Mycobiology

The Korean Society of Mycology (한국균학회)
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Transformation of the Edible Basidiomycete, Pleurotus ostreatus to Phleomycin Resistance

  • Kim, Beom-Gi (Molecular Physiology Division, National Institute of Agricultural Biotechnology) ;
  • Joh, Jung-Ho (Department of Biochemistry, Kon-Kuk University) ;
  • Yoo, Young-Bok (Applied Microbiology Division, National Institute of Agricultural Science and Trechnology) ;
  • Magae, Yumi (Forestry and Forest Product Research Institute)
  • Published : 2003.03.31
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Abstract

For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju $\beta$-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic $ura^-$ strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.

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References

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