPI biosynthesis using aerolysin, a toxin secreted from gram-negative bacterium Aeromonas hydrophila. Alpha toxin from Gram-positive bacterium Clostridium septicum is homologous to large lobes (LL) of aerolysin, binds GPI-anchored proteins and possesses a cell-destroying mechanism similar to aerolysin. Here, to determine whether alpha toxins can be used as an isolation tool of GPI-mutants, like aerolysin, CHO cells stably transfected with several exogenous genes involved in GPI biosynthesis were chemically mutagenized and cultured in a medium containing alpha toxins. We isolated six mutants highly resistant to alpha toxins and deficient in GPI biosynthesis. By genetic complementation, we determined that one mutant cell was defective of the second subunit of dolichol phosphate mannose synthase (DPM2) and other five cells were of a putative catalytic subunit of inositol acyltransferase (PIG-W). Therefore, C. septicum alpha toxins are a useful screening probe for the isolation of various GPI-mutant cells.

• Parasites, Hosts and Diseases
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• 제47권3호
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• pp.197-204
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• 2009

Trypanosoma brucei, a protozoan parasite, causes sleeping sickness in humans and Nagana disease in domestic animals in central Africa. The trypanosome surface is extensively covered by glycosylphosphatidylinositol (GPI)-anchored proteins known as variant surface glycoproteins and procyclins. GPI anchoring is suggested to be important for trypanosome survival and establishment of infection. Trypanosomes are not only pathogenically important, but also constitute a useful model for elucidating the GPI biosynthesis pathway. This review focuses on the trypanosome GPI biosynthesis pathway. Studies on GPI that will be described indicate the potential for the design of drugs that specifically inhibit trypanosome GPI biosynthesis.

• 전기학회논문지
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• 제65권8호
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• pp.1383-1391
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• 2016

This paper is concerned with applicability of a new type of controllers, called i-PID and GPI in which unknown parts of the plant are taken into account without any modeling procedure, to automatic voltage regulator (AVR) system. First, the procedure for applying i-PID and GPI algorithms to AVR system is proposed, which uses model reduction technique based on the given information of AVR. Second, simulations are given to verify their effectiveness comparing to various PID algorithms including PIDD2 which is four-term controller, that is, consisting of PID and second order derivative terms. Superior response performances of i-PID and GPI in comparison to conventional PID controllers are shown. Moreover, i-PID can highly improve the system robustness with respect to model uncertainties, especially to load variations.

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.197.1-197.1
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• 2000

• Spatial Information Research
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• 제22권4호
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• pp.49-58
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• 2014

최근 전 세계적으로 지구온난화에 따른 기후변화로 태풍, 폭염, 폭설등과 같은 자연재해의 피해가 대규모로 확대되고 있다. 근본적으로 지구온난화를 유발하는 가장 큰 원인은 대기 중의 온실가스를 들 수 있으며, 온실가스의 농도 증가로 인해 우리나라가 속해있는 북반구는 점점 더 지구표면온도가 증가하고 있고, 그에 따른 극한 기상 발생률이 크게 증가하고 있다. 본 연구에서는 최근 이산화탄소 농도 추세를 반영한 RCP(Representative Concentration Pathway) 8.5 시나리오를 이용하여 미래의 태풍발생의 공간분포를 추정하였다. 공간분포를 추정하기 위해 먼저 RCP 8.5 월 자료를 사용하여 1982~2100년 기간 동안의 태풍발생지수(GPI; Genesis Potential Index)를 계산하였다. 1982~2010년 동안 발생한 태풍의 발생위치정보와 월평균 GPI 값을 이용하여 태풍발생의 확률분포(PDF)를 추정하였으며, PDF의 0.05, 0.1 및 0.15에 해당하는 GPI의 범위를 설정하여 0.05GPI, 0.1GPI 및 0.15GPI로 정의하였다. 이를 바탕으로 1982~2010년, 2011~2040년, 2041~2070년, 2071~2100년의 태풍발생의 공간 확률 분포를 추정 하였으며, 공간 확률 분포와 함께 과거 태풍발생정보를 이용하여 공간밀도를 분석하였다. 분석 결과, 미래에 태풍이 발생할 가능성이 높은 지역이 필리핀의 동쪽에 위치한 위도 $10^{\circ}{\sim}20^{\circ}$ 영역으로 나타났다. 이러한 결과를 통해 추후 미래의 태풍발생 가능지역을 추정하고, 이를 기반으로 태풍의 경로를 추정하는데 활용하여 태풍의 발생 위치에 따라 한반도에 미치는 영향을 추정하는데 활용할 수 있을 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제25권7호
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• pp.1055-1060
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• 2004

A new inhibitor for peptidylprolyl cis-trans isomerase (PPIase) has been isolated from Ganoderma lucidum and purified to homogeneous state by organic solvent extraction. The purified PPIase inhibitor (GPI) is assumed to be a membrane-associated glycoprotein. GPI inhibits specifically the bovine brain PPIase, a cyclophilin, and has no effect on the FKBP activity. The results of our chemical modification study of GPI indicate the presence of Lys residue(s) at or near its binding site. Like CsA-cyclophilin complex, GPI-bovine brain PPIase complex strongly inhibits the calcineurin activity in vitro, suggesting the possible involvement of GPI in immunomodulating pathway by the formation of PPIase-inhibitor-calcineurin complex.