• Journal of Magnetics
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• v.15 no.4
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• pp.194-198
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• 2010

In our present work, we developed a GMI (giant magnetoimpedance) sensor system to detect magnetic fields in the milli gauss range based on the asymmetric magnetoimpedance (AGMI) effect in Co-based amorphous ribbon with self bias field produced by field-annealing in open air. The system comprises magnetoimpedance sensor probe, signal conditioning circuits, A/D converter, USB controller, notebook computer, and program for measurement and display. Sensor probe was constructed by wire-bonding the cobalt based amorphous ribbon with dimensions $10\;mm\;{\times}\;1\;mm\;{\times}\;20\;{\mu}m$ on a printed circuit board. Negative feedback was used to remove the hysteresis and temperature dependence and to increase the linearity of the system. Sensitivity of the milli gauss meter was 0.3 V/Oe and the magnetic field resolution and environmental noise level were less than 0.01 Oe and 2 mOe, respectively, in an unshielded room.

• Proceedings of the Korea Information Processing Society Conference
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• 2024.05a
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• pp.69-72
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• 2024

모델 역추론 공격은 공격 대상 네트워크를 훈련하기 위해 사용되는 훈련 데이터셋 중 개인 데이터셋을 공개 데이터셋을 사용하여 개인 훈련 데이터셋을 복원하는 것이다. 모델 역추론 방법 중 적대적 생성 신경망을 사용하여 모델 역추론 공격을 하는 과거의 논문들은 딥러닝 모델 전체의 역추론에만 초점을 맞추기 때문에, 이를 통해 얻은 원본 이미지의 개인 데이터 정보는 제한적이다. 따라서, 본 연구는 대상 모델의 중간 출력을 사용하여 개인 데이터에 대한 더 품질 높은 정보를 얻는데 초점을 맞춘다. 본 논문에서는 적대적 생성 신경망 모델이 원본 이미지를 생성하기 위해 사용되는 계층별 역추론 공격 방법을 소개한다. MNIST 데이터셋으로 훈련된 적대적 생성 신경망 모델을 사용하여, 원본 이미지가 대상 모델의 계층을 통과하면서 얻은 중간 계층의 출력 데이터를 기반으로 원본 이미지를 재구성하고자 한다. GMI 의 공격 방식을 참고하여 공격 모델의 손실 함수를 구성한다. 손실 함수는 사전 손실 및 정체성 손실항을 포함하며, 역전파를 통해서 원본 이미지와 가장 유사하게 복원할 수 있는 표현 벡터 Z 를 찾는다. 원본 이미지와 공격 이미지 사이의 유사성을 분류 라벨의 정확도, SSIM, PSNR 값이라는 세 가지 지표를 사용하여 평가한다. 공격이 이루어지는 계층에서 복원한 이미지와 원본 이미지를 세 가지 지표를 가지고 평가한다. 실험 결과, 공격 이미지가 원본 이미지의 대상 분류 라벨을 정확하게 가지며 원본 이미지의 필체를 유사하게 복원하였음을 보여준다. 평가 지표 또한 원본 이미지와 유사하다는 것을 나타낸다.

• Economic and Environmental Geology
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• v.50 no.4
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• pp.287-302
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• 2017

The carbonate rocks of the Daegi Formation are composed of the limestone at the upper and lower zones, and the dolomite at the middle zone, in which the upper zone has higher CaO content than others. The colors of carbonate rock in the Daegi Formation can be divided into five types; white, light brown, light gray, gray, and dark gray. The white to light gray colored rocks correspond to the high purity limestone with 53.15 ~ 55.64 wt. % CaO, and the light brown colored rocks contain 20.71 ~ 21.67 wt. % MgO. The bleaching of carbonate rocks are not related to CaO composition of the rocks, as light gray rocks tend to be higher in CaO content than those of the white rocks at the lower zone. The pelitic components are also occasionally increased in white limestone than light grey one. $Al_2O_3$ is one of the most difficult content to remove during hydrothermal processes, so the interpretation that the limestone is purified together with hydrothemral bleaching, has little merit. The wide range (over 16 ‰) of ${\delta}^{18}O_{SMOW}$, smaller variation (within 2 ‰) of ${\delta}^{13}C_{PDB}$ are apparent in both the upper and lower zones, which indicate the Daegi Formation had been affected overall by hydrothermal fluids. The K-Ar isotopic age of hydrothermal alteration in the GMI limestone mine is $85.1{\pm}1.7Ma$. Gradual change from grey through light grey to white limestone is accompaned by lower oxygen stable isotope values, which is major evidence that the hydrothermal effect is the main process of the bleaching. Although the Daegi Formation has suffered from hydrothermal activity and increase in whiteness, there is no clear evidence demonstrating the relationship between bleaching and high purity of limestone. The purification of limestone has nothing to do with the hydrothermal activity in this area. Instead, it should be considered that the change of sedimentary environment related to see-level fluctuation which can prevent deposition of pelitic components especially $Al_2O_3$ contrbuted to the formation of the high purity limestone in the upper zone of the Daegi Formation. Considering the evidences such as increase in CaO content of limestone by depth, gradual change from calcite to dolomite at the lower zones, and occurring the high purity limestone at the upper zone, the interpretation of sequence stratigraphic aspect to the formation of the high purity Daegi limestone appears to be more suitable than that of hydrothermal alteration origin.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.2
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• pp.171-175
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• 2006

The aim of this in vitro study was to test the effect of microinjection (Mi) of foreign gene into the rabbit egg pronucleus and epidermal growth factor (EGF) addition on the blastocyst rate, the cell number and the diameter of embryos, and to determine possible relationships between embryo cell number and embryo diameter. Blastocyst rate was significantly decreased in gene- Mi (G-Mi/E0) group (63.1%) comparing to intact ones (83.5%, $p_1$<0.05). The addition of EGF at 20ng/ml (G-Mi/E20) or 200 ng/ml (GMi/ E200) to gene-Mi embryos did not affect blastocyst rate (65.6 and 55.2% resp.). As a control for Mi, the eggs were microinjected with the same volume of phosphate-buffered solution (PBS-Mi) instead of the gene construct solution. Cell numbers and embryo diameters were measured from embryo images obtained on confocal laser scanning microscope. Bonferroni-modified LSD test showed that the embryo cell number in PBS-Mi group was significantly lower ($p_1$<0.05) and in gene-Mi group was tended to decrease compared with intact embryos. Embryo diameter was not different among experimental groups. No effect of EGF given at any doses both on the cell number and embryo diameter was found. A positive correlation between cell number and embryo diameter was observed in all groups of embryos. Since embryo diameter was not changed under the influence of Mi or EGF addition in this study, this seems to be more conservative characteristics of the embryo morphology. These results suggest that the pronuclear microinjection compromises developmental potential of embryos, decreasing blastocyst rate and embryo cell number, whilst embryo diameter is not affected. No effects of EGF on studied parameters were confirmed. Declined quality of Mi-derived embryos is caused by the microinjection procedure itself, rather than by the gene construct used.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.550-557
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• 2010

Escherichia coli (E. coli) heat-labile enterotoxin B subunit (LTB) was regarded as one of the most powerful mucosal immunoadjuvants eliciting strong immunoresponse to coadministered antigens. In the research, the high-level secretory expression of functional LTB was achieved in P. pastoris through high-density fermentation in a 5-1 fermentor. Meanwhile, the protein was expressed in E. coli by the way of inclusion body, although the gene was cloned from E. coli. Some positive yeast and E. coli transformants were obtained respectively by a series of screenings and identifications. Fusion proteins LTB-6$\times$His could be secreted into the supernatant of the medium after the recombinant P. pastoris was induced by 0.5% (v/v) methanol at $30^{\circ}C$, whereas E. coli transformants expressed target protein in inclusion body after being induced by 1 mM IPTG at $37^{\circ}C$. The expression level increased dramatically to 250-300 mg/l supernatant of fermentation in the former and 80-100 mg/l in the latter. The LTB-6$\times$His were purified to 95% purity by affinity chromatography and characterized by SDS-PAGE and Western blot. Adjuvant activity of target protein was analyzed by binding ability with GMI gangliosides. The MW of LTB-6$\times$His expressed in P. pastoris was greater than that in E. coli, which was equal to the expected 11 kDa, possibly resulted from glycosylation by P. pastoris that would enhance the immunogenicity of co-administered antigens. These data demonstrated that P. pastoris producing heterologous LTB has significant advantages in higher expression level and in adjuvant activity compared with the homologous E. coli system.

• The Plant Pathology Journal
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• v.23 no.4
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• pp.239-244
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• 2007

MicroTom is a miniature tomato plants with various properties that make it as a model system for experiments in plant molecular biology. To extend its utility as a model plant to study a plant - bacterial wilt system, we investigated the potential of the MicroTom as a host plant of bacterial wilt caused by Ralstonia solanacearum. We compared the disease progress on standard tomato and MicroTom by two inoculation methods, root dipping and soil drenching, using a race 1 strain GMI1000. Both methods caused the severe wilting on MicroTom comparable to commercial tomato plant, although initial disease development was faster in root dipping. From the diseased MicroTom plants, the same bacteria were successfully reisolated using semiselective media to fulfill Koch's postulates. Race specific and isolate specific virulence were investigated by root dipping with 10 isolates of R. solanacearum isolated from tomato and potato plants. All of the tested isolates caused the typical wilt symptom on MicroTom. Disease severities by isolates of race 3 was below 50 % until 15 days after inoculation, while those by isolates of race 1 reached over 50% to death until 15 days. This result suggested that MicroTom can be a model host plant to study R. solanacearum - plant interaction.

• Journal of Microbiology
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• v.44 no.3
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• pp.293-300
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• 2006

The Escherichia coli heat-labile enterotoxin B subunit (HLT-B) is one of the most powerful mucosal immunogens and known mucosal adjuvants. However, the induction of high levels of HLT-B expression in E. coli has proven a difficult proposition. Therefore, in this study, the HLT-B gene was cloned from pathogenic E. coli and expressed as a fusion protein with GST (glutathion S-transferase) in E. coli BL2l (DE3), in an attempt to harvest a large quantity of soluble HLT-B. The culture conditions, including the culture media used, temperature, pH and the presence of lactose as an inducer, were all optimized in order to obtain an increase in the expression of soluble GST-rHLT-B. The biological activity of the purified rHLT-B was assayed in a series of GMI-ELISA experiments. The findings of these trials indicated that the yield of soluble recombinant GST-rHLT-B could be increased by up to 3-fold, as compared with that seen prior to the optimization, and that lactose was a more efficient alternative inducer than IPTG. The production of rHLT-B, at 92 % purity, reached an optimal level of 96 mg/l in a 3.7 L fermentor. The specific GM1 binding ability of the purified rHLT-B was determined to be almost identical to that of standard CTB.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.318-318
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• 2016

위성은 준 실시간으로 국토 전체의 관측과 미계측/비접근 지역의 관측도 가능하여 가뭄, 홍수 등 수재해와 관련된 분석 자료로 활용되고 있으며, 위성 기반의 수재해 모니터링 적용성에 대한 연구 또한 수행되고 있다. 위성에서 관측된 자료는 NASA, JAXA 등의 위성 관리 센터에서 알고리즘을 적용하여 인터넷으로 제공하고, 최근 K-water에서는 수자원분야의 위성활용을 위해 위성 자료 수집 시스템을 갖추어 Aqua/Terra MODIS, GPM, GCOM-W1 등의 위성 자료를 수집하고 있다. 위성 자료는 5분~16일 등의 다양한 주기로 제공되고 있으며, 자료 타입, 측정 시간 등의 간단한 정보만 파일명으로 표시되어 위성의 위치(경위도) 및 해당 지점의 위성 자료를 얻기 위해서는 위성 자료를 확인해야만 하는 번거로움이 따른다. 본 연구에서는 순차적으로 관측된 위성 자료의 시 공간적 속성정보를 추출하고 해당 정보를 영상과 함께 맵핑하여, 시간의 흐름에 따른 위성 궤도의 시각화 방안을 제시하였다. 위성 궤도의 시각화 방안으로 사용된 위성 자료는 Terra MODIS의 'MOD02SSH', GPM GMI 센서의 'GPROF' 자료 타입을 사용하였다. 'MOD02SSH'는 5분 동안 5km의 공간해상도로 측정한 자료가 1개의 파일이며, 'GPROF'는 5분 동안 4km의 공간해상도로 측정한다. 공전 주기의 검증을 위해 케플러의 제3법칙을 적용한 Terra 위성의 공전주기는 98.75분으로 계산되며, 위성 자료의 공전주기는 98.87분으로 나타난다. 검증 결과 약 0.12초의 오차가 발생하며, 정확한 위성 고도와 높은 해상도의 위성 자료를 통해 오차의 감소가 가능하다. 이를 통해 시각화 된 동적 시계열 이미지는 시간에 따른 위성 궤도의 정보를 추출 할 수 있다. 이는 수재해 정보시스템의 모니터링을 위해 사용 가능하고, 시간에 따른 위성 궤도 정보를 통하여 필요한 시간대의 위성 위치 정보, 해당 지점의 관측 자료를 효율적으로 수집하여 자료 수집을 위한 시간 단축이 가능하며, 사용자 또는 관리자를 위한 모니터링 수행 또한 효율적인 운영이 가능할 것으로 사료된다.

• Parasites, Hosts and Diseases
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• v.58 no.4
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• pp.421-430
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• 2020

This study aimed to investigate whether mass drug administration (MDA) intervention has an equivalent effect on reducing the prevalence and intensity of Schistosoma haematobium infection regardless of the baseline values. A repeated cross-sectional survey was performed targeting students of 12 primary schools in Al Jabalain and El Salam districts of White Nile State, Sudan, at both 1 week before and 8 months after the MDA. Prior to the baseline survey, schoolaged children in Al Jabalain had received MDA interventions twice in 4 years, while those in El Salam had not. The baseline prevalence was 9.1% in Al Jabalain and 35.2% in El Salam, which were reduced to 1.8% and 5.5% at 8 months after the MDA, respectively. The corresponding reduction rates were 80.3% and 84.4%, not significant difference between both districts. However, changes in the geometric mean intensity (GMI) of egg counts were significantly different between both districts. The baseline GMIs were 14.5 eggs per 10 ml of urine (EP10) in Al Jabalain and 18.5 EP10 in El Salam, which were reduced to 7.1 and 11.2 EP10 after treatment, respectively. The corresponding reduction rates were 51.0% and 39.5%. In conclusion, MDA interventions were found to bring about similar relative reduction in prevalence regardless of the baseline value; however, the relative reduction in infection intensity was more salient in the district with a low baseline value for both prevalence and intensity. This clearly points to the importance of repeated MDA interventions in endemic areas, which will eventually contribute to schistosomiasis elimination.

• Research in Plant Disease
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• v.30 no.2
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• pp.148-156
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• 2024

Ralstonia pseudosolanacearum, a plant pathogenic bacterium that can survive for a long time in soil and water, causes lethal wilt in the Solanaceae family. Sigma S is a part of the RNA polymerase complex, which regulates gene expression during bacterial stress response or stationary phase. In this study, we investigated the role of sigma S in R. pseudosolanacearum under stress conditions using a rpoS-defective mutant strain of R. pseudosolanacearum and its wild-type strain. The phenotypes of rpoS-defective mutant were complemented by introducing the original rpoS gene. There were no differences observed in bacterial growth rate and exopolysaccharide production between the wild-type strain and the rpoS mutant. However, the wild-type strain responded more sensitively to nutrient deficiency compared to the mutant strain. Under the nutrient deficiency, the rpoS mutant maintained a high bacterial viability for a longer period, while the viability of the wild-type strain declined rapidly. Furthermore, a significant difference in pH was observed between the culture supernatant of the wild-type strain and the mutant strain. The pH of the culture supernatant for the wild-type strain decreased rapidly during bacterial growth, leading to medium acidification. The rapid decline in the wild-type strain's viability may be associated with medium acidification and bacterial sensitivity to acidity during transition to the stationary phase. Interestingly, the rpoS mutant strain cannot utilize acetic acid, D-alanine, D-trehalose, and L-histidine. These results suggest that sigma S of R. pseudosolanacearum regulates the production or utilization of organic acids and controls cell death during stationary phase under nutrient deficiency.