• The Plant Pathology Journal
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• v.26 no.1
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• pp.80-82
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• 2010

A stem spot disease was observed on Gymnocalycium mihanovichii (Korean name: Bimoran), a scion of graftcactus, in major growing areas of Goyang and Eumseong, Korea during 2008 and 2009. Typical symptoms were initial blackish brown lesions produced mainly on areoles and scars of injured stem tissues, gradually becoming large black spots. A causal organism isolate CD2-7A isolated from the infected stems was identified as Alternaria alternata based on its morphological characteristics and confirmed by the DNA sequencing analysis of ITS, glyceraldehydes-3-phosphate dehydrogenase (gpd), and allergen Alt a1 (Alt a 1) genes. Artificial inoculation of the fungal isolate CD2-7A produced the same rot symptoms on the cactus stems, from which the same fungus was isolated and identified. This is the first report of the black spot caused by A. alternata in the grafted cactus.

• Ocean and Polar Research
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• v.43 no.1
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• pp.1-14
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• 2021

In this study, extreme wind speeds in the Western North Pacific (WNP) were estimated using reanalysis wind fields synthesized with an empirical typhoon vortex model. Reanalysis wind data used is the Fifth-generation European Centre for Medium-Range Weather Forecasts (ECMWF) reanalysis (ERA5) data, which was deemed to be the most suitable for extreme value analysis in this study. The empirical typhoon vortex model used has the advantage of being able to realistically reproduce the asymmetric winds of a typhoon by using the gale/storm-forced wind radii information in the 4 quadrants of a typhoon. Using a total of 39 years of the synthesized reanalysis wind fields in the WNP, extreme value analysis is applied to the General Pareto Distribution (GPD) model based on the Peak-Over-Threshold (POT) method, which can be used effectively in case of insufficient data. The results showed that the extreme analysis using the synthesized wind data significantly improved the tendency to underestimate the extreme wind speeds compared to using only reanalysis wind data. Considering the difficulty of obtaining long-term observational wind data at sea, the result of the synthesized wind field and extreme value analysis developed in this study can be used as basic data for the design of offshore structures.

• The Korean Journal of Applied Statistics
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• v.23 no.4
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• pp.651-668
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• 2010

Various estimators of two risk measures of a specific financial portfolio, Value-at-Risk and Expected Shortfall, are compared for each case of 1-day and 10-day horizons. We use the Korea Composite Stock Price Index data of 20-year period including the year 2008 of the global financial crisis. Indexes of five foreign stock markets are also used for the empirical comparison study. The estimator considering both the heavy tail of loss distribution and the conditional heteroscedasticity of time series is of main concern, while other standard and new estimators are considered too. We investigate which estimator is best for the Korean stock market and which one shows the best overall performance.

• Mycobiology
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• v.38 no.4
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• pp.331-335
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• 2010

In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using the partial ftx gene (410 bp) along with the hygromycin B phosphotransferase gene (hygB) downstream of the glyceraldehydes-3-phosphate dehydrogenase (gpd) promoter. The plasmid was transformed into protoplasts of monokaryotic strain 4019-20 of F. velutipes by electroporation. High transformation efficiency was obtained with an electric-pulse of 1.25 kV/cm by using 177 transformants/${\mu}g$ of DNA in $1{\times}10^7$ protoplasts. PCR and Southern blot hybridization indicated that a single copy of the plasmid DNA was inserted at different locations in the F. velutipes genome by non-homologous recombination. Therefore, this transformation system could be used as a useful tool for gene function analysis of F. velutipes.

• The Plant Pathology Journal
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• v.31 no.1
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• pp.78-82
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• 2015

In 2011, a leaf blight disease was observed on cosmos (Cosmos bipinnatus) leaves in Nonsan, Korea. The causal pathogen was isolated and identified based on morphological and molecular approaches. Morphological characteristics of the pathogen matched well with the Alternaria cosmosa and also easily distinguishable from Alternaria zinniae reported from cosmos seeds by producing branched beak. Phylogenetically, the pathogen could not be distinguished from A. passiflorae based on the sequence analysis of a combined data set of Alt a1 and gpd genes. However, A. passiflorae was distinguished from the present species by having conidiophores with 4 to 5 conidiogenous loci. The results indicate that the present Alternaria species is A. cosmosa. Pathogenicity tests revealed that the isolate was pathogenic to the leaves of Cosmos bipinnatus. This is the first report of Alternaria blight disease caused by A. cosmosa on cosmos in Korea.

• Mycobiology
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• v.43 no.4
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• pp.384-391
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• 2015

In the course of survey of endophytic fungi from Bangladesh pumpkin seeds in 2011~2012, two strains (CNU111042 and CNU111043) with similar colony characteristics were isolated and characterized by their morphology and by molecular phylogenetic analysis of the internal transcribed spacer, glyceraldehydes-3-phosphate dehydrogenase (gpd), and Alternaria allergen a1 (Alt a1) sequences. Phylogenetic analysis of all three sequences and their combined dataset revealed that the fungus formed a subclade within the A. alternata clade, matching A. burnsii and showing differences with its other closely related Alternaria species, such as A. longipes, A. tomato, and A. tomaticola. Long ellipsoid, obclavate or ovoid beakless conidia, shorter and thinner conidial size ($16{\sim}60[90]{\times}6.5{\sim}14[{\sim}16]{\mu}m$) distinguish this fungus from other related species. These isolates showed more transverse septation (2~11) and less longitudinal septation (0~3) than did other related species. Moreover, the isolate did not produce any diffusible pigment on media. Therefore, our results reveal that the newly recorded fungus from a new host, Cucurbita maxima, is Alternaria burnsii Uppal, Patel & Kamat.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.287-292
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• 2000

Recombinant Aspergillus niger was constructed to express and secrete a biologically active murine granulaocyte macrophage-colony stimulating factor (mGM-CSF). A 500 bp fragment encoding the signal peptide and terminator of glyceraldehyde-3-phosphate dehydrogenase (gpd). The hygromycin phosphotrasferase gene (hph) was used as a selection marker for the fungal transformants. An expression vector was introduced into A. niger ATCC 9642, and a Northern blot analysis indicated the presence of a considerable amount of transcripts from the introduced mGM-CSF. The biological activity of recombinant mGM-CSF (rmGM-CSF) isolated from the culture filtrate was confirmend by measuring the proliferationof the GM-CSF dependent FDC-P1 cell line. It appeared that rmGM-CSF was amenable to the proteolytic activity produced by A. niger, since biological actibity was only observed when the transformants were grown in a protease-repressing medium, and the activity of rmGM-CSF dramatically decreased with an increase of age of the culture. The yield of rmGM-CSF, as determined by ELISA. was 640 ng/l of culture filtrate. Accordingly, its specific activity is estimated to be approximately two-and-a-half times higher than that of a commercial preparation from E. coli.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.1
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• pp.179-184
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• 2004

This paper presents an image segmentation algorithm to obtain the 3D body shape data that the grid pattern and the body contour lute in the background image are extracted using the new proposed hybrid method. The body contour line is extracted based on maximum biased anisotropic recognition(MaxBAR) algorithm which recognizes the most strong and robust edges in the image since the normal derivative at the edges is large, while the tangential derivatives can be small. The grid patterns within body contour lines are extracted by grid pattern detection (GPD). The body contour lilies and the grid patterns are combined. The consecutive run test based on heuristic method is used to link the disconnected line and reduce noise line. This proposed segmentation method is more effective than the conventional method which uses a gradient and a laplacian operator, verified with application two conventional method.

• Animal cells and systems
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• v.1 no.4
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• pp.529-534
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• 1997

Genetic and morphological variations of Rhinogobius giurinus were surveyed. Populations of R.giurinus were clearly divided into two forms (Form-A and Form-B). Starch gel variation of this species. Three loci (Aco, Mpi, aGpd) out of 27 showed fixed allelic differences between Form-A and Form-B and they are well differentiated from each other genetically (Rogers' S=0.871). These two forms, moreover, are found to be allopatric in distribution and morphologi cally different in body length and caudal fin color pattern. Therefore, they are considered as two distinct taxa of subspecific rank. In addition, the genetic relationships among 5 taxa within 4 species of the genus Rhinogobius were investigated. Three species of the Rhinogobius brunneus complex (R.sp. OR, R. sp. CB and R. sp. CO) are well differentiated from each other genetically and two taxa of R. giurinus are genetically divergent from three species of the Rhinosotius brunneus complex (average Nei's D=0.603, average Rogers'=0.534).

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.200-200
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• 2022

본 연구에서는 총 강우량과 강우강도을 고려한 이변수 분석으로 연최대 호우사상을 선별하고, 두 변수를 Copula 함수로 결합하여 최적의 모델조합을 찾는 확률호우사상 산정 방법론을 제시하였다. 국내 69개 관측소의 2020년까지의 관측 자료를 대상으로 1mm 이하의 강우는 제거한 뒤, IETD(Inter-Event Time Definition) 12시간을 기준으로 강우자료를 독립적인 호우사상으로 분리하였다. 호우사상의 여러 특성 중 양의 상관관계를 갖는 총 강우량과 강우강도를 변수로 선택해 이변수 지수분포에 대입하였고, 각 지점의 연최대 호우사상 시계열을 생성하였다. 2변수 지수분포의 매개변수는 전체 기간과 연도별로 나누어 추정해 본 결과 연도별 변동성이 큰 것을 확인해 연도별 추정 방식을 선택하였다. 연최대 강우사상 시계열의 총 강우량과 강우강도는 극한 강우에 적용하는 확률분포형 중 Lognarmal, Gamma, Gumbel, GEV(Generalized Extreme Value), GPD(Generalized Pareto Distribution) 5가지를 사용하여 각각 CDF(Cumulative distribution Function) 값을 추정하였다. 계산된 CDF 값은 3가지 Copula 모형으로 결합해 joint CDF 값을 산출하였다. 총 75개의 모델조합 중 최적 모델을 찾기 위해 CVM(Cramer-von-Mises) 적합도 검정을 시행하였다. CVM의 통계량 Sn 값이 가장 작은 모델조합을 해당 지점의 최적 모델조합으로 선정하였다.