• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.51-55
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• 2016

Phytochemical investigation of Pistacia integerrima has highlighted isolation of two known compounds naringenin (1) and dihydrokaempferol (2). A crude extract and these isolated compounds were here evaluated for their effects on reversion of multidrug resistance (MDR) mediated by P-glycoprotein (P-gp). The multidrug resistance P-glycoprotein is a target for chemotherapeutic drugs from cancer cells. In the present study rhodamine-123 exclusion screening test on human mdr1 gene transfected mouse gene transfected L5178 and L5178Y mouse T-cell lymphoma cells showed excellent MDR reversing effects in a dose dependent manner. In-silico molecular docking investigations demonstrated a common binding site for Rhodamine123, and compounds naringenin and dihydrokaempferol. Our results showed that the relative docking energies estimated by docking softwares were in satisfactory correlation with the experimental activities. Preliminary interaction profile of P-gp docked complexes were also analysed in order to understand the nature of binding modes of these compounds. Our computational investigation suggested that the compounds interactions with the hydrophobic pocket of P-gp are mainly related to the inhibitory activity. Moreover this study s a platform for the discovery of novel natural compounds from herbal origin, as inhibitor molecules against the P-glycoprotein for the treatment of cancer.

• Animal Bioscience
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• 제35권9호
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• pp.1379-1389
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• 2022

Objective: This study identified the major lactic acid bacteria (LAB) strains from different fermented total mixed rations (FTMRs) via metataxonomic analysis and evaluated the ability of their standard strain as ensiling inoculants for corn stover silage. Methods: The bacterial composition of eight FTMRs were analyzed by 16S rDNA sequencing. Corn stover was ensiled without LAB inoculation (control) or with 1×10⁶ cfu/g LAB standard strain (Lactobacillus vaginalis, Lactobacillus reuteri, Lactobacillus helveticus, or Lactobacillus paralimentarius) selected from the FTMRs or 10 g/t commercial silage inoculant (CSI) around 25℃ for 56 days. For each inoculation, a portion of the silage was sampled to analyze ensiling characteristics at time intervals of 0, 1, 3, 7, 14, 28, and 56 days, gas production (GP), microbial crude protein and volatile fatty acids as the measurements of rumen fermentation characteristics were evaluated in vitro with the silages of 56 days after 72 h incubation. Results: Lactobacillus covered >85% relative abundance of all FTMRs, in which L. pontis, L. vaginalis, L. reuteri, L. helveticus, and L. paralimentarius showed >4% in specific FTMRs. CSI, L. helveticus, and L. paralimentarius accelerated the decline of silage pH. Silage inoculated with L. paralimentarius and CSI produced more lactic acid the early 14 days. Silage inoculated with L. paralimentarius produced less acetic acid and butyric acid. For the in vitro rumen fermentation, silage inoculated with CSI produced more potential GP, isobutyric acid, and isovaleric acid; silage inoculated with L. helveticus produced more potential GP and isovaleric acid, silage inoculated with L. paralimentarius or L. reuteri produced more potential GP only. Conclusion: The standard strain L. paralimentarius (DSM 13238) is a promising ensiling inoculant for corn stover silage. The findings provide clues on strategies to select LAB to improve the quality of silage.

• Korean Journal of Radiology
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• 제23권1호
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• pp.60-67
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• 2022

Objective: To categorize multiparametric MRI features of Bacillus Calmette-Guérin (BCG)-related granulomatous prostatitis (GP) and discover potential manifestations for its differential diagnosis from prostate cancer. Materials and Methods: The cases of BCG-related GP in 24 male (mean age ± standard deviation, 66.0 ± 9.4 years; range, 50-88 years) pathologically confirmed between January 2011 and April 2019 were retrospectively reviewed. All patients underwent intravesical BCG therapy followed by a MRI scan. Additional follow-up MRI scans, including diffusion-weighted imaging (DWI), were performed in 19 patients. The BCG-related GP cases were categorized into three: A, B, or C. The lesions with diffusion restriction and homogeneous enhancement were classified as type A. The lesions with diffusion restriction and a poorly enhancing component were classified as type B. A low signal intensity on high b-value DWI (b = 1000 s/mm²) was considered characteristic of type C. Two radiologists independently interpreted the MRI scans before making a consensus about the types. Results: The median lesion size was 22 mm with the interquartile range (IQR) of 18-26 mm as measured using the initial MRI scans. The lesion types were A, B, and C in 7, 15, and 2 patients, respectively. Cohen's kappa value for the inter-reader agreement for the interpretation of the lesion types was 0.837. On the last follow-up MRI scans of 19 patients, the size decreased (median, 5.8 mm; IQR, 3.4-8.5 mm), and the type changed from A or B to C in 11 patients. The lesions resolved in four patients. In five patients who underwent prostatectomy, caseous necrosis on histopathology matched with the non-enhancing components of type B lesions and the entire type C lesions. Conclusion: BCG-related GP demonstrated three imaging patterns on multiparametric MRI. Contrast-enhanced T1-weighted imaging and DWI may play a role in its differential diagnosis from prostate cancer.

• Bulletin of the Korean Chemical Society
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• 제33권11호
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• pp.3857-3860
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• 2012

• 대한본초학회지
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• 제28권4호
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• pp.17-23
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• 2013

Objectives : The objective of the paper was to study cutting method of Gardeniae frutus, that tends to show symptom of separations in a packing, in accordance with the comparison experiment of quantification and antioxidant effect proceed with the intact Gardeniae fructus(Gf), seed of Gardeniae fructus (Gs) and the pericarp of Gardeniae fructus (Gp) separately. Methods : The Gf, Gs, and Gp were extracted using 80% MeOH, followed by quantizing geniposide contained in each group. A MTT assay was conducted and ROS generation and NO production were measured for comparing its antioxidant effect. Results : As a result of quantizing geniposide contained in the Gf, Gp, and Gs, respectively, the geniposide content was shown to be the highest in the Gs. MTT assay showed that no cytotoxicity was observed in the groups treated with Gf, Gp, and Gs, respectively, at a dose of 500 ${\mu}g/ml$. The ROS generation was shown to have more significantly decreased in the group pretreated with Gf 500 ${\mu}g/ml$ than in the group treated with LPS. The NO level was shown to have more significantly decreased in the group pretreated with Gp 500 ${\mu}g/ml$ than in the group treated with LPS. Conclusion : As the geniposide content and antioxidant effect of Gf varies according to its each part, it is recommended that Gf should be distributed as an intact form other than segregation in packing.

• 한국위성정보통신학회논문지
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• 제2권1호
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• pp.48-55
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• 2007

본 논문은 위성항법지상국시스템 개발을 위하여 시스템 요구사항 정의, 시스템 구성, 시스템에 대한 주요 파라미터별 요구사항 도출 및 분석 결과를 기술한 논문이다. 위성항법 지상국 시스템은 항법 위성인 GPS와 Galileo 위성으로부터 항법 신호를 감시하는 신호감시국과 처리된 위성항법 데이터를 갈릴레오 위성으로 송신하는 상향링크국, 신호감시국 및 상향링크국을 구성하는 장비들에 대한 감시 및 제어기능을 수행하는 감시 및 제어시스템으로 이루어진다. 신호감시국은 항법 수신기와 원자시계, 기상정보처리 및 항법데이타 처리를 담당하는 서브시스템으로 구성되며 항법 수신기는 GPS와 Galileo 위성으로부터 항법 신호를 수신할 수 있는 복합 수신기 형태가 된다. 신호감시국은 항법 정보에 대한 보정정보처리을 위해 위성항법 제어센터인 GCC(GNSS Control Center)와 인터페이스 될 수 있어야 한다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.27.2-27.2
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• 2008

• Structural Engineering and Mechanics
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• 제71권4호
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• pp.329-339
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• 2019

Assessment of failure probability, especially for a complex structure, requires a considerable number of calls to the numerical model. Reliability methods have been developed to decrease the computational time. In this approach, the original numerical model is replaced by a surrogate model which is usually explicit and much faster to evaluate. The current paper proposed an efficient reliability method based on Monte Carlo simulation (MCS) and multi-gene genetic programming (MGGP) as a robust variant of genetic programming (GP). GP has been applied in different fields; however, its application to structural reliability has not been tested. The current study investigated the performance of MGGP as a surrogate model in structural reliability problems and compares it with other surrogate models. An adaptive Metropolis algorithm is utilized to obtain the training data with which to build the MGGP model. The failure probability is estimated by combining MCS and MGGP. The efficiency and accuracy of the proposed method were investigated with the help of five numerical examples.

• Steel and Composite Structures
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• 제31권5호
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• pp.427-435
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• 2019

The estimation of moment and rotation in steel rack connections could be significantly helpful parameters for designers and constructors in the initial designing and construction phases. Accordingly, Extreme Learning Machine (ELM) has been optimized to estimate the moment and rotation in steel rack connection based on variable input characteristics as beam depth, column thickness, connector depth, moment and loading. The prediction and estimating of ELM has been juxtaposed with genetic programming (GP) and artificial neural networks (ANNs) methods. Test outcomes have indicated a surpass in accuracy predicting and the capability of generalization in ELM approach than GP or ANN. Therefore, the application of ELM has been basically promised as an alternative way to estimate the moment and rotation of steel rack connection. Further particulars are presented in details in results and discussion.

• 대한임상약리학회:학술대회논문집
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• 대한임상약리학회 2007년도 추계학술대회
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• pp.88-88
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• 2007

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