The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

First Report of Black Spot Caused by Alternaria alternata on Grafted Cactus

  • Choi, Min-Ok (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Sang-Gyu (Department of Agricultural Biotechnology, Seoul National University) ;
  • Hyun, Ik-Hwa (National Plant Quarantine Service) ;
  • Kim, Jeong-Ho (Korea Turfgrass Institute, Subsidiary of Korea Golf Course Business Association) ;
  • Cho, Chang-Hui (Cactus Research Institute, Gyeonggi-do Agricultural Experimental Station) ;
  • Park, Myung-Soo (Chemical Biotechnology Research Center, KRICT) ;
  • Kim, Young-Ho (Department of Agricultural Biotechnology, Seoul National University)
  • Received : 2009.06.25
  • Accepted : 2009.11.25
  • Published : 2010.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

A stem spot disease was observed on Gymnocalycium mihanovichii (Korean name: Bimoran), a scion of graftcactus, in major growing areas of Goyang and Eumseong, Korea during 2008 and 2009. Typical symptoms were initial blackish brown lesions produced mainly on areoles and scars of injured stem tissues, gradually becoming large black spots. A causal organism isolate CD2-7A isolated from the infected stems was identified as Alternaria alternata based on its morphological characteristics and confirmed by the DNA sequencing analysis of ITS, glyceraldehydes-3-phosphate dehydrogenase (gpd), and allergen Alt a1 (Alt a 1) genes. Artificial inoculation of the fungal isolate CD2-7A produced the same rot symptoms on the cactus stems, from which the same fungus was isolated and identified. This is the first report of the black spot caused by A. alternata in the grafted cactus.

Keywords

References

  1. Berbee, M. L., Pirseyedi, M. and Hubbard, S. 1999. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehydes-3-phosphate dehydrogenase gene sequences. Mycologia 91:964-977. https://doi.org/10.2307/3761627
  2. Chang M., Hyun, I. H. and Lee, Y. H. 1988. Bipolaris stem rot of cactus caused by Bipolaris cactivora (Petrak) Alcorn. Korean J. Plant Pathol. 14:661-663.
  3. Ellis, M. B. 1977. Dematiaceous Hyphomycetes. Common wealth Mycological Institute. Kew, Surrey, England. 608 pp.
  4. Hong, S. G., Cramer, R. A., Lawrence, C. G. and Pryor, B. M. 2005. Alt a 1 allergen homologs from Alternaria and related taxa: analysis of phylogenetic content and secondary structure. Fungal Genet. Biol. 42:119-129. https://doi.org/10.1016/j.fgb.2004.10.009
  5. Hyun, I. H., Lee, S. D., Lee, Y. H. and Heo, N. Y. 1988. Mycological characteristics and pathogenicity of Fusarium oxysporum Schlecht. Emend. Snyd. & Hans. causing stem rot of cactus. Korean J. Plant Pathol. 14:463-466.
  6. Jeong, I. H., Kim, G. H., Lim, M. T., Hur, J.-S., Shin, J. S. and Koh, Y. J. 2008. Brown ring spot on leaves of kiwifruit caused by Alternaria alternata. Res. Plant Dis. 14:68-70. https://doi.org/10.5423/RPD.2008.14.1.068
  7. Kim, J. H., Jeoung, M.-I., Hyun, I.-H. and Kim, Y. H. 2004. Potential biotypes in Korean isolates of Bipolaris cactivora associated with stem rot of cactus. Plant Pathol. J. 20:165-171. https://doi.org/10.5423/PPJ.2004.20.3.165
  8. Kim, Y. H., Jun, O. K., Sung, M. J., Shin, J.-S., Kim, J. H. and Jeoung M.-I. 2000. Occurrence of Colletotrichum stem rot caused by Glomerella cingulata on graft-cactus in Korea. Plant Pathol. J. 16:242-245.
  9. Kwon, J.- H., Ahn, G.-H. and Park, C.-S. 2004. Black spot of sweet persimmon (Diospyros kaki) caused by Alternaria alternata. Res. Plant Dis. 10:183-187. https://doi.org/10.5423/RPD.2004.10.3.183
  10. Park, K. S. and Lee, S. G. 2003. Leaf spot of safflower (Carthamus thinctorius) caused by Alternaria carthami and A. alternata. Res. Plant Dis. 9:159-161. https://doi.org/10.5423/RPD.2003.9.3.159
  11. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Application eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and J. J. White, pp. 315-322. Academic Press, New York, USA.
  12. Yu, S. H. 2001. Korean Species of Alternaria and Stemphylium. National Institute of Agricultural Science and Technology, Suwon, Korea 212 pp.

Cited by

  1. Investigation of disease occurrences and rapid diagnosis in grafted cactus 2017, https://doi.org/10.1007/s41348-017-0093-9
  2. Gray Mold of Grafted Cactus Caused by Botrytis cinerea in Korea vol.28, pp.2, 2012, https://doi.org/10.5423/PPJ.2012.28.2.218
  3. Biocontrol Characteristics of Bacillus Species in Suppressing Stem Rot of Grafted Cactus Caused by Bipolaris cactivora vol.29, pp.1, 2013, https://doi.org/10.5423/PPJ.OA.07.2012.0116
  4. Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti vol.32, pp.1, 2016, https://doi.org/10.5423/PPJ.NT.09.2015.0184