• Journal of Life Science
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• v.26 no.3
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• pp.296-301
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• 2016

An antibacterial peptide from skin extract of the catfish Silurus asotus was purified and characterized. The acidified skin extract was put through a Sep-Pak C18 solid phase extraction cartridge using a stepwise gradient and divided into flow-through (F.T.), 10% methanol-elute (RM10), 60% methanolelute (RM60), and 100% methanol-elute (RM100) fractions. RM10, RM60, and RM 100 showed antimicrobial activity against Escherichia coli D31. On the other hand, the F.T. fraction did not show antimicrobial activity. Among the various fractions, RM 60 had the highest activity. RM 60 was partially purified on a cation exchange column (CM52) by a stepwise gradient. The ammonium acetate (pH 5.15) 0.02 M – 0.8 M fraction showed antimicrobial activity. Then an antimicrobial peptide was purified using a 0.6M fraction with strong antibacterial activity through a series of five C18 reversed-phase HPLC columns. For the characterization of the purified peptide, the molecular weight and amino acid sequence were analyzed by MALDI-TOF MS and Edman degradation. The molecular weight of this peptide was about 4182.1 [M+H]⁺. The amino acid sequence of this peptide was partially determined as follows: PALXXKARREAKVKF. These findings suggest that this peptide plays a significant role in the innate defense system of catfish skin.

• Journal of Life Science
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• v.27 no.1
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• pp.50-56
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• 2017

An antibacterial peptide was purified from an acidified gill extract of the pufferfish Takifugu pardalis. The acidified gill extract was put through a Sep-Pak C18 solid phase extraction cartridge using a stepwise gradient and divided into a flow-through (F.T.) and 60% methanol fraction (RM 60). Among the eluents, RM 60 had potent antibacterial activity against Bacillus subtilis KCTC 1021. RM 60 was partially purified on a cationic-exchange column (SP-5PW) by a linear gradient, and the antibacterial peptide was then further purified, using a series of cationic-exchange and $C_{18}$ reversed-phase HPLC columns. For characterization of the purified peptide, its molecular weight and amino acid sequence were analyzed by MALDI-TOF MS and Edman degradation. The molecular weight of the peptide was about 1171.6 Da. The amino acid sequence of the peptide was partially determined as: STKEKAPRKQ. A comparison of the N-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high homology with the N-terminus of the histone H3 protein, which belongs to the histone H3 family. Thus, this peptide was designated as a puffer fish gill (PFG)-related antimicrobial peptide. This is the report to describe an antimicrobial function for the N-terminus of histone H3 of an animal species. The findings suggest that this peptide plays a significant role in the innate defense system of the pufferfish.

• Journal of Korean Society of Transportation
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• v.25 no.6
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• pp.185-196
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• 2007

The aim of this research is to develop a signal control algorithm using internal metering to minimize total delay that vehicles go through, in case a network is oversaturated. To calculate total delay on the network, the authors first detect vehicles' arrivals and departures in the network through the detecting system, and chase the vehicles' flow in the links with a platoon dispersion model. Following these, the authors calculate the queue length in all the inks of the network through the chase of vehicles, deduce the stopped time delay, and finally convert the stopped time delay to the approach delay with a time-space diagram. Based on this calculated delay, an algorithm that calculates the level of the internal metering necessary to minimize the deduced approach delay is suggested. To verify effectiveness of this suggested algorithm, the authors also conduct simulation with the micro-simulator VISSIM. The result of the simulation shows that the average delay per vehicle is 82.3 sec/veh and this delay is lower than COSMOS (89.9sec/veh) and TOD (99.1sec/veh). It is concluded that this new signal control algorithm suggested in this paper is more effective in controlling an oversaturated network.

• Journal of Korean Society of Water and Wastewater
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• v.29 no.4
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• pp.447-457
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• 2015

Water cycle within the human civilization has become important with urbanization. To date, water cycle in the eco-system has been the focus in identifying the degree of water cycle in cities, but in practicality, water cycle within the human civilization system is taking on an increasing importance. While in recent years plans to reuse water have been implemented to restore water cycle in cities, the effect that such reuse has on the entire water cycle system has not been analyzed. The analysis on the effect that water reuse has on urban areas needs to be go beyond measuring the cost-savings and look at the changes brought about in the entire city's water cycle system. This study uses a SWAT model and water balance analysis to review the effects that water reuse has on changes occurring in the urban water cycle system by linking the water cycle within the eco-system with that within the human civilization system. The SWAT model to calculate the components of water cycle in the human civilization system showed that similar to measured data, the daily changes and accumulative data can be simulated. When the amount of water reuse increases in urban areas, the surface outflow, amount of sewer discharge and the discharged amount from sewage treatment plants decrease, leading to a change in water cycle within our human civilization system. The determinant coefficients for reduced surface outflow amount and reduced sewer discharge were 0.9164 and 0.9892, respectively, while the determinant coefficient for reduced discharge of sewage treatment plants was 0.9988. This indicates that with an increase in water reuse, surface flow, sewage and discharge from sewage treatment plants all saw a linear reduction.

• The Journal of Internal Korean Medicine
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• v.39 no.3
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• pp.372-388
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• 2018

Objective: This study evaluates the effectiveness and safety of moxibustion for benign prostatic hyperplasia (BPH). Methods: Using the keywords "benign prostatic hyperplasia", "benign prostatic hypertrophy", "benign prostatic enlargement", "prostatic hyperplasia", and "moxibustion", we searched papers in numerous databases, including National Discovery for Science Leaders (NDSL), Korean Traditional Knowledge Portal (KTKP), Oriental Medicine Advanced Searching Integrated System (OASIS), Research Information Sharing Service (RISS), PubMed, Embase, and CENTRAL. The search range included randomized controlled trials (RCTs). Papers not matched with inclusion criteria were excluded. The methodological quality of each RCT was assessed using the Cochrane risk-of-bias tool. Where appropriate, meta-analyses were performed. Results: Initially, 77 studies were found. Of these, 11 duplicate studies were removed and 27 were excluded following title and abstract screening. After the remaining 39 papers were scanned, 13 RCTs were selected and analyzed. Among these 13 RCTs, five compared moxibustion therapy and oral medication, seven compared moxibustion plus acupuncture therapy and oral medication, and one compared moxibustion plus acupuncture therapy and sham-moxibustion. The meta-analysis showed positive results for the use of moxibustion therapy in terms of International Prostate Symptom Score (IPSS), Quality Of Life (QOL), Maximum Flow Rate (Qmax), Prostate Volume (PV), and the efficacy rate. The meta-analysis showed positive results for the use of moxibustion plus acupuncture therapy in terms of IPSS, QOL, and the efficacy rate. Conclusions: This meta-analysis of clinical trials suggests that moxibustion is effective intreating BPH patients. The results of this study could be applied to clinical treatment of BPH. However, additional large-scale clinical researches should be conducted.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.10
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• pp.3004-3010
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• 2009

The Korean pop music people enjoy today is mostly based on the pop music from foreign countries. Therefore, not only is the history very short, but also not much research is done in the Korean pop music field. Especially in terms related with the singing method, in other words the breathing and vocalization of Korean singers, it is difficult to define where it is actually situated. To add a little, it seems as if it is swarming somewhere in the middle of Korean traditional music singing methods and classical music vocalization. As pop music made its way into Korea, Korean singers had to go through many trial and errors to sing the songs of singers that had different physical conditions. Singers getting vocal nodules because of excessive vocalization, was not a surprise at that time. Yongphil Jo, a Korean singer, wanted to overcome his limits and maximize his vocal abilities. He found the answers in Korean traditional music. One of his representative songs, "The lady out the window", is one of Jo's title songs and it is the song that made a turning point in the Korean pop music vocalization. The purpose of this research is to understand the circumstances of Korean pop music through the periodical and musical background of the past and find out why the song (The lady out the window) was such an epochal vocal method at that time. Moreover, this paper covers how the breathing and vocalization is expressed by analyzing the flow, the emotional expressions, and the techniques of "The lady out the window."

• Resources Recycling
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• v.22 no.2
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• pp.22-28
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• 2013

In the present study, the possibility of recovering and recycling the silicon carbide(SiC) from a silicon sludge by removing Fe and Si impurities was investigated. Si and SiC were separated from the silicon sludge using centrifugation. The separated SiC concentrate consisted of Fe, Si and SiC, in which Fe and Si were removed to recover the pure SiC. Leaching with acid/alkali solution was compared with the vapor-phase chlorination. The Fe concentration removed in the SiC was 49 ppm, and it was separated by leaching with 1 M HCl solution at $80^{\circ}C$ for 1 h. The Si concentration removed in the SiC was 860 ppm, and it was separated by leaching with 1M NaOH solution at $50^{\circ}C$ for 1 h. The SiC concentrate was chlorinated in a tubular reactor, 2.4 cm in diameter and 32 cm in length. The boat filled with SiC concentrate was located at the midpoint of the alumina tube, then, the chlorine and nitrogen gas mixture was introduced. The Fe and Si concentration removed in the SiC were 48 ppm and 405 ppm, respectively, at $500^{\circ}C$ reactor temperature, 4 h reaction time, 300 cc/min gas flow rate, and 10% $Cl_2$ gas mole fraction.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8533-8539
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• 2016

Background: B-cell chronic lymphocytic leukemia B (B-CLL), the most common type of leukemia, may be caused by apoptosis deficiency in the body. Adipose tissue-derived mesenchymal stem cells (AD-MSCs) as providers of pro-apoptotic molecules such as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), can be considered as an effective anti-cancer therapy candidate. Therefore, in this study we assessed the role of tumor necrosis factor-producing mesenchymal stem cells oin apoptosis of B-CLL cells resistant to fludarabine-based chemotherapy. Materials and Methods: In this study, after isolation and culture of AD-MSCs, a lentiviral LeGO-iG2-TRAIL-GFP vector containing a gene producing the ligand pro-apoptotic with plasmid PsPAX2 and PMDG2 virus were transfected into cell-lines to generate T293HEK. Then, T293HEK cell supernatant containing the virus produced after 48 and 72 hours was collected, and these viruses were transduced to reprogram AD-MSCs. Apoptosis rates were separately studied in four groups: group 1, AD-MSCs-TRAIL; group 2, AD-MSCs-GFP; group 3, AD-MSCs; and group 4, CLL. Results: Observed apoptosis rates were: group 1, $42{\pm}1.04%$; group 2, $21{\pm}0.57%$; group 3, $19{\pm}2.6%$; and group 4, % $0.01{\pm}0.01$. The highest rate of apoptosis thus occurred ingroup 1 (transduced TRAIL encoding vector). In this group, the average medium-soluble TRAIL was 72.7pg/m and flow cytometry analysis showed a pro-apoptosis rate of $63{\pm}1.6%$, which was again higher than in other groups. Conclusions: In this study we have shown that tumor necrosis factor (TNF) secreted by AD-MSCs may play an effective role in inducing B-CLL cell apoptosis.

• The Research Journal of the Costume Culture
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• v.6 no.1
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• pp.1-13
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• 1998

As for the taboo of dress and its ornaments which was applied to only populace one-sidedly mainly under the power structure of feudalistic centralization of government like Chosun dynasty, the aspect of taboo was diversified all the more by having relation with various phenomenon of social structure characteristics, and the taboo items to be given in accordance with the flow of time shows the diversity. Especially, the structure of dress and its ornaments in Chosun period showed the side of social position relation and luxury control, and the taboo of dress and its ornaments which was derived from the current thought about China was given frequently. To respect the frugal virtue and moderate the life has relation with the stream of the times in individuals or nations. And, that it is externalized is dress and its ornaments. As the luxury about dress and its ornaments was serious in accordance with times, it appeared by having relation with whole economic question of nations. Then, this researcher thought this by relating this to the culture of customs. This researcher thought that the luxury about clothing gets to go to extremes and the law and order collapses, according as public morals get to be very lax. And, it can be said that the way to be able to control this is that the customs should be thoroughly obeyed and that the system to be able to play the role of braking is taboo. So, this researcher thinks that it is significant to examine the achievement which has contributed toward the life of dress and its ornaments by successive kinds on the basis of a true record of the Vidynasty and other all sorts of literatures about the real situation of prohibition order which has been from the first king to Kojong at the last period of nation. The criticism and taboo about luxury which have been discussed at Chosun period may be subdivided into some kinds in accordance with the subjects. But, in this tests, as it sis the real situation that the case to be due to the lavish necessary articles for marriage which is coming to fore as our social problem nowadays is increasing remarkably, this researcher tries to examine the taboo about the marriage luxury at Chosun period in the order of successive generations. Thus, before examining the taboo of marriage luxury, this researcher tries to examine about the taboo of textile luxury and study the taboo of marriage luxury, as the marriage luxury governs almost everything of textile luxury and it is regarded as the origin of marriage luxury, especially. And, this researcher tries to study as to how was the penal regulations based on it at the same time.

• Journal of Navigation and Port Research
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• v.38 no.2
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• pp.155-162
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• 2014

In container terminals, stacking yard is the place where import and export containers are temporarily stored before being loaded onto or after being discharged from a ship. Since all the containers go through the stacking yard in their logistic flow, the productivity of the terminal critically depends on efficient operation of stacking yard, which again depends on how well the stacking locations of the incoming containers are determined. However, a good location for stacking an incoming container later can turn out to be a bad one when that container is to be fetched out of the stacking yard, especially if some rehandling is required. This means that good locations for the containers are changing over time. Therefore, in most container terminals, the so-called remarshaling is done to move the containers from bad location to good locations. Although there are many previous works on remarshaling, they all assume that the remarshaling can be done separately from the main jobs when the cranes are idle for rather a long period of time. However, in reality, cranes are hardly available for a period long enough for remarshaling. This paper proposes a crane dispatching strategy that allows remarshaling jobs to be mixed together with the main jobs whenever an opportunity is detected. Experimental results by simulation reveals that the proposed method effectively contributes to the improvement of terminal productivity.