• Toxicological Research
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• v.17
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• pp.109-115
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• 2001

Abrus agglutinin was purified from the kernels of Abrus precatorius by Sepharose 4B affinity column chromatography followed by Sephadex G-100 gel filtration column chromatography. About 1.25 g of abrus agglutinin was obtained from 1 kg of the kernels. The LD$_{50}$ of abrus agglutinin is 5 mg/kg of body weight, which is less toxic than that of abrin, 20$\mu\textrm{g}$/kg body weight. The amino acid sequence of abrus agglutinin was determined by protein sequencing techniques and deduced from the nucleotide sequence of a cDNA clone encoding full length of abrus agglutinin. There are 258 residues, 2 residues and 267 residues in the A-chain, the linker peptide and the B-chain of abrus agglutinin, respectively. Abrus agglutinin had high homology to abrin-a (77.8%). The 13 amino acid residues involved in catalytic function, which are highly conserved among abrin and ricin, were also conserved within abrus agglutinin. The protein synthesis inhibitory activity of abrus agglutinin ($IC_{50}$/ = 3.5 nM) was weaker than that of abrin-a (0.05 nM). By molecular modeling followed by site-directed mutagenesis showed that Pro199 of abrus agglutinin A-chain located in amphipathic helix H and corresponding to Asn200 of abrin A-chain, can induce bending of helix H. This bending would presumably affect the binding of abrus agglutinin A-chain to its target sequence GpApGpAp, in the tetraloop structure of 285 r-RNA subunit and this could be one of major factors contributing to the relatively weak protein synthesis inhibitory activity and toxicity of abrus agglutinin.n.

• The Korean Journal of Physiology and Pharmacology
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• v.9 no.2
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• pp.109-115
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• 2005

Endothelial activation and subsequent recruitment of inflammatory cells are important steps in atherogenesis. The increased levels of cell adhesion molecules (CAM) have been identified in diabetic vasculatures, but the underlying mechanisms remain unclear. To determine the relationship among vascular production of superoxide, expression of CAM and diabetes, superoxide generation and expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E- and P-selectin in the aorta from control (C57BL/6J) and diabetic mice (ob/ob) were measured. In situ staining for superoxide using dihydroethidium showed an increased superoxide production in diabetic aorta, accompanied with an enhanced NAD(P)H oxidase activity. Immunohistochemical analysis revealed that the endothelial expression of ICAM-1 ($3.5{\pm}0.4$) and VCAM-1 ($3.8{\pm}0.3$) in diabetic aorta was significantly higher than those in control aorta ($0.9{\pm}0.5$ and $1.6{\pm}0.3$, respectively), accompanied with the enhanced expression of gp91phox, a membrane subunit of NAD(P)H oixdase. Furthermore, there was a strong positive correlation (r=0.89, P＜0.01 in ICAM-1 and r=0.88, P＜0.01 in VCAM-1) between ICAM-1/VCAM-1 expression and vascular production of superoxide. The present data indicate that the increased production of superoxide via NAD(P)H oxidase may explain the enhanced expression of CAM in diabetic vasculatures.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.5
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• pp.1284-1288
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• 2008

Recently, there is increasing the development requirement of middleware and interface for GPS(Global Positioning System), RFID(Radio Frequency IDentification), sensor networks. GPS supports a useful location based service. RFID supports products logistic and distribution services through the identification. A sensor network collects a context information, such as humidity, temperature and atmospheric pressure. This paper implements and verifies a integrated service middleware for supporting efficiently process of sensing data collected from RFID, GPS and sensor network. This middleware have the temporary store function, the redundancy exclusion function, certification function, the classification function and the database storage function. Additionary, this middleware connects with low-level adaptor using socket interface and supports the high-level application services using database connection. Therefore, user can develop easily various many ubiquitous application system using proposed middleware instead of each RFID middleware, GPS middleware and, middleware based on sensor network.

• Journal of Advanced Navigation Technology
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• v.16 no.3
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• pp.417-425
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• 2012

It is well known that GPS cannot provide positioning results if sufficient number of visible satellites are not available. To overcome this weak point, attentions have been recently moved to hybrid positioning methods that augments GPS with other sensors. As an extension of hybrid positiong methods, this paper proposes a new method that combines GPS and vision sensor to improve availability and accuracy of land vehicle positioning. The proposed method does not require any external map information and can provide position solutions if more than 2 navigation satellites are visible. To evaluate the performance of the proposed method, an experiment result with real measurements is provided and a result shows that accumulated error of n-axis is almost 2.5meters and that of e-axis is almost 3meters in test section.

• Journal of the Korean Association of Geographic Information Studies
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• v.8 no.1
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• pp.88-95
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• 2005

Direct Georeferencing by combined multi-sensor based on the direct measurement of the projection centers and rotation angle of sensor through loading the GPS and INS in aircraft. The method of combined multi-sensor can offer us to acquire the exterior orientation parameters with only minimum GCPs, even the ground control process could be completely skipped. Consequently, It is possible extreme to reduce the time and expense for the mapping process. In this study, a CCD camera is simultaneously used in combined multi-sensor surveying, and acquired CCD image through Direct Georeferencing produce digital orthoimage. In this process, methods of combining sensor and digital orthoimage are examined and estimated. For the comparison of the positioning accuracy digital orthoimage through Direct Georeferencing, GCPs determined by GPS surveying are used. Two digital orthoimage are produced; one with a few GCP and the other without them. The accuracy of orthoimages produced through combined multi-sensor with GCPs meets that of 1:1,000 maps. Without GCPs, it meets that of 1:5,000 maps.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.16 no.5
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• pp.109-120
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• 2017

It is important to estimate objectively in the driving test. Especially, the driving test is examined by totally driving ability, rule observation and situational judgement. For this, a grading automation system for driving test was presented by using GPS, sensor data and equipment operation informations. This system is composed of vehicle mounted module, automatic grading terminal, data controller, data storage and processing server. The vehicle mounted module gathters sensor data in the car. The terminal performs automatic grading using the received sensor data according the driving test criterion. To overcome the misposition of vehicle in the map due to GPS error, we proposed the automatic grading system by map matching method, path deviation and return algorithm. In the experimental results, it was possible to grade automatically, display the right position of the car, and return to the right path under 10 seconds when the vehicle was out of the shadow region of the GPS. This system can be also applied to the driving education.

• Journal of Korean Society for Geospatial Information Science
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• v.13 no.1 s.31
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• pp.11-17
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• 2005

A lot of small-scale reservoirs that is extravagant in whole country are controlled state or real condition that correct data about the practice contents is not equipped all up to now. In this study, YungNam districts that region Gyeong-Ju city reservoir is distributed much of when flow speedily choose 23 peace among 73 small scale reservoir and experimented. Acquired data every 5 seconds, and user E/S and user value TS for orthocenter to 1 second interval and executes present condition measurement using RTK-GPS. Could was wave and state that reservoir of 15 places (about 65%) gives protective care that do 3D modelling with acquired data, and now correct contents about reservoir state past through comparative analysis with data. Also, area of full water and wave that analyze effective storage and area of full water of 23 places reservoir could know that 20.63%, effective storage decreased as 3.34%. I wish to contribute about analysis about present reservoir state through this and method the adminstration direction.

• Journal of Pharmaceutical Investigation
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• v.37 no.5
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• pp.297-303
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• 2007

The purposes of this study were to clarify the involvement of P-glycoprotein (P-gp) in the efflux of levosulpiride in knockout mice that lack the mdr1a1b gene and to evaluate the relationship between the genetic polymorphisms in MDR1 gene (exon 21) and levosulpiride disposition in healthy Korean subjects. After oral administration ($10\;{\mu}g/g$) of levosulpiride to mdr1a/1b(-/-) and wild-type mice, plasma and brain samples were obtained at 45 min. We also investigated the genotype for MDR1 (exon 21) gene in humans using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. A single oral dose of 25 mg levosulpiride was administered to 58 healthy subjects, who were based on the MDR1 genotype for the G2677T SNP. Blood samples were taken up to 36 hr after dosing. The concentrations of levosulpiride in mouse plasma and brain were statistically significant difference between the two animal groups (P<0.05). In addition, the average brain-to-plasma concentration ratio (Kp) of levosulpiride was 3.4-fold (P<0.01) higher in the mdr1a/1b(-/-) mice compared with the wild-type mice. We also found that the values of $AUC_{0-{\infty}$, partial AUC ($AUC_{0-4h}$) and $C_{max}$ were significantly different between homozygous 2677TT subjects and the subjects with at least one wild-type allele (GG and GT subjects, P=0.012 for $AUC_{0-{\infty}$; P=0.008 for $AUC_{0-4h}$; P=0.038 for $C_{max}$). The results confirm that levosulpiride is a P-gp substrate in vivo, and clearly demonstrate the effect of SNP 2677G>T in exon 21 of the MDR1 gene on levosulpiride disposition.

• Journal of the Korean earth science society
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• v.21 no.4
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• pp.380-388
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• 2000

The estimation of tropospheric zenith delay over the Seoul-Jecheon area using GPS is presented. Over the past ten years, the world-wide industrial nations have been intensively concerned over increasing GPS surveyings in the various fields of earth science. To preserve precise positioning under various weather conditions, relationships between tropospheric zenith delay and GPS accuracy are analyzed. GPS accuracies are compared with tropospheric zenith delay produced by Bernese 4.0 software. Errors of tropospheric delay are 20cm in mean and reduced up to 5cm when tropospheric correction models are used. Correlation between error of GPS and tropospheric zenith delay plays a positive role to monitor the migration of weather front in the established Korean GPS network.

• KIPS Transactions on Software and Data Engineering
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• v.2 no.5
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• pp.347-352
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• 2013

In impulse noise filtering techniques window size play an important role. Usually, an appropriate window is determined according to the noise density. A small window may not be able to suppress noise properly whereas a large window may remove edges and fine image details. Moreover, the value of the central pixel is estimated by considering all pixels within the window. In this work, contrary to the previous approaches, we propose an iterative impulse noise removal scheme that emphasizes on noise-free pixels within a small neighborhood. The iterative process continues until all noisy pixels are replaced with the estimated pixels. In order to estimate the optimal value for a noisy pixel, a genetic programming (GP) based estimator is evolved that takes few noise-free pixels as input. The estimator is constituent of noise-free pixels, arithmetic operators and random constants. Experimental results show that theproposed scheme is capable of removing impulse noise effectively while preserving the fine image details. Especially, our approach has shown effectiveness against high impulse noise density.