• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.246-246
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• 2016

본 연구에서는 최근 기후변화로 인한 집중호우의 발생횟수의 경향을 확률적으로 분석함에 있어 1개월 동안 80 mm/day 이상의 강우사상을 집중호우로 정의하여, 대구 및 부산 강우관측소로부터 수집된 384개월 동안의 집중호우를 분석하였다. 집중호우 월별 발생횟수와 같은 형식의 자료의 확률적 분석은 대개 Poisson 분포 (POI)가 사용되나 자료에 포함된 0자료의 과잉은 확률분포를 왜곡시키는 문제를 발생시킨다. 본 연구에서는 이 문제를 개선하기 위하여 개발된 일반화 Poisson 확률분포 (GPD), 0-과잉 Poisson 확률분포 (ZIP), 0-과잉 일반화 Poisson 확률분포 (ZIGP), Bayesian 0-과잉 일반화 Poisson 확률분포 (Bayesian ZIGP)를 집중호우 자료에 적용하고, 5개 모형의 특성을 비교분석하였으며, Bayesian ZIGP 모형의 구축에 있어서는 정보적 사전분포를 사용함으로써 모형의 정확도를 개선하였다. 분석결과 분석하고자 하는 자료에 0이 과다하게 포함되어 있는 경우 POI 및 GPD 분포는 관측결과와는 다른 결과를 제시하여 적절한 모형으로 고려되지 못함을 알 수 있었다. 5가지 모형 중 정보적 사전분포를 탑재한 Bayesian ZIGP 모형이 가장 관측 자료와 유사한 결과를 도출하였으나 모형의 구축에 수반되는 실용적인 측면을 고려하면 ZIP 모형도 충분히 사용될 수 있는 모형으로 추천되었다.

• Journal of Applied Biological Chemistry
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• v.61 no.1
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• pp.83-91
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• 2018

Advances in bacterial and fungal genome mining uncover a plethora of cryptic secondary metabolite biosynthetic gene clusters. Guided by the genome information, targeted transcriptional derepression could be employed to determine the product of a cryptic gene cluster and to explore its biological role. Monascus spp. are food grade filamentous fungi popular in eastern Asia and several genome data belong to them are now available. We achieved transcription activation of a cryptic fungal polyketide synthase-nonribosomal peptide synthase gene Mpfus1 in Monascus purpureus ${\Delta}MpPKS5$ by inserting Aspergillus gpdA promoter at the upstream of Mpfus1 through double crossover gene replacement. The gene cluster with Mpfus1 show a high similarity to those for the biosynthesis of conjugated polyene derivatives with 2-pyrrolidone ring and the mycotoxin fusarin is the representative member of this group. The ${\Delta}MpPKS5$ is incapable of producing azaphilone pigment, providing an excellent background to identify chromogenic and UV-absorbing compounds. Activation of Mpfus1 resulted in a yellow hue on mycelia and its methanol extract exhibit a maximum absorption at 365 nm. HPLC analysis of the organic extracts indicated the presence of a variety of yellow compounds in the extract. This implies that the product of MpFus1 is metabolically or chemically unstable. LC-MS analysis guided us to predict the MpFus1 product and to propose that the Mpfus1-containing gene cluster encode the biosynthesis of a desmethyl analogue of fusarin. This study showcases the genome mining in Monascus and the possibility to unveil new biological activities embedded in it.

• The Korean Journal of Applied Statistics
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• v.24 no.5
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• pp.931-939
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• 2011

In practice, thresholds are determined by the two subjective assessment methods in a generalized pareto distribution of mean extreme function(MEF-graph) or Hill-graph. To remedy the problem of subjectiveness of these methods, we propose an alternative method to determine the threshold based on the robust statistics. We compared the MEF-graph, Hill-graph and our method through VaRs on the Korean stock market data from January 5, 1987 to August 3, 2009. As a result, the VaR based on the proposed method is not much different from the existing methods, and the standard deviation of VaR for our method was the smallest. The results show that our method can be a promising alternative to determine thresholds of the generalized pareto distributions.

• Mycobiology
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• v.31 no.1
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• pp.42-45
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• 2003

For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju $\beta$-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic $ura^-$ strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.

• The Korean Journal of Malacology
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• v.2 no.1
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• pp.30-34
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• 1986

Genetic variations of Semisulcospjra gottschei in Korea were investigated by means of starch gel-electrophoresis. The results are as follows; 1) Eight loci of ${\alpha}$-Gpd, Mdh-2, Pept-1, Pgm-2, Gp-1, Gp-2, Gp-3 and Gp-4 were monomorphic, and 8 loci of Mpi, Mdh-1, Pgi, Got, Pept-2, Pept-3, Pgm-1 and Sdh showed genetic variations. 2) Means of allele per locus ($\bar{A}$) and polymorphism (P) were revealed 0.64 and 0.44, respectively. The value of heterozygosity ($H_G$) showed a little more than heterozygosity $H_D$. ($H_D$=0.09, $H_G$=0.13) 3) Genetic variation appears to be due to loci of Got, Pept, Pgi, and Sdh, all of which of the $H_D$ values are high.

• Mycobiology
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• v.42 no.1
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• pp.12-16
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• 2014

We isolated and examined a new Alternaria sp., which causes leaf spots on Peucedanum japonicum in Korea, by using molecular and morphological methods. Phylogenetic analysis based on a combined internal transcribed spacer region analysis and two protein-coding genes (gpd and Alt a1) demonstrated that the causal fungus was most closely related to A. cinerariae and A. sonchi, and relevant to A. brassicae. However, conidial morphology indicated that it is a novel species within the genus Alternaria, and therefore we have assigned the fungus a new name in this study.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.447-450
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• 2005

Aspergillus niger inuE gene and Kluyveromyces marxianus INUI gene coding for exoinulinase were expressed in Saccharomyces cerevisiae under the control of K. marxianus INUI promoter. Recombinant S. cerevisiae expressing K. marxianus exoinulinase produced maximum 85 U/ml into culture medium, which was 9- to 14-fold higher than the activity produced by any other strain reported so far. In addition, K. marxianus INUI promoter produced 20- fold higher activity than S. cerevisiae glyceraldehydes phosphate dehydrogenase (GPD) promoter in S. cerevisiae.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.28 no.6
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• pp.375-382
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• 2016

In the present study, the wave hindcasting has been performed, and then the characteristics of storm waves at Gangnueng port was investigated, in which the high waves are observed. Comparing the numerical results with the wave measurements at Gangneung port, Niigata, and Hamada, there were good agreements between them. In particular, the Pearson correlation coefficients of significant wave heights and peak periods at Gangneung port were 0.92 and 0.72, respectively. Then the extreme wave analysis on the significant wave heights was carried out for the estimation of the frequency of storm waves. In this analysis, the storm waves over the threshold were fitted to GPD(Generalized Pareto Distribution). According to this analysis, the return period of the storm wave on February, 24, 2008, one of the large storm waves at Gangneung port, was 8.2 months. Among the computed significant wave heights larger than one-year wave, 58.3% of them were resulted from the storm, while the others were from the typhoon. Additionally, the regression analysis on the waves larger than one-month wave has been conducted, and then the relationship between the computed significant wave heights and the significant wave period, $T_{1/3}=7H_s^{0.25}$ was obtained.

• The Korean Journal of Malacology
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• v.12 no.1
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• pp.19-31
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• 1996

국내 11개 지역(경기도 옹진군 덕적도, 경기도 의정부시 소요산, 강원도 춘천시, 충청남도 태안군 안홍, 경상북도 울릉군 울릉도, 전라북도 부안군 변산, 전라남도 신안군 홍도, 흑산도 비금도, 진도, 제주도)에서 채집된 달팽이 (A. despecta sieboleiana)를 대상으로 외부형태 분석과 동위효소를 검출하여 각 지역 집단간의 형태적, 유전적 유연관계와 변이 정도를 연구한 결과는 다음과 같다. 외부형태에 의한 집단간 유연관계는 안홍과 덕적도집단(average taxonomic distance, D=0.358)이 형태적으로 가장 유사한 집단으로 나타났다. Polyacrylamide gel을 사용하여 11가지의 동위효소를 검출한 결과 AKP, ACP, AO, EST, GPD, HBDH, LDH, SDH, XDH의 동위효소에서 유전적 다형현상이 나타났다. 전 집단의 평균 다형형의 빈도는 59.19%이며 이형접합자 빈도는 0.263이고 각 집단의 유전적 차이치는 춘천과 울릉도 집단이 0.066(genetic distance)으로 가장 높은 유사성을 보였다. 지리적 분포에 따른 외부형태 변이와 유전적 변이와의 관계는 유의성이 없었다.

• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.